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| 1 | Celastrus orbiculatus extract induces mitochondrial-mediated apoptosis in human hepatocellular carcinoma cells显示文摘OBJECTIVE:To investigate the apoptotic effects and underlying molecular mechanisms of Celastrus orbiculatus(C.orbiculatus) extract in human hepatocellular carcinoma cells.METHODS:Human hepatocellular carcinoma cells(HCCLM6) were treated with C.orbiculatus extract(COE) at different nontoxic concentrations(10,20,40,80,and 160 μg/mL).The effect of COE on HC-CLM6 viability was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assays.Cellular apoptosis following COE treatment was assessed by flow cytometry and western blot analysis.RESULTS:COE significantly inhibited cell viability and induced apoptosis of HCCLM6 cells in a dose-dependent manner.Apoptosis was accompanied by increased Bax expression and decreased Bcl-2 expression.In addition,COE treatment led to the release of cytochrome c,activation of caspase-3,and cleavage of poly(ADP-ribose) polymerase(PARP).Furthermore,activation of extracellular signal-regulated kinase(ERK),p38 kinase,and c-Jun N-terminal kinase(JNK) phosphorylation,and down-regulation of Akt phosphorylation was observed.CONCLUSION:COE induces mitochondrial-mediated,caspase-dependent apoptosis in HCCLM6 cells,which might be attributed to the activation of mitogen-activated protein kinase(MAPK) and inhibition of Akt signaling pathways.These data suggest that COE may be a potential treatment for human hepatocellular carcinoma. | Hua Zhang Yayun Qian Yanqing Liu Guoqing Li Pingfang Cui Yaodong Zhu Hui Ma Xue Ji Shiyu Guo Hisamits Tadashi | 2012 | Journal of Traditional Chinese Medicine2012，32，4： | 18 |
| 2 | Effects of motilin and ursodeoxycholic acid on gastrointestinal myoelectric activity of different origins in fasted rats显示文摘AIM: To investigate gastrointestinal migrating myoelectric complex (MMC) and the effects of porcine motilin and ursodeoxycholic acid (UDCA) on MMC of gastrointestinal tract of different origins in fasted rats.METHODS: Three bipolar silver electrodes were chronically implanted on the antrum, duodenum and jejunum. Seven days later 24 experimental rats were divided into 2 groups.One group was injected with porcine motilin via sublingual vein at a dose of 20 lig/kg, the other group was perfused into stomach with UDCA. The gastrointestinal myoelectric activity was recorded 1 h before and 2 h after the test substance infusions into the rats.RESULTS: In all fasted rats a typical pattern of MMC was observed. Among the totally 68 activity fronts recorded in fasted rats under control, 67% started in duodenum, and 33% in antrum. MMC cycle duration and duration of phase III of antral origin were longer than those of duodenal origin. Administration of 20 lig/kg porcine motilin induced a premature antral phase III of antral origin. But perfusion into stomach with UDCA resulted in shorter MMC cycle duration, longer duration of phase III of duodenal origin,which were followed with shorter cycle duration and duration of antral phase III.CONCLUSION: In fasted rats, MMC could originate from antrum and duodenum respectively. The characteristics of MMC of different origins may contribute to the large variations within subjects. The mechanisms of different origins of phase III may be different. Porcine motilin and UDCA could affect MMC of different origins of the gastrointestinal tract in fasted state, respectively. | PingFang LeiDong Jin-YanLuo Xiao-LongWan Ke-XinDu Ning-LiChai | 2004 | World Journal of Gastroenterology2004，10，17： | 12 |
| 3 | Whole genome re-sequencing reveals evolutionary patterns of sacred lotus (Nelumbo nucifera)显示文摘Sacred lotus(Nelumbo nucifera or lotus) is an important aquatic plant in horticulture and ecosystems. As a foundation for exploring genomic variation and evolution among different germplasms, we re-sequenced 19 individuals from three cultivated temperate lotus subgroups(rhizome,seed and flower lotus), one wild temperate lotus subgroup(wild lotus), one tropical lotus group(Thai lotus) and an outgroup(Nelumbo lutea). Through genetic diversity and polymorphism analysis by non-missing SNP sites widely distributed in the whole genome, we confirmed that wild and Thai lotus exhibited greater differentiation with a higher genomic diversity compared to cultivated lotus. Rhizome lotus had the lowest genomic diversity and a closer relationship to wild lotus, whereas the genomes of seed and flower lotus were admixed. Genes in energy metabolism process and plant immunity evolved rapidly in lotus, reflecting local adaptation.We established that candidate genes in genomic regions with significant differentiation associated with temperate and tropical lotus divergence always exhibited highly divergent expression pattern. Together, this study comprehensive and credible interpretates important patterns of genetic diversity and relationships, gene evolution, and genomic signature from ecotypic differentiation of sacred lotus. | Longyu Huang Mei Yang Ling Li Hui Li Dong Yang Tao Shi Pingfang Yang | 2018 | Journal of Integrative Plant Biology2018，60，1： | 8 |
| 4 | Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99–125 lipase via adsorption显示文摘Magnetically modified palygorskite composites were synthesized with γ-Fe2O3 dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffraction, transmission electron microscopy, and vibrating sample magnetometer. Candida sp. 99–125 lipase was immobilized on magnetic palygorskite composites by physical adsorption with enzyme loading of41.5 mg·g-1support and enzyme activity of 2631.6 U·(g support)-1. The immobilized lipase exhibit better thermal and broader p H stability and excellent reusability compared with free lipase. | Ya Li Jicheng Hu Pingfang Han | 2015 | Chinese Journal of Chemical Engineering2015，23，5： | 6 |
| 5 | Attenuated phenotypes and analysis of a herpes simplex virus 1strain with partial deletion of the UL7, UL41 and LAT genes显示文摘We previously constructed a herpes simplex virus 1(HSV-1) UL7 mutant virus(M1) and showed that a partial deletion mutation of the UL7 gene led to a lower proliferative rate and an attenuated phenotype. Using the M1 mutant, we further modified the UL41 gene, which encodes another tegument protein, and the latency-associated transcript(LAT) gene. Observations of the resulting mutants with modified UL7 and UL41(M2) or UL7, UL41 and LAT(M3) genes indicated attenuated phenotypes, with lower proliferative ratios in various cells, non-lethal infections in mice and lower viral loads in nervous tissues compared with the wild-type strain. Furthermore, no LAT stable intron could be detected in the trigeminal ganglion of M3-infected animals. The results obtained with the three HSV-1 mutants indicate that the M3 mutant is an attenuated strain with low pathogenicity during both acute and latent infections. Together, the results support the use of the M3 mutant as a candidate for the development of an HSV-1 vaccine. | Xingli Xu Yingqiu Guo Shengtao Fan Pingfang Cui Min Feng Lichun Wang Ying Zhang Yun Liao Xiaolong Zhang Qihan Li | 2017 | Virologica Sinica2017，32，5： | 6 |
| 6 | A comparative study of the characteristics of two Coxsackie A virus type 16 strains (genotype B)显示文摘Coxsackie A virus is one of the major pathogens associated with hand, foot and mouth disease (HFMD). The etiological characteristics of Coxsackie A virus type 16 (CA16) are thought to correlate with the pathological process of its infection. Two CA16 strains that were isolated from a severe HFMD patient presented with different plaque forms. This observation, along with biological analysis, indicated that the differences in the strains' biological characteristics, such as proliferation kinetics and immunogenicity, correlate with differences in their pathogenicity toward neonatal mice. Furthermore, these differences are thought to be associated with the sequence of the 5′ non-coding region of the viral genome and the VP1 structural region sequence. The results suggest that the biological and genetic characteristics of the CA16 viral strains are relevant to their pathogenicity. | YANG ErXia ZHAO Heng ZHANG Ying LIU JianSheng LIAO Yun WANG LiChun CUI PingFang YANG LiXian LIU LongDing DONG ChengHong DONG ShaoZhong SHAO CongWen JIANG Li SUN Le LI QiHan | 2012 | Science China(Life Sciences)2012，55，4： | 5 |
| 7 | Fundamental CRISPR-Cas9 tools and current applications in microbial systems显示文摘Derived from the bacterial adaptive immune system,CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering.In this review,we outline the fundamental CRISPR tools that have been employed for strain optimization.These tools include CRISPR editing,CRISPR interference,CRISPR activation and protein imaging.To further characterize the CRISPR technology,we present current applications of these tools in microbial systems,including model-and non-model industrial microorganisms.Specially,we point out the major challenges of the CRISPR tools when utilized for multiplex genome editing and sophisticated expression regulation.To address these challenges,we came up with strategies that place emphasis on the amelioration of DNA repair efficiency through CRISPR-Cas9-assisted recombineering.Lastly,multiple promising research directions were proposed,mainly focusing on CRISPR-based construction of microbial ecosystems toward high production of desired chemicals. | Pingfang Tian Jia Wang Xiaolin Shen Justin Forrest Rey Qipeng Yuan Yajun Yan | 2017 | Synthetic and Systems Biotechnology2017，2，3： | 4 |
| 8 | Gene Expression Profile Changes in Germinating Rice显示文摘Water absorption is a prerequisite for seed germination.During imbibition,water influx causes the resumption of many physiological and metabolic processes in growing seed.In order to obtain more complete knowledge about the mechanism of seed germination,two-dimensional gel electrophoresis was applied to investigate the protein profile changes of rice seed during the first 48 h of imbibition.Thirtynine differentially expressed proteins were identified,including 19 down-regulated and 20 up-regulated proteins.Storage proteins and some seed development-and desiccation-associated proteins were down regulated.The changed patterns of these proteins indicated extensive mobilization of seed reserves.By contrast,catabolism-associated proteins were up regulated upon imbibition.Semi-quantitative real time polymerase chain reaction analysis showed that most of the genes encoding the down-or upregulated proteins were also down or up regulated at mRNA level.The expression of these genes was largely consistent at mRNA and protein levels.In providing additional information concerning gene regulation in early plant life,this study will facilitate understanding of the molecular mechanisms of seed germination. | Dongli He Chao Han Pingfang Yang | 2011 | Journal of Integrative Plant Biology2011，53，10： | 3 |
| 9 | Comparative Analysis of the Endosperm Proteins Separated by 2-D Electrophoresis for Two Cultivars of Hybrid Rice (Oryza sativa L,)显示文摘Liangyoupeijiu 是 two-parental-line,并且 Shanyou63 是 three-parental-line 混血儿米饭(Oryza sativa L.) 。尽管两个属于 indica 亚种,他们关于形态学,生理学和谷物质量有明显的差别。在内乳蛋白质作文的变化被比较电气泳动(2-DE ) 为混合米饭的这二栽培变种印射的 2-D 学习。在飞行团 spectrometry (MALDI-TOF/MS ) 的帮助矩阵的激光解吸附作用 / 电离时间以后分析, 19-kDa 血球素的一位 21-kDa 先锋为两栽培变种作为主要存储蛋白质被识别。peroxiredoxin 和种子成熟蛋白质的某 isoforms 被发现仅仅在 Shanyou63 存在,而 aldose reductase 和淀粉小粒界限淀粉 synthase 仅仅在 Liangyoupeijiu 被检测。这些数据可能为混合米饭的这二栽培变种的进一步比较的研究提供一个基础。 | Pingfang Yang Shihua Shen Tingyun Kuang | 2006 | Journal of Integrative Plant Biology2006，48，9： | 3 |
| 10 | A Recommendation Mechanism for Web Publishing Based on Sentiment Analysis of Microblog显示文摘Microblog is a social platform with huge user community and mass data. We propose a semantic recommendation mechanism based on sentiment analysis for microblog. Firstly, the keywords and sensibility words in this mechanism are extracted by natural language processing including segmentation, lexical analysis and strategy selection. Then, we query the background knowledge base based on linked open data(LOD) with the basic information of users. The experiment result shows that the accuracy of recommendation is within the range of 70%-89% with sentiment analysis and semantic query. Compared with traditional recommendation method, this method can satisfy users' requirement greatly. | TIAN Pingfang ZHU Zhonghua XIONG Li XU Fangfang | 2015 | Wuhan University Journal of Natural Sciences2015，20，2： | 2 |
| 11 | A Review of Co-processing of Kitchen Waste and Municipal Residual Sludge显示文摘In this paper,the methods of kitchen waste disposal in recent years were summed up,and the advantages and disadvantages of the current treatment methods were analyzed. At the same time,it has been found that the co-processing of kitchen waste and residual sludge not only has dealt with both kitchen waste and residual sludge,but also the carbon and nitrogen ratio and moisture content of the mixture are balanced,and increases the processing efficiency significantly. It has a certain processing advantage. At present,most research is about the anaerobic digestion of kitchen waste and residual sludge,and the process is more mature,but the study of VFAs accumulation is still insufficient. Aerobic composting is still in the early stage of development,and there are few studies on it at home and abroad. Meanwhile,prospects for the kitchen waste recycling were made in this article. | Han Houfeng Zhuang Pingfang Liu Mei Rong Zhenying Weng Yisong Chen Qingguo Sun Jingya | 2018 | Meteorological and Environmental Research2018，9，4： | 2 |
| 12 | Ultrasonic treatment on activated sewage sludge from petro-plant for reduction显示文摘 | Xuan Yin Xiaoping Lu Pingfang Han | 2006 | Ultrasonics2006，，44： | 1 |
| 13 | Ef-fects of Ultrasound on Oily Sludge Deoiling 显示文摘 | Xu Ning Wang Wenxian Han Pingfang | 2009 | JHazard Mater2009，171，123： | 1 |
| 14 | Desalting and dewatering of crude oil in ultrasonic standing wave field显示文摘 | Ye Guoxiang Lu Xiaoping Han Pingfang | 2010 | Journal of Petroleum Science and Engineering2010，70，： | 1 |
| 15 | Effects of ultrasound on oily sludge deoiling显示文摘 | XU Ning WANG Wenxiang HAN Pingfang | | 0，，： | 1 |
| 16 | Desalting and dewatering of crude oil in ultrasonic standing wave field显示文摘 | Ye Guoxiang Lu Xiaoping Han Pingfang | 2010 | Journal of Petroleum Science and Engineering2010，70，： | 1 |
| 17 | Identifying RNA Modifications by Direct RNA Sequencing Reveals Complexity of Epitranscriptomic Dynamics in Rice显示文摘Transcriptome analysis based on high-throughput sequencing of a cDNA library has been widely applied to functional genomic studies.However,the cDNA dependence of most RNA sequencing techniques constrains their ability to detect base modifications on RNA,which is an important element for the post-transcriptional regulation of gene expression.To comprehensively profile the N^(6)-methyladenosine(m^(6)A)and N^(5)-methylcytosine(m5 C)modifications on RNA,direct RNA sequencing(DRS)using the latest Oxford Nanopore Technology was applied to analyze the transcriptome of six tissues in rice.Approximately 94 million reads were generated,with an average length ranging from 619 nt to 1013 nt,and a total of 45,707 transcripts across 34,763 genes were detected.Expression profiles of transcripts at the isoform level were quantified among tissues.Transcriptome-wide mapping of m^(6)A and m5 C demonstrated that both modifications exhibited tissue-specific characteristics.The transcripts with m^(6)A modifications tended to be modified by m5 C,and the transcripts with modifications presented higher expression levels along with shorter poly(A)tails than transcripts without modifications,suggesting the complexity of gene expression regulation.Gene Ontology analysis demonstrated that m^(6)A-and m5 C-modified transcripts were involved in central metabolic pathways related to the life cycle,with modifications on the target genes selected in a tissue-specific manner.Furthermore,most modified sites were located within quantitative trait loci that control important agronomic traits,highlighting the value of cloning functional loci.The results provide new insights into the expression regulation complexity and data resource of the transcriptome and epitranscriptome,improving our understanding of the rice genome. | Feng Yu Huanhuan Qi Li Gao Sen Luo Rebecca Njeri Damaris Yinggen Ke Wenhua Wu Pingfang Yang | 2023 | Genomics, Proteomics & Bioinformatics2023，21，4： | 1 |
| 18 | Information measure for performace of image fusion 显示文摘 | Qu Guihong Zhang Dali Yan Pingfang | 2002 | Electronics Letters2002，38，7： | 1 |
| 19 | A method and case study of thermal response test with unstable heat rate显示文摘 | Hu Pingfang Meng Qingfeng Sun Qiming | 2012 | Energy and Buildings2012，48，5： | 1 |
| 20 | Basic and clinical aspects of non-neuronal acetylcholine:expression of non-neuronal acetylcholine in lung cancer provides a new target for cancer therapy显示文摘 | PINGFANG S ELIOT RS | 2008 | J Pharmacol Sci2008，106，2： | 1 |
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