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| 1 | Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction显示文摘AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.METHODS: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QTAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction.RESULTS: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×107 and 1.67×107 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.L4×105 and 3.02×105 copies of HBV DNA per mL in the semen.CONCLUSION: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen. | Wei-Ping Qian Yue-Qiu Tan Ying Chen Ying Peng Zhi Li Guang-Xiu Lu Made C. Liu Hsiang-Fu Kung Ming-Ling He Li-Ka Shing | 2005 | World Journal of Gastroenterology2005，11，34： | 25 |
| 2 | ACSL4 is a predictive biomarker of sorafenib sensitivity in hepatocellular carcinoma显示文摘Sorafenib is the first-line treatment of advanced hepatocellular carcinoma(HCC).However,there is a lack of validated biomarkers to predict sorafenib sensitivity.In this study we investigated the role of ACSL4,a positive-activating enzyme of ferroptosis,in sorafenib-induced cell death and HCC patient outcome.We showed that ACSL4 protein expression was negatively associated with IC50 values of sorafenib in a panel of HCC cell lines(R=−0.952,P<0.001).Knockdown of ACSL4 expression by specific siRNA/sgRNA significantly attenuated sorafenib-induced lipid peroxidation and ferroptosis in Huh7 cells,and also rescued sorafenib-induced inhibition of xenograft tumor growth in vivo.We selected 29 HCC patients with surgery as primary treatment and sorafenib as postoperative adjunct therapy from a hospital-based cohort.A high proportion(66.7%)of HCC patients who had complete or partial responses to sorafenib treatment(according to the revised RECIST guideline)had higher ACSL4 expression in the pretreated HCC tissues,compared with those who had stable or progressed tumor growth(23.5%,P=0.029).Since ACSL4 expression was independent of sorafenib treatment,it could serve as a useful predictive biomarker.Taken together,this study demonstrates that ACSL4 is essential for sorafenib-induced ferroptosis and useful for predicting sorafenib sensitivity in HCC.This study may have important translational impacts in precise treatment of HCC. | Ji Feng Pei-zhi Lu Guang-zhi Zhu Shing Chung Hooi Yong Wu Xiao-wei Huang Hui-qi Dai Pan-hong Chen Zhong-jie Li Wen-jing Su Chuang-ye Han Xin-ping Ye Tao Peng Jing Zhou Guo-dong Lu | 2021 | Acta Pharmacologica Sinica2021，42，1： | 11 |
| 3 | Quantitative and Functional Phosphoproteomic Analysis Reveals that Ethylene Regulates Water Transport via the C-Terminal Phosphorylation of Aquaporin PIP2;1 in Arabidopsis显示文摘乙烯参予众多的细胞的事件和生物过程的规定,包括水损失,在枯萎的叶和花花瓣期间。多样的乙烯回答可以经由在蛋白质 phosphorylation/dephosphorylation 和 ubiquitin/26S 之间的动态相互影响被调整调停 proteasome 的蛋白质降级和朊酶劈开。探讨乙烯怎么通过叉形得多的发信号的小径改变蛋白质 phosphorylation,我们执行了 15 N 稳定的同位素对待空气、对待乙烯的乙烯感觉迟钝的 Arabidopsis 双 loss-of-function 异种 ein3-1/eil1-1 上的基于标签、微分、量的 phosphoproteomics 学习。识别的 Among535 非冗余的 phosphopeptides,二和四 phosphopeptides 起来 -- 并且由乙烯的 downregulated 分别地。aquaporin PIP2 的调整乙烯的 phosphorylation; 1 断然在叶与水流动率和水损失被相关。在有量的 proteomics 的联合的基因研究, immunoblot 分析,原物胀大/缩小实验,并且长叶表示两个的转基因的植物上的水损失试金野类型并且 S280A/S283A-mutated PIP2 ; 1 在里面 Col-0 和 ein3eil1 乙烯增加浇的基因 backgroundssuggest 在由在 PIP2 的 C 终点提高 S280/S283 phosphorylation 的 Arabidopsis 房间的运输率; 1 。未知 kinase 或磷酸酶活动可以参予 EIN3/EIL1 的细胞的功能的起始的起来规定独立人士。这发现贡献我们理解那通常在植物器官的收获以后的存储期间被观察调整乙烯的叶枯萎。 | Dongjin Qing Zhu Yang Mingzhe Li Wai Shing Wong Guangyu Guo Shichang Liu Hongwei Guo Ning Li | 2016 | Molecular Plant2016，9，1： | 6 |
| 4 | Catalytic conversion of lignocellulosic biomass into chemicals and fuels显示文摘In the search of alternative resources to make commodity chemicals and transportation fuels for a low carbon future,lignocellulosic biomass with over 180-billion-ton annual production rate has been identified as a promising feedstock.This review focuses on the state-of-the-art catalytic transformation of lignocellulosic biomass into value-added chemicals and fuels.Following a brief introduction on the structure,major resources and pretreatment methods of lignocellulosic biomass,the catalytic conversion of three main components,i.e.,cellulose,hemicellulose and lignin,into various compounds are comprehensively discussed.Either in separate steps or in one-pot,cellulose and hemicellulose are hydrolyzed into sugars and upgraded into oxygen-containing chemicals such as 5-HMF,furfural,polyols,and organic acids,or even nitrogen-containing chemicals such as amino acids.On the other hand,lignin is first depolymerized into phenols,catechols,guaiacols,aldehydes and ketones,and then further transformed into hydrocarbon fuels,bioplastic precursors and bioactive compounds.The review then introduces the transformations of whole biomass via catalytic gasification,catalytic pyrolysis,as well as emerging strategies.Finally,opportunities,challenges and prospective of woody biomass valorization are highlighted. | Weiping Deng Yunchao Feng Jie Fu Haiwei Guo Yong Guo Buxing Han Zhicheng Jiang Lingzhao Kong Changzhi Li Haichao Liu Phuc T.T.Nguyen Puning Ren Feng Wang Shuai Wang Yanqin Wang Ye Wang Sie Shing Wong Kai Yan Ning Yan Xiaofei Yang Yuanbao Zhang Zhanrong Zhang Xianhai Zeng Hui Zhou | 2023 | Green Energy & Environment2023，8，1： | 6 |
| 5 | Low effect‐site concentration of propofol target‐controlled infusion reduces the risk of hypotension during endoscopy in a T aiwanese population显示文摘 | Wen‐Hung Hsu Sophie SW Wang Hsiang‐Yao Shih Meng‐Chieh Wu Yi‐Yu Chen Fu‐Chen Kuo Hui‐Ying Yang Shun‐Li Chiu Koung‐Shing Chu Kuang‐I Cheng Deng‐Chyang Wu I‐Cheng Lu | 2013 | Journal of Digestive Diseases2013，，3： | 3 |
| 6 | Prognostic value of alcohol consumptionand some other dietary habits for survival in a cohort of Chinese men with lung cancer显示文摘Background:Alcohol consumption and some other dietary habits are thought to be associated with lung cancer incidence.However,the effects of these habits on lung cancer prognosis have been studied rarely.The purpose of this study was to address these gaps in knowledge.Methods:We studied a cohort of 1052 Chinese men in Hong Kong who were diagnosed with primary lung cancer.Cox proportional hazards models were used to determine the prognostic values of consumption of alcohol,fresh fruits or vegetables,meat,and fried or preserved food.Results:Compared with never drinkers,men who drank alcohol 1-3 days per week had a more favorable lung cancer prognosis(hazard ratio[HR]:0.82,95%confidence interval[CI]0.68-0.97);however,this survival advantage was not significant in men who drank alcohol more frequently(HR:0.91,95%CI 0.73-1.14).Compared with men who consumed preserved or fried food only occasionally,men who consumed these foods frequently had a higher risk of lung cancer mortality(HR:1.20,95%CI 1.00-1.42).Conclusions:Occasional consumption of alcohol was a favorable survival factor for Chinese men with lung cancer.However,this survival benefit did not exist for frequent drinkers of alcohol.Chinese men with lung cancer who were frequent consumers of fried or preserved food had a worse prognosis than those who consumed these foods only occasionally. | Wentao Li Lap Ah Tse Joseph S.K.Au Kai Shing Yu Feng Wang Ignatius Tak-sun Yu | 2017 | Chinese Journal of Cancer2017，36，7： | 3 |
| 7 | Brain delivering RNA-based therapeutic strategies by targeting mTOR pathway for axon regeneration after central nervous system injury显示文摘Injuries to the central nervous system(CNS)such as stroke,brain,and spinal cord trauma often result in permanent disabilities because adult CNS neurons only exhibit limited axon regeneration.The brain has a surprising intrinsic capability of recovering itself after injury.However,the hostile extrinsic microenvironment significantly hinders axon regeneration.Recent advances have indicated that the inactivation of intrinsic regenerative pathways plays a pivotal role in the failure of most adult CNS neuronal regeneration.Particularly,substantial evidence has convincingly demonstrated that the mechanistic target of rapamycin(mTOR)signaling is one of the most crucial intrinsic regenerative pathways that drive axonal regeneration and sprouting in various CNS injuries.In this review,we will discuss the recent findings and highlight the critical roles of mTOR pathway in axon regeneration in different types of CNS injury.Importantly,we will demonstrate that the reactivation of this regenerative pathway can be achieved by blocking the key mTOR signaling components such as phosphatase and tensin homolog(PTEN).Given that multiple mTOR signaling components are endogenous inhibitory factors of this pathway,we will discuss the promising potential of RNA-based therapeutics which are particularly suitable for this purpose,and the fact that they have attracted substantial attention recently after the success of coronavirus disease 2019 vaccination.To specifically tackle the blood-brain barrier issue,we will review the current technology to deliver these RNA therapeutics into the brain with a focus on nanoparticle technology.We will propose the clinical application of these RNA-mediated therapies in combination with the brain-targeted drug delivery approach against mTOR signaling components as an effective and feasible therapeutic strategy aiming to enhance axonal regeneration for functional recovery after CNS injury. | Ming-Xi Li Jing-Wen Weng Eric S.Ho Shing Fung Chow Chi Kwan Tsang | 2022 | Neural Regeneration Research2022，17，10： | 2 |
| 8 | Single vs twice daily G-CSF dose for peripheral blood stem cells harvest in normal donors and chil- dren with non-malignant diseases 显示文摘 | Lee V Li CK Shing MM | 2000 | Bone Marrow Transplant2000，25，9： | 1 |
| 9 | Solving the Puzzles of Structural Efficiency 显示文摘 | Sung - ko Li Yuk - shing Cheng | 2007 | European Journal of Operational Re- search2007，180，： | 1 |
| 10 | Upper bound for the first eigenvalue of algebraic submanifolds显示文摘 | Jean-Pierre Bourguignon Peter Li Shing Tung Yau | 1994 | Commentarii Mathematici Helvetici1994，，1： | 1 |
| 11 | Haematopoietic stem cell transplantation for thalassaemia in Chinese patients 显示文摘 | LI C K LEE V SHING M M | 2009 | Hong Kong Med J2009，15，33： | 1 |
| 12 | Production and characterisation of a novel chicken IgY antibody raised against C-terminal peptide from human thymidine kinase 1显示文摘 | Chuanjing Wu Rongjiang Yang Ji Zhou Shing Bao Li Zou Pinggan Zhang Yongrong Mao Jianping Wu Qimin He | 2003 | Journal of Immunological Methods2003，，1： | 1 |
| 13 | Synthesis and functional properties of end-dendronized oligo ( 9,9-diphenyl ) fluorenes 显示文摘 | Zhong Hui Li Man Shing Wong | 2006 | Org Let2006，8，7： | 1 |
| 14 | Mobile Human Airbag System for Fall Protection Using MEMS Sensors and Embedded SVM Classifier显示文摘 | HI Guangyi CHAN Cheung Shing LI Wen Jung | 2009 | IEEE Sensors2009，9，5： | 1 |
| 15 | On the parabolic kernel of the Schr?dinger operator显示文摘 | Peter Li Shing Tung Yau | 1986 | Acta Mathematica1986，，1： | 1 |
| 16 | On allocation of redundancies in two-component series systems显示文摘 | Peng Zhao Ping Shing Chan Long Li Hon Keung Tony Ng | 2013 | Operations Research Letters2013，，6： | 1 |
| 17 | Allocation of two redundancies in two‐component series systems显示文摘 | Peng Zhao Ping Shing Chan Long Li Hon Keung Tony Ng | 2013 | Naval Research Logistics2013，，7： | 1 |
| 18 | Synthesis and func- tional properties of end-dendronized oligo ( 9, 9-di- phenyl) fluorenes 显示文摘 | Zhong Hui Li Man Shing Wong | 2006 | Org Lett2006，8，7： | 1 |
| 19 | Second malignant neoplasms in childhood cancer survivors in a tertiary paediatric oncology centre in Hong Kong, China显示文摘背景童年癌症幸存者在这研究的第二个恶意的 neoplasms.The 目的开发的风险是在香港在一个第三级的儿科学的肿瘤学中心在童年癌症幸存者评估发生,风险因素和第二个恶意的瘤的结果,我们执行了的 China.Methods 有在威尔士医院,香港,在1984年5月之间的中国和2009年6月 .Case 的王子在孩子的癌症中心治疗的童年癌症的病人的回顾的评论病人记录 | SUN Wai-fun CHENG Frankie Wai-tsoi LEE Vincent LEUNGWing-kwan SHING Ming-kong YUEN Patrick Man-pan LI Chi-kong | 2011 | Chinese Medical Journal2011，，22： | 1 |
| 20 | On the parabolic kernel of the Schr?dinger operator显示文摘 | Peter Li Shing Tung Yau | 1986 | Acta Mathematica1986，，1： | 1 |
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