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| 1 | H7N9 virulent mutants detected in chickens in China pose an increased threat to humans显示文摘 | Jianzhong Shi Guohua Deng Huihui Kong Chunyang Gu Shujie Ma Xin Yin Xianying Zeng Pengfei Cui Yan Chen Huanliang Yang Xiaopeng Wan Xiurong Wang Liling Liu Pucheng Chen Yongping Jiang Jinxiong Liu Yuntao Guan Yasuo Suzuki Mei Li Zhiyuan Qu Lizheng Guan Jinkai Zang Wenli Gu Shuyu Han Yangming Song Yuzhen Hu Zeng Wang Linlin Gu Wenyu Yang Libin Liang Hongmei Bao Guobin Tian Yanbing Li Chuanling Qiao Li Jiang Chengjun Li Zhigao Bu Hualan Chen | 2017 | Cell Research2017，27，12： | 70 |
| 2 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 3 | Injectable hydrogels for cartilage and bone tissue engineering显示文摘Tissue engineering has become a promising strategy for repairing damaged cartilage and bone tissue. Among the scaffolds for tissue-engineering applications, injectable hydrogels have demonstrated great potential for use as three-dimensional cell culture scaffolds in cartilage and bone tissue engineering, owing to their high water content, similarity to the natural extracellular matrix(ECM), porous framework for cell transplantation and proliferation, minimal invasive properties, and ability to match irregular defects. In this review, we describe the selection of appropriate biomaterials and fabrication methods to prepare novel injectable hydrogels for cartilage and bone tissue engineering. In addition, the biology of cartilage and the bony ECM is also summarized. Finally, future perspectives for injectable hydrogels in cartilage and bone tissue engineering are discussed. | Mei Liu Xin Zeng Chao Ma Huan Yi Zeeshan Ali Xianbo Mou Song Li Yan Deng Nongyue He | 2017 | Bone Research2017，5，2： | 54 |
| 4 | Mortality and Morbidity of Extremely Low Birth Weight Infants in the Mainland of China: A Multi-center Study显示文摘 | Hui-Jia Lin Li-Zhong Du Xiao-Lu Ma Li-Ping Shi Jia-Hua Pan Xiao-Mei Tong Qiu-Ping Li Jian-Guo Zhou Bing Yi Ling Liu Yun-Bing Chen Qiu-Fen Wei Hui-Qing Wu Mei Li Cui-Qing Li Xi-Rong Gao Shi-Wen Xia Wen-Bin Li Chao-Ying Ya Ling He Kun Liang Xiao-Yu Zhou Shu-Ping Han Qin Lyu Yin-Ping Qiu Wen Li Dong-Mei Chen Hong-Ru Lu Xiao-Hong Liu Hong Liu Zhen-Lang Lin Li Liu Jia-Jun Zhu Hong Xiong Shao-Jie Yue Si-Qi Zhuang | 2015 | Chinese Medical Journal2015，，20： | 49 |
| 5 | 中国脑卒中及其危险因素的流行特征变化趋势(2002—2013年)显示文摘随着人均期望寿命的增加和人口老龄化的加剧,脑卒中已成为中国成年人首要的致死和致残疾病;同时,脑卒中及其危险因素的流行特征可能也发生了巨大的变化。然而,自20世纪90年代以后,尚没有覆盖全国范围的、基于社区人群的脑卒中流行病学调查。中国脑卒中筛查项目(China National Stroke Screening Survey,CNSSS)是一项覆盖全国31个省、市、自治区,基于社区人群的脑卒中筛查项目,旨在监测中国人群脑卒中及其危险因素的变化趋势,并评价相应的脑卒中干预策略和措施。 | Tianjia Guan Jing Ma Mei Li 郭健(编译) | 2019 | 中华预防医学杂志2019，53，2： | 39 |
| 6 | Assessment of source water contamination by estrogenic disrupting compounds in China显示文摘Detection of estrogenic disrupting compounds(EDCs) in drinking waters around China has led to rising concerns about health risks associated with these compounds.There is,however,a paucity of studies on the occurrence and identification of the main compounds responsible for this pollution in the source waters.To fill this void,we screened estrogenic activities of 23 source water samples from six main river systems in China,using a recombinant two-hybrid yeast assay.All sample extracts induced significant estrogenic activity,with E2 equivalents(EEQ) of raw water ranging from 0.08 to 2.40 ng/L.Additionally,16 samples were selected for chemical analysis by gas chromatography-mass spectrometry.The EDCs of most concern,including estrone(E1),17β-estradiol(E2),17α-ethinylestradiol(EE2),estriol(E3),diethylstilbestrol(DES),estradiol valerate(EV),4-t-octylphenol(4-t-OP),4-nonylphenols(4-NP) and bisphenol A(BPA),were determined at concentrations of up to 2.98,1.07,2.67,4.37,2.52,1.96,89.52,280.19 and 710.65 ng/L,respectively.Causality analysis,involving comparison of EEQ values from yeast assay and chemical analysis identified E2,EE2 and 4-NP as the main responsible compounds,accounting for the whole estrogenic activities(39.74% to 96.68%).The proposed approach using both chemical analysis and yeast assay could be used for the identification and evaluation of EDCs in source waters of China. | Weiwei Jiang YeYan Mei Ma Donghong Wang Qian Luo Zijian Wang Senthil Kumaran Satyanarayanan | 2012 | Journal of Environmental Sciences2012，24，2： | 29 |
| 7 | Herbal decoctosome is a novel form of medicine显示文摘Traditionally, herbal medicine is consumed by drinking decoctions produced by boiling herbs with water. The functional components of the decoction are heat stable. Small RNAs(sRNAs) were reported as a new class of functional components in decoctions. However, the mechanisms by which sRNAs survive heat treatment of the decoction and enter cells are unclear.Previous studies showed that plant-derived exosome-like nanoparticles(ELNs), which we call botanosomes, could deliver therapeutic reagents in vivo. Here, we report that heat-stable decoctosomes(ELNs) from decoctions have more therapeutic effects than the decoctions in vitro and demonstrate therapeutic efficacy in vivo. Furthermore, sRNAs, such as HJT-sRNA-m7 and PGY-sRNA-6, in the decoctosome exhibit potent anti-fibrosis and anti-inflammatory effects, respectively. Decoctosome is comprised of lipids, chemical compounds, proteins, and s RNAs. A medical decoctosome mimic is called bencaosome. A single lipid sphinganine(d22:0) identified in the decoctosome was mixed and heated with the synthesized sRNAs to form the simplest bencaosome. This simple bencaosome structure was identified by critical micelle concentration(cmc) assay that sRNAs coassembled with sphinganine(d22:0) to form the lipid layers of vesicles. The heating process facilitates co-assembly of sRNAs and sphinganine(d22:0) until a steady state is reached. The artificially produced sphinganine-HJT-sRNA-m7 and sphinganinePGY-sRNA-6 bencaosomes could ameliorate bleomycin-induced lung fibrosis and poly(I:C)-induced lung inflammation, respectively, following oral administration in mice. Our study not only demonstrates that the herbal decoctosome may represent a combinatory remedy in precision medicine but also provides an effective oral delivery route for nucleic acid therapy. | Xiaoyun Li Zhu Liang Jianchao Du Zhiqing Wang Song Mei Zhiqing Li Yan Zhao DANDan Zhao Yiming Ma Jun Ye Jiantao Xu Yu Zhao Jiahui Chang Yuhao Qin Lanlan Yu Chenxuan Wang Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 26 |
| 8 | Apoptosis-inducing activity of the antimicrobial peptide cecropin of Musca domestica in human hepatocellular carcinoma cell line BEL-7402 and the possible mechanism显示文摘我们在人的 hepatocellular 癌房间线 BEL-7402 和它的内在的机制学习了蝇属 domestica 的抗菌剂肽 cecropin 的导致 apoptosis 性质。人的 hepatocellular 癌 BEL-7402 房间和人的正常的肝房间的增长抑制被 MTT 试金决定,并且房间生存能力被 trypan 蓝色染料排除试金决定。与 cecropin 对待的 apoptotic 肿瘤房间被传播电子显微镜学和 terminal-deoxynucleotidyl transferase 检验标记的调停的刻痕结束。apoptosis 率被流动 cytometry (FCM ) 与 PI/Annexin-V 测量两倍染色。西方的污点分析和 RT-PCR 被用来决定涉及 apoptosis 的蛋白质的表示层次,例如船边交货, Fas-L, caspase-8,和 caspase-3。试验性的结果证明蝇属 domestica cecropin 在剂量依赖者和时间依赖者礼貌禁止了人的 hepatocellular 癌 BEL-7402 房间的增长,没有影响正常的肝房间的增长。FCM 证明房间 apoptosis 率是 5. | Xiaobao Jin | 2010 | Acta Biochimica et Biophysica Sinica2010，42，4： | 23 |
| 9 | The Chinese Society of Clinical Oncology(CSCO)clinical guidelines for the diagnosis and treatment of nasopharyngeal carcinoma显示文摘Nasopharyngeal carcinoma(NPC)is a malignant epithelial tumor originating in the nasopharynx and has a high incidence in Southeast Asia and North Africa.To develop these comprehensive guidelines for the diagnosis and management of NPC,the Chinese Society of Clinical Oncology(CSCO)arranged a multi-disciplinary team comprising of experts from all sub-specialties of NPC to write,discuss,and revise the guidelines.Based on the findings of evidencebased medicine in China and abroad,domestic experts have iteratively developed these guidelines to provide proper management of NPC.Overall,the guidelines describe the screening,clinical and pathological diagnosis,staging and risk assessment,therapies,and follow-up of NPC,which aim to improve the management of NPC. | Ling-Long Tang Yu-Pei Chen Chuan-Ben Chen Ming-Yuan Chen Nian-Yong Chen Xiao-Zhong Chen Xiao-Jing Du Wen-Feng Fang Mei Feng Jin Gao Fei Han Xia He Chao-Su Hu De-sheng Hu Guang-Yuan Hu Hao Jiang Wei Jiang Feng Jin Jin-Yi Lang Jin-Gao Li Shao-Jun Lin Xu Liu Qiu-Fang Liu Lin Ma Hai-Qiang Mai Ji-Yong Qin Liang-Fang Shen Ying Sun Pei-Guo Wang Ren-Sheng Wang Ruo-Zheng Wang Xiao-Shen Wang Ying Wang Hui Wu Yun-Fei Xia Shao-Wen Xiao Kun-Yu Yang Jun-Lin Yi Xiao-Dong Zhu Jun Ma | 2021 | Cancer Communications2021，41，11： | 22 |
| 10 | Crystal structure and biochemical analyses reveal Beclin 1 as a novel membrane binding protein显示文摘Beclin 1 基因是 haplo 不够的肿瘤 suppressor 并且在 autophagy 起一个必要作用。然而, Beclin 1 由工作的分子的机制仍然保持大部分未知。这里,我们在 1.6 Å 报导 Beclin 1 的 evolutionarily 保存的领域(ECD ) 的水晶结构;分辨率。Beclin 1 ECD 展览一以前 unreported 褶层,与在中央轴附近相称性地安排的三次结构的重复。Beclin 1 ECD 定义膜绑定领域的一个新奇的类,与为与 cardiolipin 充实的类脂化合物膜的强壮的偏爱。在 Beclin 1 ECD 的一个表面环的尖端,包括三芳香的氨基酸,与类脂化合物膜充当一根恐水病的手指到伙伴,因而导致膜和 liposomes 的变丑。这些芳香的残余的变化使 Beclin 1 变为不能稳定地在 vitro 与类脂化合物膜联系并且不能充分在 vivo 在 Beclin 1-knockdown 房间救 autophagy。这些观察为译解形成一个重要框架 Beclin 1 的生物功能。 | Weljlao Huang Wooyoung Chol Wanqiu Hu Na Mi Qiang Guo Meisheng Ma Mei Liu Yuan Tian Peilong Lu Feng-Liang Wang Haiteng Deng Lei Liu Ning Gao Li Yu Yigong Shi | 2012 | Cell Research2012，22，3： | 21 |
| 11 | Basic Lithofacies-Succession Model for the Wumishan Cyclothems: Their Markov Chain Analysis and Regularly Vertical Stacking Patterns in the Third-Order Sequences显示文摘The Mesoproterozoic Wumishan Formation in the Jixian section ofTianjin is a succession of 3300-m-thick carbonate strata formed in a period of about 100 Ma (1310 ± 20 Ma-1207 ± 10 Ma). In this succession of strata, the carbonate metre-scale cyclic sequences belonging to peritidal type with an approximately symmetrical lithofacies succession are best developed. The wide development of 1:4 stacking patterns shows that these metre-scale cyclic se quences are genetically related to the short-eccentricity cycles, which are called the Wumishan cyclothems that could truly represent sedimentary cycles. Generally, massive and thick-bedded calcareous dolomites and dolomitic limestones of stromatolite biostromes and thrombolite bioherms constitute the central part of the Wumishan cyclothems. The lower and upper parts consist of tidal flat dolostones, sandy-muddy dolostone and the top part is composed of lagoonal facies dolo mitic shales with a paleosol cap. Therefore, an approximately symmetrical lithofacies-succession is formed. Many features such as the clear deepening and shoaling vectors of cyclothems, and all kinds of marks of fresh-water diagenesis indicate that the Wumishan cyclothems are the product of autocyclic sedimentation governed by allocyclic high-frequency sea level changes. The results of a Markov chain analysis reaffirm the existence of the lithofacies-succession model of the Wumishan cyclothems. The boundaries of the Wumishan cyclothems are marked by the instantaneous exposed punctuated surface, which leads to the discrepancy between the cyclothems and the parasequences of the sequence stratigraphy ter minology system. It is difficult to form a judgment that the time span of the Milankovitch cycles in the Precambrian is certainly equal to that of the Phanerozoic, but the regularly vertical stacking patterns of the seventh-order rhythms, sixth order cyclothems and fifth-order parasequence sets still indicate their consistency with the duration of the Milankovitch cycles in the Phanerozoic. | MEI Mingxiang, MA Yongsheng and GUO Qingyin Institute of Earth Sciences and Natural Resources, China University of Geosciences, Beijing 100083 China Petrochemical Corporation (SINOPEC), Beijing 100029 | 2001 | Acta Geologica Sinica(English Edition)2001，75，4： | 19 |
| 12 | Metal chlorides loaded on activated carbon to capture elemental mercury显示文摘Activated carbon (AC) was considered to be an effective sorbent to control mercury in combustion systems. However, its capture capacity was low and it required a high carbon-to-mercury mass ratio. AC loaded with catalyst showed a high elemental mercury (Hg 0 ) capture capacity due to large surface area of AC and high oxidization ability of catalyst. In this study, several metal chlorides and metal oxides were used to promote the sorption capacity of AC. As a result, metal chlorides were better than metal oxides loaded on AC to remove gaseous mercury. X-ray diffractometer (XRD), thermogravimetric analyzer (TGA) and specific surface area by Brunauer- Emmett-Teller method (BET) analysis showed the main mechanisms: first, AC had an enormous surface area for loading enough MClx; second, Cl and MxOy were generated during pyrogenation of MClx; finally, there were lots of active elements such as Cl and MxOy which could react with elemental mercury and convert it to mercury oxide and mercury chloride. The HgO and HgCl 2 might be released from AC's porous structure by thermo regeneration. A catalytic chemisorption mechanism predominates the sorption process of elemental mercury. As Co and Mn were valence variable metal elements, their catalytic effect on Hg 0 oxidization may accelerate both oxidation and halogenation of Hg 0 . The sorbents loaded with metal chlorides possessed a synergistic function of catalytic effect of valence variable metal and chlorine oxidation. | Zhemin Shen,Jing Ma,Zhijian Mei,Jianda Zhang School of Environmental Science and Engineering,Shanghai Jiao Tong University,Shanghai 200240,China | 2010 | Journal of Environmental Sciences2010，22，11： | 18 |
| 13 | Interleukin-22 ameliorates liver fibrogenesis by attenuating hepatic stellate cell activation and downregulating the levels of inflammatory cytokines显示文摘AIM:To investigate the effect of interleukin(IL)-22 onhepatic fibrosis in mice and the possible mechanism involved.METHODS:Liver fibrosis was induced in male BALB/c mice by CCl4.Recombinant IL-22(rm IL-22) was administered intraperitoneally in CCl4-treated mice.Fibrosis was assessed by histology and Masson staining.The activation of hepatic stellate cells(HSCs) was investigated by analysis of α-smooth muscle actin expression.The frequencies of T helper(Th) 22 cells,Th17 cells and Th1 cells,the expression of inflammatory cytokines [IL-22,IL-17 A,interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α),IL-6,IL-1b] and transcription factors [aryl hydrocarbon receptor(AHR),RAR-related orphan receptor(RORγt),T-bet] m RNA in the liver were investigated.In addition,the plasma levels of IL-22,IL-17 A,IFN-γ,TNF-α,IL-6 and IL-1b were evaluated.RESULTS:Significant elevations in circulating Th22 cells,Th17 cells,Th1 cells,IL-22,IL-17 A,and IFN-γ were observed in the hepatic fibrosis group compared with the control group(P < 0.01).Treatment with rm IL-22 in mice with hepatic fibrosis ameliorated the severity of hepatic fibrosis,which was confirmed by lower hepatic fibrosis pathological scores(P < 0.01).Rm IL-22 decreased the frequencies of Th22 cells(6.71% ± 0.97% vs 8.09% ± 0.74%,P < 0.01),Th17 cells(4.34% ± 0.37% vs 5.71% ± 0.24%,P < 0.01),Th1 cells(3.09% ± 0.49% vs 4.91% ± 0.73%,P < 0.01),and the levels of IL-22(56.23 ± 3.08 vs 70.29 ± 3.01,P < 0.01),IL-17A(30.74 ± 2.77 vs 45.68 ± 2.71,P < 0.01),and IFN-γ(74.78 ± 2.61 vs 124.89 ± 2.82,P < 0.01).Down-regulation of IL-22,IL-17 A,IFN-γ,TNF-α,IL-6,IL-1b,AHR RORγt,and T-bet gene expression in the liver was observed in the rm IL-22 group(P < 0.01).CONCLUSION:The frequencies of Th22,Th17 andTh1 cells are elevated in hepatic fibrosis.Rm IL-22 can attenuate HSC activation and down-regulate the levels of inflammatory cytokines,thereby ameliorating liver fibrogenesis. | Dong-Hong Lu Xiao-Yun Guo Shan-Yu Qin Wei Luo Xiao-Li Huang Mei Chen Jia-Xu Wang Shi-Jia Ma Xian-Wen Yang Hai-Xing Jiang | 2015 | World Journal of Gastroenterology2015，21，5： | 18 |
| 14 | Treatment and outcomes of 1041 pediatric patients with neuroblastoma who received multidisciplinary care in China显示文摘Importance:Neuroblastoma is the most common extracranial malignant solid tumor in children.Multidisciplinary care is critical to improving the survival of pediatric patients with neuroblastoma.Objective:To systematically summarize the clinical characteristics of children with neuroblastoma and evaluate their prognosis with multidisciplinary care provided in a single center.Methods:This retrospective study analyzed the clinical data of 1041 patients with neuroblastoma who were diagnosed,treated,and followed-up in the Hematology-Oncology Center of Beijing Children’s Hospital from 2007 to 2019.Results:The median age at diagnosis was 34 months;80.8%of the patients were younger than 5 years of age.Notably,243 patients(23.3%)were classified as low-risk,249 patients(23.9%)were classified as intermediate-risk,and 549(52.7%)were classified as high-risk.Furthermore,956 patients underwent surgical resections;986(94.7%)patients received chemotherapy;and 176 patients with high-risk neuroblastoma received hematopoietic stem cell transplantation.The 5-year event-free survival(EFS)rate was 91.3%and 5-year overall survival(OS)rate was 97.5%in low-risk group;in the intermediate-risk group,these rates were 85.1%and 96.7%,respectively,while they were 37.7%and 48.9%in the high-risk group(P<0.001 for both).The 5-year EFS and OS rates were significantly higher in patients diagnosed between 2015 and 2019 than in patients diagnosed between 2007 and 2014(P<0.001).In total,278 patients(26.7%)exhibited tumor relapse or progression;the median interval until relapse or progression was 14 months.Of the 233 patients who died,83%died of relapse or progression of neuroblastoma and 4.3%died of therapy-related complications.Interpretation:The 5-year OS rate was low in high-risk patients,compared with low-and intermediate-risk patients.Multidisciplinary care is critical for improvement of survival in pediatric patients with neuroblastoma.Additional treatment strategies should be sought to improve the prognosis of patients with high-risk neuroblastoma. | Yan Su Hong Qin Chenghao Chen Shengcai Wang Shihan Zhang Dawei Zhang Mei Jin Yun Peng Lejian He Xiaoman Wang Suyun Qian Maoquan Qin Ming Ge Fuquan Zhang Qi Zeng Huanmin Wang Xiaoli Ma Xin Ni | 2020 | Pediatric Investigation2020，4，3： | 16 |
| 15 | Clinical value of preoperative methylated septin 9 in Chinese colorectal cancer patients显示文摘BACKGROUND The methylated septin 9(mSEPT9) assay was the first blood-based test approved by the United States Food and Drug Administration as a colorectal screening test.However, the diagnostic and prognostic role of preoperative mSEPT9 for colorectal cancer(CRC) in Chinese patients is still unknown.AIM To improve the understanding of diagnostic and prognostic factors, serum m SEPT9 was detected in Chinese CRC patients.METHODS A retrospective analysis of 354 cases, of which 300 had CRC and 54 were normal,was performed in China. Patients' characteristics, treatments, and laboratory data, including age, the date of surgery, Union for International Cancer Control(UICC) stages, distant metastasis(M), and so on, were collected. Methylation levels of SEPT9 were quantified by quantitative, methylation-specific polymerase chain reaction before surgery. In addition, the effects of mSEPT9 on the occurrence and prognosis of 330 CRC cases from The Cancer Genome Atlas(TCGA) database were evaluated using bioinformatics analyses. Potential prognostic factors for overall survival(OS) and progression-free survival(PFS)were evaluated by Kaplan-Meier univariate analysis.RESULTSIn Chinese CRC patients, positive mSEPT9 was strongly associated with advanced UICC stages, deeper invasion by the primary tumor, and more distant metastasis. Methylation levels of SEPT9 were stage-dependent and showed a stepwise increase in UICC stages(I–IV), primary tumor categories(T1–T4),regional node categories(N0–N2), and distant metastasis categories(M0–M1).The patients with positive mSEPT9 showed a tendency toward lower PFS. After analyzing TCGA clinical data, the high mSEPT9 group was found to be obviously correlated only with more distant metastasis. The patients with high mSEPT9 levels showed a tendency toward lower OS. Besides, nine meaningful mSEPT9 sites were found to provide guidance for the follow-up studies.CONCLUSION MSEPT9 analysis may add valuable information to current tumor staging. Serum m SEPT9 in Chinese CRC patients appears to offer promising novel prognostic markers and might be considered for monitoring CRC recurrence. | Xue Yang Zhi-Jie Xu Xi Chen Shuang-Shuang Zeng Long Qian Jie Wei Mei Peng Xiang Wang Wan-Li Liu Hong-Ying Ma Zhi-Cheng Gong Yuan-Liang Yan | 2019 | World Journal of Gastroenterology2019，25，17： | 16 |
| 16 | Brucine Suppresses Breast Cancer Metastasis via Inhibiting Epithelial Mesenchymal Transition and Matrix Metalloproteinases Expressions显示文摘Objective: To examine the effect of brucine on the migration, invasion, adhesion and expressions of epithelial-to-mesenchymal transition(EMT) markers and matrix metalloproteinases(MMPs) in the highly metastatic breast cancer cell lines MDA-MB-231 and Hs578-T. Methods: MDA-MB-231 and Hs578-T cells were divided to 4 groups: the control group(0.1% DMSO), and 25, 50 and 100 μmol/L brucine groups. The cell viability was determined using a Cell Titer-Glo? luminescent cell viability. The scratch wound healing assay and tanswell migration assay were used to determine the migration ability of these cells treated by different concentrations of brucine. The proliferation rate, invasive potential and adhesive ability were respectively performed by colony formation assay, transwell invasion assay and adhension assay. The protein and m RNA expressions of EMT biomarkers, MMP-2 and MMP-9 were investigated by real-time reverse transcription polymerase chain reaction and Western blot. Results: Compared with the control group, brucine had little effect on cell viability or proliferation(P>0.05), but led to a dose-dependent decrease on migration, invasion, adhension of MDA-MB-231 and Hs578-T cells(P<0.01). Furthermore, brucine increased the protein and m RNA levels of EMT markers such as E-cadherin and β-catenin in MDA-MB-231 and Hs578-T cells, and decreased the protein and m RNA levels of mesenychmal markers such as vimentin and fibronectin, as well as the expressions of MMP-2 and MMP-9(all P<0.01). Conclusion: Brucine inhibited triple negative breast cancer cells metastasis potentially through EMT reversion and MMP-2 and MMP-9 inhibition. | LI Miao LI Ping ZHANG Mei MA Feng | 2018 | Chinese Journal of Integrative Medicine2018，24，1： | 15 |
| 17 | Nasopharyngeal carcinoma:Advances in genomics and molecular genetics显示文摘Nasopharyngeal carcinoma (NPC) is a squamous-cell carcinoma that arises in the epithelial lining of the nasopharynx [1]. This neoplasm has a notable ethnic and geographic distribution,being of high prevalence in southern China but rare in other parts of the world [2]. | ZENG ZhaoYang HUANG HongBin ZHANG WenLing XIANC Bo ZHOU Ming ZHOU YanHong MA Jian YI Mei LI XiaYu LI XiaoLing XIONG Wei LI GuiYuan | 2011 | Science China(Life Sciences)2011，54，10： | 13 |
| 18 | Genotypic Characterization of Methicillin-resistant Staphylococcus aureus Isolated from Pigs and Retail Foods in China显示文摘Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus(MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. Methods Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing(MLST), spa typing, multiple-locus variable number of tandem repeat analysis(MLVA), pulsed-field gel electrophoresis(PFGE), and antimicrobial susceptibility testing. Results All isolated MRSA exhibited multi-drug resistance(MDR). Greater diversity was found in food-associated MRSA(7 STs, 8 spa types, and 10 MLVA patterns) compared to pig-associated MRSA(3 STs, 1 spa type, and 6 MLVA patterns). PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates(40 vs. 11 pulse types). Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone(96.4%), and CC9-ST9-t437-MC621(20.0%) was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig-and food-associated isolates. Of note, some community-and hospital-associated MRSA strains(t030, t172, t1244, and t4549) were also identified as food-associated isolates. Conclusion CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China. | WANG Wei LIU Feng ZULQARNAIN Baloch ZHANG Cun Shan MA Ke PENG Zi Xin YAN Shao Fei HU Yu Jie GAN Xin DONG Yin Ping BAI Yao LI Feng Qin YAN Xiao Mei MA Ai Guo XU Jin | 2017 | Biomedical and Environmental Sciences2017，30，8： | 13 |
| 19 | The influence of temperature on rare earth flotation with naphthyl hydroxamic acid显示文摘The influence of temperature on the complex process of Bayan Obo rare earth(RE) ore flotation with a collector of naphthyl hydroxamic acid(LF8#) was investigated. Industrial test data shows that the grade and recovery of RE increase with the temperature. However, the proportion of bastnaesite in the bulk concentrate increases as the RE grade improves. Adsorption mechanism of LF8# on the surfaces of bastnaesite and monazite were confirmed via zeta potential, UV/Vis Spectrophotometer(UV/Vis), Fourier transform infrared(FTIR), and X-ray photoelectron spectroscopy analyses(XPS). Although the results indicate that the total amount of the LF8# adsorption on the surface of bastnaesite and monazite decreases with increasing the temperature, the amount of stable adsorbed predominance of characteristic bonds(-C(=O)N-) from LF8# uptake on bastnaesite surfaces increases significantly at high temperatures. This conclusion indicates that the adsorption stability increases with increasing the temperature.For monazite, the amount of characteristic elements C and N in LF8# does not increase as the temperature increases on the mineral surface, but the proportion of characteristic bonds increases, which shows that the adsorption stability of LF8# on the surface of monazite also increases, but it is not as significant as bastnaesite, which may be one of the reasons that the floatability of bastnaesite is better than those of monazite. Pulp dispersion results show that the temperature improve the dispersions of both the gangue and RE minerals. This improved the flotation selectivity so that it favored RE minerals. The calculated bubble size distribution confirms that higher temperatures generate smaller bubbles, thereby increasing the bubble-particle collision probability and the recovery of RE minerals. | Mei Li Kai Gao Dongliang Zhang Haijun Duan Linlin Ma Lan Huang | 2018 | Journal of Rare Earths2018，36，1： | 13 |
| 20 | Comparative analyses of leaf anatomy of dicotyledonous species in Tibetan and Inner Mongolian grasslands显示文摘Knowledge of the leaf anatomy of grassland plants is crucial for understanding how these plants adapt to the environment. Tibetan alpine grasslands and Inner Mongolian temperate grasslands are two major grassland types in northern China. Tibetan alpine grasslands occur in high-altitude regions where the low temperatures limit plant growth. Inner Mongolian temperate grasslands are found in arid regions where moisture is the limiting factor. Few comparative studies concerning the leaf anatomy of grassland plants of the Tibetan Plateau and Inner Mongolian Plateau have been conducted. We examined leaf characteristics at 71 sites and among 65 species, across the alpine grasslands of the Tibetan Plateau and the temperate grasslands of the Inner Mongolian Plateau. We compared the leaf structures of plants with different life forms and taxonomies, and their adaptation to arid or cold environments. We explored relationships among leaf features and the effects of climatic factors (i.e., growing season temperature and precipitation) on leaf characteristics. Our results showed that (i) there were significant differences in leaf anatomy between Tibetan alpine and Inner Mongolian temperate grasslands. Except for mesophyll cell density, the values obtained for thickness of leaf tissue, surface area and volume of mesophyll cells were larger on the Tibetan Plateau than on the Inner Mongolian Plateau. (ii) Within the same family or genus, leaf anatomy showed significant differences between two regions, and trends were consistent with those of whole species. (iii) Leaf anatomy of woody and herbaceous plants also showed significant differences between the regions. Except for mesophyll cell density, the values obtained for the thickness of leaf tissue, and the surface area and volume of mesophyll cells were larger in herbaceous than in woody plants. (iv) Leaf anatomical traits changed accordingly. Total leaf thickness, thicknesses of lower and upper epidermal cells, and surface area and volume of mesophyll cells were positively correlated, while mesophyll cell density was negatively associated with those traits. (v) Growing season temperature had stronger effects on leaf anatomy than growing season precipitation. Although the communities in Tibetan and Inner Mongolian grasslands were similar in appearance, leaf anatomy differed; this was probably due to the combined effects of evolutionary adaptation of plants to environment and environmental stress induced by climatic factors. | MA JianJing JI ChengJun HAN Mei ZHANG TingFang YAN XueDong HU Dong ZENG Hui HE JinSheng | 2012 | Science China(Life Sciences)2012，55，1： | 12 |
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