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| 1 | Interleukin-1βinduces human cementoblasts to support osteoclastogenesis显示文摘Injury of the periodontium followed by inflammatory response often leads to root resorption. Resorption is accomplished by osteoclasts and their generation may depend on an interaction with the cells in direct contact with the root, the cementoblasts.Our study aimed to investigate the role of human cementoblasts in the formation of osteoclasts and the effect of interleukin(IL)-1β hereupon. Extracted teeth from healthy volunteers were subjected to sequential digestion by type I collagenase and trypsin.The effect of enzymatic digestion on the presence of cells on the root surface was analyzed by histology. Gene expression of primary human cementoblasts(p HCB) was compared with a human cementoblast cell line(HCEM). The p HCBs were analyzed for their expression of IL-1 receptors as well as of receptor activator of nuclear factor kappa-B ligand(RANKL) and osteoprotegerin(OPG). In a co-culture system consisting of osteoclast precursors(blood monocytes) and p HCBs, the formation of osteoclasts and their resorptive activity was assessed by osteo-assay and ivory slices. The cells obtained after a 120 min enzyme digestion expressed the highest level of bone sialoprotein, similar to that of HCEM. This fraction of isolated cells also shared a similar expression pattern of IL-1 receptors(IL1-R1 and IL1-R2). Treatment with IL-1β potently upregulated RANKL expression but not of OPG. p HCBs were shown to induce the formation of functional osteoclasts. This capacity was significantly stimulated by pretreating the p HCBs with IL-1β prior to their co-culture with human blood monocytes. Our study demonstrated that cementoblasts have the capacity to induce osteoclastogenesis, a capacity strongly promoted by IL-1β. These results may explain why osteoclasts can be formed next to the root of teeth. | nam c-n huynh vincent everts prasit pavasant ruchanee s ampornaramveth | 2017 | International Journal of Oral Science2017，9，4： | 5 |
| 2 | Preparation and characterization of microwave-treated carboxymethyl chitin and carboxymethyl chitosan films for potential use in wound care application显示文摘 | WONGPANIT PANYA SANCHAVANAKIT NEERACHA PAVASANT PRASIT | 2005 | Macromol Biosci2005，5，10： | 1 |
| 3 | Bone scaffolds from electrospun fiber mats of poly(3-hydroxybutyrate), poly(3-hydroxybutyrate- co -3-hydroxyvalerate) and their blend显示文摘 | Korakot Sombatmankhong Neeracha Sanchavanakit Prasit Pavasant Pitt Supaphol | 2007 | Polymer2007，，5： | 1 |
| 4 | Surface‐bound orientated Jagged‐1 enhances osteogenic differentiation of human periodontal ligament‐derived mesenchymal stem cells显示文摘 | Thanaphum Osathanon Patcharee Ritprajak Nunthawan Nowwarote Jeeranan Manokawinchoke Cecilia Giachelli Prasit Pavasant | 2012 | J. Biomed. Mater. Res2012，，2： | 1 |
| 5 | Characterization and biocompatibility of bacterial cellulose/alginate composite sponges with human keratinocytes and gingival fibroblasts显示文摘 | Nadda Chiaoprakobkij Neeracha Sanchavanakit Keskanya Subbalekha Prasit Pavasant Muenduen Phisalaphong | 2011 | Carbohydrate Polymers2011，，3： | 1 |
| 6 | Intermittent compressive force induces cell cycling and reduces apoptosis in embryoid bodies of mouse induced pluripotent stem cells显示文摘In vitro manipulation of induced pluripotent stem cells(iPSCs) by environmental factors is of great interest for three-dimensional(3D) tissue/organ induction. The effects of mechanical force depend on many factors, including force and cell type. However,information on such effects in i PSCs is lacking. The aim of this study was to identify a molecular mechanism in i PSCs responding to intermittent compressive force(ICF) by analyzing the global gene expression profile. Embryoid bodies of mouse i PSCs, attached on a tissue culture plate in 3D form, were subjected to ICF in serum-free culture medium for 24 h. Gene ontology analyses for RNA sequencing data demonstrated that genes differentially regulated by ICF were mainly associated with metabolic processes,membrane and protein binding. Topology-based analysis demonstrated that ICF induced genes in cell cycle categories and downregulated genes associated with metabolic processes. The Kyoto Encyclopedia of Genes and Genomes database revealed differentially regulated genes related to the p53 signaling pathway and cell cycle. q PCR analysis demonstrated significant upregulation of Ccnd1, Cdk6 and Ccng1. Flow cytometry showed that ICF induced cell cycle and proliferation, while reducing the number of apoptotic cells. ICF also upregulated transforming growth factor β1(Tgfb1) at both m RNA and protein levels, and pretreatment with a TGF-β inhibitor(SB431542) prior to ICF abolished ICF-induced Ccnd1 and Cdk6 expression. Taken together,these findings show that TGF-β signaling in i PSCs enhances proliferation and decreases apoptosis in response to ICF, that could give rise to an efficient protocol to manipulate i PSCs for organoid fabrication. | Jeeranan Manokawinchoke Phoonsuk Limraksasin Hiroko Okawa Prasit Pavasant Hiroshi Egusa Thanaphum Osathanon | 2022 | International Journal of Oral Science2022，14，1： | 1 |
| 7 | Tinospora crispa extract inhibits MMP-13 and migration of head and neck squamous cell carcinoma cell lines显示文摘Objective: To investigate the effect of Tinospora crispa(T. crispa) extract on matrix metalloproteinase 13(MMP-13) expression and cell migration. Methods: The cytotoxicity of T. crispa extract was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on head and neck squamous cell carcinoma(HNSCC) cell lines. The effect on expression of MMP-13 was analysed by RT-PCR and ELISA. The migration was assessed by wound healing assay. Results: MMP-13 m RNA was highly expressed in the metastatic human HNSCC cell lines, HN22 and HSC-3. T. crispa extract at a concentration of 100.0 μg/m L caused about 50% reduction of cell survival. T. crispa extract at a non-toxic concentration of 12.5, 25.0 and 50.0 μg/m L signii cantly suppressed MMP-13 m RNA expression and secreted MMP-13 in both HN22 and HSC-3. The expression of tissue inhibitors of metalloprotease by HSC-3 cells was attenuated by 25.0 and 50.0 μg/m L of T. crispa extract. Addition of the extract to cells in a wound healing assay showed inhibition of cell migration by HN22 cells. Conclusions: These data suggest that T. crispa could be considered as a potential therapeutic drug to prevent metastasis of HNSCC. | Hataipan Phienwej Ih-si Swasdichira Surattana Amnuoypol Prasit Pavasant Piyamas Sumrejkanchanakij | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，9： | 0 |
| 8 | Regulation of osteoprotegerin expression by Notch signaling in human oral squamous cell carcinoma cell line显示文摘Objective: To investigate the influence of Notch signaling on osteoprotegerin(OPG)expression in a human oral squamous cell carcinoma cell line.Methods: Activation of Notch signaling was performed by seeding cells on Jagged1 immobilized surfaces. In other experiments, a g-secretase inhibitor was added to the culture medium to inhibit intracellular Notch signaling. OPG m RNA and protein were determined by real-time PCR and ELISA, respectively. Finally, publicly available microarray database analysis was performed using connection up- or down-regulation expression analysis of microarrays software.Results: Jagged1-treatment of HSC-4 cells enhanced HES1 and HEY1 m RNA expression, confirming the intracellular activation of Notch signaling. OPG m RNA and protein levels were significantly suppressed upon Jagged1 treatment. Correspondingly, HSC-4 cells treated with a g-secretase inhibitor resulted in a significant reduction of HES1 and HEY1 m RNA levels, and a marked increase in OPG protein expression was observed.These results implied that Notch signaling regulated OPG expression in HSC-4 cells.However, Jagged1 did not alter OPG expression in another human oral squamous cell carcinoma cell line(HSC-5) or a human head and neck squamous cell carcinoma cell line(HN22).Conclusions: Notch signaling regulated OPG expression in an HSC-4 cell line and this mechanism could be cell line specific. | Jeeranan Manokawinchoke Thanaphum Osathanon Prasit Pavasant | 2016 | Asian Pacific Journal of Tropical Biomedicine2016，6，8： | 0 |
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