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| 1 | Decreased osteogenic activity and mineralization of alveolar bone cells from a patient with amelogenesis imperfecta and FAM83H 1261G>T mutation显示文摘FAM83H mutations lead to autosomal dominant hypocalcified amelogenesis imperfecta(ADHCAI).However,the biological role of FAM83H remains unclear.The present study aimed to characterize the alveolar bone cells isolated from a patient with ADHCAI having the mutation,c.1261G>T,p.E421*,in FAM83H.We showed that FAM83H mutant cells had proliferation ability and morphology similar to the controls.The F-actin staining revealed that FAM83H mutant cells were remained in the earlier stages of cell spreading compared to the controls at 30 min,but their spreading was advanced comparable to the controls at later stages.After osteogenic induction,a significant decrease in mRNA levels of RUNX2 and ALP was observed in FAM83H mutant cells at day 7 compared with day 3 while their expressions were increased in the controls.The OPN levels in FAM83H mutant cells were not significantly changed at day 7 compared to day 3 while the controls showed a significant increase.After 14 days,the mineral deposition of FAM83H mutant cells was slightly lower than that of the controls.In conclusion,we identify that FAM83H bone cells have lower expression of osteogenic marker genes and mineralization while they maintain their morphology,proliferation,and spreading.Consistent with previous studies in the ameloblasts and periodontal ligamental cells,these evidences propose that FAM83H influences osteogenic differentiation across different cell types in oral cavity. | Nunthawan Nowwarote Thanaphum Osathanon Kiattipan Kanjana Thanakorn Theerapanon Thantrira Porntaveetus Vorasuk Shotelersuk | 2019 | Genes & Diseases2019，6，4： | 2 |
| 2 | Transplantation of Cryopreserved Teeth:A Systematic Review显示文摘The aim of this article was to examine the research articles regarding biological and mechanical properties of cryopreserved teeth for potential use in tooth transplantation. A systematic review of literatures was performed by Pubmed searching with assigned key words from January 1, 1990 to June 8, 2009. All articles were examined for inclusion criteria. Secondary search was conducted by hand-search through references of included articles from primary search. A total of 24 articles were obtained from both primary and secondary search and used as fundamental articles in this review. Periodontal ligament tissues of cryopreserved teeth were able to maintain their biological properties resulted in a satisfactory healing of periodontium. Dental pulp tissues,however, may be compromised by limitation of permeability of cryopreservative agent into pulp cavity. Therefore, an endodontic treatment of transplanted cryopreserved teeth was recommended. Cryopreserved teeth had comparable mechanical properties to those of normal teeth. Importantly, the success of cryopreserved tooth transplantation treatment in orthodontic patients was reported. The cryopreserved teeth for tooth banking have a potential clinical application for treatment of missing teeth. Case selection, however, is critical for treatment success. More studies and data regarding masticatory function and periodontal healing of transplanted cryopreserved teeth are needed. | Thanaphum Osathanon | 2010 | International Journal of Oral Science2010，2，2： | 2 |
| 3 | Immobilization of alkaline phosphatase on microporous nanofibrous fibrin scaffolds for bone tissue engineering显示文摘 | OSATHANON T GIACHELLI C M SOMERMAN M J | 2009 | Biomaterials2009，30，27： | 1 |
| 4 | Ti-6A1-7Nb promotes eell spreading and fibroneetin and osteopontin synthesis in osteoblast-like eells显示文摘 | Osathanon T Bespinyowong K Arksornnukit M | 2006 | J Mater Sci Mater Med2006，17，7： | 1 |
| 5 | Notch signalling inhibits the adipogenic differentiation of single-cell-derived mesenchymal stem cell clones isolated from human adipose tissue显示文摘 | Osathanon T Subbalekha K Sastravaha P | 2012 | Cell biology international2012，36，12： | 1 |
| 6 | Basic fibroblast growthfactor inhibits mineralization but induces neuronal differentiationby human dental pulp stem cells through a FGFR and PLCγsig-naling pathway显示文摘 | Osathanon T Nowwarote N Pavasant P | 2011 | Cell Biochem2011，112，7： | 1 |
| 7 | Notch signal ing inhibits the adipogenic differentiation of single-cel-derived mesenchymal stem cellclones isolated from human adipose tissue显示文摘 | Osathanon T Subbalekha K Sastravaha P | | 0，，12： | 1 |
| 8 | Iloprost up-regulates vascular endo- thelial growth factor expression in human dental pulp ceils in vitro and enhances pulpal blood flow in vivo 显示文摘 | Limjeerajarus Chalida Nakalekha Osathanon Thanaphum Mano- kawinchoke Jeeranan | 2014 | Journal of endodon- tics2014，40，7： | 1 |
| 9 | Surface‐bound orientated Jagged‐1 enhances osteogenic differentiation of human periodontal ligament‐derived mesenchymal stem cells显示文摘 | Thanaphum Osathanon Patcharee Ritprajak Nunthawan Nowwarote Jeeranan Manokawinchoke Cecilia Giachelli Prasit Pavasant | 2012 | J. Biomed. Mater. Res2012，，2： | 1 |
| 10 | Magnetic bioassembly platforms towards the generation of extracellular vesicles from human salivary gland functional organoids for epithelial repair显示文摘Salivary glands(SG)are exocrine organs with secretory units commonly injured by radiotherapy.Bio-engineered organoids and extracellular vesicles(EV)are currently under investigation as potential strategies for SG repair.Herein,three-dimensional(3D)cultures of SG functional organoids(SGo)and human dental pulp stem cells(hDPSC)were generated by magnetic 3D bioassembly(M3DB)platforms.Fibroblast growth factor 10(FGF10)was used to enrich the SGo in secretory epithelial units.After 11 culture days via M3DB,SGo displayed SG-specific acinar epithelial units with functional properties upon neurostimulation.To consistently develop 3D hDPSC in vitro,3 culture days were sufficient to maintain hDPSC undifferentiated genotype and phenotype for EV generation.EV isolation was performed via sequential centrifugation of the conditioned media of hDPSC and SGo cultures.EV were characterized by nanoparticle tracking analysis,electron microscopy and immunoblotting.EV were in the exosome range for hDPSC(diameter:88.03±15.60 nm)and for SGo(123.15±63.06 nm).Upon ex vivo administration,exosomes derived from SGo significantly stimulated epithelial growth(up to 60%),mitosis,epithelial progenitors and neuronal growth in injured SG;however,such biological effects were less distinctive with the ones derived from hDPSC.Next,these exosome biological effects were investigated by proteomic arrays.Mass spectrometry profiling of SGo exosomes predicted that cellular growth,development and signaling was due to known and undocumented molecular targets downstream of FGF10.Semaphorins were identified as one of the novel targets requiring further investigations.Thus,M3DB platforms can generate exosomes with potential to ameliorate SG epithelial damage. | Ajjima Chansaenroj Christabella Adine Sawanya Charoenlappanit Sittiruk Roytrakul Ladawan Sariya Thanaphum Osathanon Sasitorn Rungarunlert Ganokon Urkasemsin Risa Chaisuparat Supansa Yodmuang Glauco R.Souza Joao N.Ferreira | 2022 | Bioactive Materials2022，7，12： | 1 |
| 11 | Microporous nanofibrous fibrin-based scaffolds for bone tissue engineering显示文摘 | Osathanon Thanaphum Linnes Michael L Rajachar Rupak M | 2008 | Biomaterials2008，29，30： | 1 |
| 12 | Ti- 6Al-7Nb promotes cell spreading and fibronectin and osteopontin synthesis in osteoblasts-like cells显示文摘 | Osathanon T Bespinyowong K Arksomnukit M | 2006 | J Mater Sci Mater Med2006，17，7： | 1 |
| 13 | Basic fibroblast growth factor inhibits mineralization but induces neuronal differentiation by human dental pulp stem cells through a FGFR and PLCgamma signaling pathway 显示文摘 | Osathanon T Nowwarote N Pavasant P | 2011 | J Cell Biochem2011，112，7： | 1 |
| 14 | b FGF and JAGGED1 regulate alkaline phosphatase expression and mineralization in dental tissue-derived mesenchymal stem cells显示文摘 | Osathanon T Nowwarote N Manokawinchoke J | 2013 | J Cell Biochem2013，114，11： | 1 |
| 15 | Basic fibroblast growth factor inhibits mineralization but induces neuronal differentiation by human dental pulp stem cells through a FGFR and PLC31 signaling pathway显示文摘 | Osathanon T Nowwarote N Pavasant P | 2011 | J Cell Biochem2011，112，7： | 1 |
| 16 | Basic fibroblastgrowth factor inhibits mineralization but induces neuronal dif-ferentiation by human dental pulp stem cells through a FGFRand PLC7 signaling pathway 显示文摘 | Osathanon T Nowwarote N Pavasant P | 2011 | J Cell Biochem2011，112，7： | 1 |
| 17 | Microporous nanofibrous fibrin-based scaffolds for bone tissue engineering显示文摘 | Osathanon T Linnes ML Rajachar RM | | 0，，30： | 1 |
| 18 | Intermittent compressive force induces cell cycling and reduces apoptosis in embryoid bodies of mouse induced pluripotent stem cells显示文摘In vitro manipulation of induced pluripotent stem cells(iPSCs) by environmental factors is of great interest for three-dimensional(3D) tissue/organ induction. The effects of mechanical force depend on many factors, including force and cell type. However,information on such effects in i PSCs is lacking. The aim of this study was to identify a molecular mechanism in i PSCs responding to intermittent compressive force(ICF) by analyzing the global gene expression profile. Embryoid bodies of mouse i PSCs, attached on a tissue culture plate in 3D form, were subjected to ICF in serum-free culture medium for 24 h. Gene ontology analyses for RNA sequencing data demonstrated that genes differentially regulated by ICF were mainly associated with metabolic processes,membrane and protein binding. Topology-based analysis demonstrated that ICF induced genes in cell cycle categories and downregulated genes associated with metabolic processes. The Kyoto Encyclopedia of Genes and Genomes database revealed differentially regulated genes related to the p53 signaling pathway and cell cycle. q PCR analysis demonstrated significant upregulation of Ccnd1, Cdk6 and Ccng1. Flow cytometry showed that ICF induced cell cycle and proliferation, while reducing the number of apoptotic cells. ICF also upregulated transforming growth factor β1(Tgfb1) at both m RNA and protein levels, and pretreatment with a TGF-β inhibitor(SB431542) prior to ICF abolished ICF-induced Ccnd1 and Cdk6 expression. Taken together,these findings show that TGF-β signaling in i PSCs enhances proliferation and decreases apoptosis in response to ICF, that could give rise to an efficient protocol to manipulate i PSCs for organoid fabrication. | Jeeranan Manokawinchoke Phoonsuk Limraksasin Hiroko Okawa Prasit Pavasant Hiroshi Egusa Thanaphum Osathanon | 2022 | International Journal of Oral Science2022，14，1： | 1 |
| 19 | Regulation of osteoprotegerin expression by Notch signaling in human oral squamous cell carcinoma cell line显示文摘Objective: To investigate the influence of Notch signaling on osteoprotegerin(OPG)expression in a human oral squamous cell carcinoma cell line.Methods: Activation of Notch signaling was performed by seeding cells on Jagged1 immobilized surfaces. In other experiments, a g-secretase inhibitor was added to the culture medium to inhibit intracellular Notch signaling. OPG m RNA and protein were determined by real-time PCR and ELISA, respectively. Finally, publicly available microarray database analysis was performed using connection up- or down-regulation expression analysis of microarrays software.Results: Jagged1-treatment of HSC-4 cells enhanced HES1 and HEY1 m RNA expression, confirming the intracellular activation of Notch signaling. OPG m RNA and protein levels were significantly suppressed upon Jagged1 treatment. Correspondingly, HSC-4 cells treated with a g-secretase inhibitor resulted in a significant reduction of HES1 and HEY1 m RNA levels, and a marked increase in OPG protein expression was observed.These results implied that Notch signaling regulated OPG expression in HSC-4 cells.However, Jagged1 did not alter OPG expression in another human oral squamous cell carcinoma cell line(HSC-5) or a human head and neck squamous cell carcinoma cell line(HN22).Conclusions: Notch signaling regulated OPG expression in an HSC-4 cell line and this mechanism could be cell line specific. | Jeeranan Manokawinchoke Thanaphum Osathanon Prasit Pavasant | 2016 | Asian Pacific Journal of Tropical Biomedicine2016，6，8： | 0 |
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