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您的检索式：作者名="Yan Pingfang"
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| 1 | Fundamental CRISPR-Cas9 tools and current applications in microbial systems显示文摘Derived from the bacterial adaptive immune system,CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering.In this review,we outline the fundamental CRISPR tools that have been employed for strain optimization.These tools include CRISPR editing,CRISPR interference,CRISPR activation and protein imaging.To further characterize the CRISPR technology,we present current applications of these tools in microbial systems,including model-and non-model industrial microorganisms.Specially,we point out the major challenges of the CRISPR tools when utilized for multiplex genome editing and sophisticated expression regulation.To address these challenges,we came up with strategies that place emphasis on the amelioration of DNA repair efficiency through CRISPR-Cas9-assisted recombineering.Lastly,multiple promising research directions were proposed,mainly focusing on CRISPR-based construction of microbial ecosystems toward high production of desired chemicals. | Pingfang Tian Jia Wang Xiaolin Shen Justin Forrest Rey Qipeng Yuan Yajun Yan | 2017 | Synthetic and Systems Biotechnology2017，2，3： | 4 |
| 2 | Information measure for performace of image fusion 显示文摘 | Qu Guihong Zhang Dali Yan Pingfang | 2002 | Electronics Letters2002，38，7： | 1 |
| 3 | Information measure for performance of image fusion 显示文摘 | Qu Guihong Zhang Dali Yan Pingfang | 2002 | Electronics Letters2002，38，7： | 1 |
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