|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | Development of hepatorenal syndrome in bile duct ligated rats显示文摘AIM: To evaluate in bile duct ligated rats whether there were progressive alterations of renal function without changes in histopathology. METHODS: Male Wistar rats were submitted to sham-surgery or bile duct ligation (BDL) and divided according to the post-procedure time (2, 4 and 6-wk). To determine renal function parameters, rats wereplaced in metabolic cages and, at the end of the experiment, blood and urine samples were obtained. Histology and hydroxyproline content were analyzed in liver and renal tissue. RESULTS: Rats with 2 wk of BDL increased free water clearance (P = 0.02), reduced urinary osmolality (P = 0.03) and serum creatinine (P = 0.01) in comparison to the sham group. In contrast, rats at 6 wk of BDL showed features of HRS, including signif icant increase in serum creatinine and reductions in creatinine clearance, water excretion and urinary sodium concentration. Rats with 4 wk of BDL exhibited an intermediate stage of renal dysfunction. Progressive hepatic f ibrosis according to post-procedure time was confirmed by histology. The increased levels of liver hydroxyproline contrasted with the absence of structural changes in the kidney, as assessed by histology and unchanged hydroxyproline content in renal tissue. CONCLUSION: Our data show that BDL produced progressive renal dysfunction without structural changes in the kidney, characterizing HRS. The present model will be useful to understand the pathophysiology of HRS. | Regina M Pereira Robson AS dos Santos Eduardo A Oliveira Virgínia HR Leite Filipi LC Dias Alysson S Rezende Lincoln P Costa Lucíola S Barcelos Mauro M Teixeira Ana Cristina Sim■es e Silva | 2008 | World Journal of Gastroenterology2008，14，28： | 2 |
| 3 | predictive factors for response to Lamivudine in chronic hepatitis B显示文摘 | Da Silva LC da Fonseca LE carrilho FJ | 2000 | Rev Inst Med Trop Sao Paulo2000，42，： | 1 |
| 4 | Combining ability for grain chemistry quality traits in a white oat dial- lelic cross显示文摘 | Crestani M Silveira Silveira SF Tessmann EW Mezzalira I Souza Luche H Silva JAG Gutkoski LC Carvalho FIF Oliveira AC | 2012 | Euphytica2012，185，1： | 1 |
| 5 | Metabolic acidosis in AIDS patients显示文摘 | Daher EF Cezar LC Silva GB Jr | 2009 | Arch Med Res2009，40，2： | 1 |
| 6 | Antibacterial activity of triterpene acids and semi-synthetic derivatives against oral pathogens显示文摘 | Scalon Cunha LC Andradee Silva ML Cardoso Furtado NA | 2007 | Z Naturfosch2007，62，910： | 1 |
| 7 | Ser- um cartilage oligomeric matrix protein(COMP) levels in knee os- teoarthritis in a brazilian population : clinical and radiologleal cor- relation 显示文摘 | FERNANDES FA PUCINELLI M LC DA SILVA NP | 2007 | Scand J Rheumatol2007，36，3： | 1 |
| 8 | Flavonoids and antiulcerogenic activity from Byrsonima crassa leaves extracts显示文摘 | Sannomiya M Fonseca VB da Silva MA Rocha LR Dos Santos LC Hiruma-Lima CA Souza Brito AR Vilegas W | | 0，，1： | 1 |
| 9 | Homocysteine concentrations and molecular analysis in patients with congenital heart defects显示文摘 | Galdieri LC Arrieta SR Silva CM | 2007 | Arch Med Res2007，38，2： | 1 |
| 10 | Thoracic surgery: risk factors for postoperative complications of lung resection显示文摘 | Fernandes EO Teixeira C Silva LC | 2011 | Rev Assoc Med Bras2011，57，3： | 1 |
| 11 | Survivin and Telomerase Expression in the uterine cervix of women with human papillomavirus-induced lesions显示文摘 | Barbosa LC da Silva ID Corra JC | 2011 | Int J Gynecol Cancer2011，21，1： | 1 |
| 12 | Use of ash forest biomass combustion as acidity correction and fertility of a humic cambisol显示文摘 | Silva FR da Albuquerque JA Gatiboni LC Costa A da | 2013 | Revista de Ciencias Agroveterinarias2013，12，3： | 1 |
| 13 | Comparative bioavailability study with two amiodarone tablet formulations administered with and without food in healthy subjects 显示文摘 | dos Santos Filho HO Ilha JO Silva LC | 2007 | Arzneimittelforschung2007，57，： | 1 |
| 14 | Montmoril onite clay based polyurethane nanocompos-ite as substrate for retinal pigment ep-ithelial cellgrowth显示文摘 | DA SILVA GR DA SILVA-CUNHA A JR VIEIRA LC | | 0，，： | 1 |
| 15 | Efficacy and tolerability of long - term therapy using high lamivudine dose for the treatment of chronic hepatitis B显示文摘 | Da Silva LC Pinho JR Sitnik R Da Fonseca LE Carrilho FJ | 2001 | J Gastroenterol2001，36，7： | 1 |
| 16 | Antibacterial activity of triterpene acids and semi-synthetic de- rivatives against oral pathogens 显示文摘 | Scalon Cunha LC Andradee Silva ML Cardoso Furtado NA | 2007 | Z Natufforseh C2007，62，910： | 1 |
| 17 | Homocysteine concentrations and molecular analysis in patients with congenital heart defects显示文摘 | Galdieri LC Arrieta SR Silva CM | 2007 | Arch Med Res2007，38，2： | 1 |
| 18 | Evaluation of the agreement by examiners according to classifications of third molars显示文摘 | Lima C J Silva LC Melo MR | 2012 | Med Oral Patol Oral Cir Bucal2012，17，2： | 1 |
| 19 | Regulatory T cell as atai^et for cancer therapy 显示文摘 | de Rezende LC Silva IV Rangel LB | 2010 | Arch Immunol Ther Exp ( Warsz)2010，58，3： | 1 |
| 20 | Participation of cannabinoid receptors in peripheral nociception induced by some NSAIDs显示文摘 | Silva LC Romero TR Guzzo LS | 2012 | Braz J Med Biol Res2012，45，12： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员