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| 1 | Methods for the extraction and RNA profiling of exosomes显示文摘AIM:To develop protocols for isolation of exosomes and characterization of their RNA content.METHODS:Exosomes were extracted from He La cell culture media and human blood serum using the Total exosome isolation(from cell culture media)reagent,and Total exosome isolation(from serum)reagent respectively.Identity and purity of the exosomes was confirmed by Nanosight?analysis,electron microscopy,and Western blots for CD63 marker.Exosomal RNA cargo was recovered with the Total exosome RNA and protein isolation kit.Finally,RNA was profiled using Bioanalyzer and quantitative reverse transcriptionpolymerase chain reaction(q RT-PCR)methodology.RESULTS:Here we describe a novel approach for robust and scalable isolation of exosomes from cell culture media and serum,with subsequent isolation and analysis of RNA residing within these vesicles.The isolation procedure is completed in a fraction of the time,compared to the current standard protocols utilizing ultracentrifugation,and allows to recover fully intact exosomes in higher yields.Exosomes were found tocontain a very diverse RNA cargo,primarily short sequences 20-200 nt(such as mi RNA and fragments of m RNA),however longer RNA species were detected as well,including full-length 18S and 28S r RNA.CONCLUSION:We have successfully developed a set of reagents and a workflow allowing fast and efficient extraction of exosomes,followed by isolation of RNA and its analysis by q RT-PCR and other techniques. | Emily Zeringer Mu Li Tim Barta Jeoffrey Schageman Ketil Winther Pedersen Axl Neurauter Susan Magdaleno Robert Setterquist Alexander V Vlassov | 2013 | World Journal of Methodology2013，3，1： | 5 |
| 2 | Exosomes:current knowledge of their composition,biological functions,anddiagnostic and therapeutic potentials显示文摘 | Vlassov A V Magdaleno S Setterquist R | 2012 | Biochim Biophys Acta2012，1820，7： | 1 |
| 3 | Endophthalmitis after cataract surgery:Risk factors relating to technique and events of the operation and patient history:A retrospective case-control study显示文摘 | Montan PG Koranyi G Setterquist HE | 1998 | Ophthalmology1998，105，12： | 1 |
| 4 | Exosome , currrent knowledge of their composition, biological function, and diagnostic and therapeutic potentials 显示文摘 | Vlassov AV Magdaleno S Setterquist R | 2012 | Biochim Biophys Acta2012，1820，7： | 1 |
| 5 | Exosomes: current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials显示文摘 | Vlassov A V Magdaleno S Setterquist R | 2012 | Biochim Biopto s Acta2012，1820，7： | 1 |
| 6 | Exosomes: Current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials 显示文摘 | Vlassdv A V Magdaleno S Setterquist R | 2012 | Biochim Biophys Acta2012，1820，7： | 1 |
| 7 | Exosomes: cur- rent knowledge of their composition, biological functions, and di- agnostic and therapeutic potentials 显示文摘 | Vlassov AV Magdaleno S Setterquist R | 2012 | Biochim Biophys Acta2012，1820，7： | 1 |
| 8 | Are swine workers in the United States at increased risk of infection with zoonotic influenza virus显示文摘 | Myers KP Olsen CW Setterquist SF etal | 2006 | ? Clin Infect Dis2006，42，1： | 1 |
| 9 | Exosomes:Current knowledge of their composition,biological functions,and diagnostic and therapeutic potentials显示文摘 | Vlassov A Magdaleno S Setterquist R | 2012 | Biochim Biophys Acta2012，1820，7： | 1 |
| 10 | Exosomes: Current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials显示文摘 | Alexander V. Vlassov Susan Magdaleno Robert Setterquist Rick Conrad | 2012 | BBA - General Subjects2012，，7： | 1 |
| 11 | Exosomes:current knowledge of their composition,biological functions,and diagnostic and therapeutic potentials显示文摘 | Vlassov AV Magdaleno S Setterquist R | 2012 | Biochim Biophys Acta2012，1820，7： | 1 |
| 12 | Comparison of Mycobacteria growth indicator tube with BACTEC460 for detection and reco-very of Mycobacteria from clinical specimens显示文摘 | Badak ZF Kiska DL Setterquist S | 1996 | J Clin Microbiol1996，34，9： | 1 |
| 13 | Exosomes: current knowledge of their composition,biological functions, and diagnostic and therapeutic potentials 显示文摘 | Vlassov AV Magdaleno S Setterquist R | 2012 | Biochim Bio phys Aeta2012，1820，7： | 1 |
| 14 | Exosomes: current knowledge of their composition, biological functions, and diagnos- tic and therapeutic potentials显示文摘 | Vlassov AV Magdaleno S Setterquist R | 2012 | Bioehim Biophys Acta2012，1820，7： | 1 |
| 15 | Comparison of mycobacteria growth indicator tube with BACTEC 460 for detection and recovery of mycobacteria form clinical specimens 显示文摘 | Kiska DL Setterquist S | 1996 | J Clin Microbiol1996，34，9： | 1 |
| 16 | Endophthalmitis after cataract surgery: risk factors relating to technique and events of the operation and patient history: a retrospective case-control study 显示文摘 | MONTAN P G KORANYI G SETTERQUIST H E | 1998 | Ophthalmology1998，105，12： | 1 |
| 17 | Diagnostic probes targeting the major sperm protein gene that may be useful in the molecular identification of nematodes 显示文摘 | Setterquist R A Smith G K Jones R | 1996 | Joumal of Nema- tology1996，28，： | 1 |
| 18 | Multi-year study of human metapneumovirus infection at a large US Midwestern Medical Referral Center显示文摘 | Gray GC Capuano AW Setterquist SF | | 0，，04： | 1 |
| 19 | Exosomes : current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials显示文摘 | Vlassov AV Magdaleno S Setterquist R | 2012 | Biochim Biophys Acta2012，1820，7： | 1 |
| 20 | Prophylactic intracameral cefuroxime:Evaluation of safety and kinetics in cataract surgery显示文摘 | Montan PG Wejde G Setterquist H | 2002 | Cataract Refract Surg2002，28，6： | 1 |
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