|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Metal-Organic Frameworks-Based Fluorescent Nanocomposites for Bioimaging in Living Cells and in vivo显示文摘Metal-organic frameworks(MOFs)as a class of porous functional materials have attracted more and more attention for biomedical applications.To date,MOFs have been developed for bioimaging based on a series of advantages such as the large surface areas,high porosity,fluorescence functionalities and good biocompatibility.It is worth noting that organic or inorganic fluorescent materials such as fluorescent dyes,quantum dots,metal nanoclusters and nanosheets can combine MOFs or be encapsulated in MOFs to form fluorescent nanocomposites for excellent imaging function.Importantly,excellent imaging capabilities are of great significance in living cells and in vivo for detection,diagnosis and cancer therapy.In this review,we focus on the recent research of the bioimaging in living cells and in vivo based on various MOF-based nanocomposites and their potential biological clinical applications. | Meijia Liu Xiangling Ren Xianwei Meng Hongbo Li | 2021 | Chinese Journal of Chemistry2021，39，2： | 8 |
| 2 | Inactivation of LARS2, located at the commonly deleted region 3p21.3, by both epigenetic and genetic mechanisms in nasopharyngeal carcinoma显示文摘包括 3p21.3 区域,染色体 3p 的突变而产生之遗传的损失在 95100% 主要的鼻咽的癌(NPC ) 被发现活体检视,建议这个区域应该与 NPC 开发有关仔细怀有一些肿瘤 suppressor 基因(TSG ) 。在 3p21.3 定位的几 TSG 例如 RASSF1A, LTF 和 BLU,被表明了涉及 NPC 开发。LARS2 (leucyl-tRNA 合成酶 2, mitochondrial ) 另一基因位于染色体在 3p21.3 的 3 普通消除 region-1 (C3CER1 ) 。在这研究,我们在 NPC 集中了于 epigenetic 和 LARS2 的基因改变。LARS2 的 mRNA 表示被半量的反向的抄写聚合酶链反应(RTPCR ) 和即时 RTPCR 在 36 NPC 和 8 长期的鼻咽炎(NP ) 纸巾检测。随后, LARS2 的变化,突变而产生之遗传的损失,和 methylation 地位被聚合酶链 reaction-single-strand 符合构造多型性(PCR-SSCP ) 分析,同型结合的删除(HD ) 分析和 methylation 特定的聚合酶在主要 NPC 纸巾锁住反应。LARS2 的表示或下面规定都没在 78% 主要 NPC 纸巾被观察。没有变化,由定序的 PCR-SSCP 和 DNA 估计了,在倡导者区域和 exon 被发现在 NPC 纸巾的 1 LARS2,而 HD 在 LARS2 地点在 28% NPC 标本被检测。另外, LARS2 的 hypermethylation 在 64% NPC 样品被发现。我们的数据显示 LARS2 的那 inactivation 由基因并且 epigenetic 机制可以是在 NPC 的 carcinogenesis 的一个普通、重要的事件。 | Wen Zhou Xiangling Feng Hong Li Lei Wang Bin Zhu Weidong Liu Ming Zhao Kaitai Yao Caiping Ren | 2009 | Acta Biochimica et Biophysica Sinica2009，41，1： | 5 |
| 3 | Optical detection of acetylcholine esterase based on CdTe quantum dots显示文摘In this study, we have constructed a simple, sensitive and rapid biosensor for detection of acetylcholine esterase (AChE) based on CdTe quantum dots (QDs). The detection limit of AChE by one-step enzyme reaction based on the thiolglycolic acid (TGA) stabilized QDs (TGA-QDs) was 10 U/L and the linear range was 10-100 and 100-1200 U/L, respectively. The detection limit of AChE by two-step enzyme reaction based on the 3-mercaptopropionic acid (MGA) stabilized QDs (MGA-QDs) was found to be 20 U/L and the linear range was 100-2500 U/L. The experimental conditions of biosensors were optimized, and the detection mechanism was studied. We also detected AChE in serum samples based on TGA-QDs or MGA-QDs. The linear range was 10-140 and 50-1000 U/L, respectively. The excellent performance of this novel biosensor demonstrated that this strategy has prodigious potential to be applied in practice detection of AChE. | CHEN ZhenZhen REN XiangLing TANG FangQiong | 2013 | Chinese Science Bulletin2013，58，21： | 3 |
| 4 | Sensitive detection of dopamine and quinone drugs based on the quenching of the fluorescence of carbon dots显示文摘In this work,we demonstrated that the quinone structure can quench the fluorescence of the carbon dots(CDs).The sensitive determination of dopamine(DA) was studied primarily based on this principle.DA would be transformed into DA quinone under alkaline conditions,which resulted in fluorescence quenching of the CDs.A good linear range from 5 nmol/L to 0.4 mmol/L was obtained and the detection limit was 1 nmol/L.Moreover,the quenching effect of quinone structure on the fluorescence of CDs was confirmed by Fourier transform infrared spectra,time-correlated single-photon counting and X-ray photoelectron spectroscopy.Remarkably,CDs were firstly applied to detect the quinone drugs quantitatively which contained typical quinone structure based on the quenching mechanism.More than this,the sensing platform was demonstrated to provide credible selectivity and satisfactory stability in human serum solution with good liner range.Hence,our practical application and mechanism have showed great potential for diagnostic purposes. | Xiangling Ren Jiejie Ge Xianwei Meng Xiaozhong Qiu Jun Ren Fangqiong Tang | 2016 | Science Bulletin2016，61，20： | 3 |
| 5 | Corilagin inhibits SARS-CoV-2 replication by targeting viral RNA-dependent RNA polymerase显示文摘Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2) has become one major threat to human population health.The RNA-dependent RNA polymerase(RdRp) presents an ideal target of antivirals,whereas nucleoside analogs inhibitor is hindered by the proofreading activity of coronavirus.Herein,we report that corilagin(RAI-S-37) as a non-nucleoside inhibitor of SARS-CoV-2 RdRp,binds directly to RdRp,effectively inhibits the polymerase activity in both cell-free and cell-based assays,fully resists the proofreading activity and potently inhibits SARS-CoV-2 infection with a low 50% effective concentration(EC50) value of 0.13 μmol/L.Computation modeling predicts that RAI-S-37 lands at the palm domain of RdRp and prevents conformational changes required for nucleotide incorporation by RdRp.In addition,combination of RAI-S-37 with remdesivir exhibits additive activity against antiSARS-CoV-2 RdRp.Together with the current data available on the safety and pharmacokinetics of corilagin as a medicinal herbal agent,these results demonstrate the potential of being developed into one of the much-needed SARS-CoV-2 therapeutics. | Quanjie Li Dongrong Yi Xiaobo Lei Jianyuan Zhao Yongxin Zhang Xiangling Cui Xia Xiao Tao Jiao Xiaojing Dong Xuesen Zhao Hui Zeng Chen Liang Lili Ren Fei Guo Xiaoyu Li Jianwei Wang Shan Cen | 2021 | Acta Pharmaceutica Sinica B2021，11，6： | 2 |
| 6 | Superoxide dismutase mimetic ability of Mn-doped ZnS QDs显示文摘Superoxide dismutase(SOD) is an important antioxidant enzyme in the body. SOD has special physiological activity and is the primary substance for scavenging free radicals in living organisms.However, the expensive and complex extraction processes, low SOD yield, as well as difficult to store at room temperature have seriously hindered its application pace. Herein, the enzyme mimetic function of Mn-doped ZnS quantum dots(QDs) was discovered. The improved Marklund and McCord method both showed that Mn-doped ZnS QDs possess intrinsic SOD-like activity. The effects of temperature and pH on the mimetic enzyme activity of Mn-doped ZnS QDs have been investigated compared with SOD enzymes.The low cost and easy to synthesize white Mn-doped ZnS QDs with good biocompatibility are expected to be used as a new type of SOD nanozymes in the biology-relevant fields. | Xiangling Ren Mingqian Wang Xing He Zheng Li Jing Zhang Wei Zhang Xudong Chen Hong Ren Xianwei Meng | 2018 | Chinese Chemical Letters2018，29，12： | 2 |
| 7 | Construction and characterization of a full-length infectious clone of Getah virus in vivo显示文摘Getah virus(GETV)is a mosquito-borne virus of the genus Alphavirus in the family Togaviridae and,in recent years,it has caused several outbreaks in animals.The molecular basis for GETV pathogenicity is not well understood.Therefore,a reverse genetic system of GETV is needed to produce genetically modified viruses for the study of the viral replication and its pathogenic mechanism.Here,we generated a CMV-driven infectious cDNA clone based on a previously isolated GETV strain,GX201808(pGETV-GX).Transfection of pGETV-GX into BHK-21 cells resulted in the recovery of a recombinant virus(rGETV-GX)which showed similar growth characteristics to its parental virus.Then three-day-old mice were experimentally infected with either the parental or recombinant virus.The recombinant virus showed milder pathogenicity than the parental virus in the mice.Based on the established CMV-driven cDNA clone,subgenomic promoter and two restriction enzyme sites(BamHI and EcoRI)were introduced into the region between E1 protein and 3’UTR.Then the green fluorescent protein(GFP),red fluorescent protein(RFP)and improved light-oxygen-voltage(iLOV)genes were inserted into the restriction enzyme sites.Transfection of the constructs carrying the reporter genes into BHK-21 cells proved the rescue of the recombinant reporter viruses.Taken together,the establishment of a reverse genetic system for GETV provides a valuable tool for the study of the virus life cycle,and to aid the development of genetically engineered GETVs as vectors for foreign gene expression. | Tongwei Ren Xiangling Min Qingrong Mo Yuxu Wang Hao Wang Ying Chen Kang Ouyang Weijian Huang Zuzhang Wei | 2022 | Virologica Sinica2022，37，3： | 2 |
| 8 | 11-Mercaptoundecanoic acid functionalized gold nanoclusters as fluorescent probes for the sensitive detection of Cu^(2+) and Fe^(3+) ions显示文摘Metal ions are physiologically essential,but excessive metal ions may cause severe risk to plants and animals.Here,we prepared gold nanoclusters(Au NCs) protected by 11-mercaptoundecanoic acid(11-MUA),which have excellent fluorescence properties for the detection of metal ions.The results showed that the copper ions(Cu^(2+)) and iron ions(Fe^(3+)) in the solution have obvious quenching effect on the fluorescence intensity of Au NCs.The detection range of Fe^(3+) was 0.8–4.5 mmol/L(R^2= 0.992) and 4.5–11.0 mmol/L(R^2= 0.997).And Cu^(2+) has a lower linear range(0.1–1.0 mmol/L,R2= 0.993).When EDTA was added into the reaction system,it was observed that the quenching effect of Cu^(2+) and Fe^(3+)on Au NCs showed different phenomenon.Then,the effect of metal ions on the fluorescence of Au NCs was investigated.The selective detection of Cu^(2+) was achieved by EDTA masking of Fe^(3+).In addition,we realized the metal ions detection application of Au NCs in the | Zhiqun Bai Xiangling Ren Zhen Gong Chenxi Hao Yongmei Chen Pingyu Wan Xianwei Meng | 2017 | Chinese Chemical Letters2017，28，9： | 1 |
| 9 | Enhanced antibacterial activity of silica nanorattles with ZnO combination nanoparticles against methicillin-resistant Staphylococcus aureus显示文摘Silica nanorattles(SNs) with zinc oxide(ZnO) combination nanoparticles are reported to inhibit methicillin-resistant Staphylococcus aureus(MRSA) for the first time. SNs loaded with ZnO nanoparticles,which can produce free radicals, can cause severe damage to bacteria. ZnO nanoparticles not only provide free radicals in the combined nanostructures, which can inhibit the growth of bacteria, but also form nanorough surfaces with an irregular distribution of spikes on the SNs, which can enhance their adhesion to bacteria. Nanorough silica shell surfaces maintain the high activity and stability of small-sized ZnO nanoparticles and gather ZnO nanoparticles together to enhance production, which improves the efficiency of free radicals against the cytomembranes of bacterial cells. The enhanced adhesion of ZnO@SN nanoparticles to MRSA cells shortens the effective touching distance between free radicals and MRSA, which also improves antibacterial activity. As we expected, the ZnO@SN nanoparticles exhibit a better antibacterial effect than free ZnO nanoparticles against MRSA in vitro and in vivo. We also demonstrate that SNs loaded with ZnO nanoparticles can accelerate wound healing in MRSA skin inflammation models. This method of multilevel functionalization will be potentially applicable to the antibacterial field. | Qianqian Chai Qiong wu Tianlong Liu Longfei Tan Changhui Fu Xiangling Ren Yue Yang Xianwei Meng | 2017 | Science Bulletin2017，62，17： | 1 |
| 10 | A practical glucose biosensor based on Fe3O4 nanoparticles and chitosan/nafion composite film显示文摘 | Yang Liuqing Ren Xiangling Tang Fangqiong | 2009 | Biosensors and Bioelectronics2009，25，4： | 1 |
| 11 | Apractical glucose biosensor based on Fe3 04 nanoparticlesand chitosan/nafinon composite film显示文摘 | Yang Liuqing Ren Xiangling Tang Fangqiong | 2009 | Biosensors andBioelect2009，25，4： | 1 |
| 12 | Luminescent silver nanoclusters for efficient detection of adenosine triphosphate in a wide range of pH values显示文摘In this work,polymethacrylic acid(PMAA)-templated silver nanoclusters(Ag NCs)were developed as the fluorescent probe for the efficient and sensitive detection of adenosine triphosphate(ATP)in a wide range of pH values.The fluorescence intensity of the Ag NCs could keep stable with pH values ranging from2.5 to 9.3.The detection of ATP was based on the quenching of the fluorescent Ag NCs in the presence of ATP.The fluorescence quenching of the Ag NCs with increasing ATP concentration was studied at pH 2.5,4.5,7.0 and 8.5 which involved a wide pH environment in body fluids.The limit of detection(LOD)for ATP was as low as 0.1 mmol/L in an acidic environment with pH of 2.5 and all the linear correlation coefficients were satisfactory under wide-span pH values from 2.5 to 8.5.In addition,the sensitive determination of ATP was also achieved by adding copper ions(Cu^2+).The high selectivity and rapid detection process proved that the fluorescent probe had great potential to detect ATP in biological samples under different pH conditions. | Meijia Liu Xiangling Ren Xin Liu Longfei Tan Hui Li Jing Wei Changhui Fu Qiong Wu Jun Ren Hongbo Li Xianwei Meng | 2020 | Chinese Chemical Letters2020，31，12： | 1 |
| 13 | A practical glucose biosensor based on Fe3O4 nanoparticles and chitosan/nafion composite film显示文摘 | Yang Liuqing Ren Xiangling Tang Fangqiong | 2009 | Biosensors and Bioelectronics2009，25，4： | 1 |
| 14 | Using Silver Nanopartiele to Enhance Current Response of Biosen- sor显示文摘 | REN Xiangling MENG Xianwei CHEN Dong | 2004 | Biosens Bioelectron2004，21，3： | 1 |
| 15 | Using silver nanoparticle to enhance current response of biosensor显示文摘 | Ren Xiangling Meng Xianwei Chen Dong | 2005 | Biosensors and Bioelectronics2005，21，3： | 1 |
| 16 | A simpleand- sensitive fluorescence biosensor for detection of organophosphorus pesticides using H202-sensitive quantum dots/hi-enzyme 显示文摘 | Xianwei Meng Jianfei Wei Xiangling Ren et al | 2013 | Biosensors and Bioeleetronics2013，47，： | 1 |
| 17 | Fabrication of microwave-sensitized nanospheres of covalent organic framework with apatinib for tumor therapy显示文摘A new nanocomposite of hollow covalent organic framework(COF)conjugated with the apatinib(AP)and loading microwave-sensitizer(ionic liquid,IL)was prepared by layer by layer(LBL)method and hyaluronic acid(HA)coating,named as COF-AP-IL@HA.AP loading rate in COF hollow-spheres(~30 nm shell thickness)was~40.3%,due to the interactions of hydrogen andπ-πbonds between AP and COF shell,and acidic environment destroyed COF structure,promoting AP release.Microwave sensitization of loaded IL in COF hollow-spheres could enhance the microwave heat-effect,and combined AP therapeutic ability,leading to their higher inhibitation on tumor,due to targeting ability of HA and the local release of apatinib.88.9%of inhibition rate of COF-AP-IL@HA under microwave on the in vivo tumor was significantly higher than those without microwave(12.3%)and COF-IL@HA with microwave(37.5%),indicating a synergism of sensitized microwave hyperthermia and AP therapy on the reduced expression of VEGF via the downregulation pathway of hypoxia inducible factor.These results indicated that COF-AP-IL@HA was potential to the application in the combination therapy of tumor of the sensitized microwave hyperthermia and apatinib. | Qijun Du Jian Zou Zhongbing Huang Shimei Li Longfei Tan Xiangling Ren Guangfu Yin Yongfa Zheng Xianwei Meng | 2023 | Chinese Chemical Letters2023，34，5： | 0 |
| 18 | Nanoscale metal organic frameworks inhibition of pyruvate kinase of M2显示文摘Tumor cells usually show abnormally high glycolysis rate to maintain the dynamic balance of energy.The growth of tumor cells can be affected by inhibiting the activity of pyruvate kinase(especially M2-type isozyme,PKM2),the rate limiting enzyme of glycolysis.This is helpful to the treatment of tumor.Herein,metal organic frameworks(MOFs) were found to inhibit the activity of PKM2.Nanoscale ZIF-8 was synthesized by standing and ultrasonic method,respectively.The ZIF-8 has the performance of inhibiting PKM2.Further research showed that the inhibition ability was attributed to zinc ion in ZIF-8.Interestingly,the IC_(50) of ZIF-8 on PKM2 was one percent of that of zinc ion.This novel enzyme inhibitor is expected to be used in cancer therapy. | Xiangling Ren Xinyuan Huang Qiong Wu Longfei Tan Changhui Fu Yi Chen Xianwei Meng | 2021 | Chinese Chemical Letters2021，32，10： | 0 |
| 19 | Zirconium metal-organic framework nanocrystal as microwave sensitizer for enhancement of tumor therapy显示文摘Although surgical resection and chemotherapy were widely applied in tumor therapy, the dysfunction of normal cells resulted in the side effects (such as anorexia, nausea). MW thermal therapy is a non-invasive anticancer strategy under the help of MW sensitizer, with the safety and higher efficacy. Zirconium metal-organic framework nanocubes (ZrMOF NCs) modified with polyethylene glycol, were prepared via one pot method and carbodiimide technique, resulting in their large specific surface area and porosity.Our results showed that non-ion-loaded ZrMOF NCs in 0.9% NaCl solution exhibited better heating effect,higher than that in pure water, due to the robust collision among the ions under MW irradiation. The in vivo experiments confirmed that ZrMOF-PEG NCs + MW group exhibited the higher temperature in the tumor region than that of only MW treatment, suggesting a better MW thermal therapeutic anticancer efficacy. This work provides a new preparation strategy of biosafety nanomaterial as MW sensitizer for enhancing MW thermal anticancer therapy. | Yuanwen Zou Wei Zhang Hongqiao Zhou Changhui Fu Longfei Tan Zhongbing Huang Xiangling Ren Jun Ren Xudong Chen Xianwei Meng | 2019 | Chinese Chemical Letters2019，30，2： | 0 |
| 20 | 利用H3、H4组蛋白系统突变库解析生物组蛋白的功能显示文摘文章简介目前,组蛋白修饰酶的研究已表明,组蛋白的表观修饰与生长、发育、癌症等存在密切联系。但由于非组蛋白底物的存在和组蛋白多位点修饰,对组蛋白本身某一修饰功能的理解仍极具挑战,且组蛋白基因在多细胞真核生物中呈现多拷贝和多分布等复杂特征,这极大地阻碍了以组蛋白为核心的表观遗传学发展。 | Weimin Zhang Xuedi Zhang Zhaoyu Xue Yijie Li Qing Ma Xiangle Ren Jiaying Zhang Songhua Yang Lijuan Yang Menghua Wu Mengda Ren Rongwen Xi Zheng Wu Ji-Long Liu Erika Matunis 戴俊彪 高冠军 | 2020 | 科学新闻2020，，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员