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| 1 | Development of T. aestivum L.eH. californicum Alien Chromosome Lines and Assignment of Homoeologous Groups of Hordeum californicum Chromosomes显示文摘Hordeum californicum(2n=2x=14, HH) is resistant to several wheat diseases and tolerant to lower nitrogen. In this study, a molecular karyotype of H. californicum chromosomes in the Triticum aestivum L. cv. Chinese Spring(CS)eH. californicum amphidiploid(2n=6x=56, AABBDDHH) was established. By genomic in situ hybridization(GISH) and multicolor fluorescent in situ hybridization(FISH) using repetitive DNA clones(pTa71, pTa794 and pSc119.2) as probes, the H. californicum chromosomes could be differentiated from each other and from the wheat chromosomes unequivocally. Based on molecular karyotype and marker analyses, 12 wheatealien chromosome lines, including four disomic addition lines(DAH1, DAH3, DAH5 and DAH6), five telosomic addition lines(MtH7L,MtH1 S, MtH1 L, DtH6 S and DtH6L), one multiple addition line involving H. californicum chromosome H2, one disomic substitution line(DSH4) and one translocation line(TH7S/1BL), were identified from the progenies derived from the crosses of CSeH. californicum amphidiploid with common wheat varieties. A total of 482 EST(expressed sequence tag) or SSR(simple sequence repeat) markers specific for individual H. californicum chromosomes were identified, and 47, 50, 45, 49, 21, 51 and 40 markers were assigned to chromosomes H1, H2, H3, H4, H5, H6 and H7, respectively. According to the chromosome allocation of these markers, chromosomes H2,H3, H4, H5, and H7 of H. californicum have relationship with wheat homoeologous groups 5, 2, 6, 3, and 1, and hence could be designated as 5Hc, 2Hc, 6Hc, 3Hcand 1Hc, respectively. The chromosomes H1 and H6 were designated as 7Hcand 4Hc, respectively, by referring to SSR markers located on rye chromosomes. | Yuhui Fang Jingya Yuan Zhangjun Wang Haiyan Wang Jin Xiao Zhixi Yang Ruiqi Zhang Zengjun Qi Weigang Xu Lin Hu Xiu-E Wang | 2014 | Journal of Genetics and Genomics2014，41，8： | 2 |
| 2 | Moderate papain addition improves the physicochemical,microbiological,flavor and sensorial properties of Chouguiyu,traditional Chinese fermented fish显示文摘The objective of this study was to investigate the effect of papain on the physicochemical and microbiological properties,volatile compound profiles,and sensorial attributes of Chouguiyu.Papain was applied at a dose of 10,50,200,400 U/g fish.Results showed that papain addition improved the release of free amino acids,essential amino acids,eicosapentaenoi acid(EPA,C20:5n3),and docosapentenoic acid(DPA,C22:5n6),respectively.Lower biogenic amines content,higher the volatile compound concentrations and relative abundance of Lactobacillales were also observed.Further results showed that the papain dose group decreased the fermentation time from 10 to 7 days while good organoleptic quality was remained,especially in the 200 U/g dose group.The redundancy analysis(RDA)showed a high correlation between Lactobacillus and the key volatile compounds in the 200 U/g and 400 U/g groups,which indicated that Lactobacillus could contribute to the fatty flavor.In summary,proper papain addition(i.e.200 U/g or 400 U/g)could reduce biogenic amines contents and maintain acceptable sensorial properties of Chouguiyu in a shorter fermentation time. | Wenhuan Xu Cuicui Jiang Aoxue Liu Ruiqi Bao Wenqing Wang Zuoli Zhang Chaofan Ji Huipeng Liang Sufang Zhang Xinping Lin | 2022 | Food Bioscience2022，46，2： | 2 |
| 3 | Deep learning for non-parameterized MEMS structural design显示文摘The geometric designs of MEMS devices can profoundly impact their physical properties and eventual performances.However,it is challenging for researchers to rationally consider a large number of possible designs,as it would be very time-and resource-consuming to study all these cases using numerical simulation.In this paper,we report the use of deep learning techniques to accelerate the MEMS design cycle by quickly and accurately predicting the physical properties of numerous design candidates with vastly different geometric features.Design candidates are represented in a nonparameterized,topologically unconstrained form using pixelated black-and-white images.After sufficient training,a deep neural network can quickly calculate the physical properties of interest with good accuracy without using conventional numerical tools such as finite element analysis.As an example,we apply our deep learning approach in the prediction of the modal frequency and quality factor of disk-shaped microscale resonators.With reasonable training,our deep learning neural network becomes a high-speed,high-accuracy calculator:it can identify the flexural mode frequency and the quality factor 4.6×10^(3)times and 2.6×10^(4)times faster,respectively,than conventional numerical simulation packages,with good accuracies of 98.8±1.6%and 96.8±3.1%,respectively.When simultaneously predicting the frequency and the quality factor,up to~96.0%of the total computation time can be saved during the design process.The proposed technique can rapidly screen over thousands of design candidates and promotes experience-free and data-driven MEMS structural designs. | Ruiqi Guo Fanping Sui Wei Yue Zekai Wang Sedat Pala Kunying Li Renxiao Xu Liwei Lin | 2022 | Microsystems & Nanoengineering2022，8，4： | 2 |
| 4 | Rabies Virus Pseudotyped with CVS-N2C Glycoprotein as a Powerful Tool for Retrograde Neuronal Network Tracing显示文摘Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain. The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2 C glycoprotein(SAD-RV(DG)-N2 C(G)) shows strong neuro-tropism in cell culture, but its in vivo efficiency for retrograde gene transduction and neuro-tropism have not been systematically characterized.We compared these features in different mouse brain regions for SAD-RV-N2 C(G) and two other widely-used retrograde tracers, SAD-RV(DG)-B19(G) and r AAV2-retro. We found that SAD-RV(DG)-N2 C(G) enhanced the infection efficiency of long-projecting neurons by^10 times but with very similar neuro-tropism, compared with SAD-RV(DG)-B19(G). On the other hand, SAD-RV(DG)-N2 C(G) had an infection efficiency comparable with r AAV2-retro, but a more restricted diffusion range, and broader tropism to different types and regions of longprojecting neuronal populations. These results demonstrate that SAD-RV(DG)-N2 C(G) can serve as an effective retrograde vector for studying neuronal circuits. | Xutao Zhu Kunzhang Lin Qing Liu Xinpei Yue Huijie Mi Xiaoping Huang Xiaobin He Ruiqi Wu Danhao Zheng Dong Wei Liangliang Jia Weilin Wang Anne Manyande Jie Wang Zhijian Zhang Fuqiang Xu | 2020 | Neuroscience Bulletin2020，36，3： | 2 |
| 5 | Preparation of copolymer-grafted mixed-mode resins for immunoglobulin G adsorption显示文摘The mixed-mode resins for protein adsorption have been prepared by a novel strategy, copolymer grafting. Specially, the copolymer-grafted resins CG-MA with two functional groups, 5-amino-benzimidazole (ABI) and methacryloxyethyltrimethyl ammonium chloride (METAC), have been prepared through surfaceinitiated activator generated by electron transfer for atom transfer radical polymerization of METAC and glycidyl methacrylate (GMA), followed by a ring-open reaction to introduce ABI. The charge and hydrophobicity of CG-MA resins could be controlled by manipulating the addition of METAC and GMA/ABI. Besides, METAC and ABI provided positive effects together in both protein adsorption and elution: dynamic binding capacity of human Immunoglobulin G (hlgG) onto CG-M-A resin with the highest ligand ratio of METAC to ABI is 46.8 mg-g 1 at pH 9 and the elution recovery of hlgG is 97.0% at pH 5. The separation experiment showed that purity and recovery of monoclonal antibody from cell culture supernatant are 96.0% and 86.5%, respectively, indicating that copolymer-grafted mixed-mode resins could be used for antibody purification. | Shenggang Chen Tao Liu Ruiqi Yang Dongqiang Lin Shanjing Yao | 2019 | Frontiers of Chemical Science and Engineering2019，13，1： | 1 |
| 6 | Experimental investigation on an adsorption system for producing chilled water显示文摘 | Zhu Ruiqi Han Baoqi Lin Meizhen | 1992 | Int J Refrig1992，15，1： | 1 |
| 7 | Experimerltal investigation on an adsorption system forprodueing chilled water显示文摘 | Zhu Ruiqi Han Baoqi Lin Meizhen | 1992 | International Journal of Refrigeration1992，15，1： | 1 |
| 8 | Identification of two pIgR-like molecules in teleost fish with opposite roles in mucosal immunity against bacterial infection显示文摘Polymeric immunoglobulin(Ig)receptor-like(pIgRL)molecules have been identified in teleost fish.However,compared to functional studies of their related genes(e.g.,mammalian CD300 family)in eliminating pathogen invasion while preserving homeostasis,the roles of pIgRL in teleost fish remain unclear.In this study,we demonstrated that a pair of pIgRL molecules in zebrafish,pIgRL3.5 and pIgRL4.2,were highly expressed in the intestine and immune cells.Moreover,we constructed an Edwardsiella piscicida infection model,which induced strong inflammatory responses in the zebrafish intestine.Interestingly,pIgRL3.5 and pIgRL4.2 exhibited opposite inducible expression patterns in response to bacterial infection,suggesting that they perform different roles.More importantly,by conducting overexpression and knockdown experiments,our findings demonstrated that zebrafish pIgRL3.5 played a protective role in host defense against E.piscicida infection by inhibiting excessive inflammatory responses.In contrast,pIgRL4.2 facilitated pathogen growth and dissemination in zebrafish intestine.Collectively,our findings are the first to demonstrate that a pair of pIgRL molecules in teleost fish play opposite roles in mucosal immune response to bacterial infection.Therefore,our results provide crucial insights into the conserved role of pIgRL molecules in immune regulatory functions throughout vertebrate evolution. | Haoyue Xu Zixuan Wang Zhenyu Huang Xiaoyun Chen Ruiqi Lin Yongyao Yu Zhen Xu | 2023 | Water Biology and Security2023，2，4： | 0 |
| 9 | Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium显示文摘Retinal pigment epithelium(RPE)has essential functions,such as nourishing and supporting the neural retina,and is of vital importance in the pathogenesis of age-related retinal degeneration.However,the exact molecular changes of RPE during aging remain poorly understood.Here,we isolated human primary RPE(h RPE)cells from 18 eye donors distributed over a wide age range(10–67 years old).A quantitative proteomic analysis was performed to analyze changes in their intracellular and secreted proteins.Age-group related subtypes and age-associated proteins were revealed and potential age-associated mechanisms were validated in ARPE-19 and h RPE cells.The results of proteomic data analysis and verifications suggest that RNF123-and RNF149-related protein ubiquitination plays an important role in protecting h RPE cells from oxidative damage during aging.In older h RPE cells,apoptotic signaling-related pathways were up-regulated,and endoplasmic reticulum organization was down-regulated both in the intracellular and secreted proteomes.Our work paints a detailed molecular picture of h RPE cells during the aging process and provides new insights into the molecular characteristics of RPE during aging and under other related clinical retinal conditions. | Xiuxiu Jin Jingyang Liu Weiping Wang Jiangfeng Li Guangming Liu Ruiqi Qiu Mingzhu Yang Meng Liu Lin Yang Xiaofeng Du Bo Lei | 2022 | Genomics, Proteomics & Bioinformatics2022，20，4： | 0 |
| 10 | Extraction and imaging of indicator elements for non-destructive,in-situ,fast identification of adverse geology in tunnels显示文摘The lag in quantitative methods and detection techniques for geologic information has resulted in time-consuming and human-experienced geologic analysis in tunnels.Geochemical indicators of rocks can be used to identify adverse geology and to explain the intrinsic causes of damage to normal rocks.This study proposes a method to identify adverse geology by extracting and imaging the indicator elements.The mapping relationship between rock components and geologic bodies is quickly determined by indicator element extraction based on factor analysis,and then the data are gridded for image output.The location and size of the target adverse geology are visually identified through the distribution images of the indicator elements,thus reducing data dimensions and analysis time.A non-destructive,in-situ and fast element detection technique in tunnels was adopted to speed up the process of geology identification.The accuracy of the detection was validated by comparing field and laboratory test results.This study further confirms and refines the previous research,and the results provide references for geological,mining and underground projects. | Fumin Liu Peng Lin Zhenhao Xu Ruiqi Shao Tao Han | 2023 | International Journal of Mining Science and Technology2023，33，12： | 0 |
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