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| 1 | Ephrin-B2 is differentially expressed in the intestinal epithelium in Crohn's disease and contributes to accelerated epithelial wound healing in vitro显示文摘AIM: Eph receptor tyrosine kinases and their membrane bound receptor-like ligands, the ephrins, represent a bi-directional cell-cell contact signaling system that directs epithelial movements in development. The meaning of this system in the adult human gut is unknown. We investigated the Eph/ephrin mRNA expression in the intestinal epithelium of healthy controls and patients with inflammatory bowel disease (IBD).METHODS: mRNA expression profiles of all Eph/ephrin family members in normal small intestine and colon were established by real-time RT-PCR. In addition, differential expression in IBD was investigated by cDNA array technology, and validated by both real-time RT-PCR and immunohistochemistry. Potential effects of enhanced EphB/ephrin-B signaling were analyzed in an in vitro IEC-6 cell scratch wound model.RESULTS: Human adult intestinal mucosa exhibits a complex pattern of Eph receptors and ephrins. Beside the known prominent co-expression of EphA2 and ephrinA1,we found abundantly co-expressed EphB2 and ephrin-B1/2.Interestingly, cDNA array data, validated by real-time PCR and immunohistochemistry, showed upregulation of ephrin-B2 in both perilesional and lesional intestinal epithelial cells of IBD patients, suggesting a role in epithelial homeostasis. Stimulation of ephrin-B signaling in ephrinB1/2 expressing rat IEC-6-cells with recombinant EphB1Fc resulted in a significant dose-dependent acceleration of wound closure. Furthermore, fluorescence microscopy showed that EphB1-Fc induced coordinated migration of wound edge cells is associated with enhanced formation of lamellipodial protrusions into the wound, increased actin stress fiber assembly and production of laminin at the wound edge.CONCLUSION: EphB/ephrin-B signaling might represent a novel protective mechanism that promotes intestinal epithelial wound healing, with potential impact on epithelial restitution in IBD. | Christian Hafner Stefanie Meyer Thomas Langmann Gerd Schmitz Frauke Bataille Ilja Hagen Bernd Becker Alexander Roesch Gerhard Rogler Michael Landthaler Thomas Vogt | 2005 | World Journal of Gastroenterology2005，11，26： | 9 |
| 2 | 凝固条件和热处理及微量锶对一种Al-Si合金组织和性能的影响显示文摘研究了凝固条件和热处理及微量锶对A356合金组织与性能的影响。试验结果表明,锶的加入不仅对显微组织而且对合金中αAl固溶体的弥散析出行为也有较大的改变,加入锶后的合金在冷却过程中(“隐形热处理”)的一次弥散相和随后热处理的二次弥散相都要比A356合金细得多。这种细化作用较普通光学显微组织对力学性能的影响还要大。即使通过长时间的热处理,锶和冷却速度对显微组织的影响仍没有实质的变化。同时比较了定向凝固的A356合金在铸态与热处理态下的质量指数,结果显示其明显高于普通条件下的A356合金的质量指数。 | 徐才录 HMTensi RRoesch SSpaic 梁吉 魏秉庆 吴德海 | 1998 | 金属热处理1998，23，2： | 5 |
| 3 | Ephrin-B reverse signaling induces expression of wound healing associated genes in IEC-6 intestinal epithelial cells显示文摘AIM: Eph receptors and ephrin ligands play a pivotal role in development and tissue maintenance. Since previous data have indicated an involvement of ephrin-B2 in epithelial healing, we investigated the gene expression and downstream signaling pathways induced by ephrin-B mediated cell-cell signaling in intestinal epithelial cells.METHODS: Upon stimulation of ephrin-B pathways in IFC-6 cells with recombinant rat EphB1-Fc, gene expression was analyzed by Affymetrix(R) rat genome 230 high density arrays at different time points. Differentially expressed genes were confirmed by real-time RT-PCR. In addition, MAP kinase pathways and focal adhesion kinase (FAK) activation downstream of ephrin-B were investigated by immunoblotting and fluorescence microscopy.RESULTS: Stimulation of the ephrin-B reverse signaling pathway in IEC-6 cells induces predominant expression of genes known to be involved into wound healing/cell migration, antiapoptotic pathways, host defense and inflammation. Cox-2, c-Fos, Egr-1, Egr-2, and MCP-1 were found among the most significantly regulated genes.Furthermore, we show that the expression of repairrelated genes is also accompanied by activation of the ERK1/2 MAP kinase pathway and FAK, two key regulators of epithelial restitution.CONCLUSION: Stimulation of the ephrin-B reverse signaling pathway induces a phenotype characterized by upregulation of repair-related genes, which may partially be mediated by ERK1/2 pathways. | Christian Hafner Stefanie Meyer Ilja Hagen Bernd Becker Alexander Roesch Michael Landthaler Thomas Vogt | 2005 | World Journal of Gastroenterology2005，11，29： | 2 |
| 4 | Development and neurogenic potential of Müller glial cells in the vertebrate retina显示文摘 | Jadhav AP Roesch K Cepko CL | 2009 | Prog Retin Eye Res2009，28，4： | 1 |
| 5 | From dimming to bright-ening:Decadal changes in solar radiation at earth’s surface显示文摘 | Wild M Gilgen H Roesch A | 2005 | Science2005，308，5723： | 1 |
| 6 | 浅论YAG固体激光焊接技术在线缆行业中的应用显示文摘 | ROESCH K | 2000 | 光纤与电缆及其应用技术2000，，6： | 1 |
| 7 | Post - conditioning inhibits Mitochondrial permeability transition 显示文摘 | Argaud L Gateau - Roesch O Raisky O | 2005 | Circulation2005，111，2： | 1 |
| 8 | Transforaminal interbody fusion versus anterior - posterior interbody fusion of the lumbar spine : a financial analysis显示文摘 | Whitecloud TS 3^rd Roesch WW Ricciardi JE | 2001 | J Spinal Disord2001，14，： | 1 |
| 9 | Comparison of antibiotic resistance of udder pathogens in dairy cows kept on organic and on conventional farms 显示文摘 | ROESCH M PERRETEN V DOHERR M G | 2006 | J Dairy Sci2006，89，： | 1 |
| 10 | Sipuleucel-T and immunotherapy in the treatment of prostate cancer显示文摘 | Dawson NA Roesch EE | 2014 | Expert Opin Biol Ther2014，14，5： | 1 |
| 11 | Transforaminalinterbody fu- sion versus anterior-posterior interbody fusion of the lumbar spine: a fi- nancial analysis 显示文摘 | Whitecloud TS Roesch WW Ricciardi JE | 2001 | J Spinal Disord2001，14，2： | 1 |
| 12 | Regulation of type 1 fimbriae by unlinked FimB - and FimE - like recombinases in uropathogenic Escherichia coli strain CFT073 显示文摘 | Bryan A P Roesch L Davis | 2006 | Infect Immun2006，74，： | 1 |
| 13 | Parametricstudy of Cs/CaO sorbents with respect to simulated fluegas at high temperature 显示文摘 | Roesch A Reddy E P Smimiotis P G | 2005 | Industrial and Engineer-ing Chemistry Research2005，44，16： | 1 |
| 14 | Sex differences in adolescent depression:Stress exposure and reactivity models显示文摘 | Hankin B.L Mermelstein R Roesch L | | 0，，01： | 1 |
| 15 | From dimming to brightening: decadal changes in solar radiation at earth s surface显示文摘 | Wild M Origen H Roesch A | 2005 | Science2005，308，84： | 1 |
| 16 | Diethyl bis(hydroxymethyl)malonate显示文摘 | Roesch A | 1937 | Bull Soc Chim France1937，5，4： | 1 |
| 17 | From dimming to brightening: decadal changes in solar radiation at EarthSs surface 显示文摘 | Wild M Gilgen H Roesch A | 2005 | Science2005，308，： | 1 |
| 18 | 查看详情显示文摘 | Lu J Poppitt S.D Othman A.A Sunderland T Ruggiero K Willett M.S Diamond L.E Garcia W.D Roesch B.G Cooper GJS | | 0，，： | 1 |
| 19 | Sol-gel Pd exhaust catalysts and hydrocarbon speciation显示文摘 | Roesch S Sermon P A Wallum A | 2001 | Topics in Catalysis2001，1617，14： | 1 |
| 20 | Texture and microstructure of emulsions prepared with soy protein concentrate by high-pressure homogenization 显示文摘 | Roesch R R Corredig M | 2003 | Lebensmittel Wissenschaft trod Technologie2003，36，： | 1 |
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