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| 1 | Cloning and Characterization of WOX4 Gene from Vitis vinifera L. Involved in Stem Cell Regulation显示文摘Wuschel-related homeobox (WOX) genes play essential, specific, and sometimes redundant roles in plant embryo development, shoot and root meristem maintenance, and plant development. Though much information was quickly gained with members of the WOX gene family of Arabidopsis, monocotyledonous crops, and gymnospermous conifers, little is known about perennial woody plants. In this study, we isolated the first WOX gene family member from grape (Vitis vinifera L. cv. Cabernet Sauvignon), and named it VvWOX4 based on its characteristic domains and phylogenetic analysis. The identity of VvWOX4 was validated by MALDI-TOF MS and Western blot with polyclonal antibody against Arabidopsis thaliana Wuschel. Functional analysis showed that VvWOX4 markedly increased shoot primordia structures when overexpressed under CaMV 35S promoter in tobacco. A different expression pattern was found for VvVOX4 compared with AtWUCHEL and its expression was detected in unique organs of grapevines. Besides the expression in the vegetative shoot apical meristem (SAM) of grape shoot tips, VvWOX4 is expressed in dormant winter buds, inflorescence, young leaves, and tendril tips, but not in root tips. In young leaves, the expression of VvWOX4 is strongly upregulated by wounding, and also by plant growth regulators such as 2 mg L-1 2,4-D, 1 mg L-1 NAA and 1 mg L-1 BAP treatments, while downregulation was monitored by 1 mg L-1 IBA treatment, and there was no response to 0.5 mg L-1 GA3 treatment. Together, our results revealed the first member of grape WOX gene family and indicated different roles and regulation of VvWOX4 in the perennial woody crop grapevine. | DAI Ru JIN Hai-peng WANG Zeng Avihai Perl XU Hai-ying ZHANG Wen CHEN Shang-wu MA Hui-qin | 2011 | Agricultural Sciences in China2011，10，12： | 3 |
| 2 | The Establishment of an Agrobacterium-Mediated Transformation Platform for the Non-Embryogenic Calli of Vitis vinifera L.显示文摘Non-embryogenic calli (NEC) was inevitably and heavily produced when grape embryogenic calli (EC) was induced from explants or during the subculture of EC.A stable and highly efficient NEC transformation platform is required to further sort out and verify key genes which determine/switch the identity of NEC and EC.In this research,a vector pA5 containing a chitinase signal sequence fused to gfp (green fluorescent protein) and an HDEL motive was used to target and immobilize into Agrobacterium strain EHA105 to establish a transformation platform for Vitis vinifera L.cv.Chardonnay NEC.It was determined that NEC 10 d after subculture was the best target tissue;30 min for inoculation followed by 3 d co-cultivation with the addition of 200 μmol L-1 acetosyringone (AS) was optimized as protocol.The use of bacterial densities as 1.0 at OD600 did not result in serious tissue hypersensitive reaction and it had higher efficiency.Kanamycin at 200 mg L-1 was picked for positive expression selection.The stable transformation of NEC was proved by reverse transcription-polymerase chain reaction techniques (RT-PCR) and fluorescent microscopy after three sub-cultures of the selected cell line.Highly efficient genetic transformation protocol of grape NEC was achieved and some of the optimized parameters were different from that reported for EC.This transformation platform could facilitate the verification of candidate somatic embryogenesis (SE) decisive genes,and the successfully transformed NEC with certain genes can also be used as bioreactors for the production of functional products,as NEC not only proliferates fast,but also keeps in a rather stable condition. | ZHAO Feng-xia CHEN Shang-wu Perl Avihai DAI Ru XU Hai-ying MA Hui-qin | 2011 | Agricultural Sciences in China2011，10，5： | 2 |
| 3 | Amp lification of HER22/neu gene in HER22/neu2overexp ressing and2nonexp ressing breast car2cinomas and their synchronous benign,p remalig-nant,and metastat2ic lesions detected by F ISH in archivalma-terial显示文摘 | Xu R Perle MA Inghirami G | 2002 | Mod Pathol2002，15，2： | 1 |
| 4 | The role of C5a in the in?nate immune response after experimental blunt chest trauma显示文摘 | Flierl MA Perl M Rittirsch D | 2008 | Shock2008，29，1： | 1 |
| 5 | Wilms' tumor in adults: aspiration cytology and cytogenetics显示文摘 | Li Peng Perle MA Scholes JV | 2001 | Diagn Cytopathol2001，26，： | 1 |
| 6 | The influence of finite three-dimensional multiple axial erosions on the fatigue life of partially autofrettaged pressurized cylinders显示文摘 | LEVY C PERL M MA Q | 2003 | Journal of Pressure Vessel Technology2003，125，4： | 1 |
| 7 | Amplification of HER - 2/ neu gene in HER - 2/neu - overexpressing and - nonexpressing breast carcinomas and their synchronous benign, premalignant, and metastatic lesions detected by FISH in archival material 显示文摘 | Xu R Perle MA Inghirami G | 2002 | Mod Pathol2002，15，2： | 1 |
| 8 | Isolated echogenic intraeardiac foei in patients with low-risk triple screen results: assessing the risk of trisomy 21显示文摘 | Koklanaris N Roman AS Perle MA | 2005 | J Perinat Med2005，33，6： | 1 |
| 9 | Erosions and their effect on the fatigue life of thick walled, autofrettaged, pressurized vessels显示文摘 | LEVY C PERL M MA Q | 2003 | Journal of Pressure Vessel Technology2003，125，3： | 1 |
| 10 | Amplification of HER-2/neu gene in HER-2/neu-overexpressing and -nonexpressing breast carcinomas and their synchronous benign,premalignant,and metastatic lesions detected by FISH in archival material显示文摘 | Xu R Perle MA Inghirami G | 2002 | Mod Pathol2002，15，2： | 1 |
| 11 | Chromosomal abnormalitiesand p53gene mutation in a cardiac angiosarcoma显示文摘 | Zu Y Perle MA Yan Z | 2001 | ApplImmunohistochem Mol Morphol2001，9，1： | 1 |
| 12 | Primary Ewing sarcoma/ PNET oft be kidney: fine-needle aspiration, histology, and dual eolor break apart FISH assay 显示文摘 | Kang SH Perle MA Nonaka D | 2007 | Diagn Cytopathol2007，35，6： | 1 |
| 13 | Let-7 repression leads to HMGA2 overexpression in uterine leiomyosarcoma显示文摘 | Shi G Perle MA Mittal K | | 0，，9： | 1 |
| 14 | Amplification of HER-2/ neu gene in HER - 2/neu - ore rexpressing and - nonexpressing breast carcinmas and their synchronous benign, premalignant, and metastatic lesions detected by FISH in archival material 显示文摘 | Xu R Perle MA Inghirami G | 2002 | Mod Patho12002，15，2： | 1 |
| 15 | The influence of finite three-dimensional multiple axial erosions on the fatigue life of partially autofrettaged pressurized cylinders显示文摘 | LEVY C PERL M MA Q | 2003 | Journal of Pressure Vessel Technology2003，125，4： | 1 |
| 16 | The role of C5a in the innate immune response after experimental blunt chest trauma显示文摘 | Flier1 MA Perl M Rittirsch D | 2008 | Shock2008，29，1： | 1 |
| 17 | Temporal effects of Sprouty on lung morphogenesis显示文摘 | Perl AK Hokuto I Impagnatiello MA | 2003 | Dev Biol2003，258，： | 1 |
| 18 | The Influence of Finite Three--dimensional Multiple Axial Erosions on the Fatigue Life of Partially Autofrettaged Pressurized Cylinders显示文摘 | Levy C Perl M Ma Q | 2003 | Journal of Pressure Vessel Technology2003，125，4： | 1 |
| 19 | Isolated echogenic intracardiac foci in patients with low-risk triple screen results : assessing the risk of trisomy 21 显示文摘 | Koklanaris N Roman AS Perle MA | 2005 | J Perinat Med2005，33，： | 1 |
| 20 | Isolated echogenic intracardiac foci in patients with low-risk triple screen results:assessing the risk of trisomy 21显示文摘 | Koklanaris N Roman AS Perle MA | 2005 | J PerinatMed2005，33，6： | 1 |
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