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| 1 | Agrobacterium-mediated transformation and assessment of factors influ-encing transgene expression in loblolly pine (Pinus taeda L.)显示文摘This investigation reports a protocol for transfer and expression of foreign chimeric genes in loblolly pine (Pinus taeda L.). Transformation was achieved by co-cultivation of mature zygotic embryos with Agrobacterium tumefaciens strain LBA4404 which harbored a binary vector (pBI121) including genes for β-glucuronidase (GUS) and neomycin phosphotransferase (NPTII). Factors influencing transgene expression including seed sources of loblolly pine, concentration of bacteria, and the wounding procedures of target explants were investigated. The expression of foreign gene was confirmed by the ability of mature zygotic embryos to produce calli in the presence of kanamycin, by histochemical assays of GUS activity, by PCR analysis, and by Southern blot. The successful expression of the GUS gene in different families of loblolly pine suggests that this transformation system is probably useful for the production of the genetically modified conifers. | TANG WEI North Carolina State University, 2900 Ligon Sf., Raleigh, NC 27607, USA | 2001 | Cell Research2001，11，3： | 3 |
| 2 | Characteristics of climate change in the “significant impact zone” affected by aerosols over eastern China in warm seasons显示文摘Through analysis of the distribution pattern and changing characteristics of atmospheric aerosols over the East Asia region during warm seasons in recent 20 a and beyond as well as their possible interac- tive relationship with a variety of meteorological elements, it is found that the high-value zone of aerosol optical depth derived from the Total Ozone Mapping Spectrometer (TOMS), its significant negative correlation zones in terms of sunshine duration (SD) and surface air temperature (SAT) and its significant positive correlation zones with low-level cloud amount (LCC) are co-located in the South China region during warm periods. Based on this finding, the region is referred to as a 'significant impact zone' (SI zone) affected by aerosols. Then, a comparative analysis is made on variation differ- ences of observed SAT, SD and LCC, etc. in different regions. It is also found that the LCC is increased and the SD is decreased within the 'SI zone' over eastern China during the warm season. These characteristics are more evident than those beyond the zone, while the warming trend within the zone is evidently weaker than that outside it. Studies show that since recent 20 a, under the influence of aerosols, the LCC tend to increase substantially with a clear decrease of SD and an unnoticeable warming trend within the 'SI zone'. Comparing with the climate change beyond the zone, the difference is significant. Therefore, the effects of atmospheric aerosols on climate is possibly one of the contri- butions to the difference of climate change existed between the southern and northern parts of the Eastern China during a warm season. | SHI XiaoHui1, XU XiangDe1, XIE LiAn2 1 State Key Laboratory of Severe Weather, Chinese Academy of Meteorological Sciences, Beijing 100081, China 2 Department of Marine, Earth and Atmospheric Sciences, North Carolina State University, Raleigh NC27695-8208, USA | 2008 | Science China Earth Sciences2008，51，5： | 2 |
| 3 | Expression of GP73, a resident Golgi membrane protein, in viral and nonviral liver disease显示文摘 | Raleigh D. Kladney Xiaoyen Cui Gary A. Bulla Elizabeth M. Brunt Claus J. Fimmel | 2002 | Hepatology2002，，6： | 2 |
| 4 | GP73, a novel Golgi-localized protein upregulated by viral infection显示文摘 | Raleigh D. Kladney Gary A. Bulla Linsheng Guo Andrew L. Mason Ann E. Tollefson Daniela J. Simon Zaher Koutoubi Claus J. Fimmel | 2000 | Gene2000，，1： | 2 |
| 5 | Factors influencing tissue culture responses of mature seeds and immature embryos in turf - type tall fescue显示文摘 | Raleigh | 2001 | Plant Breeding2001，120，： | 1 |
| 6 | Mortality in people with type 2 diabetes in the UK显示文摘 | Mulnier H E Seaman H E Raleigh V S | 2006 | Diabetes Med2006，23，5： | 1 |
| 7 | MINIMIZING A LINEAR FRACTIONAL FUNCTION SUBJECT TO A SYSTEM OF SUP-T EQUATIONS WITH A CONTINUOUS ARCHIMEDEAN TRIANGULAR NORM显示文摘This paper shows that the problem of minimizing a linear fractional function subject to asystem of sup-T equations with a continuous Archimedean triangular norm T can be reduced to a 0-1linear fractional optimization problem in polynomial time.Consequently,parametrization techniques,e.g.,Dinkelbach's algorithm,can be applied by solving a classical set covering problem in each iteration.Similar reduction can also be performed on the sup-T equation constrained optimization problems withan objective function being monotone in each variable separately.This method could be extended aswell to the case in which the triangular norm is non-Archimedean. | Pingke LI Edward P.Fitts Department of Industrial and Systems Engineering,North Carolina State University,Raleigh,NC 27695-7906,US Shu-Cherng FANG Edward P.Fitts Department of Industrial and Systems Engineering,North Carolina State University,Raleigh,NC 27695-7906,USA Department of Mathematical Sciences,Tsinghua University,Beijing 100084,China College of Management,Dalian University of Technology,Dalian 116024,China. | 2009 | Journal of Systems Science & Complexity2009，22，1： | 1 |
| 8 | Diabetic foot care in a long-term facili- ty显示文摘 | Berry RM Raleigh ED | 2004 | J Gerontol Nursing2004，30，4： | 1 |
| 9 | A national ra- diation oncology medical student clerkship survey: didactic curricular components increase confidence in clinical com- petency显示文摘 | Jagadeesan VS Raleigh DR Koshy M | 2014 | Int J Radiat Oncol Biol Phys2014，188，1： | 1 |
| 10 | Frictional behavior and constitutive modeling of simulated fault gouge显示文摘 | MARONE C RALEIGH C B SCHOLZ C H | 1990 | Journal of Geophysical Research (Solid Earth)1990，95，5： | 1 |
| 11 | 2011, The search for safety: The effects of conflict, poverty and ecological influences on migration in the developing world 显示文摘 | RALEIGH C | 2011 | Global environmental change-human and policy dimensions2011，21，1： | 1 |
| 12 | Diabetic foot care in a long-term facility显示文摘 | Berry RM Raleigh ED | 2004 | Gerontol Nursing2004，30，4： | 1 |
| 13 | Prediction of the Haicheng Earthquake显示文摘 | Raleigh C B G Bennett H Craig | 1977 | Eos Trans Am Geophys Union1977，72，： | 1 |
| 14 | Effects of surface perturbations on the asymmetric vortex flow over a slender body显示文摘 | Moskovitz C A Raleigh N C Hall R M | 1988 | AIAA1988，0483，： | 1 |
| 15 | The next stage in the health care economy:Aligning the interests of patients,providers,and third-party payers through consumer-driven health care plans显示文摘 | Samuel T Raleigh S Hower J | 2003 | Am J Surg2003，186，： | 1 |
| 16 | Detecting Trichodesmium blooms in SeaWiFS imagery显示文摘 | AJIT Subramaniam CHRISTOPHER W Brown RALEIGH R Hood | 2002 | Deep-Sea Resereh: II2002，49，： | 1 |
| 17 | Mortality and cancer incidence in ammonium perfluorooetanoate production workers 显示文摘 | Raleigh KK Alexander BH Olsen GW | 2014 | Oceup Environ Ned2014，71，： | 1 |
| 18 | Multivariate modulation and coding for wireless communication显示文摘 | Raleigh G G Jones V K | 1999 | IEEE J Sel Areas Comm1999，17，5： | 1 |
| 19 | Spatio-temporal coding for wireless communications 显示文摘 | Raleigh G G Cioffi J M | 1998 | IEEE Trans Wireless Commun1998，46，3： | 1 |
| 20 | Spatio-temporal coding for wireless communication显示文摘 | Raleigh G G Cioffi J M | 1998 | IEEE Transactions on Communications1998，46，3： | 1 |
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