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502篇 您的检索式：作者名="Prather"

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	题名	作者	年代	出处	被引量
1	Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer显示文摘Transgenic somatic cell nuclear transfer is a very promising route for producing transgenic farm ani-mals. Research on GFP transgenic pigs can provide useful information for breeding transgenic pigs, human disease models and human organ xenotransplantation. In this study, a liposomal transfection system was screened and transgenic embryos were reconstructed by nuclear transfer of GFP positive cells into enucleated in vitro matured oocytes. The development of reconstructed embryos both in vitro and in vivo was observed, and GFP expression was determined. The results showed that porcine fe-tal-derived fibroblast cells cultured with 4.0 μL/mL liposome and 1.6 μg/mL plasmid DNA for 6 h re-sulted in the highest transfection rate (3.6%). The percentage of GFP reconstructed embryos that de-veloped in vitro to the blastocyst stage was 10%. Of those the GFP positive percentage was 48%. Re-constructed transgenic embryos were transferred to 10 recipients. 5 of them were pregnant, and 3 de-livered 6 cloned piglets in which 4 piglets were transgenic for the GFP as verified by both GFP protein expression and GFP DNA sequence analysis. The percentage of reconstructed embryos that resulted in cloned piglets was 1.0%; while the percentage of piglets that were transgenic was 0.7%. This is the first group of transgenic cloned pigs born in China, marking a great progress in Chinese transgenic cloned pig research.	LIU ZhongHua SONG Jun WANG ZhenKun TIAN JiangTian KONG QingRan ZHENG Zhong YIN Zhi GAO Li MA HaiKun SUN Shuang LI YuTian WANG HongBin R S PRATHER	2008	Chinese Science Bulletin2008，53，7：	8
2	Antenna design for a massive multiple input environmental sensor network显示文摘	J. Craig Prather Michael Bolt Haley Harrell John Manobianco Mark L. Adams	2016	Digital Communications and Networks2016，2，4：	2
3	Nuclear transplantation in the bovine embryo:assessment of donor nuclei recipient oocyte显示文摘	Prather RS Barnes FL Sims ML	1987	Biol Reprod1987，37，：	1
4	Stratospheric ozone depletion and future trends of atmospheric chlorine and bromine显示文摘	PRATHER M J WATSON R T	1990	Nature1990，344，：	1
5	Keeping innovation alive after consult-ants leave显示文摘	PRATHER C W	2000	Research Technology Management2000，43，5：	1
6	Tropospheric chemistry: a global perspective 显示文摘	J A Logan M J Prather S C Wofsy	1981	J Geophys Res1981，86，8：	1
7	A toxicity index of skin and wound cleansers used on in vitro fibroblasts and keratinocytes 显示文摘	Wilson JR Mills JG Prather ID	2005	Adv Skin Wound Care2005，18，7：	1
8	Nucleartransplantation in the bovine embryo: as-sessmentof donor nuclei and recipient oocyte显示文摘	Prather R S	1987	Biol Reprod1987，37，：	1
9	Genetically engineeredpig models for human diseases 显示文摘	Prather RS Lorson M Ross J W	2013	Annu Rev Anita Biosci2013，1，：	1
10	CAB reviews : perspectives in agriculture, veterinary science 显示文摘	Ross J W Prather R S Whyte J J	2009	Nutr Nat Re- sour2009，4，38：	1
11	Atmospheric distributionof 85Kr simulated with a general circulation model 显示文摘	Jacob D J Prather M J Wofsy S C	1987	Journal of Geophysical Research1987，92，：	1
12	Strategies for activating nu- clear transfer oocytes显示文摘	Machaty Z Prather R S	1998	Rep Rod Fertil Dev1998，10，78：	1
13	Analysis of atmospheric aerosols显示文摘	Prather K A Hatch C D Grassian V H		0，，：	1
14	Developments in early-time (El) high-altitude electromagnetic pulse (HEMP) test methods显示文摘	Wraight A Prather W D Sabath F	2013	IEEE Transactions on Electromagnetic Compatibility2013，55，3：	1
15	Cloning embryos by nuclear transfer显示文摘	Prather R S Barnes F L	1990	Reprod Fertil1990，41，：	1
16	Prospective measurement of function and pain in patients with non-neoplastic compression fractures treated with vertebroplasty显示文摘	Prather H Vandillen L Metzler JP	2006	J Bone Joint Surg(AM)2006，88，2：	1
17	Negative affec- tive responses to a speech task predict changes in interleukin (IL)-6显示文摘	Carroll JE Low CA Prather AA	2011	Brain behavior and immunity2011，25，：	1
18	Practical considerations for the in vitro production of pig embryos显示文摘	R.S. Prather B.N. Day	1998	Theriogenology1998，，1：	1
19	Pregnancy - related eonstipation显示文摘	Prather CM	2004	Curt Gastroen- terol ReP2004，6，：	1
20	Pregnancy-related constipation 显示文摘	Prather CM	2004	Curr Gastroenterol Rep2004，6，5：	1

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