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| 1 | Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor显示文摘T 房间 receptor-CD3 建筑群(TCR ) 是能基于 CD3 细胞质的领域的各种各样的生物化学的变化开始抗原特定的有免疫力的回答的一台万用的发信号机器,但是内在的结构的基础留下逃犯。这里,我们开发了生物物理的途径学习 CD3 细胞质的领域的 conformational 动力学(CD3 CD ) 。在单个分子的水平,我们发现那 CD3 CD 能与三个功能的主题的不同坦诚有多重 conformational 状态,即, ITAM, BRS 和 PRS。因为,这些符合构造被产生 CD3 CD 有异构的类脂化合物绑定性质因此有异构的动力学。实时房间的成像实验证明不同抗原刺激能稳定 CD3 在不同符合构造的 CD 。类脂化合物依赖者 conformational 动力学因此为 TCR 的万用的发信号性质提供结构的基础。 | Xingdong Guo Chengsong Yan Hua Li Wenmao Huan Xiaoshan Shi Min Huang Yingfang Wang Weiling Pan Mingjun Cai Lunyi Li Wei Wu Yibing Bai Chi Zhang Zhijun Liu Xinyan Wang Xiaohui F Zhang Chun Tang Hongda Wang Wanli Liu Bo Ouyang Catherine C Wong Yi Cao Chenqi Xu | 2017 | Cell Research2017，27，4： | 5 |
| 2 | An atlas of CNV maps in cattle, goat and sheep显示文摘Copy number variation(CNV)is the most prevalent type of genetic structural variation that has been recognized as an important source of phenotypic variation in humans,animals and plants.However,the mechanisms underlying the evolution of CNVs and their function in natural or artificial selection remain unknown.Here,we generated CNV region(CNVR)datasets which were diverged or shared among cattle,goat,and sheep,including 886 individuals from 171 diverse populations.Using 9 environmental factors for genome-wide association study(GWAS),we identified a series of candidate CNVRs,including genes relating to immunity,tick resistance,multi-drug resistance,and muscle development.The number of CNVRs shared between species is significantly higher than expected(P<0.00001),and these CNVRs may be more persist than the single nucleotide polymorphisms(SNPs)shared between species.We also identified genomic regions under long-term balancing selection and uncovered the potential diversity of the selected CNVRs close to the important functional genes.This study provides the evidence that balancing selection might be more common in mammals than previously considered,and might play an important role in the daily activities of these ruminant species. | Yongzhen Huang Yunjia Li Xihong Wang Jiantao Yu Yudong Cai Zhuqing Zheng Ran Li Shunjin Zhang Ningbo Chen Hojjat Asadollahpour Nanaei Quratulain Hanif Qiuming Chen Weiwei Fu Chao Li Xiukai Cao Guangxian Zhou Shudong Liu Sangang He Wenrong Li Yulin Chen Hong Chen Chuzhao Lei Mingjun Liu Yu Jiang | 2021 | Science China(Life Sciences)2021，64，10： | 2 |
| 3 | Enhanced dSTORM imaging using fluorophores interacting with cucurbituril显示文摘Advanced fluorescence microscopy including single-molecule localization-based super-resolution imaging techniques requires bright and photostable dyes orproteins asfluorophores.The photophysical properties of fluorophores have been proven to be crucial for super-resolution microscopy's localization precision and imaging resolution.Fluorophores TAMRA and Atto Rho6 G,which can interact with macrocyclic host cucurbit[7]uril(CB7) to form host-guest compounds,were found to improve the fluorescence intensity and lifetimes of these dyes.We enhanced the localization precision of direct stochastic optical reconstruction microscopy(dSTORM) by introducing CB7 into the imaging buffer,and showed that the number of photons as well as localizations of both TAMRA and Atto Rho6 G increase over 2 times. | Min Zhang Jing Gao Junling Chen Mingjun Cai Junguang Jiang Zhiyuan Tian Hongda Wang | 2016 | Science China Chemistry2016，59，7： | 1 |
| 4 | A Hu sheep genome with the first ovine Y chromosome reveal introgression history after sheep domestication显示文摘The Y chromosome plays key roles in male fertility and reflects the evolutionary history of paternal lineages.Here,we present a de novo genome assembly of the Hu sheep with the first draft assembly of ovine Y chromosome(o MSY),using nanopore sequencing and Hi-C technologies.The o MSY that we generated spans 10.6 Mb from which 775 Y-SNPs were identified by applying a large panel of whole genome sequences from worldwide sheep and wild Iranian mouflons.Three major paternal lineages(HY1a,HY1b and HY2)were defined across domestic sheep,of which HY2 was newly detected.Surprisingly,HY2 forms a monophyletic clade with the Iranian mouflons and is highly divergent from both HY1a and HY1b.Demographic analysis of Y chromosomes,mitochondrial and nuclear genomes confirmed that HY2 and the maternal counterpart of lineage C represented a distinct wild mouflon population in Iran that diverge from the direct ancestor of domestic sheep,the wild mouflons in Southeastern Anatolia.Our results suggest that wild Iranian mouflons had introgressed into domestic sheep and thereby introduced this Iranian mouflon specific lineage carrying HY2 to both East Asian and Africa sheep populations. | Ran Li Peng Yang Ming Li Wenwen Fang Xiangpeng Yue Hojjat Asadollahpour Nanaei Shangquan Gan Duo Du Yudong Cai Xuelei Dai Qimeng Yang Chunna Cao Weidong Deng Sangang He Wenrong Li Runlin Ma Mingjun Liu Yu Jiang | 2021 | Science China(Life Sciences)2021，64，7： | 1 |
| 5 | Effects of RNAi-mediated gene silencing of LRIG3 expression on cell cycle and survival of glioma cells显示文摘 | Mingjun Cai Ruifan Xie Lin Han Rudong Chen Baofeng Wang Fei Ye Dongsheng Guo Ting Lei | 2009 | Journal of Huazhong University of Science and Technology2009，，1： | 1 |
| 6 | Estimation of Lightning-Generated NO_(x) in the Mainland of China Based on Cloud-to-Ground Lightning Location Data显示文摘Lightning-generated nitrogen oxides(LNO_(x))have a major influence on the atmosphere and global climate change.Therefore,it is of great importance to obtain a more accurate estimation of LNO_(x).The aim of this study is to provide a reference for the accurate estimation of the total LNO_(x) in the mainland of China based on cloud-to-ground lightning(CG)location data from 2014 to 2018.The energy of each CG flash was based on the number of return strokes per CG flash,the peak current of each return stroke,and the assumed CG breakdown voltage.The energy of intracloud lightning(IC)was based on the estimated frequencies of IC and the assumed energy of each IC flash.Combining the energy of lightning and the number of nitric oxide(NO)molecules produced by unit energy(ρno),the total LNO_(x) production in the mainland of China was determined.The LNO_(x) in the mainland of China estimated in this study is in the range(0.157-0.321)×10^(9) kg per year[Tg(N)yr-1],which is on the high end of other scholars’works.Negative cloud-to-ground lightning(NCG)flashes produce the most moles of NO_(x),while positive cloud-to-ground lightning(PCG)flashes produce the least total moles of NO_(x).The breakdown voltage of PCG is greater than that of IC or NCG,while the latter has a greater output of LNO_(x). | Qi LI Fengxia GUO Xiaoyu JU Ze LIU Mingjun GAN Kun ZHANG Binbin CAI | 2023 | Advances in Atmospheric Sciences2023，40，1： | 0 |
| 7 | Mechanistic Insights into Membrane Protein Clustering Revealed by Visualizing EGFR Secretion显示文摘Most plasmalemmal proteins are organized into clusters to modulate various cellular functions.However,the machineries that regulate protein clustering remain largely unclear.Here,with EGFR as an example,we directly and in detail visualized the entire process of EGFR from synthesis to secretion onto the plasma membrane(PM)using a high-speed,high-resolution spinning-disk confocal microscope.First,colocalization imaging revealed that EGFR secretory vesicles underwent transport from the ER to the Golgi to the PM,eventually forming different distribution forms on the apical and basal membranes;that is,most EGFR formed larger clusters on the apical membrane than the basal membrane.A dynamic tracking image and further siRNA interference experiment confirmed that fusion of secretory vesicles with the plasma membrane led to EGFR clusters,and we showed that EGFR PM clustering may be intimately related to EGFR signaling and cell proliferation.Finally,we found that the size and origin of the secretory vesicles themselves may determine the difference in the distribution patterns of EGFR on the PM.More importantly,we showed that actin influenced the EGFR distribution by controlling the fusion of secretory vesicles with the PM.Collectively,a comprehensive understanding of the EGFR secretion process helps us to unravel the EGFR clustering process and elucidate the key factors determining the differences in the spatial distribution of EGFR PM,highlighting the correlation between EGFR secretion and its PM distribution pattern. | Haijiao Xu Jinrui Zhang Yijia Zhou Guanfang Zhao Mingjun Cai Jing Gao Lina Shao Yan Shi Hongru Li Hongbin Ji Yikai Zhao Hongda Wang | 2023 | Research2023，，1： | 0 |
| 8 | Effect of laser-discrete-quenching on bonding properties of electroplated grinding wheel with AISI 1045 steel substrate and nickel bond显示文摘To improve the bonding strength between the nickel bond and the hub of the electroplated diamond grinding wheel,a hybrid technique was proposed to combine laser prequenching steel substrate and post-electroplating nickel.To validate the effectiveness of the proposed technique,AISI 1045 substrate was nickel-coated.The bonding properties between the electroplated nickel coating and substrate with or without laser-discrete-quenching were discussed comparatively by scratch,indentation,and thermal shock tests.The results show that the prequenching treatment leads to phase transformation of AISI 1045 microstructure from the mixed pearlite and ferrite phases into the martensitic phase.Since the martensitic phase is characterized as a high corrosion resistance,the interface of substrate/coating is smooth and flat in the prequenched zone,and the coating is bonded well with the steel substrate.In contrast to the steel substrate without pre-quenching treatment,the proposed technique significantly enhanced the bonding strengths of the electroplated nickel-coating.On one hand,the average hardness of electroplated nickel-coating on the laser pre-quenched zone is increased by 18.7%,and the scratch depth with the same load become narrower and shallower.On the other hand,the coefficient of friction(CoF)and the vibration amplitude are reduced,and the coating is bonded effectively with the substrate to inhibit the crack initialization at the interface.This prevents effectively the coating from peeling off and improves significantly the thermal shock resistance property. | Cong MAO Peihao CAI Yongle HU Yujie ZHONG Jishuang WEI Zhuming BI Yifeng JIANG Linfeng XIAO Mingjun ZHANG | 2021 | Chinese Journal of Aeronautics2021，34，6： | 0 |
| 9 | Combining methylated SEPTIN9 and RNF180 plasma markers for diagnosis and early detection of gastric cancer显示文摘Dear Editor,Early diagnosis is critical for successful treatment of gastric adenocarcinoma(GA).However,the sensitivities of tumor markers carcinoembryonic antigen(CEA),cancer antigen 19-9(CA19-9)and CA72-4 for GA detection are approximately 20%[1],and the sensitivities of all markers combined for early gastric cancer detection is still very low[2].DNA methylation plays a major role in tumorigenesis and therefore has obvious potential as a non-invasive biomarker for cancer detection[3].Through genome-wide methylation analysis and histological verification,we previously identified ring finger protein 180(RNF180)as a novel preferentially methylated gene in GA[4,5]. | Yongzhan Nie Xianchun Gao Xiqiang Cai Zhen Wu Qiaoyi Liang Guobing Xu Na Liu Peng Gao Jingyu Deng Hongzhi Xu Zhanlong Shen Changqi Cao Fenrong Chen Nannan Zhang Yongxi Song Mingjun Sun Chengyin Liu Guangpeng Zhou Weili Han Jianhua Dou Huahong Xie Liping Yao Zhiguo Liu Gang Ji Xin Wang Qingchuan Zhao Lei Shang Daiming Fan Xiaoliang Han Jianlin Ren Han Liang Zhenning Wang Jinhai Wang Qi Wu Jun Yu Kaichun Wu the MAGIS Study Group | 2023 | Cancer Communications2023，43，11： | 0 |
| 10 | Cell membrane sample preparation method of combined AFM and dSTORM analysis显示文摘A major role of cell membranes is to provide an ideal environment for the constituent proteins to perform their biological functions.A deep understanding of the membrane proteins assembly process under physiological conditions is quite important to elucidate both the structure and the function of the cell membranes.Along these lines,in this work,a complete workflow of the cell membrane sample preparation and the correlated AFM and dSTORM imaging analysis methods are presented.A specially designed,angle-controlled sample preparation device was used to prepare the cell membrane samples.The correlated distributions of the specific membrane proteins with the topography of the cytoplasmic side of the cell membranes can be obtained by performing correlative AFM and dSTORM measurements.These methods are ideal for systematically studying the structure of the cell membranes.The proposed method of the sample characterization was not only limited to the measurement of the cell membrane but also can be applied for both biological tissue section analysis and detection. | Mingjun Cai Huili Wang Guanfang Zhao Hongru Li Jing Gao Hongda Wang | 2022 | Biophysics Reports2022，8，4： | 0 |
| 11 | Regulation of EGFR nanocluster formation by ionic protein-lipid interaction显示文摘表皮的生长因素受体(EGFR ) 的反常激活强烈与许多人的癌症被联系,但是内在的分子的机制充分没被理解。由使用直接随机的光重建显微镜学(dSTORM ) ,我们发现 EGFR 蛋白质在正常的肺的房间膜形成 nanoclusters 簇的上皮的房间和肺癌症房间,而是数字和尺寸显著地在肺癌症房间增加。EGFR 簇的形成被在 anionic 类脂化合物 phosphatidylinositol-4,5-bisphosphate (PIP2 ) 之间的离子的相互作用在血浆膜和 juxtamembrane (JM ) 调停 EGFR 的区域。由 PIP2 弄空或 JM 区域变化聚类的 EGFR 的混乱损害 EGFR 激活并且下游的发信号。而且,在组成地活跃的 EGFR 异种的 JM 区域变化稀释它房间转变的能力。一起,我们的调查结果在 EGFR 发信号和功能加亮 anionic phospholipids 的关键角色,并且揭示新奇机制在癌症解释 EGFR 的异常激活。 | Ye Wang Jing Gao Xingdong Guo Ti Tong Xiaoshan Shi Lunyi Li Miao Qi Yajie Wang Mingjun Cai Junguang Jiang Chenqi Xu Hongbin Ji Hongda Wang | 2014 | Cell Research2014，24，8： | 0 |
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