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| 1 | Inhibition of the B7-H3 immune checkpoint limits tumor growth by enhancing cytotoxic lymphocyte function显示文摘在肿瘤和免疫系统之间的相互作用仍然糟糕被理解。重要临床的回答在对 CTLA4 和 PD-1/PD-L1 检查点与抗体对待的癌症病人被完成了;然而,仅仅病人的小部分对治疗作出回应,显示需要探索为癌症治疗的另外的 co 禁止的分子。B7-H3, B7 总科的一个成员,被我们以前显示出禁止 T 房间激活和 autoimmunity。在这研究,我们在肿瘤免疫分析了 B7-H3 的功能。B7-H3 的表示在多重肿瘤线,渗入肿瘤的树枝状的房间,和巨噬细胞被发现。与对抗抗体对待到 B7-H3 的 B7-H3-deficient 老鼠或老鼠显示出多重肿瘤的减少的生长,它取决于 NK 和 CD8 + T 房间。与细胞毒素的淋巴细胞表示的通常认为的受体, B7-H3 禁止了他们的激活,并且它的缺乏在忍受肿瘤的鼠标导致了增加的细胞毒素的淋巴细胞功能。B7-H3 和 PD-1 的联合封锁导致了迟了阶段的肿瘤的进一步提高的治疗学的控制。一起拿,我们的结果显示 B7-H3 检查点可以对癌症为免疫疗法用作一个新奇目标。 | Young-hee Lee Natalia Martin-Orozco Peilin Zheng Jing Li Peng Zhang Haidong Tan Hyun Jung Park Mira Jeong Seon Hee Chang Byung-Seok Kim Wei Xiong Wenjuan Zang Li Guo Yang Liu Zhong-jun Dong Willem W Overwijk Patrick Hwu Qing Yi Larry Kwak Zhiying Yang Tak W Mak Wei-Li Laszlo G Radvanyi Ling Ni Dongfang Liu Chen Dong | 2017 | Cell Research2017，27，8： | 29 |
| 2 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 3 | An antagonist decoy receptor and a death domain-containing receptor for TRAIL显示文摘 | Pan G Ni G Wei YF Ni J Yu G | | 0，，： | 3 |
| 4 | Geometric phase in spin precession and the adiabatic approximation 显示文摘 | Ni G J Chen S Q Shen Y L | 1995 | Phys Lett1995，197，： | 2 |
| 5 | An antagonist decoy receptor and a death domain-containing receptor for TRAIL 显示文摘 | Pan G Ni J Wei Y F | 1997 | Science1997，277，5327： | 2 |
| 6 | Random seismic response analysis of adjacent buildings coupled with non-linear hysteretic dampers显示文摘 | Ni Y Q Ko J M Ying Z G | 2001 | Journal of Sound and Vibration2001，246，3： | 1 |
| 7 | Atomistic Computer Simulation of Fracture Process At Nanoscale显示文摘 | Wu H A Ni X G Wang X X | 2004 | Key Engineering Materials2004，，： | 1 |
| 8 | Age-related de- clines in car following performance under simulated fog conditions显示文摘 | Ni R Kang J J Andersen G J | 2010 | Accident Analysis and Preven- tion2010，42，3： | 1 |
| 9 | Effect of cat- alysts on the reaction of an aliphatic isocyanate and wa- ter 显示文摘 | NI H NASH H A WORDEN J G | 2002 | J Appl Polym Sci Part A: Polym Chem2002，0，11： | 1 |
| 10 | Statistical inter- pretation of the daily variation of urban water consumption in Beijing, China显示文摘 | ZHANG D W NI G H CONG Z T | 2012 | Hydrological Sciences Journal2012，59，1： | 1 |
| 11 | A Particle Swarm Optimization Method with Enhanced Global Search Ability for Design Optimizations of Electromagnetic Devices显示文摘 | Ho S L Yang S Y Ni G Z | 2006 | IEEE Trans on Magnetics2006，42，4： | 1 |
| 12 | An antagonist decoy receptor and a death domain-containing receptor for TRAIL 显示文摘 | Pan G Ni J Wei YF | 1997 | Science1997，277，5237： | 1 |
| 13 | Activation of beta2-adrenergic receptor stimulates gamma - secretase activity and accelerates amyloid plaque formation显示文摘 | Ni Y Zhao X Bao G | 2006 | Nat Med2006，12，12： | 1 |
| 14 | Polyhedral oligomeric silsesquioxane (POSS) polymers and copolymers: a review 显示文摘 | Li G Z Wang L C Ni H L | 2001 | Journal of Inorganic and Organometallic Polymers2001，11，3： | 1 |
| 15 | Stomatal and nonstomatal limitations to net photosynthesis in seedlings of woody angiosperms显示文摘 | Ni B R Pallardy S G | 1992 | Plant Physical1992，99，15： | 1 |
| 16 | Occurrence of chlorinated and brominated polycyclic aromatic hydrocarbons in surface sediments in Shenzhen, South China and its relationship to urbanization显示文摘 | Sun J L Ni H G Zeng H | 2011 | Journal of Environmental Monitoring2011，13，10： | 1 |
| 17 | 查看详情显示文摘 | Wen H H Li S L Zhao Z W Ni Y M Ren Z A Che G C Zhao Z X | | 0，，： | 1 |
| 18 | Modeling of the size effects on the behavior of metals in mieroscale deformation proeesses显示文摘 | Kim G Y Ni J Koc M | 2007 | Journal of Manufacturing Science and Engineering Transactions of the ASME2007，129，3： | 1 |
| 19 | An Evolutionary Algorithm for Fractal Coding of Binary Images显示文摘 | Dasgupta D Hernandez G Ni(n)o F | | 0，，02： | 1 |
| 20 | The preparation of nano-sized H ZSM-5 zeolite and its application in the aromatization of methanol 显示文摘 | NI Y M SUN A M WU X L HAl G L HU J L LI T LI G X | 2011 | Microporous and Mesoporous Materials2011，143，2： | 1 |
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