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| 1 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 2 | Vitamin C Enhances the Generation of Mouse and Human Induced Pluripotent Stem Cells显示文摘 | Miguel Angel Esteban Tao Wang Baoming Qin Jiayin Yang Dajiang Qin Jinglei Cai Wen Li Zhihui Weng Jiekai Chen Su Ni Keshi Chen Yuan Li Xiaopeng Liu Jianyong Xu Shiqiang Zhang Feng Li Wenzhi He Krystyna Labuda Yancheng Song Anja Peterbauer Susanne Wolbank H | 2010 | Cell Stem Cell2010，，： | 3 |
| 3 | Effect of closure of live poultry markets on poultry-to-person transmission of avian influenza A H7N9 virus: an ecological study显示文摘 | Hongjie Yu Joseph T Wu Benjamin J Cowling Qiaohong Liao Vicky J Fang Sheng Zhou Peng Wu Hang Zhou Eric H Y Lau Danhuai Guo Michael Y Ni Zhibin Peng Luzhao Feng Hui Jiang Huiming Luo Qun Li Zijian Feng Yu Wang Weizhong Yang Gabriel M Leung | 2013 | The Lancet2013，，： | 2 |
| 4 | Chemically functionalized carbon nanotubes as substrates for neuronal growth显示文摘 | Hu H Ni Y Montana V | 2004 | Nano Lett2004，4，： | 1 |
| 5 | 显示文摘 | Ni H L Zhang J S | 2001 | Polym Mat Sci Engin2001，17，5： | 1 |
| 6 | 7-O-descarbamoyl-7- hydroxygeldanamycin, a minor component from gdmN disruption mutant of Streptomyces hygroscopicus 17997显示文摘 | Ni S Y Wu L Z Wang H Y | 2010 | JAntibiot(Tokyo)2010，63，10： | 1 |
| 7 | Origin and evolution of Japanese encephalitis virus in southeast Asia 显示文摘 | Solomon T Ni H Beasley D W | 2003 | J Virol2003，77，5： | 1 |
| 8 | Prediction methodol- ogy for ground vibration induced by passing trains on bridge structures 显示文摘 | Chen Y J Ju S H Ni S H | 2007 | Journal of Sound and Vibration2007，302，45： | 1 |
| 9 | C-reactive protein as a predictor of mor- tality in critically ill patients:a meta-analysis and system- atic review显示文摘 | Zhang Z Ni H | 2011 | Anaesth Intensive Care2011，39，5： | 1 |
| 10 | Acinar cell carcinoma of the pancreas显示文摘 | Hartman GG Ni H Pickleman J | 2001 | Arch Pathol Lab Med2001，125，8： | 1 |
| 11 | Atomistic Computer Simulation of Fracture Process At Nanoscale显示文摘 | Wu H A Ni X G Wang X X | 2004 | Key Engineering Materials2004，，： | 1 |
| 12 | Cationic montmorillonite: Preparation and synergy with anionic polymer in filler flocculation显示文摘 | Liu W Ni Y Xiao H | 2003 | JOURNAL OF PULP AND PAPER SCIENCE2003，29，5： | 1 |
| 13 | A riccati equation approach to the design of linear robust controllers显示文摘 | Ni M L Wei H X | 1995 | Automatica1995，29，6： | 1 |
| 14 | An overview of hydrogen production from biomass显示文摘 | Meng Ni Dennis Y C Leung Michael K H Leung Sumathy K | 2006 | Fuel Processing Technology2006，，87： | 1 |
| 15 | TIP30/CC3 expression in breast carcinoma: relation to metastasis 显示文摘 | ZHAO J NI H MA Y | 2007 | Clinic Pathol2007，38，2： | 1 |
| 16 | Binding of phytochrome B to its nuclear signalling partner PIF3 is reversibly induced by light显示文摘 | Ni M Tepperman J M Quail P H | 1999 | Nature1999，400，： | 1 |
| 17 | More than 10 years' continuous oral treatment with specific immunoglobulin Y for the prevention of Pseudomonas aeruginosa infections : a case report显示文摘 | Ni|sson E Kollberg H Johannesson M | 2007 | J MedFood2007，10，2： | 1 |
| 18 | The analysis of inorganic and organic pollu- tants in soil with special regard to their bioavailability 显示文摘 | H ni H | 1990 | International journal of environmental analytical1990，39，： | 1 |
| 19 | Temperature dependence of frictional force in carbon nanotube oscillators显示文摘 | Chen Y F Yang J K Wang X H Ni Z H Li D Y | 2009 | Nanotechnology2009，20，03： | 1 |
| 20 | Organosoluble Semi-alicyclic Polyimides Derived from 3,4-dicarboxy-l,2,3, 4 -tetrahydro-6-tert-butyl-l-naphthalene Succinic Dianhy- dride and Aromatic Diamines: Synthesis, Characterization and Thermal Degradation Investigation显示文摘 | Guo Y Z Shen D 'X Ni H J | 2013 | Progress in Or- ganic Coatings2013，76，4： | 1 |
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