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| 1 | Characterizations and identification of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping显示文摘Previous study indicated that the thermo-sensitive genic malesterile(TGMS) gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS system in two-line breeding is laborsaving,timesaving,simple,inexpensive,efficient,and eliminating the limitations of the cytoplasmic male sterility(CMS) system.'AnnongS' is the first discovered and deeply studied TGMS rice lines in China.'AnnongS-1' and 'Y58S',two derivatives of TGMS line AnnongS,were both controlled by a single recessive gene named tms5,which was genetically mapped on chromosome 2.In this study,three populations('AnnongS-1' × 'Nanjing11','Y58S' × 'Q611',and 'Y58S' × 'Guanghui122') were developed and used for the molecular fine mapping of the tms5 gene.By analyzing recombination events in the sterile individuals using a total of 125 probes covering the tms5 region,the tms5 gene was physically mapped to a 19-kb DNA fragment between two markers 4039-1 and 4039-2,which were located on the BAC clone AP004039.After the construction of the physical map between two markers 4039-1 and 4039-2,a member(ONAC023) of the NAC(NAM-ATAF-CUC-related) gene family was identified as the candidate gene of the tms5 gene. | YANG Qing-kai1,LIANG Chun-yang1,LI Jun1,JIN De-min1,AHUANG Wen2,DENG Qi-yun2,WANG Bin1(1.The State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,100101 2. Hunan Hybrid Rice Research Center,Changsha 410125) | 2007 | 湖南农业大学学报(自然科学版)2007，33，S1： | 34 |
| 2 | Dissection of genetic overlap of salt tolerance QTLs at the seedling and tillering stages using backcross introgression lines in rice显示文摘QTLs for salt-tolerance(ST)related traits at the seedling and tillering stages were identified using 99 BC2F8 introgression lines(IL)derived from a cross between IR64(indica)as a recurrent parent and Binam(japonica)from Iran as the donor parent.Thirteen QTLs affecting survival days of seedlings(SDS), score of salt toxicity of leaves(SST),shoot K + concentration(SKC)and shoot Na + concentration(SNC) at the seedling stage and 22 QTLs underlying fresh weight of shoots(FW),tiller number per plant(TN) and plant height(PH)at the tillering stage were identified.Most QTLs detected at the tillering stage showed obvious differential expression to salt stress and were classified into three types based on their differential behaviors.Type I included 11 QTLs which were expressed only under the non-stress condition.Type II included five QTLs expressed in the control and the salt stress conditions,and three of them(QPh5,QPh8 and QTn9)had similar quantity and the same direction of gene effect,suggesting their expression was less influenced by salt stress.Type III included six QTLs which were detectable only under salt stress,suggesting that these QTLs were apparently induced by the stress.Thirteen QTLs affecting trait difference or trait stability of ILs between the stress and non-stress conditions were identified and the Binam alleles at all loci except QPh4,QTn2 and QFw2a decreased trait difference.The three QTLs less influenced by the stress and 13 QTLs affecting trait stability were considered as ST QTLs which contributed to ST.Comparing the distribution of QTLs detected at the seedling and tillering stages,most(69%)of them were genetically independent.Only four were the same or adjacent regions on chromosomes 1,2,8 and 11 harboring ST QTLs detected at the two stages,suggesting that partial genetic overlap of ST across the two stages occurs.It is likely,therefore,to develop ST rice variety for both stages by pyramiding of ST QTLs of different stages or selection against the overlapping QTLs between the two stages via marker-assisted selection(MAS). | ZANG JinPing 1 ,SUN Yong 1 ,WANG Yun 1 ,YANG Jing 1 ,LI Fang 1 ,ZHOU YongLi 1 ,ZHU LingHua 1 , Reys JESSICA2,Fotokian MOHAMMADHOSEIN 2,XU JianLong 1&LI ZhiKang 1,2 1Institute of Crop Sciences/National Key Facility for Crop Gene Resources&Genetic Improvement,Chinese Academy of Agricul- tural Sciences,Beijing 100081,China 2International Rice Research Institute,DAPO Box 7777,Metro Manila,Philippines | 2008 | Science China(Life Sciences)2008，51，7： | 32 |
| 3 | Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.)显示文摘To understand the development of rice leaf blades,we identified a new rolled-leaf mutant,w32,from indica cultivar IR64 through EMS mutagenesis. The mutant showed a stable rolled-leaf phenotype throughout the life cycle. Two F2 populations were developed by crossing w32 to cultivar IR24 and PA64. Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene. To determine the location of the gene,bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7. The rolled-leaf gene,tentatively named rl11(t),is likely a new gene as no other rolled-leaf genes have been identified near the region. By developing new SSR and InDel markers,the gene was delimited to a 52 kb region near the end of the short chromosome arm. Further fine mapping and cloning of the gene are currently underway. | SHI YongFeng1,2,CHEN Jie1,LIU WenQiang1,HUANG QiNa1,2,SHEN Bo2,LEUNG Hei3 & WU JianLi1 1 Chinese National Center for Rice Improvement/National key Laboratory for Rice Biology,China National Rice Research Institute,Hangzhou 310006,China 2 College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 310016,China 3 International Rice Research Institute,Metro Manila DAPO Box 7777,Philippines | 2009 | Science China(Life Sciences)2009，52，9： | 29 |
| 4 | Using iron fertilizer to control Cd accumulation in rice plants: A new promising technology显示文摘Effects of two kinds of iron fertilizer, FeSO4 and EDTA·Na2Fe were studied on cadmium accumulation in rice plants with two rice genotypes, Zhongzao 22 and Zhongjiazao 02, with soil culture systems. The results showed that application of iron fertilizers could hardly make adverse effects on plant growth and rice grain yield. Soil application of EDTA·Na2Fe significantly reduced the Cd accumulation in rice roots, shoots and rice grain. Cd concentration in white rice of both rice genotypes in the treatment of soil application of EDTA·Na2Fe was much lower than 0.2 mg/kg, the maximal Cd permission concentra- tion in cereal crop foods in State standard. However, soil application of FeSO4 or foliar application of FeSO4 or EDTA·Na2Fe resulted in the significant increase of Cd accumulation in rice plants including rice grain compared with the control. The results also showed iron fertilizers increased the concentra- tion of iron, copper and manganese element in rice grain and also affected zinc concentration in plants. It may be a new promising way to regulate Cd accumulation in rice grain in rice production through soil application of EDTA·Na2Fe fertilizers to maintain higher content of available iron and ferrous iron in soils. | SHAO GuoSheng, CHEN MingXue, WANG DanYing, XU ChunMei, MOU RenXiang, CAO ZhaoYun & ZHANG XiuFu China National Rice Research Institute, Hangzhou 31006, China | 2008 | Science China(Life Sciences)2008，51，3： | 28 |
| 5 | Identification of quantitative trait loci associated with salt tolerance at seedling stage from Oryza rufipogon显示文摘土壤咸度是影响工厂生长和庄稼生产的主要不能生活的压力之一。在现在的学习,在米饭幼苗舞台的盐忍耐用 87 根基因渗入线(IL ) 被评估,它从在精英 indica 栽培变种 Teqing 和普通野米饭(Oryza rufipogon Griff ) 的就职之间的一个十字被导出。实质的变化为包括盐忍耐 20 的四个特点(圣) 被观察,相对的根干燥重量(RRW ) ,干燥重量(RSW ) 和相对总数弄干的相对射击重量(RTW ) 。圣断然显著地所有另外的三个特点地被相关。与这四个特点联系的 15 通常认为的量的特点 loci (QTL ) 的一个总数用单个点的分析被检测,它位于染色体 1, 2, 3, 6, 7, 9 和 10 与解释 phenotypic 变化的 8%26% 。O。在 13 QTL (86.7%) 的导出 rufipogon 的等位基因能在 Teqing 背景改进盐忍耐。影响 RRW, RSW 和 RTW 的四 QTL 簇在染色体上被发现 6, 7, 9 和 10 分别地。在这四 QTL 簇之中,包括三 QTL (qRRW10, qRSW10 和 qRTW10 ) 的主要的簇在染色体 10 的长手臂上在制造者 RM271 附近被发现,并且 O。分别地,当 phenotypic 变化由这解释为三个特点的三单个 QTL 从 19% ~ 26% 变化了时,在这三 loci 的导出 rufipogon 的等位基因与 22.7% , 17.3% 和 18.5% 的添加剂效果增加了 RRW, RSW 和 RTW。另外,几腌容忍的 IL 被选择并且能被用于识别并且利用有利的盐从普通野米饭的容忍的基因并且在盐使用容忍的米饭繁殖编程序。 | Lei Tian,Lubin Tan,Fengxia Liu,Hongwei Cai,Chuanqing Sun State Key Laboratory of Plant Physiology and Biochemistry,National Centre for Evaluation of Agricultural Wild Plant(Rice), Laboratory of Crop Heterosis and Utilization of Ministry of Education Beijing Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture,Department of Plant Genetics and Breeding,China Agricultural University,Beijing 100193,China | 2011 | Journal of Genetics and Genomics2011，38，12： | 26 |
| 6 | 在血压控制不良的高血压患者中使用数字化干预的家庭和在线血压管理:随机对照试验显示文摘HOME BP试验(The home and online management and evaluation of blood pressure)的目的是评估在初级预防中结合自我监测和自我管理的数字化干预在高血压管理中的作用。研究者纳入英国76个治疗中心经过治疗但血压控制不好(>140/90 mm Hg, 1 mm Hg=0.133 kPa)并能上网的患者622例,进行自动确定主要终点的公开随机对照试验。 | McMa-nus RJ Little P Stuart B Morton K Raftery J Kelly J Bradbury K Zhang J Zhu S Murray E May CR Mair FS Michie S Smith P Band R Ogburn E AllenJ Rice C Nut-tall J Williams B Yardley L 陈嘉睿(译) 叶鹏(审校) | 2021 | 中华高血压杂志2021，29，5： | 15 |
| 7 | Epithelial ovarian cancer:An overview显示文摘Ovarian cancer is the second most common gyneco-logical cancer and the leading cause of death in the United States. In this article we review the diagnosis and current management of epithelial ovarian cancer which accounts for over 95 percent of the ovarian malignancies. We will present various theories about the potential origin of ovarian malignancies. We will discuss the genetic anomalies and syndromes that may cause ovarian cancers with emphasis on Breast cancer type 1/2 mutations. The pathology and pathogenesis of ovarian carcinoma will also be presented. Lastly, we provide a comprehensive overview of treatment strategies and staging of ovarian cancer, conclusions and future directions. | Arpita Desai Jingyao Xu Kartik Aysola Yunlong Qin Chika Okoli Ravipati Hariprasad Ugorji Chinemerem Candace Gates Avinash Reddy Omar Danner Geary Franklin Anachebe Ngozi Guilherme Cantuaria Karan Singh William Grizzle Charles Landen Edward E Partridge Valerie Montgomery Rice E Shyam P Reddy Veena N Rao | 2014 | World Journal of Translational Medicine2014，3，1： | 10 |
| 8 | Morphology and mapping analysis of rice(Oryza sativa L.)clustered spikelets(Cl)mutant显示文摘The rice clustered spikelets (Cl) mutant exhib-its a phenotype that most of branch apical have 2-3 spikelets clustered together. SEM (scanning electron microscope) ob-servation suggested that the Cl gene controlled branch apical development, and influenced the terminal spikelets elonga-tion. The spikelet number was reduced in mutant, indicating that Cl may also have an effect on spikelet number. To map Cl locus, two F2 mapping populations derived from the crosses between the Cl and ZhongHua11, and Cl and ZheFu802 were constructed, respectively. The Cl locus was roughly mapped between two CAPS markers, CK0214 and SS0324. A further fine mapping analysis showed that the Cl locus was mapped between makers R0674E and Cl2560, with genetic distances of 0.2 and 2.1 cM, respectively. Then we found a PAC contig spanning Cl locus, the region was delim-ited to 196 kb. This result was useful for cloning of the Cl gene. Allelism test demonstrated that Cl was allelic to Cl2, another rice clustered spikelets mutant. | ZHENG Leiying1, ZHU Xudong2, QIAN Qian2, ZHAO Zhong1, ZHANG Jianjun3, HU Xiaohe1, LIN Hongxuan1,4 & LUO Da1 1. National Key Laboratory of Plant Molecular Genetics, Shanghai In-stitute of Plant Physiology and Ecology, Chinese Academy of Sci-ences, Shanghai 200032, China 2. China National Rice Research Institute, Hangzhou 310006, China 3. Crop Institute of Breeding and Cultivation, Shanghai Agricultural Academy, Shanghai 201106, China 4. Shanghai Anxin Research Foundation Laboratory, Shanghai 200032, China | 2003 | Chinese Science Bulletin2003，48，6： | 10 |
| 9 | 小胶质细胞再激活可缓解脑损伤后炎症反应并促进脑功能恢复显示文摘中枢神经系统疾病或损伤后,小胶质细胞介导的慢性神经炎症反应会损伤局部脑组织,加重病情,不利于神经功能的恢复。小胶质细胞的激活通过集落刺激因子1受体(CSF1R)信号通路,CSF1R抑制剂可阻断小胶质细胞的激活。抑制慢性神经炎症反应是治疗中枢神经系统疾病的有效方法,但长时间抑制小胶质细胞在临床实践中无法实现。值得注意的是,去掉CSF1R抑制剂可刺激中枢神经系统内新的小胶质细胞的彻底重激活。本课题组建立小鼠广泛性神经元丢失模型,在该模型中探讨急性小胶质细胞的消除及再激活的作用。神经元的丢失导致了较长时间的神经炎性反应,表现为小胶质细胞肿胀,并表达CD68和CD45;同时,细胞因子、趋化因子、补体及其他炎症信号的表达增加。小胶质细胞的再激活可以缓解上述炎症反应,还可以促进小鼠神经功能的恢复,即使该小鼠的海马神经元丢失80%,其在水迷宫测试中的得分与正常小鼠的得分相差无几。对突触的分析结果显示,小胶质细胞的再激活可增加PSD95和突触泡蛋白点状突触体的表达,提示小胶质细胞有助于重塑和调节突触的形态和功能。综上所述,本研究结果显示,小胶质细胞的短时间消除及再激活可能是一种新颖有效的方法用于减少神经炎性反应并促进脑功能恢复。 | Rice RA Pham J Lee RJ Najafi AR West BL Green KN 唐颖馨(编译) | 2017 | 神经损伤与功能重建2017，12，3： | 9 |
| 10 | Hybrid rice technology for food security in the world显示文摘 | YUAN Long-ping(China National Hybrid Rice R & D Center,Changsha,Hunan,410125,China) | 2004 | 作物研究2004，18，4： | 9 |
| 11 | The ploidy effects in plant gene expression: Progress, problems and prospects显示文摘Polyploidy and haploid are widely employed in the studies of genetics and evolution, and great pro-gress has been made in these fields, inspiring the enthusiasm of scientists to explore the ploidy effects in gene expression. In this paper, we review the gene expression and its regulation in polyploids, es-pecially in autopolyploids. We summarize some limitations in previous reports on polyploidy gene ex-pression and its regulation, especially the limitations in the research materials. We propose an idea to create homologous ploidy series with twin-seedlings and to employ high-throughput techniques to investigate the polyploidy transcriptome and its regulation. | PENG Hai1, ZHANG Jing1 & WU XianJun2,3 1 Laboratory of Germplasm and Genetics, College of Life Science, Jianghan University, Wuhan 430056, China 2 Rice Research Institute of Sichuan Agricultural University, Chengdu 611130, China 3 Key Laboratory of Southwest Crop Genetic Resources and Improvement of the Ministry of Education, Sichuan Agricultural Univer-sity, Ya‘an 620514, China | 2008 | Science China(Life Sciences)2008，51，4： | 8 |
| 12 | Operative complications and economic outcomes of cholecystectomy for acute cholecystitis显示文摘BACKGROUND Recent management of acute cholecystitis favors same admission(SA)or emergent cholecystectomy based on overall shorter hospital stay and therefore cost savings.We adopted the practice of SA cholecystectomy for the treatment of acute cholecystitis at our tertiary care center and wanted to evaluate the economic benefit of this practice.We hypothesized that the existence of complications,particularly among patients with a higher degree of disease severity,during SA cholecystectomy could negate the cost savings.AIM To compare complication rates and hospital costs between SA vs delayed cholecystectomy among patients admitted emergently for acute cholecystitis.METHODS Under an IRB-approved protocol,complications and charges for were obtained for SA,later after conservative management(Delayed),or elective cholecystectomies over an 8.5-year period.Patients were identified using the acute care surgery registry and billing database.Data was retrieved via EMR,operative logs,and Revenue Cycle Operations.The severity of acute cholecystitis was graded according to the Tokyo Guidelines.TG18 categorizes acute cholecystitis by Grades 1,2,and 3 representing mild,moderate,and severe,respectively.Comparisons were analyzed withχ2,Fisher’s exact test,ANOVA,ttests,and logistic regression;significance was set at P<0.05.RESULTS Four hundred eighty-six(87.7%)underwent a SA while 68 patients(12.3%)received Delayed cholecystectomy.Complication rates were increased after SA compared to Delayed cholecystectomy(18.5%vs 4.4%,P=0.004).The complication rates of patients undergoing delayed cholecystectomy was similar to the rate for elective cholecystectomy(7.4%,P=0.35).Mortality rates were 0.6%vs 0%for SA vs Delayed.Patients with moderate disease(Tokyo 2)suffered more complications among SA while none who were delayed experienced a complication(16.1%vs 0.0%,P<0.001).Total hospital charges for SA cholecystectomy were increased compared to a Delayed approach($44500±$59000 vs$35300±$16700,P=0.019).The relative risk of developing a complication was 4.2x[95%confidence interval(CI):1.4-12.9]in the SA vs Delayed groups.Among eight patients(95%CI:5.0-12.3)with acute cholecystitis undergoing SA cholecystectomy,one patient will suffer a complication.CONCLUSION Patients with Tokyo Grade 2 acute cholecystitis had more complications and increased hospital charges when undergoing SA cholecystectomy.This data supports a selective approach to SA cholecystectomy for acute cholecystitis. | Christopher P Rice Krishnamurthy B Vaishnavi Celia Chao Daniel Jupiter August B Schaeffer Whitney R Jenson Lance W Griffin William J Mileski | 2019 | World Journal of Gastroenterology2019，25，48： | 8 |
| 13 | Genetical and Physiological Basis of Plant Type Model of Erect and Large Panicle Japonica Super Rice in Northern China显示文摘The historical changes in rice yields across China were explored. The physiological mechanisms and genetic basis of the erect and large panicle super-high-yield plant type model for breeding japonica super rice were analyzed mainly on the panicle type, number of large vascular bundles (LVB) in the panicle neck, and the panicle type index (PTI). In the production point of view, we suggested that, for the breeding of super-high-yield japonica rice, the erect panicle types with more LVB numbers in the panicle neck and superior upper grains in the secondary branches would be the key factors. The information has potential significance in the rice breeding and productivity not only in China but also throughout the rice production areas of the world. | XU Zheng-jin, CHEN Wen-fu, HUANG Rui-dong, ZHANG Wen-zhong, MA Dian-rong, WANG Jia-yu, XU Hai and ZHAO Ming-hui Key Lab of Northern Japonica Rice Breeding of Liaoning Province, Shenyang Agricultural University, Shenyang 110161, P.R.China | 2010 | Agricultural Sciences in China2010，9，4： | 7 |
| 14 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 15 | SAR-3——a resource of diploid true breeding hybrid rice显示文摘In 1988, we found strain 9003 from composite hybridization of iad/ca and japonica. 9003 possesses characteristics of twin seedlings with frequency of 15-20% of the investigated popu-lation. The highest frequency was 45. 68%.Afterwards, we discovered that some twin seedlings had variabilities in chromosome set. | ZHOU Kaida, WANG Xudong, Wu Xianjun, YAN Zhibing. and ZHOU Xaohe, Rice Res Inst of Sichuan AgriUniv , Wenjiangg 611130, China | 1997 | Chinese Rice Research Newsletter1997，5，4： | 6 |
| 16 | Enhancing disease resistances of Super Hybrid Rice with four antifungal genes显示文摘A plant expression vector harboring four antifungal genes was delivered into the embryogenic calli of ‘9311’, an indica restorer line of Super Hybrid Rice, via modified biolistic particle bombardment. Southern blot analysis indicated that in the regenerated hygromycin-resistant plants, all the four anti-fungal genes, including RCH10, RAC22, β-Glu and B-RIP, were integrated into the genome of ‘9311’, co-transmitted altogether with the marker gene hpt in a Mendelian pattern. Some transgenic R1 and R2 progenies, with all transgenes displaying a normal expression level in the Northern blot analysis, showed high resistance to Magnaporthe grisea when tested in the typical blast nurseries located in Yanxi and Sanya respectively. Furthermore, transgenic F1 plants, resulting from a cross of R2 homo-zygous lines with high resistance to rice blast with the non-transgenic male sterile line Peiai 64S, showed not only high resistance to M. grisea but also enhanced resistance to rice false smut (a disease caused by Ustilaginoidea virens) and rice kernel smut (another disease caused by Tilletia barclayana). | ZHU HuaChen1, XU XinPing1, XIAO GuoYing2,3, YUAN LongPing2 & LI BaoJian1 1 Biotechnology Research Center, Key Laboratory of Gene Engineering of Ministry of Education, Sun Yat-sen University, Guangzhou 510275, China 2 China National Hybrid Rice R&D Center, Hunan Hybrid Rice Research Center, Changsha 410125, China 3 Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha 410125, China | 2007 | Science China(Life Sciences)2007，50，1： | 6 |
| 17 | Genetic analysis and molecular mapping of a novel gene for zebra mutation in rice (Oryza sativa L.)显示文摘A novel zebra mutant,zebra-15,derived from the restorer line Jinhui10(Oryza sativa L. ssp. indica) treated by EMS,displayed a distinctive zebra leaf from seedling stage to jointing stage. Its chlorophyll content decreased(55.4%) and the ratio of Chla/Chlb increased(90.2%) significantly in the yellow part of the zebra-15,compared with the wild type. Net photosynthetic rate and fluorescence kinetic parameters showed that the decrease of chlorophyll content significantly influenced the photosynthetic efficiency of the mutant. Genetic analysis of F2 segregation populations derived from the cross of Xinong1A and zebra-15 indicated that the zebra leaf trait is controlled by a single recessive nuclear gene. Ninety-eight out of four hundred and eighty pairs of SSR markers showed the diversity between the Xinong1A and the zebra-15,their F2 population was then used for gene mapping. Zebra-15(Z-15) gene was primarily restricted on the short arm of chromosome 5 by 150 F2 recessive individuals,19.6 cM from marker RM3322 and 6.0 cM from marker RM6082. Thirty-six SSR markers were newly designed in the restricted location,and the Z-15 was finally located between markers nSSR516 and nSSR502 with the physical region 258 kb by using 1,054 F2 recessive individuals. | Qiushi Wang,Xianchun Sang,Yinghua Ling,Fangming Zhao,Zhenglin Yang,Yunfeng Li,Guanghua He Key Laboratory of Biotechnology and Crop Quality Improvement,Ministry of Agriculture Rice Research Institute,Southwest University,Chongqing 400716,China | 2009 | Journal of Genetics and Genomics2009，36，11： | 5 |
| 18 | Expanded classification of hepatitis C virus into 7 genotypes and 67 subtypes: Updated criteria and genotype assignment web resource显示文摘 | Donald B. Smith Jens Bukh Carla Kuiken A. Scott Muerhoff Charles M. Rice Jack T. Stapleton Peter Simmonds | 2014 | Hepatology2014，，1： | 5 |
| 19 | Geographic Distribution of Mating Types in Magnaporthe grisea and the Relationship Between Fertile Isolates in China显示文摘377 isolates of Magnaporthe grisea were collected from 17 provinces in China and their geographic distribution of mating types and their fertility was tested with four standard isolates, KA3 and TH12 (Mat1.1) and Guy11 and TH16 (Mat1.2) provided by CIRAD. 73 fertile isolates were tested with SCAR markers of 13 pairs of primers. Preliminary results showed that the geographic distribution of M. grisea existed among isolates collected from the same location as well as different locations and the genetic relationship between fertile isolates of the fungus in China. The existence of sexual reproduction of M. grisea was explored in the field as well. | SHEN Ying, Notteghem Jean Loup., Milazzo Joelle., YUAN Xiao-ping, Adreit Henry, ZHAO Xin-hua, WANG Yan-li and Tharreau Didier.(China National Rice Research Institute, Hangzhou 310006, P. R. China National Institute of Agronomic Research ,Unit of Training and Biological Research , Ecology and Plant Pathology, 2 Place Viala , 34062 Montpellier , France International Center for collaboration in Agronomic Research and Development,TA 73 /09 , 34398 Montpellier , Cedex 05 , France) | 2002 | Agricultural Sciences in China2002，1，6： | 5 |
| 20 | 7th Edition of the AJCC Cancer Staging Manual: Esophagus and Esophagogastric Junction显示文摘 | Thomas W. Rice MD Eugene H. Blackstone MD Valerie W. Rusch MD | 2010 | Annals of Surgical Oncology2010，，7： | 4 |
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