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| 1 | Diagnostic value of glypican-3 in serum and liver for primary hepatocellular carcinoma显示文摘AIM:To evaluate the diagnostic value of glypican-3(GPC3) in serum and liver for primary hepatocellular carcinoma(HCC).METHODS:Serum levels of GPC3 and α-fetoprotein(AFP) were measured in 75 patients with primary HCC and 32 patients with liver cirrhosis.Expression of GPC3 and AFP in 58 HCC and 12 cirrhotic specimens was detected with immunohistochemical staining.RESULTS:When the cut-off value of serum GPC3 was set at 300 ng/L,its sensitivity and specificity for HCC were 47.0% and 93.5%,respectively.Among the 14 patients with HCC at stage according to the Barcelona Clinic Liver Cancer staging system,the serum GPC3 level was higher than 300 ng/L in 50%(7/14) patients,the serum AFP level was not ≥ 400 μg/L in any patient.Combined serum AFP and GPC3 significantly increased the sensitivity to the diagnosis of HCC.The GPC3 expression was detected in cytoplasm of HCC cells but not in hepatocytes and bile ducts of benign tumors.Among the 58 HCC patients,the GPC3 was expressed in 100%(28/28) patients with their serum AFP level ≥ 400 μg/L,and in 90%(27/30) patients with their AFP level < 400 μg/L,respectively.The GPC3 was weakly or negatively expressed in all paracarcinomatous and cirrhotic tissue samples.AFP positive HCC cells were only found in 1 out of the 58 HCC patients.CONCLUSION:GPC3 protein is a sensitive and specific serum marker for diagnosis of early HCC.Its expression in liver tissues can be used to discriminate tumor cells from benign hepatic cells. | Hui Liu Li-Jie Zhang Peng Li Yun Zhai Yu-Fen Tan Ning Li Hui-Guo Ding Chun-Feng Qu | 2010 | World Journal of Gastroenterology2010，16，35： | 57 |
| 2 | Service-aware 6G:An intelligent and open network based on the convergence of communication,computing and caching显示文摘With the proliferation of the Internet of Things(IoT),various services are emerging with totally different features and requirements,which cannot be supported by the current fifth generation of mobile cellular networks(5G).The future sixth generation of mobile cellular networks(6G)is expected to have the capability to support new and unknown services with changing requirements.Hence,in addition to enhancing its capability by 10–100 times compared with 5G,6G should also be intelligent and open to adapt to the ever-changing services in the IoT,which requires a convergence of Communication,Computing and Caching(3C).Based on the analysis of the requirements of new services for 6G,this paper identifies key enabling technologies for an intelligent and open 6G network,all featured with 3C convergence.These technologies cover fundamental and emerging topics,including 3C-based spectrum management,radio channel construction,delay-aware transmission,wireless distributed computing,and network self-evolution.From the detailed analysis of these 3C-based technologies presented in this paper,we can see that although they are promising to enable an intelligent and open 6G,more efforts are needed to realize the expected 6G network. | Yiqing Zhou Ling Liu Lu Wang Ning Hui Xinyu Cui Jie Wu Yan Peng Yanli Qi Chengwen Xing | 2020 | Digital Communications and Networks2020，6，3： | 57 |
| 3 | Measurement of in-vehicle volatile organic compounds under static conditions显示文摘在车辆内的不稳定的有机化合物(VOC ) 的类型和数量用 theThermodesorber 气体在静态的条件下面在一新车辆和二旧车辆被决定了用色层法分离 / 集中分光计(TD-GC/MS ) 。空气采样和分析在 USEPA 方法 TO-17.A 房间尺寸的要求下面被进行,环境测试房间被利用提供要求的环境条件(温度,湿度,水平、垂直的气流速度,和背景 VOCsconcentration ) 的稳定、精确的控制。尽管在每车辆以内检测的全部的不稳定的有机化合物( TVOC )的数量是相对不同的,静态的车辆测试表明了那(在新车辆 A ,在使用的车辆 B 的 1240 亩 g/m^3 ,和在使用的车辆 C 的 132 ng/m^3 的 4940 u.g/m^3 ),甲苯,二甲苯,一些芳香族化合物,和各种各样的C_7-C_( 12 )链烷在在测试的所有三车辆的占优势的 VOC 种类之中。另外, tetramethyl succinonitrile,可能源于泡沫垫子在车辆 B.The 类型被检测, VOC 的数量根据因素的各种各样的类型更加变化了,例如,车辆年龄,车辆模型,温度,空气汇率,和环境气流速度。例如,如果气流速度从 0.1 m/s 增加到 0.7 m/s, vehicle'sair 汇率从 0.15 h~ 增加(-1)to 0.67 h^( 从 1780 ~ 1201 ng/m^3 的 -1),and 在里面车辆 TVOC 集中减少。 | YOU Ke-wei GE Yun-shan HU Bin NING Zhan-wu ZHAO Shou-tang ZHANG Yan-ni XIE Peng | 2007 | Journal of Environmental Sciences2007，19，10： | 27 |
| 4 | Elevated serum alpha fetoprotein levels promote pathological progression of hepatocellular carcinoma显示文摘AIM:To investigate the biological role of alpha fetoprotein (AFP) and its clinical signif icance in carcinogenesis of hepatocellular carcinoma (HCC).METHODS:Clinical analysis of HCC patients and im-munohistochemical examination were conducted to evaluate the relationship between serum AFP level and patient mortality. Confocal microscopy,Western blotting, dimethylthiahzolyl-2,5-diphenyl-tetrazolium bromide,Cell Counting Kit-8 assays and flow cytometry were performed to explore the possible mechanism.RESULTS: Among the 160 HCC patients enrolled in this study,130 patients survived 2 years (81.25%),with a survival rate of 86.8% in AFP < 2 0 μg/L group,88.9% in AFP 20-250 μg/L group,and 69.6% in AFP > 250 μg/L group, demonstrating a higher mortality rate in HCC patients with higher AFP levels. Surgical treatment was benef icial only in patients with low AFP levels.The mortality rate of HCC patients with high AFP levels who were treated surgically was apparently higher than those treated with conservative management.The results of immunohistochemistry showed that AFP and AFP receptor were merely expressed in tissues of HCC patients with positive serum AFP.Consistently,in vitro analysis showed that AFP and AFPS were expressed in HepG2 but not in HLE cells. AFP showed a capability to promote cell growth,and this was more apparent in HepG2 cells,in which the proliferation was increased by 3.5 folds. Cell cycle analysis showed that the percent-age of HepG2 cells in S phase after exposure to AFP was modestly increased.CONCLUSION:HCC patients with higher AFP levels show a higher mortality rate,which appears to be attributable to the growth promoting properties of AFP. | Peng Li Shan-Shan Wang Hui Liu Ning Li Michael A McNutt Gang Li Hui-Guo Ding | 2011 | World Journal of Gastroenterology2011，17，41： | 23 |
| 5 | Effect of Snow-cover Duration on Plant Species Diversity of Alpine Meadows on the Eastern Qinghai-Tibetan Plateau显示文摘Although snow cover plays an important role in structuring plant diversity in the alpine zone,there are few studies on the relationship between snow cover and species diversity of alpine meadows on the eastern Qinghai-Tibetan Plateau.To assess the effect of snow cover on plant species diversity of alpine meadows,we used ten parallel transects of 60m×1m for this study and described the changes in species diversity and composition associated with snow depth.With the division of snow depth into six classes,the highest species richness(S)and species diversity(H')occurred with an intermediate snow depth,i.e.,class III and class IV,showing a unimodal curve with the increase in snow depth.The relationship between snow depth and plant diversity(both richness and Shannon index)could be depicted by quadratic equations.There was no evident relationship between diversity(both S and H')and soil water content,which implied that other more important factors influenced species diversity.The patterns of diversity found in our study were largely attributed to freeze-thaw alteration,length of growing season and disturbances of livestock grazing.Furthermore,snow depth affected species composition,as evaluated by the Srensen's index of similarity.In addition,almost all species limited to one snow depth class were found only in class III and class IV,indicating that intermediate snow depth was suitable for the survival and growth of many alpine species. | CHEN Wennian WU Yan WU Ning LUO Peng | 2008 | Journal of Mountain Science2008，5，4： | 19 |
| 6 | Analysis of Dynamics and Driving Factors of Wetland Landscape in Zoige,Eastern Qinghai-Tibetan Plateau显示文摘Zoige Wetland is one of the largest plateau wetlands in the world.This paper provides a dynamic analysis of spatial and temporal patterns of the wetland in Zoige, Eastern Qinghai-Tibetan Plateau, supported by ERDAS8.7 and ArcGIS9.0.It is the first comparative analysis of a system of rapidly changing wetland with landscape patterns in Zoige, using 3 classified landsat Thematic Mapper images of 1977, 1994 and 2001.The classified images were used to generate wetland distributing maps, and shape index(S), diversity index(H), dominance index(D), evenness index(E), fragmentation index(F) and fractal dimension(Fd) were calculated and analyzed spatiotemporally across pure grazing area in Zoige for each landscape type and in different periods(before 1977, during 1977~1994 and 1994~2001), as well as the driving forces of natural and anthropogenic.The study shows that for a comprehensive understanding of the shapes and trajectories of the shrinking and desertificated land expansion of the wetland, a spatiotemporal landscape metrics analysis in different periods is an improvement than only with landscape changing rates.This type of analysis can also be used to infer underlying social, economic, and political processes that drive the observed wetland forms.The results indicate that wetland patterns can be changed over relatively short periods of time.Thetotal area of lake reduced by 164.86 km2,grassland extended by 141.74 km2,semi-marsh extended by 105.94 km2 , marsh reduced by 86.00 km2;the number of landscape patches reduced by 56, and their average area decreased by 2.68 km2;the successions within lake, marsh, semi-marsh and grassland were found obviously;S decreased stepwise;D and F increased but H decreased;The changing rate after 1994 was 2.3 to 2.9 times greater than that before.The change of the wetland landscape patterns resulted in the interaction between socio-economic and natural forces of positive and negative aspects;and natural factors affected as assistant aspect.Some important human activities in this period led to the change of the landscape patterns in this region directly.Some measurements made by government and NGO delayed the converting process partly. | QIU Pengfei WU Ning LUO Peng WANG Zuyuan LI Maihe | 2009 | Journal of Mountain Science2009，6，1： | 19 |
| 7 | Holocene high lake-levels and pan-lake period on Badain Jaran Desert显示文摘Many lakes exist in southeastern Badain Jaran Desert and its hinterland, including 110 perennial lakes and some seasonal or extinct lakes. Geomorphological, sedimentological, and bioglyph evidence obtained from field investigations on Badain Jaran Desert lake group, alongside measurements and dating performed on lake relic, prove that these lakes expanded while the climate was relatively wet during early and middle Holocene. The dating results suggest that the pan-lake period of the Badain Jaran Desert began at 10 cal kyr BP, before which the limnic peat period occurred(11–10 cal kyr BP). Many lakes reached their maximal water-level during 8.6–6.3 cal kyr BP and retreated or dried up in the late Holocene(about 3.5–0 cal kyr BP). During that period, the precipitation at Badain Jaran Desert may have reached 200 mm yr^(-1) for 7.7–5.3 cal kyr BP, inferred from both the age and precipitation rate of calcareous root tubes. The water balance calculation shows that wetter and warmer climate and the increase of underground water recharge were key factors in maintaining and developing the lake group at both centennial and millennial time scales. Furthermore, lake surface expansion and the increasing fresh water availability set the background for the prosperous prehistoric culture. | WANG NaiAng NING Kai LI ZhuoLun WANG YiXin JIA Peng MA Ling | 2016 | Science China Earth Sciences2016，59，8： | 19 |
| 8 | Salivary exosomal PSMA7: a promising biomarker of inflammatory bowel disease显示文摘煽动性的肠疾病(IBD ) 是包括中国在亚洲增加的流行的世界范围的肠的免疫者不正常的疾病。它是有未知原因的胃肠的道的长期的疾病。Exosomes 是在各种各样的身体液体的小泡。他们有 40-120 的直径 ? nm,并且他们的功能之一是各种各样的物质的长途的转移。在这研究,我们与 IBD 并且在健康控制在病人调查了唾液的 exosomes 的内容与 IBD 在病人探索新 biomarker。在这研究,整个口水与 IBD 从病人被获得(ulcerative 大肠炎(UC ) , n ?=? 37;Crohns 疾病(CD ) , n ?=? 11 ) 并且显然健康的个人(HC, n ?=? 10 ) 。唾液的 exosomes 从样品被提取,并且在 exosomes 以内的蛋白质被液体用色层法分离团分光计(LC-MS/MS ) 识别。结果证明超过 2000 蛋白质与 IBD 从病人在唾液的 exosomes 被检测。通过基因本体论分析,我们发现(PSMA7 ) proteasome 子单元 alpha 类型 7 与 IBD 和健康控制显示出病人之间的特别显著的差别,因为它的表达式水平在 CD 和 UC 组是高得多的。这 exosomal 蛋白质与 proteasome 活动和煽动性的回答有关。我们因此断定在这研究,唾液的 exosomal PSMA7 在在从有 IBD 的题目的唾液的 exosomes 的高水平是在场的。免除病人 colonoscopy 的疼痛能是很有希望的 biomarker。 | Xiaowen Zheng Feng Chen Qian Zhang Yulan Liu Peng You Shan Sun Jiuxiang Lin Ning Chen | 2017 | Protein & Cell2017，8，9： | 19 |
| 9 | Remote ischemic perconditioning prevents liver transplantation-induced ischemia/reperfusion injury in rats: Role of ROS/RNS and e NOS显示文摘AIM To investigate the underlying mechanisms of the protective role of remote ischemic perconditioning(RIPerC) in rat liver transplantation. METHODS Sprague-Dawley rats were subjected to sham, orthotopic liver transplantation(OLT), ischemic postconditioning(IPostC) or RIPerC. After 3 h reperfusion, blood samples were taken for measurement of alanine aminotransferase, aspartate aminotransferase, creatinine(Cr) and creatinine kinase-myocardial band(CK-MB). The liver lobes were harvested for the following measurements: reactive oxygen species(ROS), H2O2, mitochondrial membrane potential(ΔΨm) and total nitric oxide(NO). These measurements were determined using an ROS/H2O2, JC1 and Total NOx Assay Kit, respectively. Endothelial NO synthase(e NOS) was analyzed by reverse transcription-polymerase chain reaction(RTPCR) and western blotting, and peroxynitrite was semiquantified by western blotting of 3-nitrotyrosine. RESULTS Compared with the OLT group, the grafts subjected to RIPerC showed significantly improved liver and remote organ functions(P < 0.05). ROS(P < 0.001) including H2O2(P < 0.05) were largely elevated in the OLT group as compared with the sham group, and RIPerC(P < 0.05) reversed this trend. The collapse of ΔΨm induced by OLT ischemia/reperfusion(I/R) injury was significantly attenuated in the RIPerC group(P < 0.001). A marked increase of NO content and phosphoserine eN OS, both in protein and mR NA levels, was observed in liver graft of the RIPer C group as compared with the OLT group(P < 0.05). I/R-induced 3-nitrotyrosine content was significantly reduced in the RIPerC group as compared with the OLT group(P < 0.05). There were no significant differences between the RIPerC and IPostC groups for all the results except Cr. The Cr level was lower in the RIPerC group than in the IPostC group(P < 0.01).CONCLUSION Liver graft protection by RIPerC is similar to or better than that of IPostC, and involves inhibition of oxidative stress and up-regulation of the PI3K/Akt/e NOS/NO pathway. | Ning He Jun-Jun Jia Jian-Hui Li Yan-Fei Zhou Bing-Yi Lin Yi-Fan Peng Jun-Jie Chen Tian-Chi Chen Rong-Liang Tong Li Jiang Hai-Yang Xie Lin Zhou Shu-Sen Zheng | 2017 | World Journal of Gastroenterology2017，23，5： | 17 |
| 10 | Calcium channel blockers reduce severe fever with thrombocytopenia syndrome virus (SFTSV) related fatality显示文摘Severe fever with thrombocytopenia syndrome (SFTS),an emerging tick-borne infectious disease caused by a novel phlebovirus (SFTS virus, SFTSV), was listed among the top 10 priority infectious diseases by the World Health Organization due to its high fatality of 12%-50% and possibility of pandemic transmission. Currently, effective anti-SFTSV intervention remains unavailable. Here, by screening a library of FDA-approved drugs, we found that benidipine hydrochloride, a calcium channel blocker (CCB), inhibited SFTSV replication in vitro. Benidipine hydrochloride was revealed to inhibit virus infection through impairing virus internalization and genome replication. Further experiments showed that a broad panel of CCBs, including nifedipine, inhibited SFTSV infection. The anti-SFTSV effect of these two CCBs was further an a lyzed in a humanized mouse model in which CCB treatment resulted in reduced viral load and decreased fatality rate. Importantly, by performing a retrospective clinical investigation on a large cohort of 2087 SFTS patients, we revealed that nifedipine administration enhaneed virus clearance, improved clinical recovery, and remarkably reduced the case fatality rate by >5-fold. These findings are highly valuable for developing potential host-oriented therapeutics for SFTS and other lethal acute viral infections known to be inhibited by CCBs in vitro. | Hao Li Lei-Ke Zhang Shu-Fen Li Shao-Fei Zhang Wei-Wei Wan Yu-Lan Zhang Qi-Lin Xin Ke Dai Yuan-Yuan Hu Zhi-Bo Wang Xiang-Tao Zhu Yu-Jie Fang Ning Cui Pan-He Zhang Chun Yuan Qing-Bin Lu Jie-Ying Bai Fei Deng Geng-Fu Xiao Wei Liu Ke Peng | 2019 | Cell Research2019，29，9： | 15 |
| 11 | Size-dependent peroxidase-like catalytic activity of Fe_3O_4 nanoparticles显示文摘有三种不同尺寸的 Fe3O4 nanoparticles (NP ) 的象 Peroxidase 一样催化性质,由化学一起沉淀和大音阶的第五音鈥揼e l 方法综合了,被紫外鈥搗i s 光谱分析调查。由把 Fe3O4 NP 与 11, 20,和 150 nm 的平均直径作比较,我们发现催化活动与减少的 nanoparticle 尺寸增加。用反应产品和底层的反应水流描绘 Fe3O4 NP 的催化活动的电气化学的方法也被开发。 | Fang Fang Peng YU Zhang Ning Gu | 2008 | Chinese Chemical Letters2008，19，6： | 14 |
| 12 | Generation of male germ cells from induced pluripotent stem cells (iPS cells): an in vitro and in vivo study显示文摘最近的研究报导了从老鼠和人的房间能区分进初发的细菌房间的那导致的 pluripotent 茎(iPS ) 。然而, iPS 房间是否能够生产男细菌房间,不被知道。这研究的目的进 spermatogonial 干细胞和迟了阶段的男细菌房间的老鼠 iPS 房间是调查区别潜在的。我们使用了在 vitro 区别并且在 vivo 移植联合的一条途径。胚胎植物或动物身体(EB ) 用白血球过多症禁止者因素(LIF ) 从 iPS 房间被获得免费媒介。量的 PCR 表明在 Oct4 表示的减少和 Stra8 的增加和在 EB 的 Vasa mRNA 源于 iPS 房间。iPS 导出房间的 EB 被区分进 spermatogonial 干细胞(SSC ) ,,的酸由他们 VASA,以及 CDH1 和 GFRα 的表示证实了的 retinoic 导致; 1,它是 SSC 的标记。而且,从 iPS 房间导出的这些细菌房间被移植进与 busulfan 被预先对待的老鼠的接受者睾丸。尤其是, iPS 导出房间的 SSC 能区分进从 spermatogonia 到圆 spermatids,由 VASA 和 SCP3 表示出现的男细菌房间。这研究证明 iPS 房间有潜力区分进迟了阶段的男细菌房间。从 iPS 房间的男细菌房间的推导在男不孕的处理有潜在的应用程序并且为揭开提供一个模型位于男细菌房间开发下面的分子的机制。 | Yong Zhu Hong-Liang Hu Peng Li Shi Yang Wei Zhang Hui Ding Ru-Hui Tian Ye Ning Ling-Ling Zhang Xi-Zhi Guo Zhan-Ping Shi Zheng Li Zuping He | 2012 | Asian Journal of Andrology2012，14，4： | 13 |
| 13 | Growth and Common Prosperity in China显示文摘This paper introduces a decomposition method that quantifies the contributions to common prosperity of labor market performance and social policies and extends the idea of shared prosperity to a new measure of inequity in opportunities.The resulting common prosperity indices and opportunity equality indices are then applied to five waves of the Chinese Household Income Project data from 1988 to 2018.This paper shows that the labor market performance and social policies have been improving over the last 30 years and have helped China move towards common prosperity for everyone.The indices developed in this paper allow us to quantify the extent of shared prosperity that a country has achieved and to carry out empirical studies on which policy is working and which is not.It can also help us identify the fundamental causes of inequality and aid us in achieving equality in opportunity among all members of society. | Nanak Kahwani Xiaobing Wang Ning Xue Peng Zhan | 2022 | China & World Economy2022，30，1： | 13 |
| 14 | Biphasic effects of sodium danshensu on vesse function in isolated rat aorta显示文摘 | Ning ZHANG Hao ZOU Lei JIN Jian WANG Mei-fang ZHONG Peng HUANG Bing-qing GU Shi-long MAO Chuan ZHANG Hong CHEN | 2010 | Acta Pharmacologica Sinica2010，31，4： | 13 |
| 15 | Dapper1 promotes autophagy by enhancing the Beclinl -Vps34-Atg14L complex formation显示文摘 | Benvu Ma Weipeng Cao Wenxia Li Chan Gao Zhen Qi Yan Zhao Jun Du Hua Xue Junya Peng Jun Wen Hua Chen Yuanheng Ning Lei Huang Hong Zhang Xiang Gao Li Yu Ye-Guang Chen | 2014 | Cell Research2014，24，8： | 12 |
| 16 | Effect of hydrophilic silica nanoparticles on hydrate formation: Insight from the experimental study显示文摘Invasion of drilling fluid into natural gas hydrate deposits during drilling might damage the reservoir,induce hydrate dissociation and then cause wellbore instability and distortion of the data from well logging. Adding nanoparticles into drilling fluid is an effective method in reducing the invasion of drilling fluid and enhancing borehole stability. However, the addition of nanoparticles might also introduce hydrate formation risk in borehole because they can act as the 'seeds' for hydrate nucleation. This paper presents an experimental study of the effect of hydrophilic silica nanoparticle on gas hydrate formation in a dynamic methane/liquid-water system. In the experiment, the ultrapure water with and without1.0 wt%–6.0 wt% concentrations of silica nanoparticles, grain sizes of 20 and 50 nm, were pressurized by methane gas under varied conditions of temperature and pressure. The induction time, the gas consumption, and the average rate of gas consumption in the system were measured and compared to those in ultrapure water. The results show that a concentration of 4.0 wt% hydrophilic SiO_2 particles with a grain size of 50 nm has a relatively strong inhibition effect on hydrate formation when the initial experimental condition is 5.0 °C and 5.0 MPa. Compared to ultrapure water, the hydrophilic nano-SiO_2 fluid increases the induction time for hydrate formation by 194% and decreases the amount and average rate of hydrate formation by 10% and 17%, respectively. This inhibition effect may be attributed to the hydrophilicity,amount and aggregation of silica nanoparticle according to the results of water activity and zeta potential measurements. Our work also elucidates hydrophilic, instead of hydrophobic, nanoparticles can be added to the drilling fluid to maintain wellbore stability and to protect the hydrate reservoir from drilling mud damage, because they exhibit certain degree of hydrate inhibition which can reduce the risk of hydrate reformation and aggregation during gas hydrate or deep water drilling if their concentration can be controlled properly. | Ren Wang Tianle Liu Fulong Ning Wenjia Ou Ling Zhang Zhen Wang Li Peng Jiaxin Sun Zhichao Liu Tianshu Li Huicui Sun Guosheng Jiang | 2019 | Journal of Energy Chemistry2019，28，3： | 12 |
| 17 | Exosome-functionalized polyetheretherketone-based implant with immunomodulatory property for enhancing osseointegration显示文摘The host immune response effecting on biomaterials is critical to determine implant fates and bone regeneration property.Bone marrow stem cells(BMSCs)derived exosomes(Exos)contain multiple biosignal molecules and have been demonstrated to exhibit immunomodulatory functions.Herein,we develop a BMSC-derived Exos-functionalized implant to accelerate bone integration by immunoregulation.BMSC-derived Exos were reversibly incorporated on tannic acid(TA)modified sulfonated polyetheretherketone(SPEEK)via the strong interaction of TA with biomacromolecules.The slowly released Exos from SPEEK can be phagocytosed by co-cultured cells,which could efficiently improve the biocompatibilities of SPEEK.In vitro results showed the Exos loaded SPEEK promoted macrophage M2 polarization via the NF-κB pathway to enhance BMSCs osteogenic differentiation.Further in vivo rat air-pouch model and rat femoral drilling model assessment of Exos loaded SPEEK revealed efficient macrophage M2 polarization,desirable new bone formation,and satisfactory osseointegration.Thus,BMSC-derived Exos-functionalized implant exerted osteoimmunomodulation effect to promote osteogenesis. | Lei Fan Pengfei Guan Cairong Xiao Huiquan Wen Qiyou Wang Can Liu Yian Luo Limin Ma Guoxin Tan Peng Yu Lei Zhou Chengyun Ning | 2021 | Bioactive Materials2021，6，9： | 11 |
| 18 | Optimal in vitro culture conditions for murine predominant immature CD8a^+ dendritic cells显示文摘背景使用树枝状的房间(DC2 ) 移植建立的不成熟的 CD8a+ 的前景为在未来的自体免疫的疾病的 immunologic 忍耐和处理是有希望的。然而,为导致 DC2 的方法仍然正在被探索。现在的学习被瞄准调查处于为准备大数字 f 的 vitro 条件最佳从鼠科的骨头髓 cells.Methods 的占优势的 DC2 在不同条件下面有教养的三组骨头髓房间被检验,也就是一个 导致cytokine 的试验性的组( cytokine 组),有刺激因素的granulocyte巨噬细胞殖民地的低集中的一个控制组( GM-CSF ,低 GM-CSF 组)并且没有 ndogenous cytokines 的一个控制组。cytokine 组与 5 ng/ml GM-CSF 是有教养的, 25 ng/ml Fit3 ligand (Flt3L ) , 20 ng/ml interleukin 4 (IL-4 ) 并且 100 ng/ml 干细胞因素(SCF ) 。低 GM-CSF 控制组与 0.4 ng/ml GM-CSF, 25 ng/ml FIt3L 和 100 ng/ml SCF 是有教养的,没有 IL-4。没有 dditional cytokines,没有外长的 cytokines 的控制组是有教养的。所有房间在 5% CO2 下面在 37 点是有教养的。在天 3, 7 和 16, 4 颜色流动 cytometry 被执行分析房间显型,和全部的房间数字被认为分析房间收益。阶段对比显微镜学被用来观察到 cytokine 组更高展出了的房间 morphologies.Results 分配 f 典型不成熟的 CD8a+ DC 特别在白天 3 上,但是全部的房间数字和 DC2 比例在延长文化期间减少了。低 GM-CSF 控制组在天显示出象 cytokine 组的一样的趋势 16 和 22,但是生产更高全部的房间数字(P < 0.05 ) 与更低的 DC2 比例和房间数字。没有外长的 cytokines 的控制组自发地产生了 DC2 的某个比例,但是与低全部的房间和 DC2 数字,那很快减少了,特别在延长文化期间(天 7 和 16 , P < 0.05 ) .Conclusions 文化面对 5 ng/ml GM-CSF , 25 ng/ml Flt3L , 20 ng/ml IL-4 和 100 ng/ml SCF 能很快从鼠科的骨头髓房间导致占优势的不成熟的CD8a+ DC 的大数量。因此,这些为在 vitro 准备鼠科的不成熟的 DC2 代表最佳的文化条件。 | NA Ning XU Lin CAO Kai-yuan LUO Yun YUAN Guang-qing XIANG Peng HONG Liang-qing LI Shu-nong | 2009 | Chinese Medical Journal2009，，3： | 11 |
| 19 | Characteristics of molecular weight distribution of dissolved organic matter in bromide-containing water and disinfection by-product formation properties during treatment processes显示文摘The characteristics of dissolved organic matter(DOM) and bromide ion concentration have a significant influence on the formation of disinfection by-products(DBPs). In order to identify the main DBP precursors, DOM was divided into five fractions based on molecular weight(MW), trihalomethane formation potential and haloacetic acid formation potential were determined for fractions, and the change in contents of different fractions and total DBPs during treatment processes(pre-chlorination, coagulation, sand filtration,disinfection) were studied. Moreover, the relationship between bromide concentration and DBP generation characteristics in processes was also analyzed. The results showed that the main DBP precursors were the fraction with MW < 1 k Da and fraction with MW 3-10 k Da, and the DBP's generation ability of lower molecular weight DOM(< 10 k Da) was higher than that of higher molecular weight DOM. During different processes,pre-chlorination and disinfection had limited effect on removing organics but could alter the MW distribution, and coagulation and filtration could effectively remove organics with higher MW. For DBPs, trihalomethanes(THMs) were mainly generated in pre-chlorination and disinfection, while haloacetic acids(HAAs) were mostly generated during pre-chlorination; coagulation and sand filtration had little effect on THMs but resulted in a slight removal of HAAs. In addition, the results of ANOVA tests suggested that molecular sizes and treatment processes have significant influence on DBP formation. With increasing bromide concentration, the brominated DBPs significantly increased, but the bromine incorporation factor in the processes was basically consistent at each concentration. | Ying Zhang Ning Zhang Peng Zhao Zhiguang Niu | 2018 | Journal of Environmental Sciences2018，30，3： | 11 |
| 20 | Diffusion kurtosis imaging: correlation analysis of quantitative model parameters with molecular features in advanced lung adenocarcinoma显示文摘Background:Due to development of magnetic resonance-based functional imaging, it is easier to detect micro-structural alterations of tumor tissues. The aim of this study was to conduct a preliminary evaluation of the correlation of non-Gaussian diffusion kurtosis imaging (DKI) parameters with expression of molecular markers (epidermal growth factor receptor [ EGFR];anaplastic lymphoma kinase [ ALK];Ki-67 protein) in patients with advanced lung adenocarcinoma, using routine diffusion-weighted imaging as the reference standard. Methods::Data from patients with primary lung adenocarcinoma diagnosed at Cancer Hospital, Chinese Academy of Medical Sciences (CHCAMS) from 2016 to 2019 were collected for retrospective analysis. The pathologic and magnetic resonance imaging data of 96 patients who met the inclusion criteria were included in this study. Specifically, the Kapp and Dapp parameters measured from the DKI model;apparent diffusion coefficient (ADC) value from the diffusion-weighted imaging model;and the EGFR, ALK, and Ki-67 biomarkers detected by immunohistochemistry and/or molecular biology techniques after biopsy or surgery were evaluated. The relations between quantitative parameters (ADC, Kapp, Dapp) and pathologic outcomes ( EGFR, ALK, and Ki-67 expression) were analyzed by Spearman correlation test. Results:Of the 96 lung adenocarcinoma lesions (from 96 patients), the number of EGFR- and ALK-positive and high Ki-67 expressing lesions were 53, 12, and 83, respectively. The Kapp values were significantly higher among patients with EGFR-positive mutations (0.81 ± 0.12 vs. 0.66 ± 0.10, t = 6.41, P < 0.001), ALK rearrangement-negative (0.76 ± 0.12 vs. 0.60 ± 0.15, t = 4.09, P < 0.001), and high Ki-67 proliferative index (PI) (0.76 ± 0.12 vs. 0.58 ± 0.13, t = 4.88, P < 0.001). The Dapp values were significantly lower among patients with high Ki-67 PI (3.19 ± 0.69 μm 2/ms vs. 4.20 ± 0.83 μm 2/ms, t = 4.80, P < 0.001) and EGFR-positive mutations (3.11 ± 0.73 μm 2/ms vs. 3.59 ± 0.77 μm ^2/ms, t = 3.12, P = 0.002). The differences in mean Dapp (3.73 ± 1.26 μm^ 2/ms vs. 3.26 ± 0.68 μm 2/ms, t = 1.96, P = 0.053) or ADC values ([1.34 ± 0.81] × 10^ -3 mm ^2/s vs. [1.33 ± 0.41] × 10 ^-3 mm ^2/s, t = 0.07, P = 0.941) between the groups with or without ALK rearrangements were not statistically significant. The ADC values were significantly lower among patients with EGFR-positive mutation ([1.19 ± 0.37] × 10 ^-3 mm^ 2/s vs. [1.50 ± 0.53] × 10 ^-3 mm ^2/s, t = 3.38, P = 0.001) and high Ki-67 PI ([1.28 ± 0.39] × 10 -3 mm 2/s vs. [1.67 ± 0.77] × 10^ -3 mm^ 2/s, t = 2.88, P = 0.005). Kapp was strongly positively correlated with EGFR mutations ( r = 0.844, P = 0.008), strongly positively correlated with Ki-67 PI ( r = 0.882, P = 0.001), and strongly negatively correlated with ALK rearrangements ( r = -0.772, P = 0.001). Dapp was moderately correlated with EGFR mutations ( r = -0.650, P = 0.024) or Ki-67 PI ( r = -0.734, P = 0.012). ADC was moderately correlated with Ki-67 PI ( r = -0.679, P = 0.033). Conclusions:The Kapp value of DKI parameters was strongly correlated with different expression of EGFR, ALK, and Ki-67 in advanced lung adenocarcinoma. The results potentially indicate a surrogate measure of the status of different molecular markers assessed by non-invasive imaging tools. | Qin Peng Wei Tang Yao Huang Ning Wu Lin Yang Ni Li | 2020 | Chinese Medical Journal2020，，20： | 11 |
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