|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Chromosome-scale genome assembly provides insights into the evolution and flavor synthesis of passion fruit (Passiflora edulis Sims)显示文摘Passion fruit(Passiflora edulis Sims)is an economically valuable fruit that is cultivated in tropical and subtropical regions of the world.Here,we report an~1341.7Mb chromosome-scale genome assembly of passion fruit,with 98.91%(~1327.18Mb)of the assembly assigned to nine pseudochromosomes.The genome includes 23,171 protein-coding genes,and most of the assembled sequences are repetitive sequences,with long-terminal repeats(LTRs)being the most abundant.Phylogenetic analysis revealed that passion fruit diverged after Brassicaceae and before Euphorbiaceae.Ks analysis showed that two whole-genome duplication events occurred in passion fruit at 65 MYA and 12 MYA,which may have contributed to its large genome size.An integrated analysis of genomic,transcriptomic,and metabolomic data showed that‘alpha-linolenic acid metabolism’,‘metabolic pathways’,and‘secondary metabolic pathways’were the main pathways involved in the synthesis of important volatile organic compounds(VOCs)in passion fruit,and this analysis identified some candidate genes,including GDP-fucose Transporter 1-like,Tetratricopeptide repeat protein 33,protein NETWORKED 4B isoform X1,and Golgin Subfamily A member 6-like protein 22.In addition,we identified 13 important gene families in fatty acid pathways and eight important gene families in terpene pathways.Gene family analysis showed that the ACX,ADH,ALDH,and HPL gene families,especially ACX13/14/15/20,ADH13/26/33,ALDH1/4/21,and HPL4/6,were the key genes for ester synthesis,while the TPS gene family,especially PeTPS2/3/4/24,was the key gene family for terpene synthesis.This work provides insights into genome evolution and flavor trait biology and offers valuable resources for the improved cultivation of passion fruit. | Zhiqiang Xia Dongmei Huang Shengkui Zhang Wenquan Wang Funing Ma Bin Wu Yi Xu Bingqiang Xu Di Chen Meiling Zou Huanyu Xu Xincheng Zhou Rulin Zhan Shun Song | 2021 | Horticulture Research2021，8，1： | 15 |
| 2 | Anatomical variation of infra-pyloric artery origination: A prospective multicenter observational study (IPA-Origin)显示文摘Objective: Infra-pyloric artery(IPA) is an important anatomical landmark in treatment of gastric cancer and is the key vessel for pylorus-preserving gastrectomy and subgroup of infra-pyloric lymph nodes. However, its anatomical variation is not thoroughly understood. Our study aimed to clarify the origination of the IPA.Methods: We did this prospective, multicenter, open-label, observational study at gastric surgery departments of34 hospitals in China. Gastric cancer patients aged 18 years or older and scheduled to undergo elective total or distal gastrectomy were assigned. During the surgery, IPA dissecting and exposing the origination point with photographs or video clips were required. The primary outcome was the origination of the IPA. Analysis of variance, χ~2 tests and Fisher's tests were used to analyze the differences between groups. The study is registered at Clinicaltrials.gov(No. NCT03071237).Results: Between May 8 and July 31, 2017, 429 patients were assigned for the study, and 419(97.7%) patients had the IPA dissected and recorded through photograph or video and were included in the primary outcome analysis. The median age was 62 years old, and 73.7% were male. Among the patients, 78.5% received laparoscopic surgery. Single IPA origination was identified in 398(95.0%) patients, including gastroduodenal artery(GDA) in154(36.8%) patients, anterior superior pancreaticoduodenal artery(ASPDA) in 130(31.0%) patients, and right gastroepiploic artery(RGEA) in 114(27.2%) patients. Fifteen(3.6%) patients were identified with multiple IPA and 6(1.4%) patients were identified as IPA absence. The differences in the distribution of surgical approach(P=0.003) and geographic area(P=0.030) were statistically significant. No difference was shown in sex, age,gastrectomy type, tumor location, and clinical T, N and M stage.Conclusions: Our study found that the IPA originates from GDA, ASPDA and RGEA in similar proportions.Laparoscopic surgery may be more helpful in dissection of the IPA than open surgery. | Rulin Miao Jianjun Qu Zhengrong Li Daguang Wang Jiang Yu Weidong Zang Yong Li Fenglin Liu Jian Zhang Wu Song Kai Ye Su Yan Wei Wang Shuangyi Ren Lu Zang Changqing Jing Li Zhang Kuan Wang Weihua Fu Lin Fan Bin Liang Gang Zhao Jun Cai Li Yang Jiaming Zhu Jun You Kun Yang Qingxing Huang Zhaojian Niu Ning Ning Xingfeng Qiu Gang Ji Feng Liang Hua Huang Chao Gao Fei Shan Shuangxi Li Yongning Jia Lianhai Zhang Xiangji Ying Yan Zhang Zhaode Bu Xiangqian Su Gang Zhao Ziyu Li Jiafu Ji | 2018 | Chinese Journal of Cancer Research2018，30，5： | 5 |
| 3 | Laparoscopic vs.open lower mediastinal lymphadenectomy for Siewert typeⅡ/Ⅲadenocarcinoma of esophagogastric junction:An exploratory,observational,prospective,IDEAL stage2b cohort study(CLASS-10 study)显示文摘Objective:This study aims to verify the feasibility and efficacy of laparoscopic lower mediastinal lymphadenectomy for Siewert typeⅡ/Ⅲadenocarcinoma of esophagogastric junction(AEG).Setting:An exploratory,observational,prospective,cohort study will be carried out under the Idea,Development,Exploration,Assessment and Long-term Follow-up(IDEAL)framework(stage 2 b).Paritcipants:The study will recruit 1,036 patients with cases of locally advanced AEG(Siewert typeⅡ/Ⅲ,clinical stage cT2-4 aN0-3 M0),and 518 will be assigned to either the laparoscopy group or the open group.Interventions:Patients will receive lower mediastinal lymphadenectomy along with either total or proximal gastrectomy.Primary and secondary outcome measures:The primary endpoint is the number of lower mediastinal lymph nodes retrieved,and the secondary endpoints are the surgical safety and prognosis,including intraoperative and postoperative lower-mediastinal-lymphadenectomy-related morbidity and mortality,rate of rehospitalization,R0 resection rate,3-year local recurrence rate,and 3-year overall survival.Conclusions:The study will provide data for the guidance and development of surgical treatment strategies for AEG.Trial registration number:The study has been registered in ClinicalTrials.gov(No.NCT04443478). | Shuangxi Li Xiangji Ying Fei Shan Yongning Jia Zhemin Li Kan Xue Rulin Miao Yinkui Wang Zhaode Bu Xiangqian Su Ziyu Li Jiafu Ji | 2022 | Chinese Journal of Cancer Research2022，34，4： | 3 |
| 4 | SUB-NANOMETER POLISHING OF MAGNETIC RIGID DISK HEADS TO AVOID POLE TIP RECESSION显示文摘For the purpose of solving the problem that too large pole tip recession (PTR) is produced in magnetic rigid disk beads by mechanical polishing,a chemical mechanical nano-grinding experiment is performed by using a float-piece polisher with a tin plate to achieve a more plane and smoother surface. A basal solution, addition agents and a range of pH value are suitably selected to find a kind of slurry, with which the PTR can be controlled on sub-nanometer scale and the giant magnetic resistance (GMR) corrosion and electrostatic damage (ESD) can be avoided. Moreover, the cause that TiC protrudes from the substrate surface of the heads is studied. The appropriate shape and size of diamond abrasive are selected according to the chemical activation of Al_2O_3 and TiC in the same slurry. In this way, the chemical and mechanical interactions are optimized and the optimal surface that has small PTR and TiC asperity is achieved. Ultimatily, the chemical mechanical nano-grinding in combination with mechanical nano-grinding is adopted. Sub-nanometer PTR is achieved and the TiC asperity is eliminated by the chemical mechanical nano-grinding with large size of monocrystalline followed by mechanical nano-grinding with smalle polycrystalline diamonds. | SHEN Rulin ZHOU Li ZHONG Jue | 2008 | Chinese Journal of Mechanical Engineering2008，21，4： | 2 |
| 5 | WHIRLY1 recruits the histone deacetylase HDA15 repressing leaf senescence and flowering in Arabidopsis显示文摘Leaf senescence is controlled by a complex regulatory network in which robustness is ensured by the activity of transcription factors and epigenetic regulators.However,how these coordinate the process of leaf senescence remains poorly understood.We found that WHIRLY1 interacts with Histone Deacetylase(HDA)15,a Reduced Potassium Dependence3(RPD3)/HDA1-type HDA,by using green fluorescent protein-nanotrap-mass spectrum assays.The development-dependent interaction between WHIRLY1 and HDA15 was further confirmed by bimolecular fluorescence complementation assays and co-immunoprecipitation assays in Arabidopsis.Multi-omics genome-wide transcriptome and H3K9 acetylome enrichment analysis showed that HDA15 delays leaf senescence and flowering by repressing the expression of the positive regulators of leaf senescence and flowering,such as LOX2 and LARP1 C,and reducing H3K9 ac levels at these loci;WHIRLY1 and HDA15 co-target to the region near the transcription start site of a subset of nutrient recycling-related genes(e.g.,Glutathione S-transferases 10,non-coding RNA,and photosystem II protein D1 synthesizer attenuator PDIL1-2),as well as WRKY53 and ELF4,and co-repress their expression by removing H3K9 acetylation.Our study revealed a key transcription regulatory node of nutrient recycling and senescence-associated genes involved in leaf senescence and flowering via the recruitment of HDA15 by the single-stranded DNA/RNA-binding protein WHIRLY1. | Dongmei Huang Wei Lan Weibo Ma Rulin Huang Wenfang Lin Mengsi Li Chia-Yang Chen Keqiang Wu Ying Miao | 2022 | Journal of Integrative Plant Biology2022，64，7： | 2 |
| 6 | Determination of torasemide in human plasma and its bioequivalence study by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry显示文摘A sensitive and selective method using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry(HPLC–ESI–MS) to determine the concentration of torasemide in human plasma samples was developed and validated. Tolbutamide was chosen as the internal standard(IS). The chromatography was performed on a Gl Sciences Inertsil ODS-3 column(100 mm*2.1 mm i.d., 5.0μm) within 5 min, using methanol with 10 mM ammonium formate(60:40, v/v) as mobile phase at a fl ow rate of 0.2 mL/min. The targeted compound was detected in negative ionization at m/z 347.00 for torasemide and 269.00 for IS. The linearity range of this method was found to be within the concentration range of 1–2500 ng/mL(r=0.9984) for torasemide in human plasma. The accuracy of this measurement was between 94.05% and 103.86%. The extracted recovery ef fi ciency was from 84.20% to 86.47% at three concentration levels. This method was also successfully applied in pharmacokinetics and bioequivalence studies in Chinese volunteers. | Lin Zhang Rulin Wang Yuan Tian Zunjian Zhang | 2016 | Journal of Pharmaceutical Analysis2016，6，2： | 2 |
| 7 | TEM Study on Precipitation and Transformation of Secondary Carbides in 16Cr-1Mo-1Cu Wwhite Iron Subjected to Sub-Critical Treatment显示文摘 | Zhiping Sun Rulin Zuo Cong Li | 2004 | Materials Characterization2004，53，5： | 1 |
| 8 | Tile genetic epidemiology research of pri- mary liver cancer 显示文摘 | Meng Hui Liu Hongyan Cai Rulin Jiang Feng Jiang Qing five Lin Wenyao Shen Fumin | 2002 | Chinese journal of epidemiology2002，，6： | 1 |
| 9 | Time-dependent density-functional theory for open electronic systems显示文摘Time-dependent density-functional theory(TDDFT)has been successfully applied to predict excited-state properties of isolated and periodic systems.However,it cannot address a system coupled to an environment or whose number of electrons is not conserved.To tackle these problems,TDDFT needs to be extended to accommodate open systems.This paper provides a comprehensive account of the recent developments of TDDFT for open systems(TDDFT-OS),including both theoretical and practical aspects.The practicality and accuracy of a latest TDDFT-OS method is demonstrated with two numerical examples:the time-dependent electron transport through a series of quasi-one-dimensional atomic chains,and the real-time electronic dynamics on a two-dimensional graphene surface.The advancement of TDDFT-OS may lead to promising applications in various fields of chemistry,including energy conversion and heterogeneous catalysis. | ZHENG Xiao WANG RuLin | 2014 | Science China Chemistry2014，57，1： | 1 |
| 10 | Economic balance of crops and fruits production by K, Mg and S fertilizers application in subtropical red acid soil of Guangxi province显示文摘 | Hongwei Tan Liuqiang Zhou Rulin Xie | 2004 | TROPICS2004，13，4： | 1 |
| 11 | Better Sugarcane Production Acidic Red Soils 显示文摘 | Hongwei Tan Liuqiang Zhou Rulin Xie | 2005 | Better Crops with Plant Food2005，，3： | 1 |
| 12 | Macroscale and microscale analyses of nitrification and denitrification in biofilms attached on mem- brane aerated biofilm reactors 显示文摘 | Satoh H Ono H Rulin B | 2004 | Water Research2004，38，6： | 1 |
| 13 | Effect of fertilizer application and the main nutrient limiting factors for yield and quality of sugarcane production in Guangxi red soil 显示文摘 | Hongwei Tan Liuqiang Zhou Rulin Xie | 2005 | TROPICS2005，14，4： | 1 |
| 14 | Macroscale and microscale analyses of nitrification and denitrification in biofilms attached on membrane aerated biofilm reactors 显示文摘 | Satoh H Ono H Rulin B | 2004 | Water Research2004，38，6： | 1 |
| 15 | Comparison of ESI–and APCI–LC–MS/MS methods:A case study of levonorgestrel in human plasma显示文摘Electrospray ionization(ESI) and atmospheric pressure chemical ionization(APCI) techniques for liquid chromatography–tandem mass spectrometry(LC–MS/MS) determination of levonorgestrel were evaluated.In consideration of difference in ionization mechanism,the two ionization sources were compared in terms of LC conditions,MS parameters and performance of method.The sensitivity for detection of levonorgestrel with ESI was 0.25 ng/m L which was lower than 1 ng/m L with APCI.Matrix effects were evaluated for levonorgestrel and canrenone(internal standard,IS) in human plasma,and the results showed that APCI source appeared to be slightly less liable to matrix effect than ESI source.With an overall consideration,ESI was chosen as a better ionization technique for rapid and sensitive quantification of levonorgestrel.The optimized LC–ESI–MS/MS method was validated for a linear range of 0.25–50 ng/m L with a correlation coefficient ≥0.99.The intra-and inter-batch precision and accuracy were within 11.72% and 6.58%,respectively.The application of this method was demonstrated by a bioequivalence study following a single oral administration of 1.5 mg levonorgestrel tablets in 21 Chinese healthy female volunteers. | Rulin Wang Lin Zhang Zunjian Zhang Yuan Tian | 2016 | Journal of Pharmaceutical Analysis2016，6，6： | 1 |
| 16 | Prevalence and patterns of Y chromosome microdeletion in infertile men with azoospermia and oligzoospermia in Northeast China 显示文摘 | Elfateh F Rulin D Xin Y | 2014 | I- ran J Reprod Med2014，12，： | 1 |
| 17 | Macro scale and micro scale analyses of nitrification and denitrification in biofilms attached on membrane aerobicbiofilm reactors 显示文摘 | Hisashi Satoh Bian Rulin Jyn Kamo | 2004 | Water Research2004，38，6： | 1 |
| 18 | Relationship between retained austenite content,hardness and abrasion resistance of 16Cr-3C white iron显示文摘 | Sun Zhiping Zuo Rulin Li Cong | 2004 | Journal of University of Science and Technology Beijing2004，11，6： | 1 |
| 19 | Removal of humic substances by UF and NF membrane systems显示文摘 | Rulin Bian | 1999 | Wat Sci Tech1999，40，9： | 1 |
| 20 | Adnexal masses in postmenopausal women显示文摘 | Preston A | 1987 | Obstet Gynecol1987，70，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员