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| 1 | The Scorpion Venom Peptide Smp76 Inhibits Viral Infection by Regulating Type-Ⅰ Interferon Response显示文摘Dengue virus(DENV) and Zika virus(ZIKV) have spread throughout many countries in the developing world and infect millions of people every year, causing severe harm to human health and the economy. Unfortunately, there are few effective vaccines and therapies available against these viruses. Therefore, the discovery of new antiviral agents is critical.Herein, a scorpion venom peptide(Smp76) characterized from Scorpio maurus palmatus was successfully expressed and purified in Escherichia coli BL21(DE3). The recombinant Smp76(rSmp76) was found to effectively inhibit DENV and ZIKV infections in a dose-dependent manner in both cultured cell lines and primary mouse macrophages. Interestingly,rSmp76 did not inactivate the viral particles directly but suppressed the established viral infection, similar to the effect of interferon(IFN)-b. Mechanistically, rSmp76 was revealed to upregulate the expression of IFN-b by activating interferon regulatory transcription factor 3(IRF3) phosphorylation, enhancing the type-Ⅰ IFN response and inhibiting viral infection.This mechanism is significantly different from traditional virucidal antimicrobial peptides(AMPs). Overall, the scorpion venom peptide Smp76 is a potential new antiviral agent with a unique mechanism involving type-Ⅰ IFN responses,demonstrating that natural AMPs can enhance immunity by functioning as immunomodulators. | Zhenglin Ji Fangfang Li Zhiqiang Xia Xingchen Guo Minjun Gao Fang Sun Yuting Cheng Yingliang Wu Wenxin Li Syed Abid Ali Zhijian Cao | 2018 | Virologica Sinica2018，33，6： | 2 |
| 2 | Nanopolyphenol rejuvenates microglial surveillance of multiple misfolded proteins through metabolic reprogramming显示文摘Microglial surveillance plays an essential role in clearing misfolded proteins such as amyloid-beta,tau,andα-synuclein aggregates in neurodegenerative diseases.However,due to the complex structure and ambiguous pathogenic species of the misfolded proteins,a universal approach to remove the misfolded proteins remains unavailable.Here,we found that a polyphenol,α-mangostin,reprogrammed metabolism in the disease-associated microglia through shifting glycolysis to oxidative phosphorylation,which holistically rejuvenated microglial surveillance capacity to enhance microglial phagocytosis and autophagy-mediated degradation of multiple misfolded proteins.Nanoformulation ofα-mangostin efficiently deliveredα-mangostin to microglia,relieved the reactive status and rejuvenated the misfolded-proteins clearance capacity of microglia,which thus impressively relieved the neuropathological changes in both Alzheimer’s disease and Parkinson’s disease model mice.These findings provide direct evidences for the concept of rejuvenating microglial surveillance of multiple misfolded proteins through metabolic reprogramming,and demonstrate nanoformulatedα-mangostin as a potential and universal therapy against neurodegenerative diseases. | Dayuan Wang Xiao Gu Xinyi Ma Jun Chen Qizhi Zhang Zhihua Yu Juan Li Meng Hu Xiaofang Tan Yuyun Tang Jianrong Xu Minjun Xu Qingxiang Song Huahua Song Gan Jiang Zaiming Tang Xiaoling Gao Hongzhuan Chen | 2023 | Acta Pharmaceutica Sinica B2023，13，2： | 1 |
| 3 | Ultraviolet-attenuated cercariae ofSchistosoma japonicum fail to effectively induce a Th1 response in spite of up-regulating expression of cytotoxicity-related genes in C57BL/6 mice显示文摘Objective:To better understand the reason that Schistosoma japonicum(S.japonicum)ultraviolet(UV)-radiated cercariae could not induce high level of protection in C57BL/6 mice.Methods:Microarray technology was performed to investigate the gene transcription profile in skin draining lymph nodes(sdLNs)at 1 w after exposure to attenuated cercariae(AC)or normal cercariae(NC)of S.japonicum in C57BL/6 mice.The expressions of some representative genes were further confirmed by real-time PCR.Subsequently,the expressions of Th1/Th2 cytokine genes,cytotoxicity-related genes,as well as co-stimulator genes in spleens from AC-vaccinated and NC- infected mice were analyzed by real-time PCR at w 3 and 6 post-exposure.Results:The gene expressions of Th1 cytokines,including interferon-γ(IFN-γ),interleukin(IL)-12 and tumor necrosis factor-α(TNF-α)in the sdLNs were significantly lower in AC-vaccinated mice than in NC-infected mice.Furthermore,the gene expressions of Th1-and Th2-cytokines,including IFN-γ,IL-12,TNF-α,IL-4 and IL-10,in the spleens from AC-vaccinated mice showed little changes at w 3 and 6 post-vaccination.In addition,cytotoxicity-related molecules including granzyme A,granzyme B,granzyme K,perforin 1 and Fas L were up-regulated from the early stage of vaccination,and peaked at the 3rd w after vaccination with UV-AC.Conclusion:UV-AC of S.japonicum could not effectively induce a Th1 response in C57BL/6 mice,which may be an explanation for the low protection against parasite challenge,and the role played by up-regulated expression of cytotoxicity-related genes in mice needs to be further investigated. | Meijuan Zhang Fang Tian Yanan Gao Minjun Ji Guanling Wu | 2010 | The Journal of Biomedical Research2010，24，4： | 0 |
| 4 | Wearable and stretchable conductive polymer composites for strain sensors:How to design a superior one?显示文摘Wearable and stretchable strain sensors have potential values in the fields of human motion and health monitoring,flexible electronics,and soft robotic skin.The wearable and stretchable strain sensors can be directly attached to human skin,providing visualized detection for human motions and personal healthcare.Conductive polymer composites(CPC)composed of conductive fillers and flexible polymers have the advantages of high stretchability,good flexibility,superior durability,which can be used to prepare flexible strain sensors with large working strain and outstanding sensitivity.This review has put forward a comprehensive summary on the fabrication methods,advanced mechanisms and strain sensing abilities of CPC strain sensors reported in recent years,especially the sensors with superior performance.Finally,the structural design,bionic function,integration technology and further application of CPC strain sensors are prospected. | Liwei Lin Sumin Park Yuri Kim Minjun Bae Jeongyeon Lee Wang Zhang Jiefeng Gao Sun Ha Paek Yuanzhe Piao | 2023 | Nano Materials Science2023，5，4： | 0 |
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