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| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | Divalent metal transporter, iron, and Parkinson's disease: A pathological relationship显示文摘 | Hyun-pil Lee Xiongwei Zhu Gang Liu Shu G Chen George Perry Mark A Smith Hyoung-gon Lee | 2010 | Cell Research2010，20，4： | 5 |
| 3 | Human acute myelogenous leukemia stem cells are rare and heterogeneous when assayed in NOD/SCID/IL2R[gamma]c-deficient mice显示文摘 | Sarry Jean-Emmanuel Murphy Kathleen Perry Robin Sanchez Patricia V Secreto Anthony Keefer Cathy Swider Cezary R Strzelecki Anne-Claire Cavelier Cindy Récher Christian Mansat-De Mas Véronique Delabesse Eric Danet-Desnoyers G Carroll Martin | 2011 | Journal of Clinical Investigation2011，，1： | 2 |
| 4 | Terawatt to petawatt subpicosecond lasers显示文摘 | Perry M D Mourou G | 1994 | Science1994，264，： | 2 |
| 5 | Effect of two opposing changes in photoperiod upon age at first egg and related traits in layer - hybrid pullets 显示文摘 | Lewis P D Perry G C Morris T R | 2003 | Journal of Agricultural Science Cambridge2003，140，： | 1 |
| 6 | Amyloid-beta-derived diffusible ligands cause impaired axonal transport of mitochondria in neurons显示文摘 | Wang X Perry G Smith MA | 2010 | Neurodegener Dis2010，7，13： | 1 |
| 7 | Effect of size and timing of photoperiod increase on age at first egg and subsequent performance of two breeds of laying hen显示文摘 | Lewis P Perry G Morris T | 1997 | Brit Pouh Sci1997，38，： | 1 |
| 8 | The effect of usage and size on tractor depreciation显示文摘 | Perry G M Bayaner A Nixon C J | 1990 | American Journal of Agricultural Economics1990，72，2： | 1 |
| 9 | Disorders of blood pressure regu- lation-role of catecholamine biosynthesis, release, and metabolism 显示文摘 | Currid G Freel EM Perry CG | 2012 | Curr Hypertens Rep2012，14，1： | 1 |
| 10 | Correlation of cholinergic abnormalities with senile plaques and mental test scores in senile dementia显示文摘 | Perry EK Tomlinson BE Blessed G Bergmann K Gibson PH Perry RH | | 0，，6150： | 1 |
| 11 | Nogo Domains and a Nogo Receptor:Implications for Axon Regeneration显示文摘 | Perry A Brittis John G Flanagan | 2001 | Neuron2001，，1： | 1 |
| 12 | Mini-and micro-channels:influence of axial conduction in the walls显示文摘 | Maranzana G Perry I Maillet D | 2004 | International Journal of Heat and Mass Transfer2004，47，1718： | 1 |
| 13 | RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer's disease 显示文摘 | Nunomura A Perry G Pappolla MA | 1999 | J Neurosci1999，19，： | 1 |
| 14 | Cytokine storm in a phase 1 trial 6f the anti-CD28 monoclonal antibody TGN1412 显示文摘 | Suntharalingam G Perry MR Ward S | 2006 | N Engl J Med2006，355，10： | 1 |
| 15 | A model for predicting the age at sexual maturity for growing pullets of layer strains given a single change in photoperiod 显示文摘 | Lewis P D Morris T R Perry G C | 2002 | Journal of Agricultural Science Cambridge2002，130，： | 1 |
| 16 | Glomerulosclerosis: aparaneoplastic phenomenon? 显示文摘 | LOI S PERRY G J STANDISH H | 2004 | Nephrology (Carlton)2004，9，6： | 1 |
| 17 | Retailer power and supplier welfare:the case of Wal-Mart显示文摘 | Bloom P N Perry V G | 2001 | Journal of Retailing2001，77，： | 1 |
| 18 | Effect of constant and of changing photoperiod on plasma LH and FSH concentrations and age at first egg in layer strains of domestic pullets显示文摘 | Lewis P D Perry G C Morris T R | 1998 | Br Poult Sci1998，39，： | 1 |
| 19 | Depreciation patterns for agricultural machinery显示文摘 | Cross T L Perry G M | 1995 | American Journal of Agricultural Economics1995，77，1： | 1 |
| 20 | Natural oxidant balance in Parkinson disease显示文摘 | Perry G Moreira PI Siedlak SL | | 0，，12： | 1 |
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