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| 1 | An overview of animal models for investigating the pathogenesis and therapeutic strategies in acute hepatic failure显示文摘Acute hepatic failure (AHF) is a severe liver injury accompanied by hepatic encephalopathy which causes multiorgan failure with an extremely high mortality rate, even if intensive care is provided. Management of severe AHF continues to be one of the most challenging problems in clinical medicine. Liver transplantation has been shown to be the most effective therapy, but the procedure is limited by shortage of donor organs. Although a number of clinical trials testing different liver assist devices are under way, these systems alone have no significant effect on patient survival and are only regarded as a useful approach to bridge patients with AHF to liver transplantation. As a result, reproducible experimental animal models resembling the clinical conditions are still needed. The three main approaches used to create an animal model for AHF are: surgical procedures, toxic liver injury and infective procedures. Most common models are based on surgical techniques (total/partial hepatectomy, complete/transient devascularization) or the use of hepatotoxic drugs (acetaminophen, galactosamine, thioacetamide, and others), and very few satisfactory viral models are available. We have recently developed a viral model of AHF by means of the inoculation of rabbits with the virus of rabbit hemorrhagic disease. This model displays biochemical and histological characteristics, and clinical features that resemble those in human AHF. In the present article an overview is given of the most widely used animal models of AHF, and their main advantages and disadvantages are reviewed. | María Jesús Tuón Marcelino Alvarez Jesús M Culebras Javier González-Gallego | 2009 | World Journal of Gastroenterology2009，15，25： | 29 |
| 2 | 非常规油气开发中润湿性反转技术的应用显示文摘研究了适用于非常规油气储集层的岩石润湿性测量方法,总结了非常规油气储集层岩石润湿性特征,分析了非常规油气储集层岩石润湿性反转技术的应用情况,并介绍了研究表面活性剂与非常规油气储集层岩石润湿性间关系的实验方法。研究表明:接触角法、核磁共振法和Zeta电位测量法是评估非常规油气储集层岩石润湿性最恰当的方法,非常规油气储集层呈现出中性润湿到油湿的混合润湿特性。润湿性反转技术在非常规油气储集层中的应用目前仅限于添加表面活性剂。在压裂液中加入表面活性剂可改变非常规油气储集层岩石润湿性从而提高原油采收率。借助稳定性实验、润湿性反转评估实验、界面张力实验、自吸实验、压吸实验和吸附实验等可评估表面活性剂改变润湿性及提高采收率的效率。 | ALVAREZ J O SCHECHTER D S | 2016 | 石油勘探与开发2016，43，5： | 15 |
| 3 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 4 | AMP-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the reRulation of sperm motility显示文摘 | Violeta Calle-Guisado Ana Hurtado de Llera David Martin-Hidalgo Jose Mijares Maria C Gil Ignacio S Alvarez Maria J Bragado Luis J Garcia-Marin | 2017 | Asian Journal of Andrology2017，19，6： | 5 |
| 5 | Intraoperative Management and Repair of Bile Duct Injuries Sustained during 10,123 Laparoscopic Cholecystectomies in a High-Volume Referral Center显示文摘 | Juan Pekolj Fernando A. Alvarez Martin Palavecino Rodrigo Sánchez Clariá Oscar Mazza Eduardo de Santiba?es | 2013 | Journal of the American College of Surgeons2013，，5： | 3 |
| 6 | Transabdominal Surgical Approach in the Management of Renal Tumors Involving the Retrohepatic Inferior Vena Cava显示文摘 | Jose A. Gonzalez-Fajardo Ernesto Fernandez Jesús Rivera Alejandro Pelaz Javier Gonzalez-Zarate Juan C. Alvarez E. González C. Vaquero | 2000 | Annals of Vascular Surgery2000，，5： | 2 |
| 7 | Nutrient and heavy metal concentration and distribution in corn, soybean and wheat as affected by different tillage systems in the Argentine Pampas显示文摘 | Raúl S Lavado Claudia A Porcelli Roberto Alvarez | 2001 | Soil & Tillage Research2001，，1： | 2 |
| 8 | Ileocolic intussusception due to intestinal metastatic melanoma. Case report and review of the literature显示文摘 | Fernando A. Alvarez Matías Nicolás Jeremías Goransky Carlos A. Vaccaro Axel Beskow Demetrio Cavadas | 2011 | International Journal of Surgery Case Reports2011，，6： | 2 |
| 9 | RNA-dependent RNA polymerase gene analysis of worldwide Newcastle disease virus isolates representing different virulence types and their phylogenetic relationship with other members of the paramyxoviridae 显示文摘 | Wise M G Sellers H S Alvarez R | 2004 | Virus Res2004，104，1： | 1 |
| 10 | Habit Formation, Catching up with the Joneses, and Economic Growth显示文摘 | Alvarez F G Monteiro S J Tttmovsky | 2004 | Journal of Economic Growth2004，，9： | 1 |
| 11 | Quadratic-based computation of four-impulse optimal rendezvous near circular orbit 显示文摘 | Carter T E Alvarez S A | 2000 | Journal of Guidance Control and Dynamics2000，23，1： | 1 |
| 12 | How do Entrepreneurs organize firms under conditions of uncertainty 显示文摘 | Alvarez S A Barney J B | 2005 | Journal of management2005，31，5： | 1 |
| 13 | Addition of ultralow dose naloxone to postoperative morphine PCA:unchanged analgesia and opioid requirement but decreased incidence of opioid side effects显示文摘 | Cepeda M S Alvarez H Morales 0 | 2004 | Pain2004，107，12： | 1 |
| 14 | Prevention of gastrointestinal infection using immunobiological methods with milk fermented with Lactobacillus casei and Lactobacillus acidophillus显示文摘 | Perdigon G Macias M Alvarez S | 1990 | J Dairy Res1990，57，2： | 1 |
| 15 | Acetaldehyde increases the activity and gene expression of urokinase type plasminogen activator in a hepatic stellate cell line显示文摘 | Rodrīguez-Fragoso L Alvarez R Reyes-Esparza JA Garcés ME | 1999 | Toxicology1999，137，： | 1 |
| 16 | Synthesis of polyaniline using horseradish peroxidase immobilized on plasma-functionalized polyethylene surfaces as initiator显示文摘 | Alvarez S Manolache S Denes F | 2003 | Journal of Applied Polymer Science2003，88，2： | 1 |
| 17 | Stereochemistry o{ compounds with coordination number ten显示文摘 | Ruiz-Martinez A Alvarez S | 2009 | Chem-Eur2009，15，30： | 1 |
| 18 | Citalopram,escitalopram and prolonged QT:Warning or alarm?显示文摘 | Alvarez E Vieira S Garcia-Moll X | 2014 | Rev Psiquiatr Salud Ment2014，7，3： | 1 |
| 19 | Changes in polyphenol and polysaecharide content of grape seed extract and grape pomace after enzymatic treatment 显示文摘 | CHAMORRO S VIVEROS A ALVAREZ I | 2012 | Food Chemistry2012，133，2： | 1 |
| 20 | Determination of organic acids in grape musts, wines and vinegars by high performance liquid-chromatography显示文摘 | ROMERO E G MUNOZ G S ALVAREZ P J M | 1993 | Journal of Chromatography A1993，655，1： | 1 |
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