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| 1 | Effects of Biochar and Super Absorbent Polymer on Substrate Properties and Water Spinach Growth显示文摘Most previous researches have focused on biochar application in agricultural soils; however, limited information is available concerning the effects of biochar amendment on greenhouse substrate properties. A greenhouse experiment was conducted to investigate effects of wheat straw biochar(0–160 m L L-1) and super absorbent polymer(SAP, 0.8 g L-1) on physical and chemical properties of a substrate based on spent pig litter compost and the growth of water spinach(Ipomoea aquatica Forsk). Total porosity, water-holding capacity, p H and electrical conductivity(EC) of the substrate significantly increased with increasing biochar rates, especially in the substrate without SAP. The values of p H and EC were significantly lower in the substrate with SAP than those without SAP at the high biochar application rates(100–160 m L L-1). The germination rates of water spinach decreased with increasing biochar rates when biochar was added alone(76.9%–83.7%), whereas the rates increased to 83.6%–85.8% when biochar was added in combination with SAP. Growth parameters of water spinach and nutrient uptake by shoots and roots increased with increasing biochar rates and reached the maximum values at the biochar rate of 100 m L L-1. There were significant cubic relationships between the uptake of nutrients(N, P, and K) and biochar rates, both with and without SAP addition. In order to avoid negative effects on plant growth,the biochar application rate should be controlled at an optimal level(100 m L L-1). The SAP addition not only enhanced the positive effects of biochar application on the properties of the substrate, but also inhibited the excessive rise of p H and EC following biochar additions, which led to better plant growth and enhanced nutrient uptakes by water spinach. | FAN Ruqin LUO Jia YAN Shaohua ZHOU Yunlai ZHANG Zhenhua | 2015 | Pedosphere2015，25，5： | 15 |
| 2 | Long Distance Transmission of SARS-CoV-2 from Contaminated Cold Chain Products to Humans——Qingdao City,Shandong Province,China,September 2020显示文摘Summary What is already known about this topic?Though coronavirus disease 2019(COVID-19)has largely been controlled in China,several outbreaks of COVID-19 have occurred from importation of cases or of suspected virus-contaminated products.Though several outbreaks have been traced to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)isolated on the outer packaging of cold chain products,live virus has not been obtained. | Huilai Ma Zhaoguo Wang Xiang Zhao Jun Han Yong Zhang Hong Wang Cao Chen Ji Wang Fachun Jiang Jie Lei Jingdong Song Shaofeng Jiang Shuangli Zhu Huihui Liu Dongyan Wang Yao Meng Naiying Mao Yanhai Wang Zhen Zhu Zhixiao Chen Bingling Wang Qinqin Song Haijun Du Qun Yuan Dong Xia Zhiqiang Xia Peipei Liu Yuchao Wu Zijian Feng Ruqin Gao George F.Gao Wenbo Xu | 2021 | China CDC weekly2021，3，30： | 13 |
| 3 | A Nosocomial COVID-19 Outbreak Initiated by an Infected Dockworker at Qingdao City Port-Shandong Province, China, October, 2020显示文摘On October 10,2020,3 cases of coronavirus disease 2019(COVID-19)were detected via routine preadmission nucleic acid screening of inpatients and their visitors at Qingdao Central Hospital.The 3 cases were all related to the Qingdao Chest Hospital.An additional 9 cases were discovered and confirmed through tracing and testing contacts of the 3 initial cases.Viral isolates from all 12 cases were found to have high degrees of genetic homology with the viral isolate from a dockworker at Qingdao Port,referred to as Patient A in this report.A joint investigation team was formed to determine the source of infection and the scale of the outbreak,and to prevent further spread of the virus. | Qun Yuan Zengqiang Kou Fachun Jiang Zhongjie Li Lijie Zhang Huihui Liu Xiang Zhao Dianmin Kang Ruqin Gao Jie Lei | 2020 | China CDC weekly2020，2，43： | 12 |
| 4 | Effects of Tillage Management on Infiltration and Preferential Flow in a Black Soil, Northeast China显示文摘The impacts of no-tillage (NT) and moldboard plough (MP) managements on infiltration rate and preferential flow were characterized using a combined technique of double-ring device and dye tracer on a black soil (Mollisols) in Northeast China. The objective of this study is to evaluate how tillage practices enhance soil water infiltration and preferential flow in favor of soil erosion control in the study area. The steady infiltration rates under NT management are 1.6 and 2.1 times as high as those under MP management in the 6th and 8th years of the tillage management in place, while the infiltrated water amounts under NT management are 1.4 and 2.0 times as high as those under MP management, respectively. The depth of methylene blue penetrated into NT soil increases from 43cm in the 6th year to 57cm in the 8th year, which are 16cm and 19cm deeper than those in MP soil, respectively. The results of morphologic image show that more biological macro-pores occur in NT soil than in MP soil. These macro-pores play a key role in enhancing preferential flow in NT soil, which in turn promotes water infiltration through preferential pathways in NT soil. The results are helpful to policy-making in popularizing NT and have the implications for tillage management in regard to soil erosion control in black soil region of China. | FAN Ruqin ZHANG Xiaoping YANG Xueming LIANG Aizhen JIA Shuxia CHEN Xuewen | 2013 | Chinese Geographical Science2013，23，3： | 6 |
| 5 | Homogeneous label-free fluorescent assay of small molecule-protein interactions using protein binding-inhibited transcription nanomachine显示文摘Quantitative analysis of interactions between small molecules and proteins is a central challenge in chemical genetics, molecular diagnostics and drug developments. Here, we developed a RNA transcription nanomachine by assembling T7 RNA polymerase on a small molecule-labeled DNA heteroduplex. The nanomachine, of which the RNA transcription activity can be quantitatively inhibited by protein binding, showed a great potential for small molecule-protein interaction assay. This finding enabled us to develop a novel homogeneous label-free strategy for assays of interactions between small molecules and their protein receptors. Three small molecule compounds and their protein receptors have been used to demonstrate the developed strategy. The results revealed that the protein-small molecule interaction assay strategy shows dynamic responses in the concentration range from 0.5 to 64 nM with a detection limit of 0.2 nM. Due to its label-free, homogeneous, and fluorescence-based detection format, besides its desirable sensitivity this technique could be greatly robust, cost-efficient and readily automated, implying that the developed small molecule-protein interaction assay strategy might create a new methodology for developing intrinsically robust, sensitive and selective platforms for homogeneous protein detection. | ZHOU DianMing, WU YiDan, LIU Pei, BAI HaoTian, TANG LiJuan, YU RuQin & JIANG JianHui State Key Laboratory of Chemo/Bio-Sensing and Chemometrics College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China | 2011 | Science China Chemistry2011，54，8： | 4 |
| 6 | HPLC-DAD data coupled with second-order calibration method applied to food analysis: Simultaneous determination of six benzoylurea insecticides in various fruit samples by selecting time region of chromatogram显示文摘This work presents a novel application of second-order calibration based on self-weighted alternating trilinear decomposition(SWATLD)algorithm for analyzing the HPLC-DAD data.The proposed method makes it possible to simultaneously determine teflubenzuron,hexaflumuron,flufenoxuron,chlorfluazuron,diflubenzuron and benzoylurea in different fruit samples,i.e.pear,apple and banana,in the selected time region of chromatogram.The concentration,elution time and spectral information of these benzoylurea insecticides are selectively extracted from complex matrices even in the presence of unknown interferences.The root-mean-square error of prediction(RMSEP)and figures of merit,including sensitivity(SEN),selectivity(SEL)and limit of detection(LOD)are employed to access the performance of the method.The LODs obtained for these insecticides are within the range 0.017–0.26 ppm in pears,0.039–0.33 ppm in apples,0.041–0.44 ppm in bananas,respectively.Such a chemometrics-based protocol holds great potential to be extended as a promising alternative for more practical applications in food safety and quality monitoring. | QING XiangDong WU HaiLong NIE ChongChong LI Yong YAN XiuFang ZHANG XiHua YIN XiaoLi YU RuQin | 2013 | Science China Chemistry2013，56，11： | 2 |
| 7 | Rapid determination of thiabendazole in orange extract using excitation-emission matrix fluorescence and second-order calibration based on alternating trilinear decomposition/alternating normalization-weighted error algorithms显示文摘A novel approach is proposed for direct quantitative analysis of thiabendazole in the orange extract by using excitation-emission matrix fluorescence coupled with second-order calibration methods based on the alternating trilinear decomposition(ATLD) and the alternating normalization-weighted error(ANWE) algorithms,respectively. The average recoveries of thiabendazole in the orange extract by using ATLD and ANWE with an estimated component number of two were 99.7 ± 3.3% and 103.5 ± 4.1%,respectively. Furthermore,the accuracy of the two algorithms was also evaluated through elliptical joint confidence region(EJCR) tests as well as figures of merit,such as sensitivity(SEN),selectivity(SEL) and limit of detection(LOD). The experimental results demonstrate that both algorithms have been satisfactorily applied to the determination of thiabendazole in orange extract,and the perform-ance of ANWE is slightly better than that of ATLD. | WANG XueMei WU HaiLong NIE JinFang LI YuanNa YU YongJie YU RuQin | 2008 | Science China Chemistry2008，51，8： | 2 |
| 8 | Interaction of epicatechin with bovine serum albumin using fluorescence quenching combined with chemometrics显示文摘The interaction between BSA and epicatechin was studied using fluorescence quenching titrations combined with trilinear decomposition method and excitation-emission matrix(EEM)fluorescence.The resolved spectra were highly similar with the actual ones which indicated that the resolved results were reliable.The relevant parameters of the binding process were obtained by quantifying each substance in the complicated mixtures in situ.The quenching was static quenching,epicatechin had a weak interaction with BSA and the binding site was one.The total concentration and the free concentration of quenchers had different effect on the system.The results demonstrated that the method exploited in this article is a useful tool to investigate complicated interactions,avoiding complicated pretreatment and simplify experimental procedure. | ZHAI Min WU HaiLong ZHANG ShuRong ZHANG XiHua SUN YanMei YU RuQin | 2014 | Science China Chemistry2014，57，5： | 2 |
| 9 | Evaluation of Image-Analysis-Based Drape Tester显示文摘The performance of image-analysis-based drape tester is studied by analyzing the testing results. The accelerating and decelerating method is used to make the initial testing condition same before draping. A feature vector is proposed to describe the drapenbility of fabrics comprehensively. Moreover, the exponential relationship curves between drape coefficient and rotating speed are obtained from the drape tester to describe the dynamic drapeability Finally, the stability of tested results of image-analysis-based system is studied. | GuoYongping(郭永平) Li Ruqin(李汝勤) | 2001 | Journal of Donghua University(English Edition)2001，18，2： | 1 |
| 10 | Microwave plasma treated carbon nanotubes and their electrochemical biosensing application显示文摘 | Zhaoyang Wu Yinyu Xu Xiaolei Zhang Guoli Shen Ruqin Yu | 2007 | Talanta2007，，4： | 1 |
| 11 | Similar distribution of simple sequence repeats in diverse completed Human lmmunodeficiency Virus Type 1 genomes显示文摘 | Ming Chen Zhongyang Tan Jianhui Jiang Mingfu Li Hongjun Chen and Ruqin Yu | 2009 | Federation of European Biochemical Societies2009，583，： | 1 |
| 12 | Amperometric glucose biosensor based on chitosan with improved selectivity and stability显示文摘 | Minghui Yang Yunhui Yang Bing Liu Guoli Shen Ruqin Yu | 2004 | Sensors & Actuators: B Chemical2004，，3： | 1 |
| 13 | Prediction of the Q-e parameters from transition state structures 显示文摘 | Xinliang Yu Ruqin Yu | 2013 | Polymer Engineering & Science2013，53，10： | 1 |
| 14 | Preparation and photo-catalytic activity of supported TiO2 composites显示文摘 | GAO Ruqin ZHU Lingfeng | 2010 | IEEE2010，，10： | 1 |
| 15 | A Reusable Piezo-Immunosensor with Amplified Sensitivity for Ceruloplasmin Based on Plasma-Polymerized Film显示文摘 | Hua Wang Dan Li Zhaoyang Wu Guoli Shen and Ruqin Yu | 2004 | Talanta2004，62，1： | 1 |
| 16 | Contribution of winter soil respiration to annual soil CO2 emission in a Mollisol under different tillage practices in northeast China显示文摘 | Shi Xiuhuan Zhang Xiaoping Yang Xueming Drury Craig F. McLaughlin Neil B. Liang Aizhen Fan Ruqin Jia Shuxia | 2012 | EN2012，，2： | 1 |
| 17 | Preparation and characterization of prussian blue nanowire array and bioapplication for glucose biosensing显示文摘 | QU Fengli SHI Aiwu YU Ruqin | 2007 | Anal Chim Acta2007，605，1： | 1 |
| 18 | Epinephrine regulation of the endothelial nitricoxide synthase roles of racl and β3-adrenergic receptors in endothelial no signaling显示文摘 | Ruqin K Thomas M | 2007 | Biol Chem2007，282，32： | 1 |
| 19 | A Protein A-Based Orientation-Controlled Immobilization Strategy for Antibodies Using Nanometer-Sized Gold Particles and Plasma-Polymerized Film显示文摘 | Hua Wang Yanli Liu Yunhui Yang Ting Deng Guoli Shen and Ruqin Yu | 2004 | Analytical Biochemistry2004，324，2： | 1 |
| 20 | Double strand DNA-templated copper nanoparticle as a novel fluorescence indicator for label-free detection of polynucleotide kinase activity显示文摘 | Liangliang Zhang Jingjin Zhao Hua Zhang Jianhui Jiang Ruqin Yu | 2013 | Biosensors and Bioelectronics2013，，： | 1 |
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