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| 1 | 3D printing of silk fibroin-based hybrid scaffold treated with platelet rich plasma for bone tissue engineering显示文摘3D printing/bioprinting are promising techniques to fabricate scaffolds with well controlled and patient-specific structures and architectures for bone tissue engineering.In this study,we developed a composite bioink consisting of silk fibroin(SF),gelatin(GEL),hyaluronic acid(HA),and tricalcium phosphate(TCP)and 3D bioprinted the silk fibroin-based hybrid scaffolds.The 3D bioprinted scaffolds with dual crosslinking were further treated with human platelet-rich plasma(PRP)to generate PRP coated scaffolds.Live/Dead and MTT assays demonstrated that PRP treatment could obviously promote the cell growth and proliferation of human adipose derived mesenchymal stem cells(HADMSC).In addition,the treatment of PRP did not significantly affect alkaline phosphatase(ALP)activity and expression,but significantly upregulated the gene expression levels of late osteogenic markers.This study demonstrated that the 3D printing of silk fibroin-based hybrid scaffolds,in combination with PRP post-treatment,might be a more efficient strategy to promote osteogenic differentiation of adult stem cells and has significant potential to be used for bone tissue engineering. | Liang Wei Shaohua Wu Mitchell Kuss Xiping Jiang Runjun Sun Reid Patrick Xiaohong Qin Duan Bin | 2019 | Bioactive Materials2019，4，1： | 6 |
| 2 | Unresectable Cholangiocarcinoma: Comparison of Survival in Biliary Stenting Alone Versus Stenting With Photodynamic Therapy显示文摘 | Michel Kahaleh Rajnish Mishra Vanessa M. Shami Patrick G. Northup Carl L. Berg Penny Bashlor Petra Jones Kristi Ellen Geoffrey R. Weiss Christiana M. Brenin Barbara E. Kurth Tyvin A. Rich Reid B. Adams Paul Yeaton | 2008 | Clinical Gastroenterology and Hepatology2008，，： | 1 |
| 3 | Use of opioid analgesics in the treatment of cancer pain: evidence-based recommendations from the EAPC显示文摘 | Augusto Caraceni Geoffrey Hanks Stein Kaasa Michael I Ben- nett Cirmia Brunelli Nathan Chemy Ola Dale Franco De Conno Marie Fallon Magdi Hanna Dagny Faksvfig Haugen Gitte Juhl Samuel King PadKlepstad Eivor A Laugsand Marco Maltoni Se- bastiano Mercadante Maria Nabal Alessandra Pigni Lukas Rad- bmch Colette Reid Per Sjogren Patrick C Stone Davide Tassi- nail Giovambattista Zeppetella | 2012 | Lancet Oncol2012，13，2： | 1 |
| 4 | Unresectable Cholangiocarcinoma: Comparison of Survival in Biliary Stenting Alone Versus Stenting With Photodynamic Therapy显示文摘 | Michel Kahaleh Rajnish Mishra Vanessa M. Shami Patrick G. Northup Carl L. Berg Penny Bashlor Petra Jones Kristi Ellen Geoffrey R. Weiss Christiana M. Brenin Barbara E. Kurth Tyvin A. Rich Reid B. Adams Paul Yeaton | 2008 | Clinical Gastroenterology and Hepatology2008，，3： | 1 |
| 5 | Role of subplate neurons in functional maturation of visual cortical columns显示文摘 | Patrick O Kanold Prakash Kara Clay Reid R | 2003 | Science2003，301，25： | 1 |
| 6 | Model for End-Stage Liver Disease (MELD) Predicts Nontransplant Surgical Mortality in Patients With Cirrhosis显示文摘 | Patrick G. Northup Ryan C. Wanamaker Vanessa D. Lee Reid B. Adams Carl L. Berg | 2005 | Annals of Surgery2005，，2： | 1 |
| 7 | Antiplatelets vs anticoagulation for dissection: CADISS nonrandomized arm and meta-analysis显示文摘 | Fiona Kennedy Silvia Lanfranconi Cara Hicks John Reid Patrick Gompertz Christopher Price Sally Kerry John Norris Hugh S. Markus | 2012 | Neurology2012，，7： | 1 |
| 8 | Tumour suppressor microRNAs contribute to drug resistance in malignant pleural mesothelioma by targeting anti-apoptotic pathways显示文摘Aim:Aberrant microRNA expression is a common event in cancer drug resistance,however its involvement in malignant pleural mesothelioma(MPM)drug resistance is largely unexplored.We aimed to investigate the contribution of microRNAs to the resistance to drugs commonly used in the treatment of MPM.Methods:Drug resistant MPM cell lines were generated by treatment with cisplatin,gemcitabine or vinorelbine.Expression of microRNAs was quantified using RT-qPCR.Apoptosis and drug sensitivity assays were carried out following transfection with microRNA mimics or BCL2 siRNAs combined with drugs.Results:Expression of miR-15a,miR-16 and miR-34a was downregulated in MPM cells with acquired drug resistance.Transfection with miR-15a or miR-16 mimics reversed the resistance to cisplatin,gemcitabine or vinorelbine,whereas miR-34a reversed cisplatin and vinorelbine resistance only.Similarly,in parental cell lines,miR-15a or miR-16 mimics sensitised cells to all drugs,whereas miR-34a increased response to cisplatin and vinorelbine.Increased microRNA expression increased drug-induced apoptosis and caused BCL2 mRNA and protein reduction.RNAi-mediated knockdown of BCL2 partly recapitulated the increase in drug sensitivity in cisplatin and vinorelbine treated cells.Conclusion:Drug-resistant MPM cell lines exhibited reduced expression of tumour suppressor microRNAs.Increasing tumour suppressor of microRNA expression sensitised both drug resistant and parental cell lines to chemotherapeutic agents,in part through targeting of BCL2.Taken together,these data suggest that miR-15a,miR-16 and miR-34a are involved in the acquired and intrinsic drug resistance phenotype of MPM cells. | Marissa Williams Yuen Yee Cheng Monica Phimmachanh Patrick Winata Nico van Zandwijk Glen Reid | 2019 | Cancer Drug Resistance2019，2，4： | 0 |
| 9 | Pathological factors affecting gastric adenocarcinoma survival in a Caribbean population from 2000-2010显示文摘AIM: To investigate pathological factors related to long term patient survival post surgical management of gas-tric adenocarcinoma in a Caribbean population.METHODS: This is a retrospective, observational study of all patients treated surgically for gastric adenocarci-noma from January 1st 2000 to December 31 st 2010 at The University Hospital of the West Indies, an urban Jamaican hospital. Pathological reports of all gastrecto-my specimens post gastric cancer resection during the specified interval were accessed. Patients with a final diagnosis other than adenocarcinoma, as well as pa-tients having undergone surgery at an external institu-tion were excluded. The clinical records of the selected cohort were reviewed. The following variables were analysed; patient gender, patient age, the number of gastrectomies previous performed by the lead surgeon, the gross anatomical location and appearance of the tumour, the histological appearance of the tumour, infil-tration of the tumour into stomach wall and surround-ing structures, presence of Helicobacter pylori and the presence of gastritis. Patient status as dead vs alive was documented for the end of the interval. The effect of the aforementioned factors on patient survival were analysed using Logrank tests, Cox regression models, Ranksum tests, Kruskal-Wallis tests and Kaplan-Meier curves.RESULTS: A total of 79 patients, 36 males and 43 fe-males, were included. Their median age was 67 years(range 36-86 years). Median survival time from surgery was 70 mo with 40.5% of patients dying before the termination date of the study. Tumours ranged from 0.8 cm in size to encompassing the entire stomach speci-men, with a median tumour size of 6 cm. The median number of nodes removed at surgery was 8 with a maximum of 28. The median number of positive lymph nodes found was 2, with a range of 0 to 22. Patients' median survival time was approximately 70 mo, with 40.5% of the patients in this cohort dying before the terminal date. An increase in the incidence of cardiac tumours was noted compared to the previous 10 year interval(7.9% to 9.1%). Patients who had serosal involvement of the tumour did have a significantly shorter survival than those who did not(P = 0.017). A significant increase in the hazard ratio(HR), 2.424, for patients with circumferential tumours was found(P = 0.044). Via Kaplan-Meier estimates, the presence of venous infiltration as well as involvement of the circum-ferential resection margin were found to be poor prog-nostic markers, decreasing survival at 50 mo by 46.2% and 36.3% respectively. The increased HR for venous infiltration, 2.424, trended toward significant(P = 0.055) Age, size of tumour, number of positive nodes found and total number of lymph nodes removed were not useful predictors of survival. It is noted that the results were mostly negative, that is many tumour character-istics did not indicate any evidence of affecting patient survival. The current sample, with 30 observed events(deaths), would have about 30% power to detect a HR of 2.5.CONCLUSION: This study mirrors pathological factors used for gastric cancer prognostication in other popu-lations. As evaluation continues, a larger cohort will strengthen the significance of observed trends. | Patrick O Roberts Joseph Plummer Pierre-Anthony Leake Shane Scott Tamara G de Souza Ayesha Johnson Tracey N Gibson Barrie Hanchard Marvin Reid | 2014 | World Journal of Gastrointestinal Surgery2014，6，6： | 0 |
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