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| 1 | Proteome analysis of peroxisomes from dark-treated senescent Arabidopsis leaves显示文摘Peroxisomes compartmentalize a dynamic suite of biochemical reactions and play a central role in plant metabolism, such as the degradation of hydrogen peroxide,metabolism of fatty acids, photorespiration, and the biosynthesis of plant hormones. Plant peroxisomes have been traditionally classified into three major subtypes, and in-depth mass spectrometry(MS)-based proteomics has been performed to explore the proteome of the two major subtypes present in green leaves and etiolated seedlings. Here, we carried out a comprehensive proteome analysis of peroxisomes from Arabidopsis leaves given a 48-h dark treatment.Our goal was to determine the proteome of the third major subtype of plant peroxisomes from senescent leaves, and further catalog the plant peroxisomal proteome. We identifieda total of 111 peroxisomal proteins and verified the peroxisomal localization for six new proteins with potential roles in fatty acid metabolism and stress response by in vivo targeting analysis. Metabolic pathways compartmentalized in the three major subtypes of peroxisomes were also compared, which revealed a higher number of proteins involved in the detoxification of reactive oxygen species in peroxisomes from senescent leaves. Our study takes an important step towards mapping the ful function of plant peroxisomes. | Ronghui Pan Sigrun Reumann Piotr Lisik Stefanie Tietz Laura J.Olsen Jianping Hu | 2018 | Journal of Integrative Plant Biology2018，60，11： | 5 |
| 2 | In-depth proteome analysis of Arabidopsis leaf peroxisomes combined with in vivo subcellular targeting verification indicates novel metabolic and regulatory functions of peroxisomes 显示文摘 | REUMANN S QUAN S AUNG K | 2009 | Plant Physiol2009，150，： | 1 |
| 3 | Pentamethylcyclopentadienylgallium ( Cp* Ga ): alternatire synthesis and application as a terminal and bridging ligand in the chemistry of chromium, iron, cobalt, and nickel 显示文摘 | Jutzi P Neumann B Reumann G | 1998 | Organometallics1998，17，7： | 1 |
| 4 | New guidelines for fluorophore application in peroxisome targeting analyses in transient plant expression systems显示文摘Peroxisome research has been revolutionized by proteome studies combined with in vivo subcellular targeting analyses. Yellow and cyan fluorescent protein(YFP and CFP) are the classical fluorophores of plant peroxisome research. In the new transient expression system of Arabidopsis seedlings co-cultivated with Agrobacterium we detected the YFP fusion of one candidate protein in peroxisomes, but only upon co-transformation with the peroxisome marker, CFP-PTS1. The data suggested that the YFP fusion was directed to peroxisomes due to its weak heterodimerization ability with CFP-PTS1,allowing piggy-back import into peroxisomes. Indeed, if co-expressed with monomeric Cerulean-PTS1(mCer-PTS1),the YFP fusion was no longer matrix localized. We systematically investigated the occurrence and extent of dimerization-based piggy-back import for different fluorophore combinations in five major transient plant expression systems. In Arabidopsis seedlings and tobacco leaves both untagged YFP and monomeric Venus were imported into peroxisomes if co-expressed with CFP-PTS1 but not with mCer-PTS1. By contrast, piggy-back import of cytosolic proteins was not observed in Arabidopsis and tobacco protoplasts or in onion epidermal cells for any fluorophore combination at any time point. Based on these important results we formulate new guidelines for fluorophore usage and experimental design to guarantee reliable identification of novel plant peroxisomal proteins. | Christian Falter Nguyen Binh Anh Thu Saugat Pokhrel Sigrun Reumann | 2019 | Journal of Integrative Plant Biology2019，61，7： | 1 |
| 5 | Advances and futuredirections for management of trauma patients with musculoskeletalinjurieS显示文摘 | Balogh ZJ Reumann MK Gruen RL | 2012 | Lancet2012，380，： | 1 |
| 6 | Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms 显示文摘 | Reumann S Babujee L Ma C | 2007 | The Plant Ce112007，19，： | 1 |
| 7 | Computer model for the optimization of AV andiron delay in cardiac resynchronization therapy显示文摘 | Reumann M Farina D Miri R | 2007 | Med Boil Eng Comput2007，45，9： | 1 |
| 8 | AraPerox a database of putative arabidopsis proteins from plant peroxisomes显示文摘 | REUMANN S MA C LEMKE S | 2004 | Plant Physiology2004，136，1： | 1 |
| 9 | Molecular imaging of expression of vascular endothelial growth factor a(VEGF a)in femoral bone grafts transplanted into living mice显示文摘 | STRACHNA O TORRECILLA D REUMANN M K | 2014 | Cell Transplant2014，23，7： | 1 |
| 10 | Molecular characterization of the human La protein hepatitis B virus RNA B interaction in vitro显示文摘 | Horke S Reumann K Rang A | 2002 | J Biol Chem2002，277，34： | 1 |
| 11 | Plant peroxisomes:Biogenesis and function显示文摘 | Hu J Baker A Bartel B Linka N Mullen RT Reumann S Zolman BK | | 0，，： | 1 |
| 12 | A database of putative Arabidopsis proteins from plant peroxisomes显示文摘 | Reumann S Changle M A Lemke S | 2004 | Plant Physiol2004，136，： | 1 |
| 13 | Molecular imaging of expression of vascular endothelial growth factor a(VEGF A)in femoral bone grafts transplanted into living mice显示文摘 | Strachna O Torrecilla D Reumann MK | 2014 | Cell Transpl2014，23，7： | 1 |
| 14 | Peroxisome biogenesis and function显示文摘 | Kaur N Reumann S Hu J | | 0，，： | 1 |
| 15 | Computer model for the optimization of AV andiron delay in cardiac resynchronization therapy显示文摘 | REUMANN M FARINA D MIRI R | 2007 | Med Boil Eng Comput2007，45，： | 1 |
| 16 | Specification of the peroxisome targeting signals type 1 and type 2 of plant peroxisomes by bioinformatics analyses 显示文摘 | Reumann S | 2004 | Plant Physiology2004，135，2： | 1 |
| 17 | A database of putative Arabidopsis proteins from plant peroxisomes 显示文摘 | Reumann S Ma C Lemke S Babujee L AraPerox | 2004 | Plant Physiology2004，136，1： | 1 |
| 18 | Computer model for the optimization of AV and iron delay in cardiac resynchronization therapy显示文摘 | Reumann M Farina D Miri R | | 0，，09： | 1 |
| 19 | Optimizing Curve Segmentation in Computer Graphics 显示文摘 | REUMANN K WITKAM A | 1974 | Proceedings of International Computing Symposium1974，，31： | 1 |
| 20 | TGF-beta 1 as a pathophysiological factor in fracture healing显示文摘 | Zimmermann G Moghaddam A Reumann M | 2007 | Unfallchirurg2007，110，2： | 1 |
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