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| 1 | 一种miRNA标记在胶质瘤侵袭性表型的确定与预后评估中的价值(英文)显示文摘Gliomas represent a disparate group of tumours for which there are to date no cure. Thus,there is a recognized need for new diagnostic and therapeutic approaches based on increased understanding of their molecular nature. We performed the comparison of the microRNA( miRNA) profile of 8 WHO grade II gliomas and 24 higher grade tumours( 2 WHO grade III and 22 glioblastomas) by using the Affymetrix Gene Chip miRNA Array v. 1. 0. A relative quantification method( RT-q PCR) with standard curve was used to confirm the 22 miRNA signature resulted by array analysis. The prognostic performances of the confirmed miRNAs were estimated on the Tumor Cancer Genome Atlas( TCGA) datasets. We identified 22 miRNAs distinguishing grade II gliomas from higher grade tumours.RT-q PCR confirmed the differential expression in the two patients' groups for 13 out of the 22 miRNAs. The analysis of the Glioblastoma Multiforme( GBM) and Lower Grade Glioma( LGG) datasets from TCGA demonstrated the association with prognosis for 6 of those miRNAs. Moreover,in the GBM dataset miR-21 and miR-210 were predictors of worse prognosis in both univariable and multivariable Cox regression analyses( HR 1. 19,P = 0. 04,and HR 1. 18,P = 0. 029 respectively). Our results support a direct contribution of miRNAs to glioma cancerogenesis and suggest that miR-21 and miR-210 may play a role in the aggressive clinical behaviour of glioblastomas. | Barbano R Palumbo O Pasculli B Galasso M Volinia S D'Angelo V Icolaro N Coco M Dimitri L Graziano P Copetti M Valori VM Maiello E Carella M Fazio VM Parrella P | 2014 | 中华神经外科疾病研究杂志2014，13，5： | 6 |
| 2 | DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor显示文摘AIM: To determine the DNA binding activity and protein levels of the Ku70/80 heterodimer, the functional mediator of the NHEJ activity, in human colorectal carcinogenesis.METHODS: The Ku70/80 DNA-binding activity was determined by electrophoretic mobility shift assays in 20 colon adenoma and 15 colorectal cancer samples as well as matched normal colonic tissues. Nuclear and cytoplasmic protein expression was determined by immunohistochemistry and Western blot analysis.RESULTS: A statistically significant difference was found in both adenomas and carcinomas as compared to matched normal colonic mucosa (P<0.00). However,changes in binding activity were not homogenous with approximately 50% of the tumors showing a clear increase in the binding activity, 30% displaying a modest increase and 15% showing a decrease of the activity.Tumors, with increased DNA-binding activity, also showed a statistically significant increase in Ku70 and Ku86nuclear expression, as determined by Western blot and immunohistochemical analyses (P<0.001). Cytoplasmic protein expression was found in pathological samples,but not in normal tissues either from tumor patients or from healthy subjects.CONCLUSION: Overall, our DNA-binding activity and protein level are consistent with a substantial activation of the NHEJ pathway in colorectal tumors. Since the NHEJ is an error prone mechanism, its abnormal activation can result in chromosomal instability and ultimately lead to tumorigenesis. | Paola Mazzarelli Paola Parrella Davide Seripa Emanuela Signori Giuseppe Perrone Carla Rabitti Domenico Borzomati Armando Gabbrielli Maria Giovanna Matera Carolina Gravina Marco Caricato Maria Luana Poeta Monica Rinaldi Sergio Valeri Roberto Coppola Vito Michele Fazio | 2005 | World Journal of Gastroenterology2005，11，42： | 4 |
| 3 | Promotion of vascular smooth muscle cell growth by homocysteine显示文摘 | Tsai JC Parrella MA Yoshizumi M | | 0，，： | 1 |
| 4 | P38MAPK-de- pendent phosphorylation and degradation of SRC-3/AIB1 and RARalpha-mediated transcription显示文摘 | Gianni M Parrella E Raska I Jr | 2006 | EMBO J2006，25，4： | 1 |
| 5 | An update of the host range of Tomato spotted wilt virus显示文摘 | Parrella G Gognalons P Gebre- Selassie K | 2003 | Journal of Plant Pathology2003，85，4： | 1 |
| 6 | Biological studies of Liriomyza huidobrensis (Diptera:Agromyzidae) on chrysanthemum,aster,and pea显示文摘 | PARRELLA M P BETHKE J A | 1984 | J Econ Entomol1984，77，： | 1 |
| 7 | Detection of mitochondrial DNA mutations in primary breast cancer and fine-needle aspiratesl显示文摘 | Parrella P Xiao Y Fliss M | 2001 | Cancer Res2001，61，20： | 1 |
| 8 | Intraoperative infusion of amino acids induces anabolism independent of the type of anesthesia显示文摘 | Donatelli F Schricker T Parrella P | 2006 | Anesth Analg2006，103，： | 1 |
| 9 | Enhanced expression of glucose - 6 - phosphate dehydrogenase in human cells sustaining oxidative stress 显示文摘 | Ursini MV Parrella A Rosa G | 1997 | Biochem J1997，323，3： | 1 |
| 10 | Predatory mites help control thrips on floriculture crops显示文摘 | Hessein N A Parrella M P | 1990 | California Agriculture1990，44，6： | 1 |
| 11 | 显示文摘 | Brennan C J Parrella R D Larsen D E | 1994 | Ferrloelectrics1994，151，： | 1 |
| 12 | Biology of Liriomyza显示文摘 | Parrella M P | 1997 | Ann Rev Entomo11997，，32： | 1 |
| 13 | Identification of a mononucleotide repeat as a major target for mitochondrial DNA alterations in human tumors显示文摘 | Sanchez-Cespedes M Parrella P Nomoto S | 2001 | Cancer Res2001，61，19： | 1 |
| 14 | Detection of mitochondrial DNA mutations in primary, breast cancer and fine-needle aspirates显示文摘 | Parrella P Yan X Fliss M | 2001 | Cancer Res2001，61，20： | 1 |
| 15 | The Sublethal Effects of Selected Insecticides on Life Table Parameters of Panonychus cirri ( Acari: Tetranychidae) 显示文摘 | JONES V P PARRELLA M P | 1984 | Can Entomol1984，116，7： | 1 |
| 16 | Inactivation of LKB1/STK11 is a common event in adenocarcinomas of the lung显示文摘 | Sanchez-Cespedes M Parrella P Esteller M | 2002 | Cancer Res2002，62，13： | 1 |
| 17 | Inactivation of LKB1/STK11 is a common event in adenocarcinomas of the lung显示文摘 | Sanchez-Cespedes M Parrella P Esteller M | 2002 | Cancer Res2002，62，13： | 1 |
| 18 | First report of Tomato chlorosis virus (ToCV) in Campania and of Tomato infectious chlorosis virus (TICV) in Calabria (Southern Italy)显示文摘 | Parrella G Scassillo L | 2006 | Informatore Fitopato logico2006，56，6： | 1 |
| 19 | Predatory mites help control thrips on floriculture crops 显示文摘 | Hessein NA Parrella MP | 1990 | California Agriculture1990，44，6： | 1 |
| 20 | Detection of c-myc amplification in uveal melanoma by fluorescent in situ hybridization 显示文摘 | Parrella P Caballero OL Sidransky D | 2001 | Invest Ophthalmol Vis Sci2001，42，8： | 1 |
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