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| 1 | Expression changes and bioinformatic analysis of Wallerian degeneration after sciatic nerve injury in rat显示文摘Wallerian degeneration (WD) remains an important research topic. Many genes are differentially expressed during the process of WD, but the precise mechanisms responsible for these differentiations are not completely understood. In this study, we used microarrays to analyze the expression changes of the distal nerve stump at 0, 1, 4, 7, 14, 21 and 28 days after sciatic nerve injury in rats. The data revealed 6 076 differentially-expressed genes, with 23 types of expression, specifically enriched in genes associated with nerve development and axonogenesis, cytokine biosynthesis, cell differentiation, cytokine/chemokine production, neuron differentiation, cytokinesis, phosphorylation and axon regeneration. Kyoto Encyclopedia of Genes and Genomes pathway analysis gave findings related mainly to the MAPK signaling pathway, the Jak-STAT signaling pathway, the cell cycle, cytokine-cytokine receptor interaction, the p53 signaling pathway and the Wnt signaling pathway. Some key factors were NGF, MAG, CNTF, CTNNA2, p53, JAK2, PLCB1, STAT3, BDNF, PRKC, collagen II, FGF, THBS4, TNC and c-Src, which were further validated by real-time quantitative PCR, Western blot, and immunohistochemistry. Our findings contribute to a better understanding of the functional analysis of differentially-expressed genes in WD and may shed light on the molecular mechanisms of nerve degeneration and regeneration. | Dengbing Yao Meiyuan Li Dingding Shen Fei Ding Shibi Lu Qing Zhao Xiaosong Gu | 2013 | Neuroscience Bulletin2013，29，3： | 18 |
| 2 | A neurotrophic peptide-functionalized self-assembling peptide nanofiber hydrogel enhances rat sciatic nerve regeneration显示文摘神经指导水管(NGC ) 是为外部神经新生的自体同源的神经的一种潜在的选择。有希望的治疗学的策略是用物理或化学的指导暗示修改神经指导水管 intraluminal 微型环境。在这研究,神经营养的 peptide-functionalized 能在 vitro 支持 PC12 细胞粘附,增长,和 neuronal 区别的自我装配的肽 nanofiber hydrogel 是在一个空 chitosan 试管(hCST ) 的腔的 prefilled 在一只老鼠加速 axonal 新生臀部的神经缺点模型。functionalized 自我装配的肽被介绍神经营养的肽开发(RGI, RGIDKRHWNSQ ) 源于导出大脑的神经营养的因素(BDNF ) 到自我装配的肽 RADA16 的 C 终点 -- 我(交流 --(RADA )4-CONH2) 。词法,组织学, electrophysiological,和功能的分析证明 RGI-functionalized,自我装配,肽 nanofiber hydrogel RAD/RGI 能生产显著地与提高的 re-myelination 和马达改进了 axonal 新生的神经营养的微型环境功能的恢复。 | Jiaju Lu Xun Sun Heyong Yin Xuezhen Shen Shuhui Yang Yu Wang Wenli Jiang Yue Sun Lingyun Zhao Xiaodan Sun Shibi Lu Antonios G. Mikos Jiang Peng Xiumei Wang | 2018 | Nano Research2018，11，9： | 6 |
| 3 | Gene expression profiling of the rat sciatic nerve in early Wallerian degeneration after injury显示文摘Wallerian degeneration is an important area of research in modern neuroscience.A large number of genes are differentially regulated in the various stages of Wallerian degeneration,especially during the early response.In this study,we analyzed gene expression in early Wallerian degeneration of the distal nerve stump at 0,0.5,1,6,12 and 24 hours after rat sciatic nerve injury using gene chip microarrays.We screened for differentially-expressed genes and gene expression patterns.We examined the data for Gene Ontology,and explored the Kyoto Encyclopedia of Genes and Genomes Pathway.This allowed us to identify key regulatory factors and recurrent network motifs.We identified 1 546 differentially-expressed genes and 21 distinct patterns of gene expression in early Wallerian degeneration,and an enrichment of genes associated with the immune response,acute inflammation,apoptosis,cell adhesion,ion transport and the extracellular matrix.Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed components involved in the Jak-STAT,ErbB,transforming growth factor-β,T cell receptor and calcium signaling pathways.Key factors included interleukin-6,interleukin-1,integrin,c-sarcoma,carcinoembryonic antigen-related cell adhesion molecules,chemokine(C-C motif) ligand,matrix metalloproteinase,BH3 interacting domain death agonist,baculoviral IAP repeat-containing 3 and Rac.The data were validated with real-time quantitative PCR.This study provides a global view of gene expression profiles in early Wallerian degeneration of the rat sciatic nerve.Our findings provide insight into the molecular mechanisms underlying early Wallerian degeneration,and the regulation of nerve degeneration and regeneration. | Dengbing Yao Meiyuan Li Dingding Shen Fei Ding Shibi Lu Qin Zhao Xiaosong Gu | 2012 | Neural Regeneration Research2012，7，17： | 5 |
| 4 | Immune characterization of mesenchymal stem cells in human umbilical cord Wharton’s jelly and derived cartilage cells显示文摘 | Shuyun Liu Mei Yuan Kedong Hou Li Zhang Xifu Zheng Bin Zhao Xiang Sui Wenjing Xu Shibi Lu Quanyi Guo | 2012 | Cellular Immunology (-)2012，，1： | 1 |
| 5 | Role of mesenchymal stem cells in bone regeneration and fracture repair: a review显示文摘 | Xin Wang Yu Wang Wenlong Gou Qiang Lu Jiang Peng Shibi Lu | 2013 | International Orthopaedics2013，，12： | 1 |
| 6 | Role of stem cells in the regeneration and repair of peripheral nerves显示文摘 | Zhiwu Ren Yu Wang Jiang Peng Qing Zhao Shibi Lu | 2012 | Reviews in the Neurosciences2012，，： | 1 |
| 7 | Comparative study of oxidative stress induced by sand flower and schistose nanosized layered double hydroxides in N2a cells显示文摘镁铝分层的双氢氧化物(Mg/Al-LDH ) nanoparticles 因为他们的低毒性和合适的 biocompatibility,作为药交货系统有强壮的潜在的申请。然而,很少研究在神经细胞上描述了这些氢氧化物的词法效果。现在的学习比较不同集中导致的氧化压力(即, 0, 50, 100, 200, 400,和 800 g/mL ) 在老鼠 neuroblastoma 房间(N2a ) 的沙花和薄片 nano-Mg/Al-LDHs 当这些房间为 24 和 48 h 被暴露时。房间生存能力被 MTT 试金检测,并且反应的氧种类(ROS ) 的生产,谷胱甘肽(GSH ) ,和 malondialdehyde (MDA ) 被监视评估氧化损坏。结果建议了那沙花 nano-LDHs,在适当集中(不到 200 g/mL ) ,特别那些在尺寸的大约 100200 nm,别在 N2a 房间上导致有害效果。 | YH Lu Biao Yan Xudong Liu Yuchao Zhang Shibi Zeng Hao Hu Rong Xiang Yu Xu Ying Yu Xu Yang | 2015 | Frontiers in Biology2015，10，3： | 1 |
| 8 | Recellularized nerve allografts with differentiated mesenchymal stem cells promote peripheral nerve regeneration显示文摘 | Yu Wang Zhe Zhao Zhiwu Ren Bin Zhao Li Zhang Jifeng Chen WenJing Xu Shibi Lu Qing Zhao Jiang Peng | 2012 | Neuroscience Letters2012，，1： | 1 |
| 9 | A cartilage ECM-derived 3-D porous acellular matrix scaffold for in vivo cartilage tissue engineering with PKH26-labeled chondrogenic bone marrow-derived mesenchymal stem cells显示文摘 | Qiang Yang Jiang Peng Quanyi Guo Jingxiang Huang Li Zhang Jun Yao Fei Yang Shenguo Wang Wenjing Xu Aiyuan Wang Shibi Lu | 2008 | Biomaterials2008，，： | 1 |
| 10 | EXPERIMENTAL STUDIES OF THE EFFECTS OF PRESTRESS显示文摘EXPERIMENTALSTUDIESOFTHEEFFECTSOFPRESTRESSonintactChanineTibiaBoneStructureLiuBaowei,LuShibi,WangJifangDept.ofOrthop.ofGenera... | on intact Chanine Tibia Bone StructureLiu Baowei,Lu Shibi,Wang JifangDept.of Orthop. of General Hospital of PLA China,Beijing 100853,China | 1996 | Chinese Journal of Biomedical Engineering(English Edition)1996，5，4： | 0 |
| 11 | Chondroitinase ABC improves recovery of long sciatic nerve defects显示文摘Sciatic nerves from allogeneic Sprague-Dawley rats were pretreated with chondroitinase ABC and were used to bridge damaged sciatic nerves in Wistar rats. Chondroitin sulfate proteoglycans were removed from the chemically extracted acellular nerves. At 3 months after grafting, the footplate pinch test result was positive in the Wistar rats. Autotomy scores decreased, and increased muscular contraction tension appeared when triceps surae muscles were stimulated. In addition, the recovery rate of wet triceps surae muscle weight increased, and the distal segment of the chondroitinase ABC-treated graft exhibited Schwann cells next to the nerve fibers. These results suggested that chondroitinase ABC pretreatment enhanced repair of long nerve defects via acellular nerve grafting. | Hailong Yu Liangbi Xiang Wenjing Xu Bin Zhao Yu Wang Jiang Peng Shibi Lu | 2012 | Neural Regeneration Research2012，7，1： | 0 |
| 12 | Protein expression of sensory and motor nerves Two-dimensional gel electrophoresis and mass spectrometry显示文摘The present study utilized samples from bilateral motor branches of the femoral nerve,as well as saphenous nerves,ventral roots,and dorsal roots of the spinal cord,to detect differential protein expression using two-dimensional gel electrophoresis and nano ultra-high performance liquid chromatography electrospray ionization mass spectrometry tandem mass spectrometry techniques.A mass spectrum was identified using the Mascot search.Results revealed differential expression of 11 proteins,including transgelin,Ig kappa chain precursor,plasma glutathione peroxidase precursor,an unnamed protein product(gi|55628),glyceraldehyde-3-phosphate dehydrogenase-like protein,lactoylglutathione lyase,adenylate kinase isozyme 1,two unnamed proteins products(gi|55628 and gi|1334163),and poly(rC)-binding protein 1 in motor and sensory nerves.Results suggested that these proteins played roles in specific nerve regeneration following peripheral nerve injury and served as specific markers for motor and sensory nerves. | Zhiwu Ren Yu Wang Jiang Peng Li Zhang Wenjing Xu Xiangdang Liang Qing Zhao Shibi Lu | 2012 | Neural Regeneration Research2012，7，5： | 0 |
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