|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Does combined therapy of curcumin and epigallocatechin gallate have a synergistic neuroprotective effect against spinal cord injury?显示文摘Systematic inflammatory response after spinal cord injury(SCI) is one of the factors leading to lesion development and a profound degree of functional loss. Anti-inflammatory compounds, such as curcumin and epigallocatechin gallate(EGCG) are known for their neuroprotective effects. In this study, we investigated the effect of combined therapy of curcumin and EGCG in a rat model of acute SCI induced by balloon compression. Immediately after SCI, rats received curcumin, EGCG, curcumin + EGCG or saline [daily intraperitoneal doses(curcumin, 6 mg/kg; EGCG 17 mg/kg)] and weekly intramuscular doses(curcumin, 60 mg/kg; EGCG 17 mg/kg)] for 28 days. Rats were evaluated using behavioral tests(the Basso, Beattie, and Bresnahan(BBB) open-field locomotor test, flat beam test). Spinal cord tissue was analyzed using histological methods(Luxol Blue-cresyl violet staining) and immunohistochemistry(anti-glial fibrillary acidic protein, anti-growth associated protein 43). Cytokine levels(interleukin-1β, interleukin-4, interleukin-2, interleukin-6, macrophage inflammatory protein 1-alpha, and RANTES) were measured using Luminex assay. Quantitative polymerase chain reaction was performed to determine the relative expression of genes(Sort1, Fgf2, Irf5, Mrc1, Olig2, Casp3, Gap43, Gfap, Vegf, NfκB, Cntf) related to regenerative processes in injured spinal cord. We found that all treatments displayed significant behavioral recovery, with no obvious synergistic effect after combined therapy of curcumin and ECGC. Curcumin and EGCG alone or in combination increased axonal sprouting, decreased glial scar formation, and altered the levels of macrophage inflammatory protein 1-alpha, interleukin-1β, interleukin-4 and interleukin-6 cytokines. These results imply that although the expected synergistic response of this combined therapy was less obvious, aspects of tissue regeneration and immune responses in severe SCI were evident. | Jiri Ruzicka Lucia Machova Urdzikova Barbora Svobodova Anubhav G. Amin Kristyna Karova Jana Dubisova Kristyna Zaviskova Sarka Kubinova Meic Schmidt Meena Jhanwar-Uniyal Pavla Jendelova | 2018 | Neural Regeneration Research2018，13，1： | 4 |
| 2 | Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression显示文摘Background: SIRT1 histone deacetylase acts on many epigenetic and non-epigenetic targets. It is thought that SIRT1 is involved in oocyte maturation;therefore, the importance of the ooplasmic SIRT1 pool for the further fate of mature oocytes has been strongly suggested. We hypothesised that SIRT1 plays the role of a signalling molecule in mature oocytes through selected epigenetic and non-epigenetic regulation.Results: We observed SIRT1 re-localisation in mature oocytes and its association with spindle microtubules.In mature oocytes, SIRT1 distribution shows a spindle-like pattern, and spindle-specific SIRT1 action decreasesα-tubulin acetylation. Based on the observation of the histone code in immature and mature oocytes, we suggest that SIRT1 is mostly predestined for an epigenetic mode of action in the germinal vesicles(GVs) of immature oocytes. Accordingly, BML-278-driven trimethylation of lysine K9 in histone H3 in mature oocytes is considered to be a result of GV epigenetic transformation.Conclusions: Taken together, our observations point out the dual spatiotemporal SIRT1 action in oocytes,which can be readily switched from the epigenetic to non-epigenetic mode of action depending on the progress of meiosis. | Jan Nevoral Lukas Landsmann Miriam Stiavnicka Petr Hosek Jiri Moravec Sarka Prokesova Hedvika Rimnacova Eliska Koutna Pavel Klein Kristyna Hoskova Tereza Zalmanova Tereza Fenclova Jaroslav Petr Milena Kralickova | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 3 |
| 3 | Antibodies Against Annexin V and Prothrombin,Their Correlation with Other Anti-phospholipid Antibodies in Recurrent Pregnancy Loss显示文摘Objective To study the findings of serum antibodies against annexin V, prothrombin,ph-inositol, ph-acid, ph-ethanolamine, ph-serine, ph-glycerol, cardiolipin, and beta2-glycoprotein I and analyze the trophoblast annexin V receptorsMethods Sera from 156 patients aged 26-41 years with recurrent pregnancy loss (3-7 times) were investigated. Eighty-four fertile healthy women aged 24-38 yearswere included in a control group. ELISA methods were used for detecting a panel of seraanti-phospholipid antibodies. Immunolocalization of annexin V receptors in 143trophoblast specimens of 156 patients was investigated by the immunofluorescencetechnique using Annexin V-FITC, Apoptosis and AnnexinV-CY3 commercial kits.Results Positivity for anti-phospholipid antibodies mainly against ph-serine, ph-ethanolamine, and ph-inositol was found together in 80.8% (126 out of 156 patients),anti-prothrombin antibodies in 12% (18), and anti-annexin V antibodies in 13. 5%(21) women. No significant levels of anti-phospholipid antibodies were found in 6controls. Placenta immunohistopathology also exhibited some changes manifested bythe presence of apoptotic and necrotic cells in trophoblast, and very fewmicrothrombotization in some intervillous spaces.Conclusion Our detailed study demonstrated the prevalence of majority of anti-phospholipid antibodies as a high risk factor for repeated reproductive failure. Verylow microthrombosis in placentas could be explained by the changes ofhaemocoagulation properties out of uterus. | Zdenka Ulcova-Gallova Petr Mukensnabl Sarka Hadravska Vendula Kyselova Jitka Slechtova Zdenka Micanova Katerina Bibkova Miroslav Balvin Zdenek Rokyta | 2005 | Journal of Reproduction and Contraception2005，16，1： | 3 |
| 4 | Stages of oocyte development in the zebrafish, Brachydanio Rerio 显示文摘 | Selman K Wallace R A Sarka A | 1993 | Journal of Morphol1993，218，： | 1 |
| 5 | Structure of biologically active muramyl peptides from peptidoglycan of Streptococcum Sanguis显示文摘 | SARKA B DOMINIC M D MICHAEL J P | 1998 | J Mass Spectrom1998，33，： | 1 |
| 6 | Process Capabilities and Value Generation in Alliance Portfolios显示文摘 | SARKA R AULAKH MADHOK | 2009 | Organization Scince2009，20，3： | 1 |
| 7 | Phthalate and PAH concentrations in dust collected from Danish homes and daycare centers 显示文摘 | Sarka Langer Charles J Weschler Andreas Fischer | 2010 | Atmospheric Environment2010，44，19： | 1 |
| 8 | Learning from Supply-Side Agents:The Impact of Technology Solution Providers' Experiential Diversity on Clients' Innovation Adoption 显示文摘 | Weigelt C Sarka M | 2009 | Academy of Management Journal2009，52，1： | 1 |
| 9 | Correlations and regressions among oil content,grain yield and yield components in maize显示文摘 | RAMAN R SARKA K R | 1983 | Indian Agric Sci1983，53，5： | 1 |
| 10 | Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses 显示文摘 | Jakub Sire Sarka Kubinova Radka Hobzova | 2012 | International Journal of Nanomedicine2012，，7： | 1 |
| 11 | Stages of oocyte develo- pment in the zebrafish, Brachydanio rerio显示文摘 | SELMAN K WALLACE R A SARKA A | 1993 | Joamal of Morphology1993，218，： | 1 |
| 12 | Understanding the language of vitamin C 显示文摘 | Sarka ' N Srivastava P K Dubey V K | 2009 | Current Nutrition & Food Science2009，5，1： | 1 |
| 13 | Specific cytoskeleton changes during apoptosis accompanying induced differenti- ation of HL-60 myeloid leukemia cells 显示文摘 | Renata V Karel Z Sarka J | 2003 | Oncol Rep2003，14，10： | 1 |
| 14 | Comparative physiological and molecular responses of a common aromatic indica rice cultivar to high salinity with non-aromatic indic rice cultivars显示文摘 | ROYCHOUDHURY A S BASU S N SARKA R | | 0，，： | 1 |
| 15 | An effieienl differential box-count- ing approach to compute fractal dimension of image 显示文摘 | SARKA N CHAUDHURI B B | 1994 | IEEE Transactions on Systems Man and Cybernetics1994，24，1： | 1 |
| 16 | Extracellular matrix based biomaterials for central nervous system tissue repair: the benefits and drawbacks显示文摘Functional repair of injured tissue in the adult central nervous system(CNS)still remains a big challenge for current biomedical research and its upcoming clinical translation.The axonal regeneration of the adult CNS is generally low,and it is additionally restricted after injury by the presence of inhibitory molecules,generated by the glial scar.Current preclinical treatment strategies after CNS injury include cell-based therapies,gene therapy and drug and | Sarka Kubinova | 2017 | Neural Regeneration Research2017，12，9： | 1 |
| 17 | MR spectroscopy quantitation:a review of frequency-domain methods显示文摘 | Mika Ala-Korpela | 2001 | NMR Biomed2001，14，1： | 1 |
| 18 | Potential of acidophilus milk to lower cholesterol显示文摘 | SARKA S | 2003 | Nutrition and Food Science2003，，33： | 1 |
| 19 | Prediabetes/early diabetes‐associated neuropathy predominantly involves sensory small fibres显示文摘 | Sarka Divisova Eva Vlckova Maria Hnojcikova Miroslav Skorna Martin Nemec Petr Dubovy Ladislav Dusek Jiri Jarkovsky Jana Belobradkova Josef Bednarik | 2012 | Journal of the Peripheral Nervous System2012，，3： | 1 |
| 20 | Possibilities forthe ues of membrane processes for the pre-treatment ofwastewater from the production of dried potato pur e e显示文摘 | SARKA E POUR V VESELA A | 2009 | Desalination2009，249，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员