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| 1 | A comparative study of the ovicidal and larvicidal activities of aqueous and ethanolic extracts of pawpaw seeds Carica papaya(Caricaceae) on Heligmosomoides bakeri显示文摘Objective:To assess the ovicidal and larvicidal activities of aqueous and ethanolic extracts of pawpaw seeds Carica papaya(Caricaceae) on the eggs and first stage larvae(L1) of Heligmosomoides bakeri.Methods:Eggs of this parasite were obtained from experimentally infested mice(Mus musculus) and larvae were from eggs after incubation at 25℃for about 72 hours.The eggs and larvae were exposed to ten different concentrations(0.125,0.25,0.375,0.5, 0.75,1.0,1.25,1.75,2.25 and 2.75 mg/mL) of both aqueous and ethanolic extracts respectively for 72 hours.Distilled water and 0.05%ethanol used as placebo and negative control,respectively. Results:Placebo and negative control group all showed average 92%embryonnation,98%egg hatching and 2%larval mortality,and did not affect development and larval survival.The extracts inhibited embryonic development,egg hatching and larval survival.In general,the ovicidal and larvicidal activities increased with increasing concentration of different extracts.The aqueous extract was found to be more potent on eggs than on larvae.At 2.75 mg/mL,only 8%of eggs embryonnated and 50%hatched to L1 vs 57%embryonic development and 79%hatching occurred in the ethanolic extract.However,this later extract was more efficient in preventing larval development producing 96%mortality as against 68%with the aqueous extract.Conclusions: These results shows the ovicidal and larvicidal properties of aqueous and ethanolic pawpaw seeds extracts. | Wabo Pone J Ngankam Ntemah JD Bilong Bilong CF Mpoame Mbida | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，6： | 3 |
| 2 | In vitro activities of acetonic extracts from leaves of three forage legumes(Calliandra calotyrsus,Gliricidia sepium and Leucaena diversifolia) on Haemonchus contortus显示文摘Objective:To assess ovicidal activity of three acetonic extracts from the leaves of three forage legume,Calliandra calotyrsus(C.calotyrsus),Gliricidia sepium(G.sepium) and Leucaena diversifolia(L.diversifolia) in vitro on Haemonchus contortus(H.contortus).Methods:Eggs were exposed for 24 hours to five different concentrations(0.075,0.15,0.3,0.6 and 1.25 mg/mL) of acetonic extracts at room temperature(24℃).Distilled water and 0.4%Tween were used in the bioassay as negative controls.Results:The later did not affect embryonation and egg hatching of H.contortus.Conversively,significant effects were obtained with the acetonic extracts of leaves of all three plants and the maximum activity was observed with the highest concentration (1.25 mg/mL).The acetonic extract of G.sepium was found to be more active(2.9%and 0.0% for embryonation and egg hatching,respectively) than the other substances 16.5%and 33.5%, respectively for C.calothyrsus,33.7%and 33.3%,respectively for L diversifolia.Conclusions: These results suggest that the three forage legumes do possess ovicidal properties and further studies on larvae should be carried out. | Wabo Pone J Kenne Tameli Florence Mpoame Mbida Pamo Tedonkeng E Bilong Bilong CF | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，2： | 2 |
| 3 | The use of human skin recombinants as an in vitro model for testing the irritation potential of cutaneous irritants 显示文摘 | Ponee M Kempenaar J | 1995 | Skin Pharmacol1995，8，： | 1 |
| 4 | The spontaneous combustion of coal and its by - products in the Witbank and Sasolburg coalfields of South Africa 显示文摘 | Pone J D Hein K A | 2007 | International Journal of Coal Geology2007，，： | 1 |
| 5 | Rrsponses of mixedfunction oxygenase and antioxdase enzyme system of Mytilus sp to organic pollution 显示文摘 | PONE C SOLE M LBAIGES J A | 1991 | Comp Biochem Physiol1991，100，12： | 1 |
| 6 | The spontaneous combustion of coal and its by-products in the Witbank and Sasolburg coalfields of South Africa显示文摘 | Denis J N Pone Kim A AHein Glenn B Stratcher | 2007 | International Journal of Coal Geology2007，72，： | 1 |
| 7 | 显示文摘 | Ferreira A E N Ponees Freire A M J Volt E O | 2003 | Biochemical2003，376，1： | 1 |
| 8 | Three - dimensional carbon dioxide - induced strain distribution within a confined bituminous coal显示文摘 | J Denis N Pone Michael Hile Phillip M Halleck Jonathan P Mathews | 2009 | Intemati - onal Journal of Coal Geology2009，77，10： | 1 |
| 9 | IgG subclasses in children with recurrent respiratory diseases显示文摘 | Kapellerova Kasanicra A Ponee J | 1993 | Cesk Pediatr1993，48，12： | 1 |
| 10 | BCR-signalling synergizes with TLR-signalling for induction of AID and immunoglobulin class-switching through the non-canonical NF-κB pathway显示文摘 | Pone EJ Zhang J Mai T | | 0，，： | 1 |
| 11 | B cell TLRs and induction of immunoglobulin class-switch DNA recombination显示文摘 | Pone E J Xu Z White CA | | 0，，： | 1 |
| 12 | Comparison of red chilli (Capsicum annuum) oleoresin and astaxanthin on rainbow trout ( Oncorhynchus mykiss ) fillet pigmentation 显示文摘 | Ingle G Arredondo J L Ponee J T | 2006 | Aquaculture2006，258，： | 1 |
| 13 | High-resolution X-ray computed tomography observations of the thermal drying of lump-sized subbituminous coal显示文摘 | MATHEWS J P PONE J D N MITCHELL G D | 2011 | Fuel Processing Technology2011，92，： | 1 |
| 14 | Phytase enzymology,applications,and biotechnology显示文摘 | LeiX G Pones J M | 2003 | Biotechnol Lett2003，25，21： | 1 |
| 15 | Systematic review of enhanced recovery pregrammes in colonic surgery 显示文摘 | Wind J Pone SW Fung Kon Jin PH | 2006 | Br J Surg2006，93，7： | 1 |
| 16 | The spontaneous combustion of coal and its by-products in the Witbank and Sasolburg coalfields of South Africa显示文摘 | PONE J D N HEIN K A A STRATCHER G B | 2007 | International Journal of Coal Geology2007，72，2： | 1 |
| 17 | Chronically inflamed hu- man tissues are infiltrated by highly differentiated Thl7 lympho- cytes 显示文摘 | POne J Chevalier S Preisser L | 2008 | J Immunol2008，180，11： | 1 |
| 18 | The spontaneous combustion of coal and its byproducts in the Witbank and Sasolburg coalfields of South Africa显示文摘 | PONE J D N HEIN K A A STRACHER G B | 2007 | International Journal of Coal Geology2007，72，： | 1 |
| 19 | Mesenchymal stem cells inhibit human Thl7 cell differentiation and function and induce a Tregulatory cell phenotype显示文摘 | Ghannam S POne J Forcy-Moquet G | 2010 | J Immunol2010，185，： | 1 |
| 20 | Inflamma- tion and Carotid AJlery - Risk for Atherosclerosis study (ICARAS) 显示文摘 | Schillinger M Exner M Mlekusch W Sabeti S Amighi J Nikowitsch R Timmel E Kickinger B Minar C Pones M Lalouschek W Rumpold H Maurer G Wagner O Minar E | 2005 | Circulation2005，111，17： | 1 |
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