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| 1 | HCV-RNA positivity in peripheral blood mononuclear cells of patients with chronic HCV-infection: does it really mean viral replication?显示文摘AIM To analyze the association of HCV-RNA with peripheral blood mononuclear cells (PBMC)and to answer the question whether HCV-RNA positivity in PBMC is due to viral replication,METHODS HCV-RNA was monitored in serumand PBMC preparations from 15 patients with chronic HCV infection before, during and after an IFN-α therapy using a nested RT/ PCRtechnique. In a second approach, PBMC from healthy donors were incubated in HCV positive plasma.RESULTS In the IFN-α responding patients,HCV-RNA disappeared first from total RNApreparations of PBMC and then from serum. In contrast, in relapsing patients, HCV-RNAreappeared first in serum and then in PBMC. A quantitative analysis of the HCV-RNAconcentration in serum was performed before and after transition from detectable to nondetectable HCV-RNA in PBMC-RNA and vice versa. When HCV-RNA was detectable in PBMCpreparations, the HCV concentration in serum was significantly higher than the serum HCV-RNA concentration when HCV-RNA in PBMC was not detectable. Furthermore, at no time during the observation period was HCV specific RNA observed in PBMC, if HCV-RNA in serum was under the detection limit. Incubation of PBMCfrom healthy donors with several dilutions of HCV positive plasma for two hours showed a concentration-dependent PCR-positivity for HCV-RNA in reisolated PBMC.CONCLUSION The detectability of HCV-RNA in total RNA from PBMC seems to depend on the HCV concentration in serum. Contamination or passive adsorption by circulating virus could be the reason for detection of HCV-RNA in PBMCpreparations of chronically infected patients. | Volker Meier Sabine Mihm Perdita Wietzke-Braun Guliano Ramadori | 2001 | World Journal of Gastroenterology2001，7，2： | 29 |
| 2 | Diet high in fructose leads to an overexpression of lipocalin-2 in rat fatty liver显示文摘AIM:To explore lipocalin-2(LCN-2)expression and its possible role and mechanism(s)of production in rat models of diet-inducible fatty liver.METHODS:Fatty liver was triggered in male SpragueDawley rats fed either with liquid Lieber-DeCarli(LDC)or LDC+70%cal fructose(L-HFr)diet for 4 or 8 wk.Chow-nourished animals served as controls.Hepatic expression of LCN-2 and other metabolic and inflammatory mediators was assessed by quantitative reverse transcription polymerase chain reaction and Western blotting.Serum LCN-2,fasting leptin,and lipid profile were evaluated via Enzyme-Linked Immunosorbent Assay,Radioimmunoassay,and colorimetric assays,respectively.The localization of LCN-2 in the liver was detected by using immunofluorescence staining.Furthermore,HE stain was used to evaluate hepatic fatdegeneration and inflammation.RESULTS:Both LDC-fed and L-HFr-fed rat histologically featured fatty liver.In the liver,mRNA transcriptions of Mcp-1,a2-m,Il-8 and Glut5 were increased in the L-HFr group at both time points(P<0.001),while the transcription of Tlr4,Inos,and Tnf-a was significantly up-regulated at week 4.Interestingly,hepatic Lcn-2 expression was 90-fold at week 4 and 507-fold at week 8 higher in L-HFr-subjected rats vs control(P<0.001).In contrast to HDL-cholesterol,systemic levels of LCN-2,fasting leptin and triglycerides were elevated in the L-HFr regimen(P<0.001).Moreover,protein expression of hepatic LCN-2,CD14,phosphoMAPK,caspase-9,cytochrome c and 4-hydroxynonenal was increased in the L-HFr group.Conversely,the hepatic expression of PGC-1a(a mitochondrial-biogenic protein)was reduced in the L-HFr category at week 8.The localization of LCN-2 in the liver was predominantly restricted to MPO+granulocytes.CONCLUSION:Fructose diet up-regulates hepatic LCN-2 expression,which correlates with the increased indicators of oxidative stress and mitochondrial dysfunction.The LCN-2 may be involved in liver protection. | Salamah Mohammad Alwahsh Min Xu Hatice Ali Seyhan Shakil Ahmad Sabine Mihm Giuliano Ramadori Frank Christian Schultze | 2014 | World Journal of Gastroenterology2014，20，7： | 7 |
| 3 | 脉冲染料激光联合外用咪喹莫特与单纯激光治疗鲜红斑痣的初步对比研究显示文摘研究目的评估脉冲染料激光(pulsed dye laser PDL)联合外用咪喹莫特治疗与单纯激光治疗鲜红斑痣的疗效。研究设计对20名亚裔鲜红斑痣受试者的结果进行评估。鲜红斑痣皮损内设置三个治疗测试区:A区用PDL+外用咪喹莫特治疗;B区单纯PDL治疗;C区单纯外用咪喹莫特软膏治疗。治疗后1、3、6和12个月进行随访,利用皮肤光谱检测仪测量血红蛋白指数。 | 苑凯华 Chang CJ Hsiao YC Mihm MC | 2011 | 中国激光医学杂志2011，20，1： | 6 |
| 4 | Impaired Myofibrillar Energetics and Oxidative Injury During Human Atrial Fibrillation显示文摘 | Michael J. Mihm Fushun Yu Cynthia A. Carnes Peter J. Reiser Patrick M. McCarthy David R. Van Wagoner John Anthony Bauer | 2001 | Circulation: Journal of the American Heart Association2001，，2： | 2 |
| 5 | Endotoxin receptor CD14 gene variants and histological features in chronic HCV infection显示文摘AIM:To analyze the correlation between CD14 rs2569190/C-159T single nucleotide polymorphism (SNP) and disease progression in chronic hepatitis C.METHODS: Liver biopsy specimens from a total of 137 and 349 patients with chronic hepatitis C were separately evaluated with respect to necroinflammatory activity (grading) and architectural changes (staging). In one group, further histological lesions characteristic for hepatitis C, hepatitis C virus subtypes, and biochemical parameters of liver disease were also investigated. Samples of genomic DNA were genotyped for the respective SNP by 5'-nuclease assays using fluorescent dye-labeled allele-specif ic probes.RESULTS: Genotype distribution did not deviate from the Hardy-Weinberg equilibrium. In the first group, patients homozygous for the variant allele T were found to be younger than C allele carriers (39.6±12.5 vs 45.7±11.5, P=0.008). Among the histological lesions studied, portal lymphoid aggregates were more frequently observed among TT homozygotes than among C carriers (21/37 vs 32/100, P=0.008). The presence of portal lymphoid aggregates was closely correlated with hepatic inflammation (P=0.003) and with bile duct damage (P<0.001). The degree of fibrosis, in contrast, was not found to be related to the CD14 gene C-159T polymorphism.CONCLUSION: The data suggest a possible relationship between CD14 C-159T polymorphism and the formation of portal lymphoid aggregates, but not liver fibrosis progression in chronic hepatitis C. | Eva Askar Giuliano Ramadori Sabine Mihm | 2009 | World Journal of Gastroenterology2009，15，31： | 2 |
| 6 | Spontaneous elimination of hepatitis C virus infection: A retrospective study on demographic, clinical, and serological correlates显示文摘AIM: To fi nd correlates to spontaneous clearance of hepatitis C virus (HCV) infection, this study compared individuals with self-limited and chronic infection with regard to clinical, demographic, and serological pa-rameters. METHODS: Sixty-seven anti-HCV positive and repeatedly HCV RNA negative individuals were considered to have resolved HCV infection spontaneously. To determine the viral genotype these patients had been infected with HCV serotyping was performed. For comparison reasons, 62 consecutive patients with chronic hepatitis C were enrolled. Cases and controls were compared stratifi ed for age and sex. RESULTS: Retrospective analysis showed (1) a lower humoral reactivity to HCV in patients with self-limited compared to chronic HCV-infection and (2) that younger age, history of iv drug use, and acute/post-acute hepatitis A or B co-infections, but not viral genotypes, are independent correlates for spontaneous HCV clearance. CONCLUSION: The stronger humoral reactivity to HCV in patients with persistent infections and in those with a history of iv drug use is supposed to be due to continuous or repeated contact(s) to the antigen. Metachronous hepatitis A or hepatitis B infections might favor HCV clearance. | Perdita Wietzke-Braun Larissa Bettina Mnhardt Albert Rosenberger Angela Uy Giuliano Ramadori Sabine Mihm | 2007 | World Journal of Gastroenterology2007，13，31： | 2 |
| 7 | Impaired myofibrillar energetics and oxidative injury during human atrial fibrillation显示文摘 | Mihm MJ Yu F Carnes CA | 2001 | Circulation2001，10，4： | 1 |
| 8 | Kimura's disease and angiolymphoid hyperplasia with eosinophilia: two distinct histopathological entitie显示文摘 | Googe PB Harris NL Mihm MC Jr | 1987 | Cutan Pathol1987，14，： | 1 |
| 9 | Cardiac peroxynitrite formation and left ventricular dysfunction following doxorubicin treatment in mice显示文摘 | WEINSTEIN D M MIHM M J BAUER J A | 2000 | J Pharmacol Exp Ther2000，294，1： | 1 |
| 10 | Laparoscopic management of bladder pheochromocytoma显示文摘 | Kozlowski PM Mihm F Winfield HN | 2001 | Urology2001，，02： | 1 |
| 11 | Preferential virological response to interferon-α 2a in patients with chronic hepatitis C infected by virus genotype 3a and exhibiting a lowγ-GT/ALT ratio显示文摘 | Sabine Mihm PhD Heinz Hartmann MD Afshin Fayyazi MD Prof. Dr. Giuliano Ramadori MD | 1996 | Digestive Diseases and Sciences1996，，6： | 1 |
| 12 | Problem-Solving Oscillations in Complex Engineering Projects显示文摘 | Mihm J Loch C Huchzermeier A | 2003 | Management Science2003，49，6： | 1 |
| 13 | Squamoid eccrine ductal carcinoma显示文摘 | Wong TY Suster S Mihm MC | 1997 | Histopathology1997，30，3： | 1 |
| 14 | Optimal therapy in genotype 1 patients显示文摘 | Farnik H Mihm U Zeuzem S | 2009 | Liver Int2009，29，1： | 1 |
| 15 | Incentives in New Product Development Projects and the Role of Target Costing显示文摘 | Mihm J | 2010 | Management Science2010，56，8： | 1 |
| 16 | Peroxynitrite inducednitration and inactivation of myofibrillar creatine kinase in experimental heart failure 显示文摘 | MIHM M J COYLE C M SCHANBACHER B L | 2001 | Cardiovasc Res2001，49，4： | 1 |
| 17 | Vaccination with irradiated autologous melanoma cells engineered to secrete human granulocyte-macroghage colony-stimulating factor generates potent antitumor immunity in patients with metastatic melanoma显示文摘 | Soiffer R Lynch T Mihm M | 1998 | Proc Natl Acad Sci USA1998，95，13： | 1 |
| 18 | Current theories on the pathogenesis of infan- tile hemangioma 显示文摘 | Lo K Mihm M Fay A | 2009 | Semin Ophthalmol2009，24，3： | 1 |
| 19 | Interferon type Ⅰ gene expression in chronic hepatitis C显示文摘 | Mihm S Frese M Meier V | 2004 | Lab Invest2004，84，9： | 1 |
| 20 | Association between the duration of dominance of the ovulatory follicle and pregnancy rate in beef heifers 显示文摘 | Mihm M Baguisi A Boland M P | 1994 | Reprod Fertil1994，102，： | 1 |
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