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| 1 | Value of a newly sequenced bacterial genome显示文摘Next-generation sequencing(NGS) technologies have made high-throughput sequencing available to medium- and small-size laboratories, culminating in a tidal wave of genomic information. The quantity of sequenced bacterial genomes has not only brought excitement to the field of genomics but also heightened expectations that NGS would boost antibacterial discovery and vaccine development. Although many possible drug and vaccine targets have been discovered, the success rate of genome-based analysis has remained below expectations. Furthermore, NGS has had consequences for genome quality, resulting in an exponential increase in draft(partial data) genome deposits in public databases. If no further interests are expressed for a particular bacterial genome, it is more likely that the sequencing of its genome will be limited to a draft stage, and the painstaking tasks of completing the sequencing of its genome and annotation will not be undertaken. It is important to know what is lost when we settle for a draft genome and to determine the 'scientific value' of a newly sequenced genome. This review addresses the expected impact of newly sequenced genomes on antibacterial discovery and vaccinology. Also, it discusses the factors that could be leading to the increase in the number of draft deposits and the consequent loss of relevant biological information. | Eudes GV Barbosa Flavia F Aburjaile Rommel TJ Ramos Adriana R Carneiro Yves Le Loir Jan Baumbach Anderson Miyoshi Artur Silva Vasco Azevedo | 2014 | World Journal of Biological Chemistry2014，5，2： | 7 |
| 2 | Association of insertion-deletions polymorphisms with colorectal cancer risk and clinical features显示文摘AIM To investigate the association between 16 insertiondeletions(INDEL) polymorphisms, colorectal cancer(CRC) risk and clinical features in an admixed population.METHODS O n e h u n d re d a n d fo r ty p a t i e n t s w i t h C R C a n d 140 cancer-free subjects were examined. Genomic DNA was extracted from peripheral blood samples. Polymorphisms and genomic ancestry distribution were assayed by Multiplex-PCR reaction, separated by capillary electrophoresis on the ABI 3130 Genetic Analyzer instrument and analyzed on Gene Mapper ID v3.2. Clinicopathological data were obtained by consulting the patients' clinical charts, intra-operative documentation, and pathology scoring.RESULTS Logistic regression analysis showed that polymorphism variations in IL4 gene was associated with increased CRC risk, while TYMS and UCP2 genes were associated with decreased risk. Reference to anatomical localization of tumor Del allele of NFKB1 and CASP8 were associated with more colon related incidents than rectosigmoid. In relation to the INDEL association with tumor node metastasis(TNM) stage risk, the Ins alleles of ACE, HLAG and TP53(6 bp INDEL) were associated with higher TNM stage. Furthermore, regarding INDEL association with relapse risk, the Ins alleles of ACE, HLAG, and UGT1A1 were associated with early relapse risk, as well as the Del allele of TYMS. Regarding INDEL association with death risk before 10 years, the Ins allele of SGSM3 and UGT1A1 were associated with death risk.CONCLUSION The INDEL variations in ACE, UCP2, TYMS, IL4, NFKB1, CASP8, TP53, HLAG, UGT1A1, and SGSM3 were associated with CRC risk and clinical features in an admixed population. These data suggest that this cancer panel might be useful as a complementary tool for better clinical management, and more studies need to be conducted to confirm these findings. | Diego Marques Layse Raynara Ferreira-Costa Lorenna Larissa Ferreira-Costa Romualdo da Silva Correa Aline Maciel Pinheiro Borges Fernanda Ribeiro Ito Carlos Cesar de Oliveira Ramos Raul Hernandes Bortolin André Ducati Luchessi Andrea Ribeiro-dos-Santos Sidney Santos Vivian Nogueira Silbiger | 2017 | World Journal of Gastroenterology2017，23，37： | 2 |
| 3 | Clinical hematological and biochemical parameters in Swiss,BALB/c,C57BL/6 and B6D2F1 Mus musculus显示文摘Background:Animal models are widely used in scientific research in order to obtain information from a whole organism under a specific set of experimental conditions.Various lineages of mice have been used to investigate diseases and new therapeutic strategies,and,consequently,hematological and biochemical tests in these laboratory animals are essential to validate scientific studies.Our study seeks to establish reference values for hematological and biochemical parameters of four lineages of mice.Methods:We evaluated the hematological and biochemical profiles of 20 males and 20 females from the lineages Swiss(heterogeneous),BALB/c and C57BL/6(isogenic),and B6D2F1(hybrid),totaling 160 mice.Analysis were standardized using the systems pocH-100iV Diff™for 19 hematological parameters and VITROS®350 for 12 biochemical parameters.Results:Results are shown as means and standard deviation,grouped by lineage and genre.Comparing the values obtained in this study with the values from previous studies,some variations were detected,which could be explained by differences in methodologies or individual variability.Conclusion:Thus our study shows that knowledge and disclosure of the values of physiological parameters of laboratory animals is necessary,and emphasises the importance of considering variations influenced by gender,lineage and genotype in the choice of the best experimental model. | Giorgio Silva-Santana Juliet Cunha Bax Débora Cristina Silva Fernandes Daniela Tendler Leibel Bacellar Cleber Hooper Alexandre Alves Souza Oliveira Dias Cristina Barbosa Silva Aline Moreira de Souza Simone Ramos Ricardo Alexandre Santos Thainara Ramos Pinto Mariana Antunes Ramão Ana Luíza Mattos-Guaraldi | 2020 | Animal Models and Experimental Medicine2020，3，4： | 2 |
| 4 | First report of CTX-M producing Escherichia coli , including the new ST2526, isolated from beef cattle and sheep in Portugal显示文摘 | Sónia Ramos Gilberto Igrejas Nuno Silva Daniela Jones-Dias José-Luís Capelo-Martinez Manuela Cani?a Patrícia Poeta | 2013 | Food Control2013，，1： | 1 |
| 5 | Interfacial and colloidal behavior of asphaltenes obtained from Brazilian crude oils 显示文摘 | Antonio Carlos Da Silva Ramos Lilian Haraguchi Fabio R Notrispe | 2001 | Journal of Petroleum Science and Engineering2001，32，： | 1 |
| 6 | Ectopic thyroid presenting as a submandibular mass显示文摘 | BERSANETI J A SILVA R D RAMOS R R | 2011 | Head Neck Pathol2011，5，： | 1 |
| 7 | The nuclear topography of abl, bcr, PML and RARa genes : evidence for gene proximity in specific phases of the cell cycle and stages of hematopoietic differentiation显示文摘 | Neves H Ramos C da Silva M G | 1999 | Blood1999，93，： | 1 |
| 8 | High prevalence of antimicrobial-resistant Escherichia coli from animals at slaughter: A food safety risk显示文摘 | Ramos S Silva N Canica M | 2013 | Sci Food Agric2013，93，： | 1 |
| 9 | A prothrombin activator from Bothrops erythromelas (jararaca-da-seca) snake venom: characterization and molecular cloning显示文摘 | Silva MB Schattner M Ramos CR | 2003 | Biochem J2003，369，1： | 1 |
| 10 | Functional results of two different surgical ap- proaches in patients with distal humerus fractures type C (AO) 显示文摘 | Mejla Silva D Morales de los Santos R Cienega Ramos MA | 2008 | Acta Ortop Mex2008，22，1： | 1 |
| 11 | Functional results of two different surgical appruaches in patients with distal humans fractures type C (AO) 显示文摘 | Mejia Silva D Morales de los Santos R Cienega Ramos M A | 2008 | Acta Ortop Mex2008，，： | 1 |
| 12 | Quantification ofmicrostructural changes during first stage air drying of grape tissue显示文摘 | Ramos I N Silva C L Sereno A M | 2004 | Journal of Food Engineering2004，62，2： | 1 |
| 13 | Molecular typing of Mycobacterium bovis isolates: a review 显示文摘 | Ramos DF Tavares L da Silva PE | 2014 | Braz J Microbiol2014，45，2： | 1 |
| 14 | Effect of dexamethasone on development of in vitro –produced bovine embryos显示文摘 | Priscila P.B. Santana Carla M.F. Carvalho Nathália N. da Costa Thiago V.G. Silva Priscilla C.A. Ramos Marcela S. Cordeiro Simone S.D. Santos André S. Khayat Otávio M. Ohashi Moysés S. Miranda | 2014 | Theriogenology2014，，： | 1 |
| 15 | Chemical Composition, Leishmanicidal and Cytotoxic Activities of the Essential Oils from Mangifera indica L. var. Rosa and Espada显示文摘 | Eduardo H. S. Ramos Marcílio M. Moraes Laís L. de A. Nerys Silene C. Nascimento Gardênia C. G. Milit?o Regina C. B. Q. de Figueiredo Cláudio A. G. da Camara Teresinha Gon?alves Silva Silvia R. B. Uliana | 2014 | BioMed Research International2014，，： | 1 |
| 16 | Ultrsound Enhanced Reactions Involving Activated Carbons As Catalysts:Synthesis of Unsaturated Nitriles显示文摘 | SILVA I F VITAL J RAMOS A M | 1998 | Carbon1998，36，： | 1 |
| 17 | Topological invariants and renormalization of Lorenz maps 显示文摘 | Silva L Ramos J S | 2002 | Physica D2002，162，34： | 1 |
| 18 | Simulation and experimental results of multiharmonic least-square fitting algorithms applied to periodic signals显示文摘 | Ramos P M Da Silva M F Martins R C | 2006 | IEEE Transactions on Instrumentation and Measurement2006，55，2： | 1 |
| 19 | Molecular typing of Mycobacterium boris isolated in the south of Brazil 显示文摘 | Ramos DF Silva AB Fagundes MQ | 2014 | Braz J Microbio12014，45，2： | 1 |
| 20 | Fresh fruits and vegetables—An overview on applied methodologies to improve its quality and safety显示文摘 | B. Ramos F.A. Miller T.R.S. Brand?o P. Teixeira C.L.M. Silva | 2013 | Innovative Food Science and Emerging Technologies2013，，： | 1 |
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