共被期刊论文引用了3次
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| 1 | 农杆菌介导巨桉Eg5高效遗传转化显示文摘以巨桉(Eucalyptus grandis)无性系Eg5叶片为外植体,探讨了农杆菌(Agrobacterium tumefaciens)侵染时间、共培养pH值和共培养时间对瞬时转化效率的影响,分析了不同筛选策略对遗传转化植株筛选效果的影响。结果表明,外植体侵染45分钟,共培养pH值为5.8,共培养3天所得到的瞬时转化效率最高;逐步提高卡那霉素(Km)浓度筛选转基因植株有效,筛选率达到15%,转化率达到0.26%。经过GUS染色分析和PCR检测,证实为转基因植株。 | 郭利军 曾炳山 刘英 | 2013 | 植物学报2013，48，1： | 4 |
| 2 | Transient gene expression in western white pine using agroinfiltration显示文摘A method for transient gene expression was developed for western white pine(WWP,Pinus monticola Dougl.ex D.Don)using reporter gene uidA encodingβ-glucuronidase(GUS).GUS was transiently expressed in cross sections of primary and secondary needles,cotyledons,and current and second year stems of WWP via vacuum-infiltration with Agrobacterium tumefaciens.Histochemical assays of cross sections of secondary needles showed stronger blue color indicating GUS expression at day 1 and 2 than on other days post agroinfiltration(dpa).GUS activity expressed inside WWP cells was confirmed using light microscopy.In fluorometric assays,GUS expression was high at 1 dpa and lasted until 4 dpa in detached secondary needles,while similarly high expression levels only lasted until 2 dpa in attached secondary needles then dropped significantly.Although the length of GUS-staining zones varied among different WWP organs and between growth and dormant seasons,all tested WWP tissues using the protocol had high levels of transient GUS expression.Thus,heterologous candidate genes or endogenous silencing can be expressed in various WWP tissues or organs using this agroinfiltration approach.The current protocol for efficient transient gene expression will aid functional genomics study of WWP and its pathogens and related conifer species. | Zhenguo Ma Jun-Jun Liu Arezoo Zamany Holly Williams | 2020 | Journal of Forestry Research2020，31，5： | 1 |
| 3 | 农杆菌介导的针叶树种遗传转化研究进展显示文摘农杆菌介导法是目前遗传转化应用最广泛的技术手段之一。本文主要从农杆菌菌株、外植体类型、预培养时间、侵染过程、共培养条件以及抗性细胞的筛选等方面综述了影响针叶树转化效率的因素,为针叶树的遗传转化及遗传育种研究提供参考。 | 刘炎 刘克俭 王江 王颖 张毓航伊 王昊 初艳 由香玲 | 2021 | 黑龙江农业科学2021，，4： | 1 |
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