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| 1 | 枯草杆菌ylyA基因的绿色荧光蛋白标记显示文摘【目的】本研究对枯草杆菌ylyA基因进行荧光标记以便对其产物YlyA在菌体中的位置进行初步观察。【方法】以不同菌株基因组DNA为模板,对ylyA基因进行PCR扩增和序列分析;重新设计引物扩增全长的ylyA并将其克隆到载体pSG1729中,形成gfpmut1-ylyA融合而构建重组载体pNG426;将pNG426转化枯草杆菌168菌株,双交换使gfpmut1-ylyA插入染色体的amyE位点,用碘染色法和菌落PCR对阳性转化子BS363进行鉴定。NA固体培养基上生长的BS363经0.5%木糖诱导表达后,利用表面荧光显微镜技术进行观察。【结果】通过对多个PCR产物的序列分析确定了ylyA基因的正确序列以及正确的翻译起始位点;成功将重组载体pNG426转化枯草杆菌得到了BS363菌株;荧光检测结果表明GFP标记的YlyA分布于菌体的外周,在位置上靠近细胞膜并与之平行排列。【结论】生长缓慢的BS363菌体,在0.5%木糖诱导下产生的荧光标记YlyA蛋白分布在细胞外周,可能在膜生物学中发挥作用。 | 霍乃蕊 Lewis P J | 2008 | 微生物学报2008，48，6： | 7 |
| 2 | Comparison of transpiration rates among saltcedar, cottonwood and willow trees by sap flow and canopy temperature methods显示文摘 | PAMELA L Nagler EDWARD P Glenn THOMPSON T Lewis | 2003 | Agri and For Meter2003，116，： | 2 |
| 3 | Effect of two opposing changes in photoperiod upon age at first egg and related traits in layer - hybrid pullets 显示文摘 | Lewis P D Perry G C Morris T R | 2003 | Journal of Agricultural Science Cambridge2003，140，： | 1 |
| 4 | Critical design issues for airbreathing hypersonic waverider missiles 显示文摘 | Ryan P Starkey M Lewis J | 2001 | Journal of Spacecraft and Rockets2001，38，4： | 1 |
| 5 | A role for the AKT1 potassium channel in plant nutrition显示文摘 | Hirsch R E Lewis B D Spalding E P | 1998 | Science1998，280，： | 1 |
| 6 | Symposium:carbohudrate methodology,metabolism,and nutritional implications in dairy cattle:Methods for dietary fibre,neutral detergent fibre,and nonstarch polysaccharides in relation to animal nutrition显示文摘 | Van Scest P J Robertson J B Lewis B A | 1991 | Journal of Dairy Science1991，74，10： | 1 |
| 7 | Synthesis of new bis (acetylide)-substituted fluorene derivatives and their bimetallic and polymeric complexes显示文摘 | Lewis J Raithby P R Wong W Y | 1998 | J Organomet Chem1998，556，： | 1 |
| 8 | D2 or not D2? The gastrectomy question显示文摘 | Lewis WG Edwards P Jonathan DB | 2002 | Gastric Cancer2002，5，1： | 1 |
| 9 | The effect of excess protein on growth performance and protein metabolism of finishing barrows and gilts显示文摘 | Chen H Y Lewis A J Miller P S | 1999 | Journal of Animal Science1999，77，： | 1 |
| 10 | Human immunodeficiency virus infection of cells arrested in the cell cycle显示文摘 | Hensel M Emerman M | 1992 | EMBO J1992，11，8： | 1 |
| 11 | Super-resolution land cover pattern prediction using a Hopfield neural network 显示文摘 | TATEM A J LEWIS H G ATKINSON P M NIXON M S | 2002 | Remote Sensing of Environment2002，，： | 1 |
| 12 | Improved Surface Properties of Polymer Materisls by Multiple Lon Beam Treatment显示文摘 | Lee E H Lewis M B Blau P J | 1991 | Mater Res1991，6，3： | 1 |
| 13 | Geochemical characteristics of oils from Qaidam,Shanganning and Jianghan basins,China显示文摘 | PHILP R P FAN P LEWIS C A | 1991 | Journal of Southeast Asian Earth Sciences1991，5，3： | 1 |
| 14 | Effect of size and timing of photoperiod increase on age at first egg and subsequent performance of two breeds of laying hen显示文摘 | Lewis P Perry G Morris T | 1997 | Brit Pouh Sci1997，38，： | 1 |
| 15 | New Triterpenoid Extractives from Lemaireocereus chichipe显示文摘 | Khong P W Lewis K G | 1975 | Aust J Chem1975，28，： | 1 |
| 16 | Metal enrichment in bauxites by deposition of chemically mature aeoli- an dust 显示文摘 | Brimhall G H Lewis X J Ague J J Detroit W E Hanpel J Rix P | 1988 | Nature1988，333，： | 1 |
| 17 | Strain localization in geomaterials 显示文摘 | Desrues J B6suelle P Lewis H | 2007 | Geological Society London Special Publications2007，289，： | 1 |
| 18 | Active magnetic control of oscillatory axial shaft vibrations in ship shaft transmission systems part 1 : System natural frequencies and Laboratory scale model显示文摘 | LEWIS D W ALLAIRE P E | 1989 | Tribology transactions1989，32，2： | 1 |
| 19 | Capsule endoscopy in the evaluation of patientswith suspected small intestinal bleeding:results of a pilot study显示文摘 | Lewis BS Swain P | 2002 | Gastrointest Endosc2002，56，3： | 1 |
| 20 | Interactions between the Steinernema feltiae Xenorhabdus bovienii insect pathogen complex and the root- knot nematode Meloidogyne incognita显示文摘 | Lewis E E Grewal P S Sardanelli S | 2001 | Biological Control2001，21，： | 1 |
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