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| 1 | pRB expression in esophageal mucosa of individuals at high risk for squamous cell carcinoma of the esophagus显示文摘AIM: To investigate the pRb expression in a large group of patients with history of chronic exposure to the main risk factors for development of squamous cell carcinoma of the esophagus. METHODS: One hundred and seventy asymptomatic individuals at high risk for esophageal squamous cell carcinoma (consumption of more than 80 g of ethanol and 10 cigarettes/d for at least 10 years) underwent upper gastrointestinal endoscopy with biopsies of the esophageal mucosa. As a control group, specimens of esophageal mucosa obtained from 20 healthy subjects were also studied. Immunohistochemical assessment of the tissues was performed using a monoclonal antibody anti-pRB protein. RESULTS: Absence of the pRB staining, indicating loss of RB function, was observed in 33 (19.4%) of the individuals at risk for esophageal cancer, but in none of the healthy controls (P < 0.02). Loss of pRb expression increased in a stepwise fashion according to the severity of the histological findings (P < 0.005): normal mucosa (11/97 or 11.3%), chronic esophagitis (17/60 or 28.3%), low-grade dysplasia (3/10 or 30%), high-grade dysplasia 1/2 or 50%) and squamous cell carcinoma (1/1 or 100%). CONCLUSION: Our findings suggest that abnormal expression of the pRB protein may be implicated in the process of esophageal carcinogenesis. Additional studies are warranted to define the role of the pRBprotein as a biomarker for development of esophageal squamous cell carcinoma in individuals at high risk for this malignancy. | Simone S Contu Paulo C Contu Daniel C Damin Renato B Fagundes Fabiano Bevilacqua Aline S Rosa Joo C Prolla Luis F Moreira | 2007 | World Journal of Gastroenterology2007，13，11： | 5 |
| 2 | No evidence of HPV DNA in esophageal squamous cell carcinoma in a population of Southern Brazil显示文摘AIM:To investigate the association between human papillomavirus(HPV)and esophageal squamous cell carcinoma(ESCC)in southern Brazil.METHODS:We studied 189 esophageal samples from125 patients from three different groups:(1)102 biopsies from 51 patients with ESCC,with one sample from the tumor and another from normal esophageal mucosa distant from the tumor;(2)50 esophageal biopsies from 37 patients with a previous diagnosis of head and neck squamous cell carcinoma(HNSCC);and(3)37 biopsies from esophageal mucosa with normal appearance from 37 dyspeptic patients,not exposed to smoking or alcohol consumption.Nested-polymerase chain reaction(PCR)with the MY09/11 and GP5/6 L1primers was used to detect HPV L1 in samples fixed in formalin and stored in paraffin blocks.All PCR reactions were performed with a positive control(cervicovaginal samples),with a negative control(Human Genomic DNA)and with a blank reaction containing all reagents except DNA.We took extreme care to prevent DNA contamination in sample collection,processing,and testing.RESULTS:The histological biopsies confirmed the diagnosis of ESCC in 52 samples(51 from ESCC group and 1 from the HNSCC group)and classified as well differentiated(12/52,23.1%),moderately differentiated(27/52,51.9%)or poorly differentiated(7/52,13.5%).One hundred twenty-eight esophageal biopsies were considered normal(51 from the ESCC group,42 from the HNSCC group and 35 from dyspeptic patients).Nine had esophagitis(7 from the HNSCC and 2 from dyspeptic patients).Of a total of 189 samples,only 6 samples had insufficient material for PCR analysis:1 from mucosa distant from the tumor in a patient with ESCC,3from patients with HNSCC and 2 from patients without cancer.In 183 samples(96.8%)GAPDH,G3PDH and/orβ-globin were amplified,thus indicating the adequacy of the DNA in those samples.HPV DNA was negative in all the 183 samples tested:52 with ESCC,9 with esophagitis and 122 with normal esophageal mucosa.CONCLUSION:There was no evidence of HPV infection in different ESCC from southern Brazil. | Luís Carlos Moreira Antunes Joo Carlos Prolla Antonio de Barros Lopes Marta Pires da Rocha Renato Borges Fagundes | 2013 | World Journal of Gastroenterology2013，19，39： | 4 |
| 3 | Identification of patients at-risk for Lynch syndrome in a hospital-based colorectal surgery clinic显示文摘AIM:To determine the prevalence of a family history suggestive of Lynch syndrome (LS) among patients with colorectal cancer (CRC) followed in a coloproctology outpatient clinic in Southern Brazil.METHODS:A consecutive sample of patients with CRC were interviewed regarding personal and family histories of cancer.Clinical data and pathology features of the tumor were obtained from chart review.RESULTS:Of the 212 CRC patients recruited,61 (29%) reported a family history of CRC,45 (21.2%) were diagnosed under age 50 years and 11 (5.2%) had more than one primary CRC.Family histories consistent with Amsterdam and revised Bethesda criteria for LS were identified in 22 (10.4%) and 100 (47.2%) patients,respectively.Twenty percent of the colorectal tumors had features of the high microsatellite instability phenotype,which was associated with younger age at CRC diagnosis and with Bethesda criteria (P < 0.001).Only 5.3% of the patients above age 50 years had been previously submitted for CRC screening and only 4% of patients with suspected LS were referred for genetic risk assessment.CONCLUSION:A significant proportion of patients with CRC were at high risk for LS.Education and training of health care professionals are essential to ensure proper management. | Patrícia Koehler-Santos Patricia Izetti Jamile Abud Carlos Eduardo Pitroski Silvia Liliana Cossio Suzi Alves Camey Cláudio Tarta Daniel C Damin Paulo Carvalho Contu Mario Antonello Rosito Patricia Ashton-Prolla Joāo Carlos Prolla | 2011 | World Journal of Gastroenterology2011，17，6： | 2 |
| 4 | Mitochondrial oxidative damage andapoptosis in age - related hearing loss 显示文摘 | Someya S Prolla TA | 2010 | Mech AgeingDev2010，131，： | 1 |
| 5 | CD30 in normal and neoplastic cells显示文摘 | CHIARLE R PODDA A PROLLA G | 1999 | Clin Immunol1999，90，2： | 1 |
| 6 | CD30 over express ion enhances negative selection in the thymus and mediates programmmed cell death via a Bcl-2 sensitive pathway显示文摘 | Chiarle R Podda A Prolla G | | 0，，01： | 1 |
| 7 | Gene expression profiling of low selenium status in the mouse intestine:Transcriptional activation of genes linked to DNA damage,cell cycle control and oxidative stress显示文摘 | Rao L Puschner B A Prolla T | 2001 | J Nutr2001，131，： | 1 |
| 8 | Angiogenesis in non- small cell lung cancer: microvessel area in needle biopsy in vascular tumor density 显示文摘 | Irion LC Prolla JC Hartmann AA | 2008 | Anal Quant Cytol Histol2008，30，2： | 1 |
| 9 | Gene-expression profile of the ageing brain in mice显示文摘 | Lee CK Weindruch R Prolla TA | 2000 | Nat Genet2000，25，3： | 1 |
| 10 | Genes encoding mitochondrial respiratory chain components are profoundly down-regulated with aging in the cochlea of DBA/2J mice显示文摘 | Shinichi Someya Tatsuya Yamasoba Tomas A. Prolla Masaru Tanokura | 2007 | Brain Research2007，，： | 1 |
| 11 | Gene Expression Profiling of Low Selenium Status in the Mouse Intestine :Transcriptional Activation of Genes Linked to DNA Damage, Cell Cycle Control and Oxidative Stress显示文摘 | Rao L Puschner B Prolla T A | 2001 | J Nutr2001，131，： | 1 |
| 12 | Destabilization of Tracts of Simple Repetitive DNA in Yeast by Mutations Affecting DNA Mismatch Repair显示文摘 | STRAND M PROLLA T A LISKAY R M | 1993 | Nature1993，365，： | 1 |
| 13 | Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1,two homologs of the bacterial mutL gene显示文摘 | Prolla TA Christie DM Liskay RM | 1994 | Mol Cell Biol1994，，2： | 1 |
| 14 | Role of mitochondrial dysfunction and mitochondrial DNA mutations in age-related hearing loss显示文摘 | Tatsuya Yamasoba Shinichi Someya Chikako Yamada Richard Weindruch Tomas A. Prolla Masaru Tanokura | 2006 | Hearing Research2006，，1： | 1 |
| 15 | DNA microarray analysis of the aging brain 显示文摘 | Prolla TA | 2002 | Chem Senses2002，27，3： | 1 |
| 16 | PGC-lct in aging and anti-aging inter- ventions 显示文摘 | Anderson R Prolla T | 2009 | Biochim Biophys Acta2009，1790，10： | 1 |
| 17 | Gene-expression profile of the ageing brain in mice显示文摘 | Lee CK Weindruch R Prolla TA | 2000 | Nat Genet2000，25，3： | 1 |
| 18 | MLH1, PMS1, and MSH2 interaction of DNA mismatch repair in yeast显示文摘 | Prolla TA Pang Q Alani E | 1994 | Science1994，265，5175： | 1 |
| 19 | SIRT4 Inhibits Glutamate Dehydrogenase and Opposes the Effects of Calorie Restriction in Pancreatic β Cells显示文摘 | Marcia C. Haigis Raul Mostoslavsky Kevin M. Haigis Kamau Fahie Danos C. Christodoulou Andrew J. Murphy David M. Valenzuela George D. Yancopoulos Margaret Karow Gil Blander Cynthia Wolberger Tomas A. Prolla Richard Weindruch Frederick W. Alt Leonard Guaren | 2006 | Cell2006，，5： | 1 |
| 20 | NAD Deficiency in age-related mitochon- drial dysfunction 显示文摘 | Prolla TA Denu JM | 2014 | Cell Metab2014，19，2： | 1 |
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