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| 1 | Properties of serine:glyoxylate aminotransferase purified from Arabidopsis thaliana leaves显示文摘相片呼吸的酶 L-serine:glyoxylate amino-transferase (SGAT;EC 2.6.1.45 ) 从 Arabidopsis thaliana 叶子被净化。最后的酶是约 80% 作为纯与银染色由钠 dodecyl sulfate-polyacrylamide 凝胶电泳揭示了。酶的身份被 LC/MS/MS 分析证实。分别地,凝胶过滤层析在非使中毒的条件,集体 spectrometry (飞行技术的帮助矩阵的激光解吸附作用 / 电离 / 时间) 和钠 dodecyl sulfate-polyacrylamide 凝胶电泳下面在 Sephadex G-150 上估计的分子的团是 82.4 kDa, 42.0 kDa,和 39.8 kDa 显示象活跃形式更暗淡。最佳 pH 价值是 9.2。酶活动被 aminooxyacetate 禁止, beta-chloro-L-alanine 两个都加重与 pyridoxal 的羰基组一起反应。酶有 L 丙氨酸和 glyoxylate 的 transaminating 活动作为底层是与 L 丝氨酸和 glyoxylate 观察了那中的约 55% 个。更低的 K-m 价值( 1.25 公里) f 或L丙氨酸,比 k (猫) /K-m (重量的单位)建议那高与另外的植物 SGAT ,和 k (猫) /K-m (翼)比率的相比是近似2褶层在相片呼吸期间,翼和重量的单位被 Arabidopsis SGAT 为 glyoxylate 与相等的效率用作氨基的组施主。平衡常数(K-eq ) ,源于霍尔丹关系因为在有 hydroxypyruvate 和甘氨酸的形成的 L 丝氨酸和 glyoxylate 之间的 transamination 反应是 79.1,强烈赞成甘氨酸合成。然而,它被 2.83 公里的低 K-m 价值为甘氨酸伴随。有 recombinant Arabidopsis SGAT 的学习的酶的一些运动性质的比较以前获得了揭示实质的差别。有 L 丙氨酸和 glyoxylate 的 transamination 反应的速度的比率作为有 L 丝氨酸和 glyoxylate 的底层对那是为本国的酶的 1:1.8,而它是为 recombinant SGAT 的 1:7。本国的 SGAT 与 recombinant 酶相比为 L 丙氨酸显示出低得多的 K-m 价值。 | Maria Kendziorek Andrzej Paszkowski | 2008 | Acta Biochimica et Biophysica Sinica2008,40,2: | 2 |
| 2 | Therapeutic Application of Caspase 1/Interleukin-1β-Converting Enzyme Inhibitor Decreases the Death Rate in Severe Acute Experimental Pancreatitis显示文摘 | Adam S. Paszkowski Bettina Rau Jens M. Mayer Peter M?ller Hans G. Beger | 2002 | Annals of Surgery2002,,1: | 2 |
| 3 | Clinical relevance of caspase-1 activated cytokines in acute pancreatitis: High correlation of serum interleukin-18 with pancreatic necrosis and systemic complications显示文摘 | Bettina Rau Katja Baumgart Adam S. Paszkowski Jens M. Mayer Hans G. Beger | 2001 | Critical Care Medicine2001,,8: | 2 |
| 4 | Genetic diversity for mycorrhizal symbiosis and phosphate transporters in rice显示文摘Phosphorus(P) is a major plant nutrient and developing crops with higher P-use efficiency is an important breeding goal.In this context we have conducted a comparative study of irrigated and rainfed rice varieties to assess genotypic differences in colonization with arbuscular mycorrhizal(AM) fungi and expression of different P transporter genes.Plants were grown in three different soil samples from a rice farm in the Philippines.The data show that AM symbiosis in all varieties was established after 4 weeks of growth under aerobic conditions and that,in soil derived from a rice paddy,natural AM populations recovered within6 weeks.The analysis of AM marker genes(AM1,AM3,AM14) and P transporter genes for the direct Pi uptake(PT2,PT6) and AM-mediated pathway(PT11,PT13) were largely in agreement with the observed root AM colonization providing a useful tool for diversity studies.Interestingly,delayed AM colonization was observed in the aus-type rice varieties which might be due to their different root structure and might confer an advantage for weed competition in the field.The data further showed that P-starvation induced root growth and expression of the high-affinity P transporter PT6 was highest in the irrigated variety IR66 which also maintained grain yield under P-deficient field conditions. | Kwanho Jeong Nicolas Mattes Sheryl Catausan Joong Hyoun Chin Uta Paszkowski Sigrid Heuer | 2015 | Journal of Integrative Plant Biology2015,57,11: | 2 |
| 5 | Change in ploidy can modify epigenetic silencing显示文摘 | Scheid O M Jakovleva L Afsar K Maluszynska J Paszkowski J A | 1996 | Proc Natl Acad Sci USA1996,93,71: | 2 |
| 6 | Women in dental surgeries: reproductive hazards in occupational exposure to metallic mercury显示文摘 | Radzislaw Sikorski Teodor Juszkiewicz Tomasz Paszkowski Teresa Szprengier-Juszkiewicz | 1987 | International Archives of Occupational and Environmental Health1987,,6: | 2 |
| 7 | Cytosine methylation at CG and CNG sites is not a prerequisite for the initiation of transcriptional gene silencing in plants,but it is required for its maintenance显示文摘 | DIEGUEZ M J VAUCHERET H PASZKOWSKI J SCHEID O M | 1998 | Molecular & General Genetics1998,259,2: | 1 |
| 8 | Gene targeting in plants 显示文摘 | Paszkowski J Baur M Bogucki A | 1988 | EMBO J1988,7,13: | 1 |
| 9 | Therapeutic application of Caspase-1/interleukin-1βconverting enzyme inhibitor decreases the death rate in severe acute experimental pancreatitis 显示文摘 | Rau B Mayer JM | 2002 | Ann Surg2002,235,1: | 1 |
| 10 | Clinical relevance of caspase1 activated cytokines in acute pancreatitis: High correlation of serum interkeukin-18 with pancreatic necrosis and systemic complications 显示文摘 | Baumagrt K Paszkowski AS | 2001 | Crit Care Med2001,29,8: | 1 |
| 11 | Differential effects of caspase-1/interleukin-1beta-converting enzyme on acinar cell necrosis and apoptosis in severe acute experimental pancreatitis 显示文摘 | Paszkowski A Lillich S | 2001 | Lab Invest2001,81,7: | 1 |
| 12 | Rice phosphate transporters include an envolutionarily divergent gene specifically activates in arbuscular mycorrhizal symbiosis显示文摘 | Paszkowski U Kroken S Roux C | 2002 | Proceedings of the National Academy of Science USA2002,99,13: | 1 |
| 13 | Therapeutic application of Caspase l/interleukin-lbeta-converting enzyme inhibitor decreases the death rate in severe acute experimental pancreatitis 显示文摘 | Paszkowski AS Rau B Mayer JM | 2002 | Ann Surg2002,235,1: | 1 |
| 14 | Anti-ICAM-1 antibody modulates late onset of acinar cell apoptosis and early necrosis in taurocholate-induced experimental acute pancreatitis显示文摘 | Rau B Paszkowski A Esber S | | 0,,: | 1 |
| 15 | Clinical relevance of caspase-1 activated cytokines in acute pancreatitis:high correlationof serum interleukin-18 with pancreatic necrosis and systemic complications 显示文摘 | Ran B Banmgart K Paszkowski AS | 2001 | Crit Care Med2001,29,8: | 1 |
| 16 | Cytokine re- sponse in the postoperative period after surgical treatment of benign adnexal masses: comparison between laparoscopy and laparotomy显示文摘 | TORRES A TORRESN K PASZKOWSKI T | 2007 | SurgEndosc2007,21,10: | 1 |
| 17 | Weights in the balance: jas- monic acid and salicylic acid signaling in root - biotroph interac- tions显示文摘 | Caroline Gutjahr Uta Paszkowski | 2009 | Molecular Plant - Microbe Interactions2009,22,: | 1 |
| 18 | Functioning of they-aminobutyrate pathway in wheat seedlings affected byosmotic stress 显示文摘 | Bartyzel I Pelczar K Paszkowski A | 2003 | Biologia Plantarum2003,47,2: | 1 |
| 19 | Maintenance ol CpG methylation is essential for epigenetic inheritance during plant gametogenesis显示文摘 | SAZE H MITTELSTEN SCHEID O PASZKOWSKI J | 2003 | Nat Genet2003,34,1: | 1 |
| 20 | DNA and histone methylation in plants 显示文摘 | Tariq M Paszkowski J | 2004 | Trends in Genetics2004,20,6: | 1 |