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| 1 | Structure of MERS-CoV spike receptor-binding domain complexed with human receptor DPP4显示文摘最近的尖铁 glycoprotein (S) 鉴别中东呼吸症候群 coronavirus (MERS-CoV ) 指向细胞的受体, dipeptidyl peptidase 4 (DPP4 ) 。顺序比较和当模特儿的分析在病毒的尖铁上揭示了一个通常认为的受体绑定领域(RBD ) ,它调停这个相互作用。我们报导 3.0 Å MERS-CoV RBD 的决定水晶结构跳了到人的 DPP4 的细胞外的领域。我们的结果证明 MERS-CoV RBD 由一个核心和受体绑定子域组成。受体绑定子域与 DPP4 β 交往;推进器然而并非它的内在的 hydrolase 领域。MERS-CoV RBD 和相关 SARS-CoV RBD 分享他们的核心子域的结构的类似的高度,但是在受体绑定子域是尤其是分叉的。Mutagenesis 研究在为到 DPP4 和入口的病毒的绑定是批评的进目标房间的受体绑定子域识别了几关键残余。在在 MERS-CoV RBD 和 DPP4 之间的接口的原子细节提供病毒和受体相互作用的结构的理解,它能对 MERS-CoV 感染指导治疗学和疫苗的开发。 | Nianshuang Wang Xuanling Shi Liwei Jiang Senyan Zhang Dongli Wang Pei Tong Dongxing Guo Lili Fu Ye Cui Xi Liu Kelly C Arledge Ying-Hua Chen Linqi Zhang Xinquan Wang | 2013 | Cell Research2013，23，8： | 26 |
| 2 | Phosphorylcholine polymer nanocapsules prolong the circulation time and reduce the immunogenicity of therapeutic proteins显示文摘蛋白质治疗,治疗学的蛋白质在那里被交付对待混乱,为治疗疾病被认为最安全、很直接的途径。然而,它的应用被有效策略的少量高度为在他们的管理以后在 vivo 交付蛋白质和治疗学的蛋白质的快速的清理限制。这里,我们表明能显著地延长治疗学的蛋白质的发行量时间以及最小化他们的 immunogenicity 的新奇策略。这被与抵抗蛋白质吸附的 crosslinked phosphorylcholine 聚合物的薄层包含单个蛋白质分子完成。通过广泛的细胞的研究,我们证明 crosslinked phosphorylcholine 聚合物壳有效地阻止包含的蛋白质是由巨噬细胞的 phagocytosed,它在 vivo 在 nanoparticles 的清理起一个必要作用。而且,聚合物壳阻止包含的蛋白质被有免疫力的房间识别。作为结果,对治疗学的蛋白质的有免疫力的回答有效地被压制。这个工作描述一个可行方法延长循环时间并且减少治疗学的蛋白质的 immunogenicity,它可以在多样的疾病的治疗支持新奇蛋白质治疗的发展和申请。 | Sheng Liang Yang Liu Xin Jin Gan Liu Jing Wen Linlin Zhang Jie Li Xubo Yuan Irvin S.Y. Chen Wei Chen Hui Wang Linqi Shi Xinyuan Zhu Yunfeng Lu | 2016 | Nano Research2016，9，4： | 11 |
| 3 | Synthesis of Fe_3O_4@SiO_2@polymer nanoparticles for controlled drug release显示文摘Novel multifunctional nanoparticles containing a magnetic Fe3O4@SiO2 sphere and a biocompatible block copolymer poly(ethylene glycol)-b-poly(aspartate)(PEG-b-PAsp) were prepared.The silica coated on the superparamagnetic core was able to achieve a magnetic dispersivity,as well as to protect Fe3O4 against oxidation and acid corrosion.The PAsp block was grafted to the surface of Fe3O4@SiO2 nanoparticles by amido bonds,and the PEG block formed the outermost shell.The anticancer agent doxorubicin(DOX) was loaded into the hybrid nanoparticles via an electrostatic interaction between DOX and PAsp.The release rate of DOX could be adjusted by the pH value. | WU ChengLin,HE Huan,GAO HongJun,LIU Gan,MA RuJiang,AN YingLi & SHI LinQi Key Laboratory of Functional Polymer Materials,Ministry of Education Institute of Polymer Chemistry,Nankai University,Tianjin 300071,China | 2010 | Science China Chemistry2010，53，3： | 9 |
| 4 | Injectable dual glucose-responsive hydrogel-micelle composite for mimicking physiological basal and prandial insulin delivery显示文摘For type 1 and advanced type 2 diabetic patients, insulin replacement therapy with simulating on-demand prandial and basal insulin secretion is the best option for optimal glycemic control. However, there is no insulin delivery system yet could mimic both controlled basal insulin release and rapid prandial insulin release in response to real-time blood glucose changes. Here we reported an artificial insulin delivery system, mimicking physiological basal and prandial insulin secretion, to achieve real-time glycemic control and reduce risk of hypoglycemia. A phenylboronic acid(PBA)/galactosyl-based glucose-responsive insulin delivery system was prepared with insulin-loaded micelles embedded in hydrogel matrix. At the hyperglycemic state, both the hydrogel and micelles could swell and achieve rapid glucose-responsive release of insulin, mimicking prandial insulin secretion.When the glucose level returned to the normal state, only the micelles partially responded to glucose and still released insulin gradually. The hydrogel with increased crosslinking density could slow down the diffusion speed of insulin inside, resulting in controlled release of insulin and simulating physiological basal insulin secretion. This hydrogel-micelle composite insulin delivery system could quickly reduce the blood glucose level in a mouse model of type 1 diabetes, and maintain normal blood glucose level without hypoglycemia for about 24 h. This kind of glucose-responsive hydrogel-micelle composite may be a promising candidate for delivery of insulin in the treatment of diabetes. | Juan Lv Gang Wu Ying Liu Chang Li Fan Huang Yumin Zhang Jinjian Liu Yingli An Rujiang Ma Linqi Shi | 2019 | Science China Chemistry2019，62，5： | 5 |
| 5 | Optic and catalytic properties of gold nanoparticles tuned by homopolymers显示文摘Gold nanoparticles (GNs) are prepared through in situ reduction using NaBH4 in the presence of homopolymer PDMAEMA. The sizes of the GNs can be adjusted by alternating the molar ratio of gold to DMAEMA. Pure PDMAEMA aqueous solution shows a phase-transition at 50℃ at pH 10 and 25℃ at pH 14, while PDMAEMA-supported GNs aqueous solution shows a phase-transition at 47℃ at pH 10 because of the increasing hydrophobic property resulting from GNs. Due to the pH and tempera-ture-responsible characteristics of PDMAEMA, the resulting PDMAEMA-supported GNs exhibit pH adjustable temperature-responsive characteristics in optic and catalytic aspects. Under an acidic condi-tion (pH 2), the GNs show unchanged surface Plasmon absorbance with a peak of 518 nm in a tem-perature range from 20 to 65℃. Under a basic condition (pH 10), the GNs first show the same absorb-ance with a peak at 518 nm in a temperature range from 20 to 40℃, and then the absorbance red shifts from 518 to 545 nm as temperature increases from 40 to 65℃. When the GNs are used as catalysts to catalyze the reduction of p-nitrophenol, the catalytic activity can be adjusted by changing the permeation of reactants in the PDMAEMA layer at low and high temperatures, respectively. | CHEN Xi ZHAO DongYun ZHAO LiZhi AN YingLi MA RuJiang SHI LinQi HE QiJun CHEN Li | 2009 | Science China Chemistry2009，52，9： | 3 |
| 6 | Biomedical polymers: synthesis, properties, and applications显示文摘Biomedical polymers have been extensively developed for promising applications in a lot of biomedical fields, such as therapeutic medicine delivery, disease detection and diagnosis, biosensing, regenerative medicine, and disease treatment. In this review, we summarize the most recent advances in the synthesis and application of biomedical polymers, and discuss the comprehensive understanding of their property-function relationship for corresponding biomedical applications. In particular, a few burgeoning bioactive polymers, such as peptide/biomembrane/microorganism/cell-based biomedical polymers, are also introduced and highlighted as the emerging biomaterials for cancer precision therapy. Furthermore, the foreseeable challenges and outlook of the development of more efficient, healthier and safer biomedical polymers are discussed. We wish this systemic and comprehensive review on highlighting frontier progress of biomedical polymers could inspire and promote new breakthrough in fundamental research and clinical translation. | Wei-Hai Chen Qi-Wen Chen Qian Chen Chunyan Cui Shun Duan Yongyuan Kang Yang Liu Yun Liu Wali Muhammad Shiqun Shao Chengqiang Tang Jinqiang Wang Lei Wang Meng-Hua Xiong Lichen Yin Kuo Zhang Zhanzhan Zhang Xu Zhen Jun Feng Changyou Gao Zhen Gu Chaoliang He Jian Ji Xiqun Jiang Wenguang Liu Zhuang Liu Huisheng Peng Youqing Shen Linqi Shi Xuemei Sun Hao Wang Jun Wang Haihua Xiao Fu-Jian Xu Zhiyuan Zhong Xian-Zheng Zhang Xuesi Chen | 2022 | Science China Chemistry2022，65，6： | 2 |
| 7 | Thermoresponsive micellization of poly ( ethylene glycol ) bpoly ( nisopropylacrylamide ) in water 显示文摘 | ZHANG Wangqing SHI Linqi WU Kai | 2005 | Macromolecules2005，38，57： | 1 |
| 8 | The effect of cooling conditions on convective heat transfer and flow in a steam-cooled ribbed duct显示文摘 | SHUI Linqi GAO Jianming SHI Xiaojun | 2014 | Journal of Mechanical Science and Technology2014，28，1： | 1 |
| 9 | Formation of spindlelike aggregates and flowerlike arrays of polystyrene-b-poly (acrylic acid) mieelles显示文摘 | Lichao Gao Linqi Shi Yingli An | 2004 | Langmuir2004，20，47： | 1 |
| 10 | Formation of flower-like aggregates from assembly of single polystyrene-b-poly (acrylic acid) rnicelles显示文摘 | Linqi Shi Wangqing Zhang Fenfang Yin | 2004 | New J Chem2004，28，： | 1 |
| 11 | Electric-field-assisted assembly and alignment of polystyrene-Dimly(acrylic acid) micelles 显示文摘 | Guiying Li Linqi Shi Qing Ye | 2006 | Colloid Polym Sci2006，284，： | 1 |
| 12 | Calixarene-integrated nano-drug delivery system for tumortargeted delivery and tracking of anti-cancer drugsin vivo显示文摘Nano-drug delivery systems(nanoDDS)have been extensively investigated clinically to improve the therapeutic effect of anticancer drugs.However,the complicated synthesis during the preparation as well as the potential drug leakage during transportation has greatly limited their general application.In this work,a calixarene-integrated nanoDDS(CanD)that achieves tumor-targeted delivery and tracking of anti-cancer drugs in vivo is presented.The hypoxia-responsive calixarene(SAC4A)exhibits high binding affinity to a series of anti-cancer drugs and rhodamine B(RhB)under normoxic condition while decreasing the binding affinity under hypoxic condition,which leads to the drug release and fluorescence recovery simultaneously.Furthermore,the hypoxia-responsiveness of SAC4A conveys CanD with tumor-targeting ability,resulting in the enrichment of the drug in tumors and enhancement in tumor suppression in mice.Moreover,CanD could become a general platform allowing the delivery of a wide scope of anti-cancer drugs that have strong host-guest interaction with SAC4A. | Lina Xu Jingshan Chai Ying Wang Xinzhi Zhao Dong-Sheng Guo Linqi Shi Zhanzhan Zhang Yang Liu | 2022 | Nano Research2022，15，8： | 1 |
| 13 | Formation of complex micelles with double-responsive channels from self-assembly of two diblock copolymers 显示文摘 | Li Guiying Shi Linqi Ma Rujiang | 2006 | Angewandte Chemic-International Edition2006，45，30： | 1 |
| 14 | Double-responsive core-shell-corona mieelles from self-assembly of diblock eopolymer of poly(t-butyl acrylate-co-acrylic)-b-poly(N-isopropylacrylamide)显示文摘 | Li Guiying Shi Linqi An Yingli | 2006 | Polymer2006，47，13： | 1 |
| 15 | Thermoresponsive micellization of poly ( ethylene glycol)-b-poly ( N-isopropylacrylamide) in water显示文摘 | Zhang Wangqing Shi Linqi Wu Kai | 2005 | Macromolecules2005，38，13： | 1 |
| 16 | Reverse micelles of star - block copolymer as nanoreactors for preparation of gold nanoparticles显示文摘 | Li Junbo Shi Linqi An Yingli | 2006 | Polymer2006，47，： | 1 |
| 17 | Evaporation-induced aggregation of polystyrene-block-poly(acrylic acid) micelles to microcubic particles显示文摘 | Zhang Wangqing Shi Linqi An Yingli | 2003 | Langmuir2003，19，15： | 1 |
| 18 | Reverse micelles of star-block copolymer as nanoreactors for preparation of gold nanoparticles显示文摘 | Li Junbo Shi Linqi An Yingli | 2006 | Polymer2006，478，： | 1 |
| 19 | Persistence of VRC01-resistant HIV-1 during antiretroviral therapy显示文摘VRC01,a broadly neutralizing monoclonal antibody(bnmAb),can neutralize a diverse array of HIV-1 isolates by mimicking CD4 binding to the envelope glycoprotein gp120.We have previously demonstrated the presence of VRC01-resistant strains in an HIV-1 infected patient during antiretroviral therapy.Here,we report follow-up studies of two subsequent samples from the same patient.With genetic and phenotypic analysis of over 70 full-length molecular clones of the HIV-1 envelope,we show that VRC01-resistant HIV-1 continued to exist and change in its proportion of the infecting virus during treatment with a highly active antiretroviral therapy.Consistent with our previous observation,the resistant phenotype was associated with a single asparagine residue at position 460(N460),a potential N-linked glycosylation site in the V5 region.The persistence and continuing evolution of VRC01-resistant HIV-1 in vivo presents a great challenge to our future preventative and therapeutic interventions based on VRC01. | GUO DongXing SHI XuanLing SONG DingKa ZHANG LinQi | 2014 | Science China(Life Sciences)2014，57，1： | 1 |
| 20 | Recent advances and future challenges in the use of nanoparticles for the dispersal of infectious biofilms显示文摘Increasing occurrence of intrinsically antimicrobial-resistant,human pathogens and the protective biofilm-mode in which they grow,dictates a need for the alternative control of infectious biofilms.Biofilm bacteria utilize dispersal mechanisms to detach parts of a biofilm as part of the biofilm life-cycle during times of nutrient scarcity or overpopulation.We here identify recent advances and future challenges in the development of dispersants as a new infection-control strategy.Deoxyribonuclease(DNase)and other extracellular enzymes can disrupt the extracellular matrix of a biofilm to cause dispersal.Also,a variety of small molecules,reactive oxygen species,nitric oxide releasing compounds,peptides and molecules regulating signaling pathways in biofilms have been described as dispersants.On their own,dispersants do not inhibit bacterial growth or kill bacterial pathogens.Both natural,as well as artificial dispersants,are unstable and hydrophobic which necessitate their encapsulation in smart nanocarriers,like p H-responsive micelles,liposomes or hydrogels.Depending on their composition,nanoparticles can also possess intrinsic dispersant properties.Bacteria dispersed from an infectious biofilm end up in the blood circulation where they are cleared by host immune cells.However,this sudden increase in bacterial concentration can also cause sepsis.Simultaneous antibiotic loading of nanoparticles with dispersant properties or combined administration of dispersants and antibiotics can counter this threat.Importantly,biofilm remaining after dispersant administration appears more susceptible to existing antibiotics.Being part of the natural biofilm life-cycle,no signs of'dispersant-resistance'have been observed.Dispersants are therewith promising for the control of infectious biofilms. | Shuang Tian Henny C.van der Mei Yijin Ren Henk J.Busscher Linqi Shi | 2021 | Journal of Materials Science & Technology2021，，25： | 1 |
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