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| 1 | Proteomic Comparison of Two-Dimensional Gel Electrophoresis Profiles from Human Lung Squamous Carcinoma and Normal Bronchial Epithelial Tissues显示文摘Differential proteome profiles of human lung squamous carcinoma tissue compared to paired tumor-adjacent normal bronchial epithelial tissue were established and analyzed by means of immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). The results showed that well-resolved, reproducible 2-DE patterns of human lung squamous carcinoma and adjacent normal bronchial epithelial tissues were obtained under the condition of 0.75-ug protein-load. The average deviation of spot position was 0.733+0.101 mm in IEF direction, and 0.925+0.207 mm in SDS-PAGE direction. For tumor tissue, a total of 1241±88 spots were detected, 987±65 spots were matched with an average matching rate of 79.5%. For control, a total of 1190+72 spots were detected, and 875±48 spots were matched with an average matching rate of 73.5%. A total of 864±34 spots were matched between tumors and controls.Forty-three differential proteins were characterized: some proteins were related to oncogenes, and others involved in the regulation of cell cycle and signal transduction. It is suggested that the differential proteomic approach is valuable for mass identification of differentially expressed proteins involved in lung carcinogenesis.These data will be used to establish human lung cancer proteome database to further study human lung squamous carcinoma. | CuiLi XianquanZhan MaoyuLi XiaoyingWu FengLi JianlingLi ZhiqiangXiao ZhuchuChen XuepingFeng PingChen JingyunXie SongpingLiang | 2003 | Genomics, Proteomics & Bioinformatics2003，1，1： | 16 |
| 2 | Relationship between proliferative activity of cancer cells and clinicopathological factors in patients with esophageal squamous cell carcinoma显示文摘AIM: To assess whether the molecular markers of malignant tumors could improve the understanding of tumor characteristics, and to observe the characteristics of expression of cell cycle markers Ki-67 and cydin A in esophageal carcinoma and to analyze the relationship between proliferative activity of cancer cells and clinicopathological factors.METHODS: Seventy of surgically resected esophageal squamous cell carcinoma (SCC) were examined by immunohistochemistry utilizing commercially available antibodies. Nuclear staining was regarded as a positive result. At least 50 fields in each tumor and non-tumor section were evaluated at a medium power (x200) to determine the proportion of tumor cells and the staining intensity of nuclei in the entire sections.RESULTS: Ki-67 and cyclin A were only expressed in base cells of normal esophageal mucosa. The positive immunostaining of nuclei of SCC was significantly higher than that in normal esophageal mucosa (t= 13.32 and t= 7.52,respectively, P<0.01). The distribution of positively stained was more diffuse and stronger in poorly differentiated SCC. Both Ki-67 and cyclin A expressions were related to histological grades of tumors (t = 3.5675 and t = 3.916; t= 2.13, respectively, P<0.05) but not to the sex and age of the patients, tumor size, lymphatic invasion, location, or stage grouping.CONCLUSION: The proliferative activity of cancer cells may be understood by immunohistochemistry of Ki-67 and cyclin A in Chinese patients with esophageal SCC. These cell cycle markers may serve as an indicator of cancer cell proliferation rate. The overexpression of cell cycle markers Ki-67 and cyclin A suggests the poor SCC differentiation in patients with esophageal carcinoma. | Jun-XingHuang WeiYan Zheng-XiangSong Rong-YuQian PingChen EevaSalminen JormaToppari | 2005 | World Journal of Gastroenterology2005，11，19： | 8 |
| 3 | Recombinant fibronectin polypeptide antagonizes hepatic failure induced by endotoxin in mice显示文摘AIM: To study the preventive effect of recombinant human fibronectin (rhFN) polypeptide on hepatic failure in- duced by endotoxin in mice. METHODS: A cDNA fragment coding for Ile1363-Tyr1725 of human FN was inserted into the PinPoint Xa-3 plasmid, and the constructed plasmid was transformed into E coli BL21 (DE3) cells, and then the expression of rhFN polypeptide in DE3 cells was identified through SDS-PAGE. The target protein from the supernatant of bacteria lysate was purified through biotin-affinity chromatography. The bioactivity of the purified rhFN polypeptide was determined with cell adhesiveactivity. The survival rate was observed in endotoxemia mice injected with rhFN polypeptide. The tissue damage in hepatocyte was detected using histology, ultrastructure, and DNA fragmentation assay. RESULTS: The expression of rhFN polypeptide reached approximately 20 % of the total cellular protein. The adhesion ability of rhFN polypeptide was concentration-dependent. The value of EC50 was 0.8 nmol/L. The survival rate of endotoxemia mice sensitized by D-galactosamine (D-GalN) was 60 % in rhFN polypeptide treated group, while that of endotoxemia mice sensitized by D-GalN was 20 % in the control group (P<0.01). Histopathology showed that less necrosis occurred on the hepatocyte of endotoxemia mice in- jected with rhFN polypeptide compared with saline control. Ultrastructure and DNA fragmentation assay showed that no apoptotic hepatocyte was observed in the liver of rhFN-treated endotoxemia mice. CONCLUSION: Re- combinant fibronectin polypeptide antagonizes hepatic failure induced by endotoxin in mice. | YongWU Yuan-zhongCHEN Hui-fangHUANG PingCHEN | 2004 | Acta Pharmacologica Sinica2004，25，6： | 7 |
| 4 | Structural basis for site-specific reading of unmodified R2 of histone H3 tail by UHRF1 PHD finger显示文摘 | ChengkunWang JieShen ZhongzhengYang PingChen BinZhao WeiHu WenxianLan XiaotianTong HoumingWu GuohongLi ChunyangCao | 2011 | Cell Research2011，21，9： | 5 |
| 5 | Heterogeneity in predisposition of hepatic cells to be induced into pancreatic endocrine cells by PDX-1显示文摘AIM: The role of Pancreatic and Duodenal Homeobox-1(PDX-1) as a major regulator of pancreatic development determines the function and phenotype of β cell. In this study, potential plasticity of liver cells into pancreatic endocrine cells induced by PDX-1 was evaluated.METHODS: Human hepatoma cell line HepG2 was stably transfected with mammalian expression plasmid pcDNA3-PDX encoding human PDX-1 gene. Ectopic expression of PDX-1 and insulin were detected by RT-PCR,Western blot and/or immunostaining. PDX-1+ HepG2 cells were transplanted under renal capsule of STZ-induced diabetic nude mice (n = 16) to examine the inducing effect in vivo.RESULTS: Exogenous PDX-1 transgene was proved to express effectively in HepG2 cell at both mRNA and protein levels. The expression of endogenous insulin and some βcell-specific differentiation markers and transcription factors were not induced in PDX-1+ HepG2 cells. When transplanted under renal capsule of STZ-induced diabetic nude mice, PDX-1+ HepG2 cells did not generate insulinproducing cells. These data indicated that stable transfected PDX-1 could not convert hepatoma cell line HepG2 to pancreatic cells in vitro or in vivo. Mature hepatocytes might need much more complicated or rigorous conditions to be shifted to insulin-producing cells.CONCLUSION: The expression of exogenous PDX-1 is not sufficient to induce relatively mature hepatocytes differentiating into insulin-producing cells. | ShunLu Wei-PingWang Xiao-FeiWang Zong-MeiZheng PingChen Kang-TaoMa Chun-YanZhou | 2005 | World Journal of Gastroenterology2005，11，15： | 4 |
| 6 | Objective assessment of bradykinesia in Parkinson’s disease using evolutionary algorithms:clinical validation显示文摘Background:There is an urgent need for developing objective,effective and convenient measurements to help clinicians accurately identify bradykinesia.The purpose of this study is to evaluate the accuracy of an objective approach assessing bradykinesia in finger tapping(FT)that uses evolutionary algorithms(EAs)and explore whether it can be used to identify early stage Parkinson’s disease(PD).Methods:One hundred and seven PD,41 essential tremor(ET)patients and 49 normal controls(NC)were recruited.Participants performed a standard FT task with two electromagnetic tracking sensors attached to the thumb and index finger.Readings from the sensors were transmitted to a tablet computer and subsequently analyzed by using EAs.The output from the device(referred to as'PD-Monitor')scaled from−1 to+1(where higher scores indicate greater severity of bradykinesia).Meanwhile,the bradykinesia was rated clinically using the Movement Disorder Society-Sponsored Revision of the Unified Parkinson’s Disease Rating Scale(MDS-UPDRS)FT item.Results:With an increasing MDS-UPDRS FT score,the PD-Monitor score from the same hand side increased correspondingly.PD-Monitor score correlated well with MDS-UPDRS FT score(right side:r=0.819,P=0.000;left side:r=0.783,P=0.000).Moreover,PD-Monitor scores in 97 PD patients with MDS-UPDRS FT bradykinesia and each PD subgroup(FT bradykinesia scored from 1 to 3)were all higher than that in NC.Receiver operating characteristic(ROC)curves revealed that PD-Monitor FT scores could detect different severity of bradykinesia with high accuracy(≥89.7%)in the right dominant hand.Furthermore,PD-Monitor scores could discriminate early stage PD from NC,with area under the ROC curve greater than or equal to 0.899.Additionally,ET without bradykinesia could be differentiated from PD by PD-Monitor scores.A positive correlation of PD-Monitor scores with modified Hoehn and Yahr stage was found in the left hand sides.Conclusions:Our study demonstrated that a simple to use device employing classifiers derived from EAs could not only be used to accurately measure different severity of bradykinesia in PD,but also had the potential to differentiate early stage PD from normality. | Chao Gao Stephen Smith Michael Lones Stuart Jamieson Jane Alty Jeremy Cosgrove Pingchen Zhang Jin Liu Yimeng Chen Juanjuan Du Shishuang Cui Haiyan Zhou Shengdi Chen | 2018 | Translational Neurodegeneration2018，7，1： | 3 |
| 7 | Mechanism for the Nanometer Scale Modification on HOPG Surface by Scanning Tunneling Microscope显示文摘A technique applying voltage across the tunnel junction for wnting permanent features on graphite with lines about 10nm wiidth using our home-built scanning tunneling microscope has been presented.Multiple-tip effects while modifying the surface are often met.The phenomciifi are explained in terms of the strong electric field existing at the junction between the tip an<7 the surface.In addition,the shape of the structure indented by the tip is in agreement with that of the tip. | WANG Zhonghuai DAI Changchun ZHANG Pingchen HUANG Guizhen LI Renli GUO Yi BAI Chunli | 1993 | Chinese Physics Letters1993，10，9： | 2 |
| 8 | Application of fault diagnosis in rotating machinery by using time series analysis with neural network显示文摘 | Wang C C Kang Yuan Shen Pingchen | 2010 | Expert Systems with Applications2010，37，2： | 1 |
| 9 | Chronic obstructive pulmonary disease in the absence of chronic bronchitis in China显示文摘 | MingLU WanzhenYAO NanshanZHONG YuminZHOU ChenWANG PingCHEN JianKANG ShaoguangHUANG BaoyuanCHEN ChangzhengWANG DiantaoNI XiaopingWANG DaliWANG ShengmingLIU JiachunLU NingSHEN PixinRAN | 2010 | Respirology2010，，7： | 1 |
| 10 | The Complete Genome Sequence of Two Isolates of Southern rice black‐streaked dwarf virus, a New Member of the Genus Fijivirus显示文摘 | QiangWang JianYang Guo‐HuiZhou Heng‐MuZhang Jian‐PingChen Michael J.Adams | 2010 | Journal of Phytopathology (鈥?)2010，，11： | 1 |
| 11 | Present-day deformation of the Qaidam basin with implications for intra-continental tectonics 显示文摘 | Wang Pingchen Chu Yungchen John L N | 1999 | Tectonophysics1999，305，： | 1 |
| 12 | Evaluation of the adjuvant properties of astragalus membranaceus and scutellaria baicalensis GEORGI in the immune protection induced by UV-attenuated toxoplasma gondii in mouse models显示文摘 | XIAO Yanyang SHI Guanghuang J]AN Pingchen | 2010 | Vaccine2010，28，3： | 1 |
| 13 | Appli cations of Fault Diagnosis in Rotating Machinery by Using Time Series Analysis with Neural Network显示文摘 | Wang C C Kang Yuan Shen Pingchen | | 0，，2： | 1 |
| 14 | The Complete Genome Sequence of Two Isolates of Southern rice black‐streaked dwarf virus, a New Member of the Genus Fijivirus显示文摘 | QiangWang JianYang Guo‐HuiZhou Heng‐MuZhang Jian‐PingChen Michael J.Adams | 2010 | 2010 (11‐)2010，，11： | 1 |
| 15 | Ammonia‐oxidizing archaea: important players in paddy rhizosphere soil?显示文摘 | Xue‐PingChen YueXia Ju‐PeiShen | 2008 | Environmental Microbiology2008，，8： | 1 |
| 16 | Appli- cations of Fault Diagnosis in Rotating Machinery by Using Time Series Analysis With Neural Network 显示文摘 | Wang C Kang Yuan Shen Pingchen | 2010 | Expert Systems with Applications2010，37，2： | 1 |
| 17 | Internal Resources, External Network, and Competitiveness during the Growth Stage: A Study of Taiwanese High‐Tech Ventures<sup>1</sup>显示文摘 | Lei‐YuWu Chun‐JuWang Cheng‐PingChen Lee‐YunPan | 2008 | Entrepreneurship Theory and Practice2008，，3： | 1 |
| 18 | Applications of fault diagnosis in rotating machinery by using time series analysis with neural network显示文摘 | Wang Chun-Chieh Kang Yuan Shen Pingchen | 2010 | Ex- pert System with Apphcation2010，37，2： | 1 |
| 19 | Ammonia‐oxidizing archaea: important players in paddy rhizosphere soil?显示文摘 | Xue‐PingChen YueXia Ju‐PeiShen | 2008 | Environmental Microbiology2008，，8： | 1 |
| 20 | Applications of fault diagnosis in rotating machinery by using time series analysis with neural network 显示文摘 | ChunChieh Wang Yuan Kang PingChen Shen | 2010 | Expert System with Application2010，372，： | 1 |
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