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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | TLR2 and TLR4 polymorphisms influence m RNA and protein expression in colorectal cancer显示文摘AIM: To evaluate the effect of promoter region polymorphisms of toll-like receptor(TLR)2-196 to-174 del and TLR4-1607T/C(rs10759932) on m RNA and protein expression in tumor tissue and of TLR4+896A/G(rs4986790) on colorectal cancer(CRC) risk.METHODS: The TLR2-196 to-174 del polymorphism was investigated using allele-specific polymerase chain reaction(PCR) and the TLR4-1607T/C and TLR4+896A/G by PCR-restriction fragment length p o l y m o r p h i s m( R F L P). W e g e n o t y p e d 4 3 4 D N A samples from 194 CRC patients and 240 healthy individuals. The m RNA relative quantification(RQ) was performed in 40 tumor tissue samples by quantitative PCR Taq Man assay, using specific probes for TLR2 and TLR4 genes, and ACTB and GAPDH reference geneswere used as endogenous controls. Protein expression was analyzed by immunohistochemistry with specific primary antibodies.RESULTS: No association was found for TLR4-1607T/C and TLR4+896A/G by three statistical models(logadditive, dominant and recessive). However, based on dominant and log-additive models, the polymorphic variant TLR2-196 to-174 del was associated with increased CRC risk [dominant: odds ratio(OR) = 1.72, 95%CI: 1.03-2.89; P = 0.038 and log-additive: OR =1.59, 95%CI: 1.02-2.48; P = 0.039]. TLR2 m RNA expression was increased in tumor tissue(RQ = 2.36) when compared to adjacent normal tissue(RQ = 1; P < 0.0001), whereas the TLR4 m RNA showed a basal expression(RQ = 0.74 vs RQ = 1, P = 0.452). Immunohistochemistry analysis of TLR2 and TLR4 protein expression was concordant with the findings of m RNA expression. In addition, the TLR2-196 to-174 del variant carriers showed m RNA relative expression 2.19 times higher than wild-genotype carriers. The TLR2 protein expression was also higher for the TLR2-196 to-174 del variant carriers [117 ± 10 arbitrary unit(a.u.) vs 95 ± 4 a.u., P = 0.03]. However, for the TLR4-1607T/C polymorphism no significant difference was found for both m RNA(P = 0.56) and protein expression(P = 0.26).CONCLUSION: Our findings suggest that TLR2-196 to-174 del polymorphism increases TLR2 m RNA expression and is associated with higher CRC risk, indicating an important role in CRC genetic susceptibility. | Marcela Alcantara Proenca Juliana Garcia de Oliveira Aline Cristina Targa Cadamuro Maysa Succi Joao Gomes Netinho Eny Maria Goloni-Bertolo érika Cristina Pavarino Ana Elizabete Silva | 2015 | World Journal of Gastroenterology2015，21，25： | 9 |
| 3 | Molecular detection of monocyte chemotactic protein-1 polymorphism in spontaneous bacterial peritonitis patients显示文摘AIM: To investigate the association of the functional monocyte chemotactic protein-1(MCP-1) promoter polymorphism(A-2518G) with spontaneous bacterial peritonitis(SBP).METHODS: Fifty patients with post-hepatitis C liver cirrhosis and ascites were categorized into two groups; group Ⅰ included 25 patients with SBP and group Ⅱ included 25 patients free from SBP. In addition, a group of 20 healthy volunteers were included. We assessed the MCP-1 gene polymorphism and gene expression as well as interleukin(IL)-10 levels in both blood and ascitic fluid. RESULTS: A significant MCP-1 gene polymorphism was detected in groups Ⅰ and Ⅱ(P = 0.001 and 0.02 respectively). Group Ⅰ was associated with a significantly higher frequency of AG genotype [control 8(40%) vs SBP 19(76.0%), P < 0.001], and group Ⅱ was associated with a significantly higher frequency of GG genotype when compared to healthy volunteers [control 1(5%) vs cirrhotic 16(64%), P < 0.001]. Accordingly, the frequency of G allele was significantly higher in both groups(Ⅰ and Ⅱ) [control 10(25%) vs SBP 27(54%), P < 0.001 and vs cirrhotic 37(74.0%), P < 0.001, respectively]. The total blood and ascetic fluid levels of IL-10 and MCP-1 gene expression were significantly higher in group Ⅰ than in group Ⅱ. Group Ⅰ showed significant reductions in the levels of MCP-1 gene expression and IL-10 in the whole blood and ascetic fluid after therapy. CONCLUSION: MCP-1 GG genotype and G allele may predispose HCV infected patients to a more progressive disease course, while AG genotype may increase the susceptibility to SBP. Patients carrying these genotypes should be under supervision to prevent or restrict further complications. | Maysa Kamal Salama Dina Sabry Mohamed AS Al-Ghussein Rasha Ahmed Sayed AbdAllah Fatma Mohamed Taha Wael Fathy Miriam Safwat Wadie Mona Nabih Amr Abul-Fotouh Tarneem Darwish | 2014 | World Journal of Gastroenterology2014，20，33： | 7 |
| 4 | Prognostic markers in triple‐negative breast cancer显示文摘 | Emad A.Rakha Maysa E.El‐Sayed Andrew R.Green Andrew H. S.Lee John F.Robertson Ian O.Ellis | 2006 | Cancer2006，，1： | 4 |
| 5 | Prognostic markers in triple‐negative breast cancer显示文摘 | Emad A.Rakha Maysa E.El‐Sayed Andrew R.Green Andrew H. S.Lee John F.Robertson Ian O.Ellis | 2006 | Cancer2006，，1： | 2 |
| 6 | Recreational team sports: The motivational medicine显示文摘Remember way back when?When we were kids?We played games like soccer,basketball,rugby,and volleyball because they were fun.If we stayed with it long enough(and became good enough)we might have played competitively,where the objective was to win.Herman Edwards,a former head coach in the National Football League(NFL)。 | Carlo Castagna Maysa de Sousa Peter Krustrup Donald T.Kirkendall | 2018 | Journal of Sport and Health Science2018，7，2： | 2 |
| 7 | The interplay of language on execu- tive functions in children with ASD 显示文摘 | Maysa A Rebecca L Rhea P | 2013 | Res Autism Spect Disord2013，7，： | 1 |
| 8 | Purification and characterization of milk dotting enzyme produced by Bacillus sphaer/cus显示文摘 | Magada A Bendary E L Maysa E M | 2007 | Journal of Applied Sciences Research2007，3，8： | 1 |
| 9 | Purification and characterization of milk clotting enzyme produced by Bacillus sphaericus 显示文摘 | MAGADA A BENDARY E L MAYSA E M | 2007 | Journal of Applied Sciences Research2007，3，8： | 1 |
| 10 | Pineal gland turners: Experience from the SEER database 显示文摘 | Maysa AH Iyad S Amar JG | 2009 | J Neurooncol2009，94，3： | 1 |
| 11 | An adaptive chart for monitoring the process mean and variance 显示文摘 | Costa A F B Maysa S De Magalhaes | 2007 | Quality and Reliability Engineering International2007，23，： | 1 |
| 12 | Undifferentiated endometrial carcinoma: a diagnosis frequently overlooked 显示文摘 | AI-Loh Shaymaa Al-Hussaini Maysa | 2013 | Arch Pathol Lab Med2013，137，3： | 1 |
| 13 | Effect of alpha prime due to 475?°C aging on fracture behavior and corrosion resistance of DIN 1.4575 and MA 956 high performance ferritic stainless steels显示文摘 | Maysa Terada Marcio F. Hupalo Isolda Costa Angelo F. Padilha | 2008 | Journal of Materials Science2008，，2： | 1 |
| 14 | Application of PCR ribotyping and tDNA-PCR for Klebsiella pneumoniae identification显示文摘 | Ana Catarina S L Juliana F R Maysa B M C | 2007 | Mere Inst Oswaldo Cruz Rio de Janeiro2007，102，7： | 1 |
| 15 | Purification and characterization of milk clotting enzyme produced by Bacillus sphaericus显示文摘 | MAGADA A BENDARY E L MAYSA E M | 2007 | Journal of Applied Sciences Research2007，3，8： | 1 |
| 16 | The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis显示文摘 | Camila O. Carvalho Ana Carolina S. Chagas Fernando Cotinguiba Maysa Furlan Luciana G. Brito Francisco C.M. Chaves Marília P. Stephan Humberto R. Bizzo Alessandro F.T. Amarante | 2011 | Veterinary Parasitology2011，，3： | 1 |
| 17 | Comparison between Pernasal Flocked Swabs and Nasopharyngeal Aspirates for Detection of Common Respiratory Viruses in Samples from Children显示文摘 | Afaf AD Maysa A Raed G | 2008 | J Clin Microbiol2008，46，： | 1 |
| 18 | Prognostic markers in Triple-negative breast cancer显示文摘 | Emad A Maysa E Andrew R | 2007 | Cancer2007，109，1： | 1 |
| 19 | Purification and characterization of milk clotting enzyme produced by Bacillus sphaericus显示文摘 | EL-BENDARY M A MAYSA E M THANAA H A | 2007 | J Appl Sci Res2007，3，8： | 1 |
| 20 | Prognostic markers in triple‐negative breast cancer显示文摘 | Emad A.Rakha Maysa E.El‐Sayed Andrew R.Green Andrew H. S.Lee John F.Robertson Ian O.Ellis | 2006 | Cancer2006，，1： | 1 |
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