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| 1 | Differential gene expression of chemokines in KRAS and BRAF mutated colorectal cell lines:Role of cytokines显示文摘AIM:To study KRAS/BRAF mutations in colorectalcancer(CRC)that influences the efficacy of treatment.To develop strategies for overcoming combination of treatment.METHODS:Five colonic cell-lines were investigated:DLD-1 with KRAS(G13D)mutation,HT 29 and Colo205 with BRAF(V600E)mutation as well as the wild type(Wt)cell-lines Caco2 and Colo-320.DLD-1(KRAS),HT-29(BRAF)and Caco2(Wt)cell lines were treated with cytokines(TNFα50 ng,IL-1β1 ng and IFNγ50ng)and harvested at different time points(1-24 h).KRAS inhibition was performed by the siRNA-approach.Two colorectal cancer cells DLD-1 and Caco2 were used for KRAS inhibition.About 70%confluency were confirmed before transfection with small interferring RNA(siRNA)oligonucleotides.All the synthetic siRNA sequences were designed in our laboratory.Total RNA and protein was isolated from the cells for RT-PCR and Western blotting.Densitometry of the Western blotting was analyzed with the Image J software(NIH).Results are shown as mean±SD.RESULTS:RT-PCR analysis in non-stimulated cells showed a low basal expression of TNFαand IL-1βin the DLD-1 KRAS-mutated cell-line,compared to Caco2wild type.No detection was found for IL-6 and IFNγin any of the studied cell lines.In contrast,pro-angiogenic chemokines(CXCL1,CXCL8)showed a high constitutive expression in the mutated cell-lines DLD-1(KRAS),HT-29 and Colo205(BRAF),compared to wild type(Caco2).The anti-angiogenic chemokine(CXCL10)showed a high basal expression in wild-type,compared to mutated cell-lines.KRAS down-regulation by siRNA showed a significant decrease in CXCL1 and CXCL10gene expression in the DLD-1(KRAS)cell-line in comparison to wild type(Caco2)at 72 h after KRAS silencing.In contrast,the specific KRAS inhibition resulted in an up-regulation of CXCL1 and CXCL10.The results of our study show a higher expression of pro-angiogenic chemokines at basal level in mutated cell-lines,which was further increased by cytokine treatment.CONCLUSION:To summarize,basal chemokine gene expression for pro-angiogenic chemokines was high in mutated as compared to wild type cell-lines.This reflects the likely existence of a different microenvironment in tumours consistent of wild type or mutated cells.This may help to rationalize the choice of molecular targets for suitable therapeutic investigation in clinical studies. | Sajjad Khan Silke Cameron Martina Blaschke Federico Moriconi Naila Naz Ahmad Amanzada Giuliano Ramadori Ihtzaz Ahmed Malik | 2014 | World Journal of Gastroenterology2014，20，11： | 6 |
| 2 | Single-Dose Gamma-Irradiation Induces Up-Regulation of Chemokine Gene Expression and Recruitment of Granulocytes into the Portal Area but Not into Other Regions of Rat Hepatic Tissue显示文摘 | Ihtzaz Ahmed Malik Federico Moriconi Nadeem Sheikh Naila Naz Sajjad Khan Jozsef Dudas Tümen Mansuroglu Clemens Friedrich Hess Margret Rave-Fr?nk Hans Christiansen Giuliano Ramadori | 2010 | The American Journal of Pathology2010，，4： | 2 |
| 3 | Lamivudine-resistant HBV strain rtM204V/I in acute hepatitis B显示文摘 | Nicola Coppola Gilda Tonziello Piero Colombatto Mariantonietta Pisaturo Vincenzo Messina Francesco Moriconi Loredana Alessio Caterina Sagnelli Daniela Cavallone Maurizia Brunetto Evangelista Sagnelli | 2013 | Journal of Infection2013，，： | 2 |
| 4 | Nano-precision metrology of X-ray mirrors with laser speckle angular measurement显示文摘X-ray mirrors are widely used for synchrotron radiation,free-electron lasers,and astronomical telescopes.The short wavelength and grazing incidence impose strict limits on the permissible slope error.Advanced polishing techniques have already produced mirrors with slope errors below 50 nrad root mean square(rms),but existing metrology techniques struggle to measure them.Here,we describe a laser speckle angular measurement(SAM)approach to overcome such limitations.We also demonstrate that the angular precision of slope error measurements can be pushed down to 20nrad rms by utilizing an advanced sub-pixel tracking algorithm.Furthermore,SAM allows the measurement of mirrors in two dimensions with radii of curvature as low as a few hundred millimeters.Importantly,the instrument based on SAM is compact,low-cost,and easy to integrate with most other existing X-ray mirror metrology instruments,such as the long trace profiler(LTP)and nanometer optical metrology(NOM).The proposed nanometrology method represents an important milestone and potentially opens up new possibilities to develop next-generation super-polished X-ray mirrors,which will advance the development of X-ray nanoprobes,coherence preservation,and astronomical physics. | Hongchang Wang Simone Moriconi Kawal Sawhney | 2021 | Light(Science & Applications)2021，10，10： | 2 |
| 5 | Correct architecture refinement显示文摘 | MORICONI M QIAN XL RIEMENSCHNEIDER RA | 1995 | IEEE Transactions on Software Engineering1995，21，4： | 1 |
| 6 | Influence of lignosulphonate, glucose and gulconate on the C3A hydration 显示文摘 | MONOSI S MORICONI G PAURI M | 1983 | Cem Concr Res1983，13，4： | 1 |
| 7 | Hepatitis B surface antigen serum levels help to distinguish active from inactive hepatitis B virus genotype D carriers 显示文摘 | Brunetto MR Oliveri F Colombatto P Moriconi F Ciccorossi P Coco B Romagnoli V Cherubini B Moscato G Maina AM Cavallone D Bonino F | 2010 | Gastroenterology2010，139，2： | 1 |
| 8 | Influ ence of gluconate, lignosulfonate or glucose on the C3A hydration in the presence of gypsum with or without lime显示文摘 | COLLEPARDI M MONOSI S MORICONI G | 1984 | Cem Concr Res1984，14，1： | 1 |
| 9 | Proteinuria in focal segmental glomerulosclerosis:role of circulating factors and therapeutic approach显示文摘 | Moriconi L Lenti C Puccini R | 2001 | Ran Fail2001，23，： | 1 |
| 10 | Environmentally-friendly mortars: a way to improve bond between mortar and brick显示文摘 | G. Moriconi V. Corinaldesi R. Antonucci | 2003 | Materials and Structures2003，，10： | 1 |
| 11 | Hepatitis B virus surface antigen levels: a guide to sustained response to peginterferon alfa-2a in HBeAg-negative chronic hepatitis B 显示文摘 | Brunetto MR Moriconi F Bonino F | 2009 | Hepatology2009，49，4： | 1 |
| 12 | Hepatitis B virus surface antigen levels:a guide to sustained response to pegin-terferon alfa-2a in HBeAg-negative chronic hepatitis B显示文摘 | Brunetto MR Moriconi F Bonino F | | 0，，04： | 1 |
| 13 | TAZ is required for metastatic activity and chemoresistance of breast cancer stem cells 显示文摘 | Bartucci M Dattilo R Moriconi C | 2015 | Oncogene2015，34，6： | 1 |
| 14 | Behavior of beam-column jionts made of sustainable concrete under cyclic loading显示文摘 | Corinaldesi V Moriconi G | 2006 | Journal of Materi- als in Civil Engineering2006，，5： | 1 |
| 15 | Use of Rubble from Building Demolition in Mortars显示文摘 | Corinaldesi V Giuggiolini M Moriconi G | 2002 | Waste Manage2002，22，8： | 1 |
| 16 | Hepatitis B virus surface antigen levels: a guide to sustained response to peginterferon alfa-2a in HBeAg-negative chronic hepatitis B 显示文摘 | Brunetto MR Moriconi F Bonino F Lau GK Farci 19 Yurdaydin C Piratvisuth T Luo K Wang Y Hadziyannis S Wolf E McCloud P Batrla R Marcellin P | 2009 | Hepatology2009，49，4： | 1 |
| 17 | Body composition and adiponectin serum concentrations in adult patients with cystic fi brosis显示文摘 | Moriconi N Kraenzlin M Puder JJ | | 0，，04： | 1 |
| 18 | Multicenter evaluation of the elecsys hepatitis B surface antigen quantitative assay显示文摘 | Zacher B J Moriconi F Bowden S | 2011 | Clin Vaccine Immunol2011，18，1： | 1 |
| 19 | Durable fiber reinforced self- compacting concrete 显示文摘 | CORINALDESI V MORICONI G | 2004 | Cement and Concrete Research2004，，34： | 1 |
| 20 | Characterization of an aluminum-filled polyamide powder for applications in selective laser sintering显示文摘 | MAZZOLI A MORICONI G PAURI G M | 2007 | Materials and Design2007，28，3： | 1 |
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