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| 1 | Validation of SNP markers for fruit quality and disease resistance loci in apple (Malus × domestica Borkh.) using the OpenArray® platform显示文摘Genome mapping has promised much to tree fruit breeding during the last 10 years.Nevertheless,one of the greatest challenges remaining to tree fruit geneticists is the translation of trait loci and whole genome sequences into diagnostic genetic markers that are efficient and cost-effective for use by breeders,who must select genetically optimal parents and subsequently select genetically superior individuals among their progeny.To take this translational step,we designed the apple International RosBREED SNP Consortium OpenArray v1.0(IRSCOA v1.0)assay using a set of 128 apple single nucleotide polymorphisms(SNPs)linked to fruit quality and pest and disease resistance trait loci.The Thermo Fisher Scientific OpenArray®technology enables multiplexed screening of SNP markers using a real-time PCR instrument with fluorescent probe-based Taqman®assays.We validated the apple IRSCOA v1.0 multitrait assay by screening 240 phenotyped individuals from the Plant&Food Research apple cultivar breeding programme.This set of individuals comprised commercial and heritage cultivars,elite selections,and families segregating for traits of importance to breeders.In total,33 SNP markers of the IRSCOA v1.0 were validated for use in marker-assisted selection(MAS)for the scab resistances Rvi2/Vh2,Rvi4/Vh4,Rvi6/Vf,fire blight resistance MR5/RLP1,powdery mildew resistance Pl2,fruit firmness,skin colour,flavour intensity,and acidity.The availability of this set of validated trait-associated SNP markers,which can be used individually on multiple genotyping platforms available to various apple breeding programmes or re-designed using the flanking sequences,represents a large translational genetics step from genomics to crop improvement of apple. | David Chagné Stijn Vanderzande Chris Kirk Natalie Profitt Rosemary Weskett Susan E.Gardiner Cameron P.Peace Richard K.Volz Nahla V.Bassil | 2019 | Horticulture Research2019，6，1： | 3 |
| 2 | Fractures of the os calcis in children 显示文摘 | Wiley JJ Profitt A | 1984 | Clin Orthop relat res1984，，： | 1 |
| 3 | Working with DICOM craniofacial images显示文摘 | Grauer D LSH Cevidanes Profitt WR | 2009 | Am J Orthod Dentofacial Orthop2009，136，3： | 1 |
| 4 | Settling velocity characteristics of sediment detached from a soil surface by raindrop impact显示文摘 | Profitt A P B Rose C W Lovell C J | 1993 | Catena1993，20，： | 1 |
| 5 | Expression of thyroid transcription factor-1,surfactant proteins,type Ⅰ cell-associated antigen,and Clara cell secretory protein in pulmonary hypoplasia显示文摘 | Zhou H Morotti RA Profitt SA | 2001 | Pediatr Dev Pathol2001，4，4： | 1 |
| 6 | Au nanoparticle conjugation for impedance and capacitance signal amplifi- cation in biosensors显示文摘 | Wang J B Profitt J A Pugia M J | 2006 | Anal Chem2006，78，6： | 1 |
| 7 | High-Sensitivity Dye Binding Assay for Albumin in Urine 显示文摘 | Pugia M J Lott J A Profitt J A | 1999 | J Clin Lab Anal1999，13，4： | 1 |
| 8 | Mechanical principles in orthodontic force control 显示文摘 | Profitt WR Fields HW JR | 2000 | Contemporary Orthodontics2000，31，5： | 1 |
| 9 | Reduction of diphenylethylenes and related compounds with magnesium in methanol 显示文摘 | Profitt J A Ong H H | 1979 | J Org Chem1979，44，22： | 1 |
| 10 | Electrophysiological effects of myocardial stretch and mechanical determinants of stretch-activated arrhythmias显示文摘 | Franz MR Cima R Wang D Profitt D Kurz R | 1992 | Circulation1992，86，3： | 1 |
| 11 | Fractures of the oscalcis in children 显示文摘 | Wiley JJ Profitt A | 1984 | Clin Orthop Relat Res1984，，188： | 1 |
| 12 | Expression of thyroid transcription factor-1,surfactant proteins,type Ⅰ cell-associated antigen,and Clara cell secretory protein in pulmonary hypoplasia显示文摘 | Zhou H Morotti RA Profitt SA Langston C Wert SE Whitsett JA | 2001 | Pediatr Dev Pathol2001，4，4： | 1 |
| 13 | Expression of thyroid transcription factor-1 in congenital cystic adenomatoid malformation of the lung显示文摘 | Morotti RA Gutierrez MC Askin F Profitt SA Wert SE Whitsett JA | 2000 | Pediatr Dev Pathol2000，3，5： | 1 |
| 14 | Sensitive radiochemical assay forInosine 5'-monophosphate dehydrogenase and determination of activity in murine Tumor and tissue extracts显示文摘 | Profitt RT Pathak VK Villacorte DG | | 0，，： | 1 |
| 15 | Contrasting effects of wolves and human hunters on elk behavioral responses to predation risk显示文摘 | Profitt K M Grigg J L Hamlin K L | | 0，，03： | 1 |
| 16 | Fractures of the os calcis in children 显示文摘 | Wiley J J Profitt A | 1984 | Clin Orthop1984，，188： | 1 |
| 17 | Equilibrium theory revisited: factors influencing position of teeth显示文摘 | Profitt WR | 1978 | Angle Orthod1978，48，3： | 1 |
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