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| 1 | Translational pancreatic cancer research:a comparative study on patient-derived xenograft models显示文摘AIM To assess the viability of orthotopic and heterotopic patient-derived pancreatic cancer xenografts implanted into nude mice.METHODS This study presents a prospective experimental analytical follow-up of the development of tumours in mice upon implantation of human pancreatic adenocarcinoma samples. Specimens were obtained surgically from patients with a pathological diagnosis of pancreatic adenocarcinoma. Tumour samples from pancreatic cancer patients were transplanted into nude mice in three different locations(intraperitoneal, subcutaneous and pancreatic). Histological analysis(haematoxylin-eosin and Masson's trichrome staining) and immunohistochemical assessment of apoptosis(TUNEL), proliferation(Ki-67), angiogenesis(CD31) and fibrogenesis(α-SMA) were performed. When a tumour xenograft reached the target size, it was reimplanted in a new nude mouse. Three sequential tumour xenograft generations were generated(F1, F2 and F3).RESULTS The overall tumour engraftment rate was 61.1%. The subcutaneous model was most effective in terms of tissue growth(69.9%), followed by intraperitoneal(57.6%) and pancreatic(55%) models. Tumour development was faster in the subcutaneous model(17.7 ± 2.6 wk) compared with the pancreatic(23.1 ± 2.3 wk) and intraperitoneal(25.0 ± 2.7 wk) models(P = 0.064). There was a progressive increase in the tumour engraftment rate over successive generations for all three models(F1 28.1% vs F2 71.4% vs F3 80.9%, P < 0.001). There were no significant differences in tumour xenograft differentiation and cell proliferation between human samples and the three experimental models among the sequential generations of tumour xenografts. However, a progressive decrease in fibrosis, fibrogenesis, tumour vascularisation and apoptosis was observed in the three experimental models compared with the human samples. All three pancreatic patient-derived xenograft models presented similar histological and immunohistochemical characteristics.CONCLUSION In our experience, the faster development andgreatest number of viable xenografts could make the subcutaneous model the best option for experimentation in pancreatic cancer. | Mercedes Rubio-Manzanares Dorado Luis Miguel Marín Gómez Daniel Aparicio Sánchez Sheila Pereira Arenas Juan Manuel Praena-Fernández Juan Jose Borrero Martín Francisco Farfán López Miguel ángel Gómez Bravo Jordi Muntané Relat Javier Padillo Ruiz | 2018 | World Journal of Gastroenterology2018，24，7： | 2 |
| 2 | Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection显示文摘Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity components.Dendritic cells(DCs)play a key role in the defense against viral infections,for instance plasmacytoid DCs(pDCs),have the capacity to produce vast amounts of interferon-alpha(IFN-α).In COVID-19 there is a deficit in DC numbers and IFN-αproduction,which has been associated with disease severity.In this work,we described that in addition to the DC deficiency,several DC activation and homing markers were altered in acute COVID-19 patients,which were associated with multiple inflammatory markers.Remarkably,previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+myeloid DCs and pDCs seven months after SARS-CoV-2 infection.Moreover,the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients,while no restoration of integrinβ7 and indoleamine 2,3-dyoxigenase(IDO)levels were observed.These findings contribute to a better understanding of the immunological sequelae of COVID-19. | Alberto Pérez-Gómez Joana Vitallé Carmen Gasca-Capote Alicia Gutierrez-Valencia María Trujillo-Rodriguez Ana Serna-Gallego Esperanza Muñoz-Muela María de los Reyes Jiménez-Leon Mohamed Rafii-El-Idrissi Benhnia Inmaculada Rivas-Jeremias Cesar Sotomayor Cristina Roca-Oporto Nuria Espinosa Carmen Infante-Domínguez Juan Carlos Crespo-Rivas Alberto Fernández-Villar Alexandre Pérez-González Luis Fernando López-Cortés Eva Poveda Ezequiel Ruiz-Mateos JoséMiguel Cisneros Sonsoles Salto-Alejandre Judith Berastegui-Cabrera Pedro Camacho-Martínez Carmen Infante-Domínguez Marta Carretero-Ledesma Juan Carlos Crespo-Rivas Eduardo Márquez JoséManuel Lomas Claudio Bueno Rosario Amaya JoséAntonio Lepe Jerónimo Pachón Elisa Cordero Javier Sánchez-Céspedes Manuela Aguilar-Guisado Almudena Aguilera Clara Aguilera Teresa Aldabo-Pallas Verónica Alfaro-Lara Cristina Amodeo Javier Ampuero María Dolores Avilés Maribel Asensio Bosco Barón-Franco Lydia Barrera-Pulido Rafael Bellido-Alba Máximo Bernabeu-Wittel Candela Caballero-Eraso Macarena Cabrera Enrique Calderón Jesús Carbajal-Guerrero Manuela Cid-Cumplido Yael Corcia-Palomo Juan Delgado Antonio Domínguez-Petit Alejandro Deniz Reginal Dusseck-Brutus Ana Escoresca-Ortega Fátima Espinosa Nuria Espinosa Michelle Espinoza Carmen Ferrándiz-Millón Marta Ferrer Teresa Ferrer Ignacio Gallego-Texeira Rosa Gámez-Mancera Emilio García Horacio García-Delgado Manuel García-Gutiérrez María Luisa Gascón-Castillo Aurora González-Estrada Demetrio González Carmen Gómez-González Rocío González-León Carmen Grande-Cabrerizo Sonia Gutiérrez Carlos Hernández-Quiles Inmaculada Concepción Herrera-Melero Marta Herrero-Romero Luis Jara Carlos Jiménez-Juan Silvia Jiménez-Jorge Mercedes Jiménez-Sánchez Julia Lanseros-Tenllado Carmina López Isabel López Álvaro López-Barrios Luis F.López-Cortés Rafael Luque-Márquez Daniel Macías-García Guillermo Martín-Gutiérrez Luis Martín-Villén JoséMolina Aurora Morillo María Dolores Navarro-Amuedo Dolores Nieto-Martín Francisco Ortega María Paniagua-García Amelia Peña-Rodríguez Esther Pérez Manuel Poyato Julia Praena-Segovia Rafaela Ríos Cristina Roca-Oporto Jesús F.Rodríguez María Jesús Rodríguez-Hernández Santiago Rodríguez-Suárez Ángel Rodríguez-Villodres Nieves Romero-Rodríguez Ricardo Ruiz Zida Ruiz de Azua Celia Salamanca Sonia Sánchez Víctor Manuel Sánchez-Montagut César Sotomayor Alejandro Suárez Benjumea Javier Toral | 2021 | Cellular & Molecular Immunology2021，18，9： | 2 |
| 3 | Gastric cancer in young people under 30 years of age: worse prognosis, or delay in diagnosis?显示文摘 | Horacio N López-Basave Diaz Romero C Alejandro Padilla Rosciano Melchor Ruan J Silvio A. ?amendys-Silva Vela Sarmiento I Ruiz Molina JM Calderillo Ruiz G Flavia Morales-Vásquez Abelardo Meneses Garcia Angel Herrera-Gomez | 2013 | Cancer Management and Research (default)2013，，： | 2 |
| 4 | A computer model for teaching the dynamic behavior of AC contac- tors显示文摘 | RUIZ J R ESPINOSA A G ROMERAL L | 2010 | IEEE Transactions on Education2010，53，2： | 1 |
| 5 | Psoriatic arthritis: a clinical entity distinct from psoriasis? 显示文摘 | Ruiz D G Azevedo M N Santos O L | 2012 | Rev Bras Reumatol2012，52，4： | 1 |
| 6 | Evaluation of the antioxidant and antiproliferative potential of bioflavors显示文摘 | Bicas J L Neri-Numa I A Ruiz A L T G | 2011 | Food and Chemical Toxicology2011，49，： | 1 |
| 7 | Synthesis, structure and surface dynamics of phoshorylcholine functional biomimicking polymers显示文摘 | Ruiz L Hilbom J G Leonard D | 1998 | Biomaterials1998，19，： | 1 |
| 8 | Production of thermonuclear neutrons from deuterium-filled capsule implosions driven by Z-pinch dynamic hohlraums显示文摘 | RUIZ C L COOPER G W SLUTZ S A | 2004 | Physical Review Letters2004，93，01： | 1 |
| 9 | Evaluation of the antioxidant and antiproliferative potential of bioflavors显示文摘 | BICAS J L NERI-NUMA I A RUIZ A L T G | | 0，，： | 1 |
| 10 | The effect of a ruminal nitrogen (N) deficiency in dairy cows: evaluation of the Cornell net carbohydrate and protein system ruminal N deficiency adjustment 显示文摘 | Ruiz R Albrecht G L Tedeschi L O | 2002 | Journal of Animal Science2002，85，： | 1 |
| 11 | Vaccination for protection of retinal ganglion cells against death from glutamate cytotoxicity and ocular hypertension: Implications for glaueoma显示文摘 | Schori H Kipnis J Yoles E WoldeMussie E Ruiz G Wheeler L A and Schwartz M | 2001 | Proc Natl Aead Sci U S A2001，，98： | 1 |
| 12 | Expression and function of Oatl and Oat3 in rat kidney exposed to mercuric chloride显示文摘 | Di Giusto G Anzai N Ruiz M L | 2009 | Arch Toxicol2009，83，： | 1 |
| 13 | The injection of plasmid DNA in mouse muscle results in lifelong persistence of DNA, gene expression, and humoral response显示文摘 | Armengol G Ruiz L M Orduz S | 2004 | Mol Biotechnol2004，27，2： | 1 |
| 14 | Absolute calibration of a total yield indium activation detector for DD and DT neutrons显示文摘 | Ruiz C L Leeper R J Cooper G | 1992 | Rev Sci Instrum1992，63，10： | 1 |
| 15 | Copper activation deuterium-tritium neutron yield measurements at the National Ignition Facility 显示文摘 | Cooper G W Ruiz C L Leeper R J | 2012 | Review of Scientific Instruments2012，83，10： | 1 |
| 16 | Evaluation of the antioxidant and antiproliferative potential of bioflavors显示文摘 | Bicas J L Neri-Numa I A Ruiz A L T G | 2011 | Food and Chemical Toxicology2011，49，： | 1 |
| 17 | The effect of a ruminal nitrogen (N) deficiency in dairy cows: evaluation of the Cornell Net Carbohydrate and Protein System ruminal nitrogen deficiency adjustment显示文摘 | Ruiz R Tedeschi L O Marini J C Fox D G Pell A N Jarvis G Russell J B | 2002 | Journal of Dairy Science2002，85，： | 1 |
| 18 | Biomass and productivity of water - hyacinth and their application in control programs 显示文摘 | Gutierrez E L Ruiz E F Uribe E G | 2001 | Aquatic Plant Management2001，31，： | 1 |
| 19 | lacustrine sediments provide geochemical evidence of environmental change during the last millennium in southeastern Brazil显示文摘 | Oliveiraa S M B Saia S E M G Ruiz L C | 2009 | Chemic der Erde-Geochemistry2009，69，4： | 1 |
| 20 | Enantioselective hydrogenation of ethyl pyruvate and 1 - phenyl - 1, 2 - propanedione on catalysts prepared by impregnation of colloidal platinum on SiO2 显示文摘 | Ruiz D P Fierrob J L G Reyes P A | 2010 | J Braz C hem Soc2010，21，2： | 1 |
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