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| 1 | Ganoderma Lucidum polysaccharide peptides protect kidney from renal ischemia reperfusion injury via counteracting oxidative stress显示文摘OBJECTIVE Ganoderma lucidum polysaccharide peptides(GLPP)have an anti-oxidant activity.The oxidative stress implicates in the pathogenesis of renal ischemia-reperfusion injury(RIRI).The objective of this study was to determine whether GLPP could attenuate RIRI via counteracting the oxidative stress.METHODS Mice subjected to uninephrectomy with the right kidney ischemia for 35 min and reperfusion for 24 hwere used to explore the protective activity of GLPP against RIRI.In GLPP-treated group,100mg·kg-1·d-1 of GLPP were intraperitoneally injected for 7dbefore the procedure.In vitro,NRK-52 Ecells subjected to hypoxia-reoxygenation(H/R)and tunicamycin were used to explore the protective effect of GLPP against oxidative stress.The mechanisms in which GLPP protected kidney from RIRI were studied using a series of physiological and molecular biological methods.RESULTS Kidneys undergone ischemia-reperfusion showed renal dysfunction and characteristic morphological changes including cellular necrosis,brush border loss,cast formation,vacuolization and tubular dilatation while these damages were significantly attenuated by GLPP treatment.The abnormal levels of MPO,MDA and SOD caused by renal ischemia-reperfusion were significantly reversed by GLPP treatment.More apoptotic cells were found in the renal ischemia-reperfusion group than the sham group whereas GLPP reduced apoptotic cells in the ischemia-reperfusion mice by21.75%(P<0.01).The GLPPs(25-1μg·mL)alleviated H/R induced cell viability loss by 20.12%(P<0.01)andΔφm dissipation by 27.3%(P<0.01)in vitro as well and its pretreatment dramatically reduced H/R and tunicamycin induced cell injury.CONCLUSION Our study found that GLPP had a protective effect on RIRI via its anti-oxidative capacity,which suggests that GLPP may be developed as a candidate drug for preventing acute kidney injury. | Dan-danZHONG Hong-kaiWANG MingLIU Ying-liJIA MingHUANG HongZHOU Shu-qianLIN Zhi-binLIN Bao-xueYANG | 2015 | 中国药理学与毒理学杂志2015，29，S1： | 21 |
| 2 | New ommatids from the Late Jurassic of western Liaoning, China (Coleoptera: Archostemata)显示文摘A new genus Amblomma gen. nov. of fossil beetles is erected and can be assignedto the family Ommatidae because its two procoxal cavities are contiguous and the articulations of the abdominal ventrites are abutting. The new genus is similar to Zygadenia Handlirsch, 1906 (=Notocupes Ponomarenko, 1964), Tetraphalerus Waterhouse, 1901,Rhobdocupes Ponomarenko, 1966 and Sinocupes Lin, 1976, but can be distinguished from other genera according to the following characters: the second segment of antennae is shorterthan the third one in length; the posterior tarsi with the basal segment is obviously shorter than the three following taken together in length; the antennae reach the posterior ridge of prothroax in length, and the sides of the prothroax with serrulate margin. Four new species of the new genus are described and figured: Amblomma psilata gen. et sp. nov., Amblomma rudis gen. et sp. nov., Amblomma epicharis gen. et sp. nov., and Amblomma stabilis gen. etsp. nov. A key to species within this new genus is provided. All the specimens are collected from the Late Jurassic Yixian Formation of western Liaoning and are now housed in the College of Life Science, Capital Normal University, Beijing, China. | JING-JINGTAN DONGREN MINGLIU | 2005 | Insect Science2005，12，3： | 8 |
| 3 | A COMPLETE SCREEN FOR MUTATIONS OF THE RHODOPSIN GENE IN A PANEL OF CHINESE PATIENTS WITH AUTOSOMAL DOMINANT RETINITIS PIGMENTOSA显示文摘Objective To evaluate the prevalence of rhodopsin (RHO) mutations and the genotype-phenotype relationships in Chinese patients with autosomal dominant retinitis pigmentosa (ADRP) by conformation sensitive gel electrophoresis (CSGE) and direct DNA sequencing. Methods We have screened the five coding exons and splice sites of RHO gene in 27 probands who had no relativity from Chinese ADRP families and 100 normal controls to identify disease-associated mutations, using CSGE and direct DNA sequencing. Family members of some probands with disease-associated mutations were also genotyped to determine whether the RHO mutations segregated with retinitis pigmentosa (RP) in their families. Results Two RHO mutations, Pro347Leu and Pro327 (1-bp del), were identified separately in two families, thus the frequency of RHO mutations among this set of Chinese ADRP families is about 7.4% (2/27). Pro347Leu mutation was found in one ADRP proband as well as three her children who also had RP. She had relatively early onset at about 17 years. The only one child without this mutation had no symptom or sign of RP at age of 34. Pro327 (1-bp del) was identified in a late-onset ADRP patient, who appeared night blindness around 30 years old and in her fifties electroretinogram (ERG) has been flat in both scotopic and photopic phases. Family analysis showed that this mutation also existed in her younger dau-ghter and her elder sister, both of them also had RP. Three other family members were genotypically and phenotypically normal. Neither of the two mutations was detected in 100 normal controls.Conclusions The frequency of RHO mutations in Chinese patients was lower than that in Europe and North America. The phenotype of the patients with Pro347Leu corresponded to type 1 ADRP, with severe rod degeneration and some cone preservation later, while the phenotype of the patients carrying Pro327 (1-bp del) corresponded to type 2 ADRP, with a concomitant loss of rod and cone visual function. CSGE was found to be a sensitive, simple, and practical method for the screening of a large number of samples under highly reproducible conditions, and could be utilized in routine molecular diagnostic laboratories. | Xiao-liZhang MingLiu Xiao-hongMeng Wei-lingFu Zheng-qinYin XueZhang Jun-fuHuang | 2005 | Chinese Medical Sciences Journal2005，20，1： | 7 |
| 4 | Reversal of 5-flouroucial resistance by adenovirus-mediated transfer of wild-type p53 gene in multidrug-resiatant human colon carcinoma LoVo/5-FU cells显示文摘AIM: To observe the reversal effects of wide-type p53 gene on multi-drug resistance to 5-FU (LOVO/5-FU).METHODS: After treatment with Ad-p53, LOVO/5-FU sensitivity to 5-Fu was investigated using tetrazolium dye assay. Multidrug resistance gene-1 (MDR1) gene expression was assayed by semi-quantitative reverse transcriptionpolymerase chain reaction and the expression of p53 protein was examined by Western blotting.RESULTS: The reversal activity after treatment with wide type p53 gene was increased up to 4.982 fold at 48 h. The expression of MDR1 gene decreased significantly after treatment with wide-type p53 gene, and the expression of p53 protein lasted for about 5 d, with a peak at 48 h, and began to decrease at 72 h.CONCLUSION: Wide-type p53 gene has a remarkable reversal activity for the high expression of MDR1 gene in colorectal cancers. The reversal effects seem to be in a time dependent manner. It might have good prospects in clinical application. | Zhi-WeiYu PengZhao MingLiu Xin-ShuDong JiTao Xue-QinYao Xin-ttuaYin YuLi Song-BinFu | 2004 | World Journal of Gastroenterology2004，10，13： | 7 |
| 5 | Predictive value of the fraction of cancer cells immunolabeled for proliferating cell nuclear antigen or Ki67 in biopsies of head and neck carcinomas to identify lymph node metastasis: Comparison with clinical and radiologic examinations显示文摘 | MingLiu GeorgesLawson MoniqueDelos JacquesJamart ChristopheIde EmmanuelCoche BirgitWeynand GauthierDesuter MarcHamoir MarcRemacle EtienneMarbaix | 2003 | Head Neck2003，，4： | 3 |
| 6 | Developmentofcompetencyinventoryforregisterednursesinthepeople’srepublicofChina:Scaledevelopment显示文摘 | MingLiu WipadaK WilawanS etal | 2007 | InternationalJournalofNursingStudies2007，44，3： | 1 |
| 7 | Identification of common pathways mediating differentiation of bone marrow-and adipose tissue-derived human mesenchymal stem cells into three mesenchyma! lineages显示文摘 | Tong Mingliu Monique Martina Dietmar W Hutmacher | 2007 | Stem Cell2007，25，： | 1 |
| 8 | Research on the control laws of the electronic expansion valve for an air source heat pump water heater显示文摘 | Jiang Mingliu Wu Jingyi Wang Ruzhu | 2011 | Building and Environment2011，46，10： | 1 |
| 9 | Determination of catecholamines by CE with direct chemiluminescence detection显示文摘 | Yan‐MingLiu Cheng‐QuanWang Hai‐BeiMu Jun‐TaoCao Yan‐LiZheng | 2007 | ELECTROPHORESIS2007，，12： | 1 |
| 10 | α‐glucosidase inhibition and the in vivo hypoglycemic effect of butyl‐isobutyl‐phthalate derived from the Laminaria japonica rhizoid显示文摘 | TongBu MingLiu LanhongZheng YueweiGuo XiukunLin | 2010 | Phytother Res2010，，11： | 1 |
| 11 | Identification of Common Pathways Mediating Differentiation of Bone Marrow‐ and Adipose Tissue‐Derived Human Mesenchymal Stem Cells into Three Mesenchymal Lineages显示文摘 | Tong MingLiu MoniqueMartina Dietmar W.Hutmacher James Hoi PoHui Eng HinLee BingLim | 2009 | STEM CELLS2009，，3： | 1 |
| 12 | Research on refrigerant flow characteristics and performance of a multi-functional heat pump system显示文摘 | Jiang Mingliu Wu Jingyi Wang Ruzhu | 2011 | Energy Conversion and Management2011，52，6： | 1 |
| 13 | Perturbation analysis of the generalized Bott-Duffin inverse of L-zero matrices显示文摘 | CHEN Guoliang GUO Mingliu Xue Yifeng | 2003 | Linear Multilinear Algebra2003，51，： | 1 |
| 14 | Antagonistic Activities of Lactobacilli against Helicobacter pylori Growth and Infection in Human Gastric Epithelial Cells显示文摘 | XiaohuaChen Xiao‐mingLiu FengweiTian QiuxiangZhang He‐pingZhang HaoZhang WeiChen | 2012 | Journal of Food Science2012，，1： | 1 |
| 15 | Research on the control laws of the electronic expansion valve for an air source heat pump water heater显示文摘 | Mingliu Jiang Jingyi Wu Ruzhu Wang Yuxiong Xu | 2011 | Building and Environment2011，，10： | 1 |
| 16 | Estimation of Docosahexaenoic Acid Glycerides in Schizochytrium spOil by Open Column Chromatography and Reversed- phase HPLC显示文摘 | Jiang Lu Huang He Lei Mingliu | 2013 | Journal of the American Oil Chemists'' Society2013，90，8： | 1 |
| 17 | Protective effects of NYG-1 on CCl4-induced acute liver injury in rats显示文摘OBJECTIVE To study the protection effects and mechanisms of NYG-1 on CCl4-induced acute liver injury.METHODS Acute liver injury model of rats was established by using CCl4.48 male SPF SD rats were weighed and randomly divided into six groups with 8 in each group,normal group,model group,positive control group(silibinin),low-,medium-and high-dose NYG-1 group.Silibinin was given orally to rats in the positive control group,NYG-1(high-,medium-and low-dose)was given orally in the high-,medium-and low-dose NYG-1group,respectively.Those rats were administered appropriately according to the group once daily for seven consecutive days.On the seventh day,rats were treated with 10% CCl4(10mL·kg-1 of0.1% CCl4 solution in olive oil)intraperitoneally injecting(ip)to induce acute liver injury,except the normal group.At 16 h after CCl4 treatment,rats were weighed,then anaesthed with ether,the blood and liver were collected.Serum ALT,AST,LDH and ALP were measured.MDA content and SOD activity in liver homogenate were detected.The histopathological changes of liver were observed by H&E staining.RESULTS Acute liver injury model was established successfully in rats by intraperitoneally injecting CCl4.Pretreatment with medium and high dose NYG-1 decreased the increase of ALT,AST and MDA induced by CCl4,but it had no influence on serum LDH,ALP level and SOD activity in the liver homogenate.CONCLUSION The obtained results suggest that oral administration of NYG-1 hasve the protective effects against CCl4-induced acute hepatic injury in rats,Its mechanism may be related to antioxidant-like action. | Hong-liangLI Xuan-binWANG MingLIU Yi-binFENG Qiu-fangZHANG | 2015 | 中国药理学与毒理学杂志2015，29，S1： | 0 |
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