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| 1 | Seroprevalence of Babesia caballi and Theileria equi in five draught equine populated metropolises of Punjab, Pakistan显示文摘 | Muhammad Hammad Hussain Muhammad Saqib Fahad Raza Ghulam Muhammad Muhammad Nadeem Asi Muhammad Khalid Mansoor Muhammad Saleem Abdul Jabbar | 2014 | Veterinary Parasitology2014，，： | 1 |
| 2 | Smart Dynamic Trafc Monitoring and Enforcement Syste显示文摘Enforcement of trafc rules and regulations involves a wide range of complex tasks,many of which demand the use of modern technologies.variable speed limits(VSL)control is to change the current speed limit according to the current trafc situation based on the observed trafc conditions.The aim of this study is to provide a simulation-based methodological framework to evaluate(VSL)as an effective Intelligent Transportation System(ITS)enforcement system.The focus of the study is on measuring the effectiveness of the dynamic trafc control strategy on trafc performance and safety considering various performance indicators such as total travel time,average delay,and average number of stops.United Arab Emirates(UAE)was selected as a case study to evaluate the effectiveness of this strategy.A micro simulation software package VISSIM with add-on module VisVAP is used to evaluate the impacts of VSL.It has been observed that VSL control strategy reduced the average delay time per vehicle to around 7%,travel time by 3.2%,and number of stops by 48.5%.Dynamic trafc control strategies also alleviated congestion by increasing the capacity of the bottleneck section and improving safety.Results of this study would act as a guidance for engineers and decision makers to new trafc control system implementation. | Youssef El-Hansali Fatma Outay Ansar Yasar Siham Farrag Muhammad Shoaib Muhammad Imran Hammad Hussain Awan | 2021 | Computers, Materials & Continua2021，，6： | 0 |
| 3 | Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry显示文摘Microbial cell factories(bacteria and fungi)are the leading producers of beneficial natural products such as lycopene,carotene,herbal medicine,and biodiesel etc.These microorganisms are considered efficient due to their effective bioprocessing strategy(monoculture-and consortial-based approach)under distinct processing conditions.Meanwhile,the advancement in genetic and process optimization techniques leads to enhanced biosynthesis of natural products that are known functional ingredients with numerous applications in the food,cosmetic and medical industries.Natural consortia and monoculture thrive in nature in a small proportion,such as wastewater,food products,and soils.In similitude to natural consortia,it is possible to engineer artificial microbial consortia and program their behaviours via synthetic biology tools.Therefore,this review summarizes the optimization of genetic and physicochemical parameters of the microbial system for improved production of natural products.Also,this review presents a brief history of natural consortium and describes the functional properties of monocultures.This review focuses on synthetic biology tools that enable new approaches to design synthetic consortia;and highlights the syntropic interactions that determine the performance and stability of synthetic consortia.In particular,the effect of processing conditions and advanced genetic techniques to improve the productibility of both monoculture and consortial based systems have been greatly emphasized.In this context,possible strategies are also discussed to give an insight into microbial engineering for improved pro-duction of natural products in the future.In summary,it is concluded that the coupling of genomic modifications with optimum physicochemical factors would be promising for producing a robust microbial cell factory that shall contribute to the increased production of natural products. | Muhammad Hammad Hussain Muhammad Zubair Mohsin Waqas Qamar Zaman Junxiong Yu Xueli Zhao Yanlong Wei Yingping Zhuang Ali Mohsin Meijin Guo | 2022 | Synthetic and Systems Biotechnology2022，7，1： | 0 |
| 4 | Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate(MVA)and lycopene expression pathways显示文摘The stability and high productivity of heterogeneous terpenoid production in Escherichia coli expression system is one of the most key issues for its large scale industrialization.In the current study on taking lycopene biosynthesis as an example,an integrated Escherichia coli system has been generated successfully,which resulted into stable and high lycopene production.In this process,two modules of mevalonate(MVA)pathway and one module of lycopene expression pathway were completely integrated in the chromosome.Firstly,the copy number and integrated position of three modules of heterologous pathways were rationally optimized.Later,a strain DH416 equipped with heterogeneous expression pathways through chromosomal integration was efficiently derived from parental strain DH411.The evolving DH416 strain efficiently produced the lycopene level of 1.22 g/L(49.9 mg/g DCW)in a 5 L fermenter with mean productivity of 61.0 mg/L/h.Additionally,the integrated strain showed more genetic stability than the plasmid systems after successive 21st passage. | Muhammad Hammad Hussain Qi Hong Waqas Qamar Zaman Ali Mohsin Yanlong Wei Ning Zhang Hongqing Fang Zejian Wang Haifeng Hang Yingping Zhuang Meijin Guo | 2021 | Synthetic and Systems Biotechnology2021，6，2： | 0 |
| 5 | A decline in snow cover area of Passu and Ghulkin valleys between 1995 and 2022显示文摘The global cryosphere is experiencing accelerated melting due to climate change.Currently,the Karakoram anomaly is under discussion with a debate about the possibility that the anomaly may have recently ended.This study aims to evaluate the up-to-date changes in snow cover in the western Karakoram region.We observed the snow cover changes in Passu and Ghulkin valleys in the Hunza River basin(HRB)of the Karakoram through multitemporal Landsat satellite data between 1995 and 2022.We found a significant reduction in snow cover in these valleys,with an average reduction rate of 0.42 km~2/yr,resulting in a total reduction of~11.46 km~2 between 1995 and 2022.This reduction in snow cover is consistent with the mass loss of glaciers in the Karakoram region in recent years.The decline in snow cover in these valleys is also consistent with the meteorological data.The temperature in summer(June)has significantly increased whereas the precipitation in the accumulation season(March)has decreased.These rapid changes suggest that it is crucially important to monitor the snow cover on a regular basis to support downstream management of snowmelt runoff.In addition,there is a need of planning for mitigation and adaptation strategies for snow-related hazards. | Imran HUSSAIN Mumtaz ALI Sher MUHAMMAD Wajid HASSAN Salar ALI Zakir HUSSAIN Syed Hammad ALI Shaukat ALI Muhammad Younis KHAN | 2023 | Journal of Mountain Science2023，20，12： | 0 |
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