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| 1 | Glutamate synthase 1 is involved in iron-deficiency response and long-distance transportation in Arabidopsis显示文摘Iron is an essential microelement for plant growth.After uptake from the soil,iron is chelated by ligands and translocated from roots to shoots for sub-sequent utilization.However,the number of ligands in-volved in iron chelation is unclear.In this study,we identified and demonstrated that GLU1,which encodes a ferredoxin-dependent glutamate synthase,was involved in iron homeostasis.First,the expression of GLU1 was strongly induced by iron deficiency condition.Second,lesion of GLU1 results in reduced transcription of many iron-deficiency-responsive genes in roots and shoots.The mutant plants revealed a decreased iron concentration in the shoots,and displayed severe leaf chlorosis under the condition of Fe limitation,compared to wild-type.Third,the product of GLU1,glutamate,could chelate iron in vivo and promote iron transportation.Last,we also found that supplementation of glutamate in the medium can alleviate cadmium toxicity in plants.Overall,our results provide evidence that GLU1 is involved in iron homeo-stasis through affecting glutamate synthesis under iron deficiency conditions in Arabidopsis. | Man Cui Mengjun Gu Yaru Lu Yue Zhang Chunlin Chen Hong‐Qing Ling Huilan Wu | 2020 | Journal of Integrative Plant Biology2020，62，12： | 2 |
| 2 | Synthesis and biological evaluation of ^(18)F-FB-NGA as a hepatic asialoglycoprotein receptor PET imaging agent显示文摘Asialoglycoprotein receptor(ASGP-R)is a hepatic membrane receptor that uniquely exists on the surface of mammalian hepatocytes,and has been used as target of liver functional imaging agents for many years.We labeled the Galactosyl-neoglycoalbumin(NGA)with 18F to get a PET molecular probe 18F-FB-NGA and evaluated its ability as a liver functional PET imaging agent.The 18F-FB-NGA was prepared with NGA by conjugation with Nsuccinimidyl-4-18F-fluorobenzoate(18F-SFB)and purified with PD-10 desalting column.The radiolabeling yield and radiochemical purity of 18F-FB-NGA were determined by radio-HPLC.Starting with 18F-F–,the total time for 18F-FB-NGA was about 120±10 min.The decay-corrected radiochemical yield is about 25–30%.The radiochemical purity of purified 18F-FB-NGA was more than 98%.Labeled with 185–1850 MBq 18F-SFB,the specific activity of 18F-FBNGA was estimated to be 7.83–78.3 TBq/mmol.Biodistribution of 18F-FB-NGA in normal mice was investigated after injection through the tail vein.The results showed that the liver accumulated 39.47±3.42 and 12.12±6.11%ID/g at 10 and 30 min after injection,respectively.Dynamic MicroPET images in mice were acquired with and without block after injection of the radiotracer,respectively.High liver activity accumulation was observed at 5 min after injection in normal group.On the contrary,the liver accumulation was significantly lower after block,indicating the specific binding to ASGP-R.18F-FB-NGA is probably a potential PET liver imaging agent. | GU Xiaobo CAI Gangming JIANG Mengjun ZHOU Yaoyuan ZHANG Rongjun | 2013 | Nuclear Science and Techniques2013，24，6： | 2 |
| 3 | Modeling of the vehicle shimmy system with consideration of clearance of the steering linkage mechanism显示文摘 | Lu Jianwei Gu Jue Liu Mengjun | 2010 | Meccanica2010，45，3： | 1 |
| 4 | Identification of Avian-Derived Ingredients in Livestock and Poultry Meat by PCR Technology显示文摘In order to establish a quick and specific method which could identify the avian-derived ingredients,this study used 16 S rRNA gene sequence as target site,and designed the specific primers of chicken,pigeon meat and quail meat. The DNA of common livestock and poultry meat( including mutton,beef,pork,rabbit meat,pigeon meat,quail meat,chicken,duck and goose) was used as template. Though PCR amplification and specific detection,a quick determination method was established to identify the avian-derived ingredients. The results showed that the selected primers could identify the ingredients of animal origin effectively and quickly. The method was convenient and concise,and could detect the chicken-derived,pigeon-derived,quail-derived ingredients in livestock and poultry food quickly and accurately. | Jingxin ZHANG Yushi GAO Xiujun TANG Yanfeng FAN Mengjun TANG Dawei CHEN Qinglian GE Rong GU | 2016 | Asian Agricultural Research2016，8，12： | 0 |
| 5 | Establishment of an in vitro reporter system for screening HBx-targeting molecules显示文摘Chronic hepatitis B virus (HBV) infection remains a global public health problem. HBV-encoded X protein (HBx) is a multifunctional regulator that is required to initiate and maintain productive HBV infection, and is involved in HBV-related hepatocellular carci noma (HCC). Inhibitors that in terfere with the functions of HBx could be useful not only for the inhibition of HBV replication but also for the prevention or treatment of HBV-related HCC. To screen molecules that target HBx on a large scale remains a tech nical challenge due to a lack of sensitive and high-throughput system .In this work, we established an in vitro bioluminescent reporter system for screening HBx-targeting molecules. The system is based on a secretory fusion protein that combines HBx and NanoLuc (HBx-Nluc). The measured activity of NanoLuc in the culture supernatant of HBx-Nluc-expressing cells directly reflects the level of secreted HBx-Nluc. HBx protein-targeting intracellular anti-HBx single-chain variable fragment and RNA-targeting shRNA significantly reduced the secreted NanoLuc activity in HBx-Nluc-expressing cells. This system is simple and sensitive, and compatible with continuous non-disruptive screening, suggesting its potential usefulness for highthroughput screening and evaluating HBx-targeting molecules. | Chenjian Gu Shuai Tao Kongying Hu Lijun Ming Mengjun Luo Huimin Guo Yu Su Jing Liu Youhua Xie | 2019 | Acta Biochimica et Biophysica Sinica2019，51，4： | 0 |
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