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| 1 | 酱油掺假检测技术的研究进展显示文摘酱油掺假使杂现象已成为影响酱油行业健康发展的制约因素之一。鉴伪技术的发展对酱油质量与安全控制至关重要。分析了掺假使杂酱油引起食品不安全的原因,综述了国内外关于掺假酱油鉴伪技术的研究进展,分析其优势和不足,提出加强生物检测的建议,为该领域的研究工作进一步开展提供参考。 | 徐伟丽 单毓娟 张兰威 Pushparajah Thavarajah | 2013 | 中国调味品2013,38,11: | 5 |
| 2 | Development of a panel of unigene-derived polymorphic EST–SSR markers in lentil using public database information显示文摘Lentil(Lens culinaris Medik.), a diploid(2n = 14) with a genome size greater than 4000 Mbp, is an important cool season food legume grown worldwide. The availability of genomic resources is limited in this crop species. The objective of this study was to develop polymorphic markers in lentil using publicly available curated expressed sequence tag information(ESTs). In this study, 9513 ESTs were downloaded from the National Center for Biotechnology Information(NCBI) database to develop unigene-based simple sequence repeat(SSR) markers. The ESTs were assembled into 4053 unigenes and then analyzed to identify 374 SSRs using the MISA microsatellite identification tool. Among the 374 SSRs, 26 compound SSRs were observed.Primer pairs for these SSRs were designed using Primer3 version 1.14. To classify the functional annotation of ESTs and EST–SSRs, BLASTx searches(using E-value 1 × 10-5) against the public UniP rot(http://gffzzc0d2db89b939488bhq6q9bo6w56x56wux.ffgz.tsg.suse.edu.cn/) and NCBI(http://gffzz5b533c64939a43b3hq6q9bo6w56x56wux.ffgz.tsg.suse.edu.cn/) databases were performed. Further functional annotation was performed using PLAZA(version3.0) comparative genomics and GO annotation was summarized using the Plant GO slim category. Among the synthesized 312 primers, 219 successfully amplified Lens DNA. A diverse panel of 24 Lens genotypes was used to identify polymorphic markers. A polymorphic set of 57 markers successfully discriminated the test genotypes. This set of polymorphic markers with functional annotation data could be used as molecular tools in lentil breeding. | Debjyoti Sen Gupta Peng Cheng Gaurav Sablok Dil Thavarajah Pushparajah Thavarajah Clarice J.Coyne Shiv Kumar Michael Baum Rebecca J.McGee | 2016 | The Crop Journal2016,4,5: | 2 |
| 3 | γ-生育三烯酚对体外培养的人结肠癌HT-29细胞生长的抑制作用显示文摘为了探讨γ-生育三烯酚对体外培养的人结肠癌HT-29细胞的生长抑制作用,采用MTT、分裂指数、集落形成、形态学观察法测定细胞活力,Hoechst 33258荧光染色法和DNA ladder法检测检测细胞凋亡。结果表明:γ-生育三烯酚能够显著抑制HT-29细胞的增殖(P<0.05),且存在剂量-效应关系,其48 h半数抑制浓度(IC 50)为35.45μmol/L;形态学观察和DNA ladder结果显示受试物能够诱导HT-29细胞凋亡。γ-生育三烯酚对人结肠癌HT-29细胞具有生长抑制作用,此作用可能与其凋亡诱导效应有关。 | 徐伟丽 张兰威 杨鑫 单毓娟 Pushparajah Thavarajah 宋微 戴绍春 | 2014 | 中国粮油学报2014,29,1: | 2 |
| 4 | Molecular identification of sphaceloma perseae (avo- cado scab) and its absence in new zealand显示文摘 | Everett K R Rees-George J Pushparajah I P S | 2011 | Journal of Phytopathology2011,159,2: | 1 |
| 5 | Novel starch based nano scale enteric coatings from soybean meal for co- lon-specific delivery显示文摘 | NILUSHNI S PUSHPARAJAH T JAE-BOM O | 2014 | Carbohydr Polym2014,111,: | 1 |
| 6 | Nutrition and fertil- izer use in Hevea and associated cov- ers in Peninsualar Malaysia-a review 显示文摘 | PUSHPARAJAH E | 1977 | J Rubb Res Inst Sri Lanka1977,54,: | 1 |
| 7 | Sustainable Land Management显示文摘 | Pushparajah E | 1997 | Plant- er1997,73,1: | 1 |
| 8 | A systematic three- dimensional echocardiographic approach to assist surgical planning in double outlet right ventricle显示文摘 | Pushparajah K Barlow A Tran VH | 2013 | Echocardiography2013,30,2: | 1 |
| 9 | Sustainable land management显示文摘 | Pushparajah E | 1997 | Planter1997,73,1: | 1 |
| 10 | Limitations of current treatments for systemic lupus erythematosus:a patient and physician survey显示文摘 | Strand V Galateanu C Pushparajah DS | | 0,,: | 1 |
| 11 | Sustainable land management 显示文摘 | PUSHPARAJAH E | 1997 | Planter1997,73,1: | 1 |
| 12 | Sustainable land management 显示文摘 | PUSHPARAJAH E | 1997 | Planter1997,73,1: | 1 |
| 13 | Adulteration of apple with pear juice: emphasis on major carbohydrates, proline, and arbutin 显示文摘 | Pushparajah T Nicholas H Low | 2006 | J Agric Food Chem2006,54,13: | 1 |
| 14 | Sustainable land mangement 显示文摘 | Pushparajah E | 1997 | Planter1997,73,1: | 1 |
| 15 | Sustainable Land Management 显示文摘 | Pushparajah E | 1997 | Planter1997,73,1: | 1 |
| 16 | Sustainable land mangement显示文摘 | Pushparajah E | 1997 | Planter1997,73,10: | 1 |
| 17 | Indicators and thresholds for the evaluation of sustainsble land management 显示文摘 | Syers J K Hambin A Pushparajah E | 1995 | Canadian Journal of Soil Science1995,75,4: | 1 |
| 18 | Three-dimensional fetalechocardiography for prediction of postnatal surgical approach indouble outlet right ventricle : A pilot study 显示文摘 | ZidereV Pushparajah K Allan L D al | 2013 | Ultrasound in Obs-tetrics &Gynecology2013,42,42: | 1 |
| 19 | Sustainable land management显示文摘 | Pushparajah E | 1997 | Planter1997,73,1: | 1 |
| 20 | The substantial burden of systemic lupus erythematosus on the productivity and careers of patients: a European patient-driven online survey显示文摘 | Caroline Gordon David Isenberg Kirsten Lerstr?m Yvonne Norton Enkeleida Nika? Daphnee S. Pushparajah Matthias Schneider | 2013 | Rheumatology2013,,12: | 1 |