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| 1 | Effects of donor cells on in vitro development of cloned bovine embryos显示文摘The donor cells from different individuals and with different foreign genes introduced were investigated to determine their effects on the efficiency of somatic cell nuclear transfer (SCNT). The bovine ear fibroblast from different individuals was isolated, cultured, and then transfected with foreign genes to establish the stable cell lines, which were used as donor cells for nuclear transfer. The oocytes were obtained through ovum pick up operation. After in vitro maturation, the M II phase oocytes were selected as receptors for nuclear transfer. The reconstructed embryos were cultured in vitro and observed at 2 h, 48 h, and 7 days after transfer to assess the rate of fusion using cleaved and blastocyst as the parameters of SCNT efficiency. The donor cells from different individuals (04036, 06081, 06088, and 06129) had no obvious effect on the fusion and cleaved rate, whereas there was significant difference in the blastocyst rate (P<0.05), and the rate was 62.3%, 37.0%, 35.1%, and 15.6%, respectively. There was no significant difference among the rate of fusion, cleaved and blastocyst in donor cells with different foreign genes (P>0.05). It was concluded that the genetic background of the donor cells could affect the effi- ciency of SCNT, while the introduction of foreign genes into the donor cells had no obvious effect on the efficiency. This study provides useful information for the SCNT and would benefit in promoting the efficiency. | Jing Fu Pengfei Guan Leiwen Zhao Hua Li Shuzhen Huang Fanyi Zeng Yitao Zeng | 2008 | Journal of Genetics and Genomics2008，35，5： | 6 |
| 2 | Neuroprotective effect of Longshengzhi capsule following permanent middle cerebral artery occlusion in rats显示文摘Objective:Longshengzhi capsule(LSZC)is an optimized preparation based on the traditional Chinese Medicine formula Buyanghuanwu Decoction(BYHWD),and is approved by the China Food and Drug Administration for treating stroke-induced disability and vascular diseases.Herein,we examined the pharmacodynamics,anti-apoptotic and anti-oxidant actions,and potential mechanisms of action of LSZC following stroke in rats.Methods:Permanent middle cerebral artery occlusion(MCAO)was used as an ischemic stroke model.LSZC was administered intragastrically.We examined the survival rate,bodyweight,and neurological deficits in stroke rats.Brain infarct size was determined by 2,3,5-triphenyltetrazolium chloride staining.Brain pathology was examined using hematoxylin and eosin staining,Nissl staining,and TdT-mediated dUTP Nick-End Labeling staining.Malondialdehyde,catalase,superoxide dismutase,and glutathione levels were examined by commercial kits.Expression of Nrf2,heme oxygenase-1,Bax,Bcl-2,cleaved caspase-3,and caspase-3 proteins in brain tissue was measured by Western blot.Results:LSZC markedly improved the survival rate and bodyweight,and reduced infarct volume and neurological deficit scores,in MCAO stroke rats.LSZC also significantly attenuated oxidative stress,as indicated by decreased expression of malondialdehyde,and upregulation of Nrf2,heme oxygenase-1,catalase,superoxide dismutase,and glutathione.Moreover,LSZC significantly decreased apoptosis,including a decrease in Bax and cleaved caspase-3 expression,and an increase in Bcl-2,as well as a reduction in numbers of apoptotic neurons.Conclusion:LSZC treatment is neuroprotective against ischemic stroke,potentially via reducing oxidative stress and apoptosis.The Nrf2 and apoptotic signaling pathways may play important roles in the antioxidant and anti-apoptotic actions of LSZC. | Xiaoli Sun Shuzhen Guo Yong Wang Bangze Fu Junjie Liu Yili Zhang Ruocong Yang Chun Li Jian Gao Li Gao Wei Wang | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，1： | 4 |
| 3 | Plasma metabolomics combined with personalized diagnosis guided by Chinese medicine reveals subtypes of chronic heart failure显示文摘Background:Chronic heart failure(CHF)is characterized by insufficient blood supply from heart to meet the body’s metabolic demands.Integrating Western and traditional Chinese medicine to treat CHF has proved a validated therapeutic approach.In recent years,metabolomics has been regarded as a potential platform to provide biomarkers for diseasesubtypes.Objective:To examine 38 patients,combined NMR plasma metabolomics and traditional Chinese medicine diagnosis in order to identify diagnostic biomarkers for two CHF syndrome subtypes.Methods:After processing the spectra,orthogonal partial least square discriminant analysis was performed,and the contributing NMR signals were analyzed using Y-scrambling statistical validation with good reliability.Results:Plasma metabolic patterns of yin deficiency and yang deficiency patients were clearly discriminated.The yin-deficiency group had increased level of lactate,glycoprotein,lipoprotein and lower levels of glucose,valine and proline.The yang-deficiency group had higher levels of lactate,glycoprotein and pyruvic acid,and lower levels of glucose and lipoprotein.Potential biomarkers of CHF based on the two traditional Chinese medicine syndromes indicated alternative modes of metabolites and metabolic pathways in the disease,e.g.dysfunction of energy utilization and disturbance in fatty acids,amino acids.Conclusion:This study suggests that combining metabolomics with traditional Chinese medicine diagnosis can reveal metabolic signatures for CHF syndrome subtypes.The plasma metabolites identified might be of special clinical relevance for subtypes of CHF,which could lead to further understanding of mechanisms involved and an improvement in personalized treatment for CHF. | Juan Wang Shuzhen Guo Kuo Gao Qi Shi Bangze Fu Chan Chen Liangtao Luo Dong Deng Huihui Zhao Wei Wang | 2015 | Journal of Traditional Chinese Medical Sciences2015，2，2： | 2 |
| 4 | The Influence of Paraffinic Base Oil on Low-temperature Viscosity of Naphthenic Base Oil显示文摘与底油和 naphthenic 底在不同集体比率上油的 paraffinic 混合的润滑剂油的低温度的粘性被实验测试了。naphthenic 库油的性能上的 paraffinic 库油的影响被学习测试的油的低温度的粘性调查。润滑剂油的粘性在油混合与高粘性的 paraffinic 底油的一个增加的内容增加了。并且当在混合的 paraffinic 底油的内容是不足道的时,低温度的粘性少些被影响。为了为提出润滑减少费用,上油,根据润滑油的性质能遇见说明, paraffinic 底油的小部分能被增加进 naphthenic 底油。根据与 paraffinic 底油混合的油和 naphthenic 底油的低温度的粘性上的学习,一条基本规则为润滑油限制的准备被得出。 | Liu Shuzhen Fu Hongrui | 2010 | China Petroleum Processing & Petrochemical Technology2010，12，1： | 2 |
| 5 | A High Notch Pathway Activation Predicts Response to γ Secretase Inhibitors in Proneural Subtype of Glioma Tumor‐Initiating Cells显示文摘 | Norihiko Saito Jun Fu Siyuan Zheng Jun Yao Shuzhen Wang Diane D. Liu Ying Yuan Erik P. Sulman Frederick F. Lang Howard Colman Roel G. Verhaak W. K. Alfred Yung Dimpy Koul | 2014 | Stem Cells2014，，1： | 1 |
| 6 | An Analysis on Mental Health Education of College Students with Financial Difficulties显示文摘With the enrollment expansion of college and continuous reform of college education system and college charging system,the number of college students with financial difficulties is increasing. The college students with family financial difficulties not only bear the huge economic burden,but also bear the huge pressure of learning and mental stress,and they have many kinds of psychological problems including psychological distress of tension and anxiety,psychological conflict of self-respect and self-abasement,conflict of desire for interpersonal interaction and self-containment and strong vanity and dependence mentality. This paper puts forward the corresponding recommendations to solve these problems. | Shuzhen WANG Ling NI Chaoqiong LI Rui LANG Dan FU | 2017 | Asian Agricultural Research2017，9，6： | 1 |
| 7 | Effects of lactitol on intestinal microflora and plasma endotoxin in patients with chronic viral hepatitis显示文摘 | Chunlei Chen Lanjuan Li Zhouwen Wu Huguang Chen Shuzhen Fu | 2007 | Journal of Infection2007，54，： | 1 |
| 8 | Three novel umami peptides derived from the alcohol extract of the Pacific oyster(Crassostrea gigas):identification,characterizations and interactions with T1R1/T1R3 taste receptors显示文摘Oyster(Crassostrea gigas),the main ingredient of oyster sauce,has a strong umami taste.In this study,three potential umami peptides,FLNQDEEAR(FR-9),FNKEE(FE-5),and EEFLK(EK-5),were identified and screened from the alcoholic extracts of the oyster using nano-HPLC-MS/MS analysis,i Umami-Scoring Card Method(i Umami-SCM)database and molecular docking(MD).Sensory evaluation and electronic tongue analysis were further used to confirm their tastes.The threshold of the three peptides ranged from 0.38 to 0.55 mg/m L.MD with umami receptors T1R1/T1R3 indicated that the electrostatic interaction and hydrogen bond interaction were the main forces involved.Besides,the Phe592 and Gln853 of T1R3 were the primary docking site for MD and played an important role in umami intensity.Peptides with two Glu residues at the terminus had stronger umami,especially at the C-terminus.These results contribute to the understanding of umami peptides in oysters and the interaction mechanism between umami peptides and umami receptors. | Baifeng Fu Di Wu Shuzhen Cheng Xianbing Xu Ling Zhang Lishu Wang Heshanm REl-Seedi Hanxiong Liu Ming Du | 2024 | Food Science and Human Wellness2024，13，1： | 1 |
| 9 | An Empirical Analysis on Mental Health of Agricultural College Students显示文摘Based on the empirical survey of 2013-2016 college students in Northwest A&F University,we analyze the 9260 valid samples,and the survey involves emotional state,self-worth,psychological adjustment capacity and students’ attitude towards mental health education.On the basis of statistical data,we summarize the general situation of agricultural college students’ mental health,analyze the problems in agricultural college students’ psychological development and bring forward the feasible recommendations. | Dan FU Ning MA Shuzhen WANG | 2017 | Asian Agricultural Research2017，9，6： | 0 |
| 10 | Astragalus Salvia Granules to Benefit the Qi (Qishen Yiqi Keli) protects H9C2 cardiomyocytes by suppressing oxidative stress显示文摘Objective:To investigate the effects of Qishen Yiqi Keli(QSYQ;Astragalus Salvia Granules to Benefit the Qi)on ROS scavenging and inhibition of NADPH oxidase in an effort to identify new natural antioxidants.Methods:A total of 23 representative components in QSYQ were investigated,their effects on hydrogen peroxide induced ROS were assayed by dichlorofluorescein assay.Lucigenin chemiluminescence was adopted to test the effect on NADPH oxidase activity in H9C2 cardiomyocyte cells,and pyrogallol autoxidation was adopted to test the superoxide scavenging capacity.Results:Nine compounds in QSYQ could significantly inhibited H2O2-induced ROS increase compared with the model group(P<.05),including tanshinone I,salvianolic acid A,danshinone IIA,and cryptotanshinone from salvia root,luteolin from salvia root,harpagoside,harpagide,and angoroside C from scrophularia root.Nine compounds in QSYQ could significantly inhibited the production ofO2in H9C2 cell.Tanshinone I showed a lowest IC50 value 0.07 mM,the other including salvianolic acid A,cryptotanshinone,and salvianolic acid B,luteolin,isochlorogenic acid C,astragaloside IV,and glycyrrhetinic acid.Licochalcone A inhibited the autoxidation of pyrogallol at a low concentration,and tanshinone I showed no significant inhibitory effect from 2 mM to 20 mM.Conclusion:QSYQ had significant effects on ROS scavenging and inhibition of NADPH oxidase.Tanshinone I and salvianolic acid A are potential NADPH oxidase inhibitors. | Bangze Fu Shuzhen Guo Xue Yu Hong Chang Wei Wang | 2016 | Journal of Traditional Chinese Medical Sciences2016，3，3： | 0 |
| 11 | Xylan/chitosan composites prepared by an ionic liquid system with unique antioxidant properties显示文摘Antioxidant function and solubility in water are highly desirable in many applications of chitosan.In this paper,a xylan/chitosan composite was prepared in an ionic liquid system using glutaraldehyde as a crosslinking agent.The antioxidant activity,reducing capacity and metal ion chelating ability of the resulting composite were determined.The chemical structure and thermal stability were analyzed by FT-IR,XRD and TGA.The results showed that chitosan was successfully cross-linked with xylan by glutaraldehyde in the ionic liquid system.Compared to pure chitosan,the ABTS·+scavenging activity of the xylan/chitosan composite increased from 10.56%to 97.59%.After cross-linking with xylan by glutaraldehyde,the reducing power of xylan/chitosan composite increased from 0.054 to 2.109.The capacity of chelating metal ion of the composite also increased from 42.35%to 86.71%compared to that of pure chitosan.An underlying mechanism was proposed to account for the improvement of the chemical properties of the chitosan in the composite. | Hailong Gao Na Liu Shuzhen Ni Haixia Lin Yingjuan Fu | 2017 | Journal of Bioresources and Bioproducts2017，2，3： | 0 |
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