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| 1 | Diarylheptanoid-myricanone isolated from ethanolic extract of Myrica cerifera shows anticancer effects on HeLa and PC3 cell lines:signalling pathway and drug-DNA interaction显示文摘OBJECTIVE:To test if myricanone(C_(21)H_(24)O_5),a cyclic diarylheptanoid,has anticancer effects on two different cancer cell lines HeLa and PC3.The present study was conducted with a note on the drug-DNA interaction and apoptotic signalling pathway.METHODS:Several studies like cytotoxicity,nuclear damage,annexin-V-fluorescein isothiocyanate(FITC)/propidium iodide(Pl)-labelled apoptotic assay and cell cycle arrest,immunoblot and reverse transcriptase-polymerase chain reaction(RT-PCR)were used following standard protocols.Circular dichroism(CD)spectroscopy was also done to evaluate whether myricanone effectively interacted with DNA to bring about conformational changes that could strongly inhibit the cancer cell proliferation.RESULTS:Myricanone showed a greater cytotoxic effect on PC3 cells than on HeLa cells.Myricanone promoted G_0/G_1 arrest in HeLa cells and S phase arrest in PC3 cells.Nuclear condensation and annexin V-FITC/PI studies revealed that myricanone promoted apoptotic cell death.CD spectroscopic data indicated that myricanone had an interaction with calf thymus DNA that changed DNA structural conformation.RT-PCR and immunoblot studies revealed that myricanone activated the apoptotic signalling cascades through down-regulation of transcription factors like nuclear factor-κB(NF-κB)(p65),and signal transducers and activators of transcription 3(STAT3);cell cycle regulators like cyclin D1,and survivin and other signal proteins like Bcl-2 and up-regulation of Bax,caspase-9 and caspase-3.CONCLUSION:Myricanone induced apoptosis in both types of cancer cells by triggering caspase activation,and suppression of cell proliferation by down-regulation of NF-κB and STAT3signalling cascades,which makes it a suitable candidate for possible use in the formulation of therapeutic agent for combating cancer. | Avijit Paul Sreemanti Das Jayeeta Das Asmita Samadder Kausik Bishayee Ratan Sadhukhan Anisur Rahman Khuda-Bukhsh | 2013 | Journal of Integrative Medicine2013，11，6： | 4 |
| 2 | Current molecular methods for the detection of hepatitis C virus in high risk group population:A systematic review显示文摘Hepatitis C virus(HCV) is an emerging infection worldwide and the numbers of persons infected are increasing every year. Poor blood transfusion methods along with unsafe injection practices are potential sources for the rapid spread of infection. Early detection of HCV is the need of the hour especially in high riskgroup population as these individuals are severely immunocompromised. Enzyme Immunoassays are the most common detection techniques but they provide no evidence of active viremia or identification of infected individuals in the antibody-negative phase and their efficacy is limited in individuals within high risk group population. Molecular virological techniques have an important role in detecting active infection with utmost specificity and sensitivity. Technologies for assessment of HCV antibody and RNA levels have improved remarkably, as well as our understanding of how to best use these tests in patient management. This review aims to give an overview of the different serological and molecular methods employed in detecting HCV infection used nowadays. Additionally, the review gives an insight in the new molecular techniques that are being developed to improve the detection techniques particularly in High Risk Group population who are severely immunocompromised. | Rushna Firdaus Kallol Saha Aritra Biswas Provash Chandra Sadhukhan | 2015 | World Journal of Virology2015，4，1： | 4 |
| 3 | An experimental and theoretical investigation on torrefaction of a large wet wood particle显示文摘 | Prabir Basu Anup Kumar Sadhukhan Parthapratim Gupta | 2014 | Bioresource Technology2014，159，2： | 1 |
| 4 | Crack formation in composites through a spring model显示文摘 | Sadhukhan S Dutta T Tarafdar S | | 0，，： | 1 |
| 5 | Microarray gene expression profiling and analysis in renal cell carcinoma显示文摘 | Liou L S Shi T Duan Z H Sadhukhan P Der S D Novick A A | 2004 | BMC Urol2004，4，： | 1 |
| 6 | Inhibitory action of tea extracts and(-)-epigallocatechin gallate on cell proliferation显示文摘 | LEA M A XIAO Q SADHUKHAN A K | 1993 | Proc Annu Meet Am Assoc Cancer Res1993，34，4： | 1 |
| 7 | Negative adsorption of inorganic salts, sucrose and urea at solid-iiquid interfaces 显示文摘 | Nag A Sadhukhan B Chattoraj D K | 1987 | Colloids and Surfaces1987，23，12： | 1 |
| 8 | Modelling of combustion characteristics of high ash coal char particles at high pressure: Shrinking reactive core model显示文摘 | SADHUKHAN A K GUPTA P SAHA R K | 2010 | Fuel2010，89，1： | 1 |
| 9 | Modelling of pyrolysis of coal–biomass blends using thermogravimetric analysis显示文摘 | Anup Kumar Sadhukhan Parthapratim Gupta Tripurari Goyal Ranajit Kumar Saha | 2008 | Bioresource Technology2008，，17： | 1 |
| 10 | Dual inhibi- tion of DNA topoisomerases of leishmania donovani by novel indolyl quinolines 显示文摘 | Ray S Sadhukhan P K Mandal N B | 1997 | Biochem Biophys Res Com- mun1997，230，1： | 1 |
| 11 | Characterization of porous structure of coal char from a single devolatilized coal particle:coal combustion in a fluidized bed显示文摘 | Sadhukhan A K Gupta P Saha R K | 2009 | Fuel Processing Technology2009，90，5： | 1 |
| 12 | Evaluation of PINK1 variants in Indian Parkinson's disease patients显示文摘 | Biswas A Sadhukhan T Majumder S | 2010 | Parkinsonism Relat Disord2010，16，3： | 1 |
| 13 | Modelling of pyrolysis of coal-biomass blends using thermogravimetric analysis显示文摘 | SADHUKHAN A K GUPTA P GOYAL T | | 0，，17： | 1 |
| 14 | Computer based thermodynamic properties of ammonia-water mixture for the analysis of power and refrigeration cycles 显示文摘 | SADHUKHAN K CHOWDHURI A K MANDAL B K | 2012 | International Journal of Thermodynamics2012，15，3： | 1 |
| 15 | Modelling of pyrolysis of coal-biomass blends using thermogravimetrie analysis显示文摘 | Sadhukhan A K Gupta P Goyal T | 2008 | Bioresouree Technology2008，99，17： | 1 |
| 16 | Discrete age - structured population model with age dependent harvesting and its stability analysis 显示文摘 | Sadhukhan D Mondal B Maiti M | 2008 | Applied Mathematics and Computation2008，，12： | 1 |
| 17 | Integration of bioethanol as an in-process material in biorefineries using mass pinch analysis显示文摘 | Elias Martinez-Hernandez Jhuma Sadhukhan Grant M. Campbell | 2013 | Applied Energy2013，，： | 1 |
| 18 | Near-infrared offers benefits and challenges in gasoline analysis 显示文摘 | Difoggio R Sadhukhan M Ranc M L | 1993 | J Oil & Gas1993，，5： | 1 |
| 19 | Techno-economic perform- ante analysis of bio-oil based Fischer-Tropsch and CHP synthesis platform显示文摘 | NG K S SADHUKHAN J | 2011 | Biomass and Bioenergy2011，35，7： | 1 |
| 20 | Facile bulk production of highly blue fluorescent graphitic carbon nitride quantum dots and their application as highly selective and sensitive sensors for the detection of mercuric and iodide ions in aqueous media显示文摘 | BARMAN S SADHUKHAN M | 2012 | Jour- nal of Materials Chemistry2012，22，41： | 1 |
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