|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Lymphocyte subsets in alcoholic liver disease显示文摘AIM:To compare lymphocyte subsets between healthy controls and alcoholics with liver disease.METHODS:The patient cohort for this study included individuals who were suspected to have alcoholic liver disease(ALD) and who had undergone liver biopsy(for disease grading and staging,doubts about diagnosis,or concurrent liver disease;n = 56).Normal controls included patients who were admitted for elective cholecystectomy due to non-complicated gallstones(n = 27).Formalin-fixed,paraffin-embedded liver biopsy specimens were sectioned and stained with hematoxylin and eosin and Perls' Prussian blue.The non-alcoholic steatohepatitis score was used to assess markers of ALD.Lymphocyte population subsets were determined by flow cytometry.T lymphocytes were identified(CD3+),and then further subdivided into CD4+ or CD8+ populations.B lymphocytes(CD19+) and natural killer(NK) cell numbers were also measured.In addition to assessing lymphocyte subpopulation differences between ALD patients and controls,we also compared subsets of alcoholic patients without cirrhosis or abstinent cirrhotic patients to normal controls.RESULTS:The patient cohort primarily consisted of older men.Active alcoholism was present in 66.1%.Reported average daily alcohol intake was 164.9 g and the average lifetime cumulative intake was 2211.6 kg.Cirrhosis was present in 39.3% of the patients and 66.1% had significant fibrosis(perisinusoidal and portal/periportal fibrosis,bridging fibrosis,or cirrhosis) in their liver samples.The average Mayo end-stage liver disease score was 7.6.No hereditary hemochromatosis genotypes were found.ALD patients(n = 56) presented with significant lymphopenia(1.5 × 109/L ± 0.5 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P < 0.0001),due to a decrease in all lymphocyte subpopulations,except for NK lymphocytes:CD3+(1013.0 ± 406.2/mm3 vs 1523.0 ± 364.6/mm3,P < 0.0001),CD4+(713.5 ± 284.7/mm3 vs 992.4 ± 274.7/mm3,P < 0.0001),CD8+(262.3 ± 140.4/mm3 vs 478.9 ± 164.6/mm3,P < 0.0001),and CD19+(120.6 ± 76.1/mm3 vs 264.6 ± 88.0/mm3,P < 0.0001).CD8+ lymphocytes suffered the greatest reduction,as evidenced by an increase in the CD4+/CD8+ ratio(3.1 ± 1.3 vs 2.3 ± 0.9,P = 0.013).This ratio was associated with the stage of fibrosis on liver biopsy(rs = 0.342,P = 0.01) and with Child-Pugh score(rs = 0.482,P = 0.02).The number of CD8+ lymphocytes also had a positive association with serum ferritin levels(rs = 0.345,P = 0.009).Considering only patients with active alcoholism but not cirrhosis(n = 27),we found similar reductions in total lymphocyte counts(1.8 × 109/L ± 0.3 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P = 0.018),and in populations of CD3+(1164.7 ± 376.6/mm3 vs 1523.0 ± 364.6/mm3,P = 0.001),CD4+(759.8 ± 265.0/mm3 vs 992.4 ± 274.7/mm3,P = 0.003),CD8+(330.9 ± 156.3/mm3 vs 478.9 ± 164.6/mm3,P = 0.002),and CD19+(108.8 ± 64.2/mm3 vs 264.6 ± 88.0/mm3,P < 0.0001).In these patients,the CD4+/CD8+ ratio and the number of NK lymphocytes was not significantly different,compared to controls.Comparing patients with liver cirrhosis but without active alcohol consumption(n = 11),we also found significant lymphopenia(1.3 × 109/L ± 0.6 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P < 0.0001) and decreases in populations of CD3+(945.5 ± 547.4/mm3 vs 1523.0 ± 364.6/mm3,P = 0.003),CD4+(745.2 ± 389.0/mm3 vs 992.4 ± 274.7/mm3,P = 0.032),CD8+(233.9 ± 120.0/mm3 vs 478.9 ± 164.6/mm3,P < 0.0001),and CD19+(150.8 ± 76.1/mm3 vs 264.6 ± 88.0/mm3,P = 0.001).The NK lymphocyte count was not significantly different,but,in this group,there was a significant increase in the CD4+/CD8+ ratio(3.5 ± 1.3 vs 2.3 ± 0.9,P = 0.01).CONCLUSION:All patient subsets presented with decreased lymphocyte counts,but only patients with advanced fibrosis presented with a significant increase in the CD4+/CD8+ ratio. | Luís Costa Matos Paulo Batista Nuno Monteiro Joo Ribeiro Maria A Cipriano Pedro Henriques Fernando Giro Armando Carvalho | 2013 | World Journal of Hepatology2013，5，2： | 4 |
| 2 | Pancreatic acinar cell carcinoma: a multi-institutional study 显示文摘 | Matos J M Schmidt C M Turrini O | 2009 | J Gastrointest Surg2009，13，8： | 1 |
| 3 | Identification of a new microsporidian parasite related to Vittaforma corneae in HIV-positire and HIV-negative patients from Portugal显示文摘 | Sulaiman IM Matos O Lobo ML | 2003 | J Eukaryot Microbiol2003，，： | 1 |
| 4 | Aggressive angiomyxoma of vulva显示文摘 | Jardin O Matos R Falcao F | 2001 | Acta Med Port2001，14，： | 1 |
| 5 | Simultaneous follow-up of mouse colon lesions by colonoscopy and endoluminal ultrasound biomicroscopy显示文摘AIM:To evaluate the potential use of colonoscopy and endoluminal ultrasonic biomicroscopy(eUBM)to track the progression of mouse colonic lesions.METHODS:Ten mice were treated with a single azoxy-methane intraperitoneal injection(week 1)followed by seven days of a dextran sulfate sodium treatment in their drinking water(week 2)to induce inflammationassociated colon tumors.eUBM was performed simultaneously with colonoscopy at weeks 13,17-20 and21.A 3.6-F diameter 40 MHz mini-probe catheter was used for eUBM imaging.The ultrasound mini-probe catheter was inserted into the accessory channel of a pediatric flexible bronchofiberscope,allowing simultaneous acquisition of colonoscopic and eUBM images.During image acquisition,the mice were anesthetized with isoflurane and kept in a supine position over a stainless steel heated surgical waterbed at 37℃.Both eUBM and colonoscopic images were captured and stored when a lesion was detected by colonoscopy or when the eUBM image revealed a modified colon wall anatomy.During the procedure,the colon was irrigated with water that was injected through a flush port on the mini-probe catheter and that acted as the ultrasound coupling medium between the transducer and the colon wall.Once the acquisition of the last eUBM/colonoscopy section for each animal was completed,the colons were fixed,paraffin-embedded,and stained with hematoxylin and eosin.Colon images acquired at the first time-point for each mouse were compared with subsequent eUBM/colonoscopic images of the same sites obtained in the following acquisitions to evaluate lesion progression.RESULTS:All 10 mice had eUBM and colonoscopic images acquired at week 13(the first time-point).Two animals died immediately after the first imaging acquisition and,consequently,only 8 mice were subjected to the second eUBM/colonoscopy imaging acquisition(at the second time-point).Due to the advanced stage of colonic tumorigenesis,5 animals died after the second time-point image acquisition,and thus,only three were subjected to the third eUBM/colonoscopy imaging acquisition(the third time-point).eUBM was able to detect the four layers in healthy segments of colon:the mucosa(the first hyperechoic layer moving away from the mini-probe axis),followed by the muscularis mucosae(hypoechoic),the submucosa(the second hyperechoic layer)and the muscularis externa(the second hypoechoic layer).Hypoechoic regions between the mucosa and the muscularis externa layers represented lymphoid infiltrates,as confirmed by the corresponding histological images.Pedunculated tumors were represented by hyperechoic masses in the mucosa layer.Among the lesions that decreased in size between the first and third time-points,one of the lesions changed from a mucosal hyperplasia with ulceration at the top to a mucosal hyperplasia with lymphoid infiltrate and,finally,to small signs of mucosal hyperplasia and lymphoid infiltrate.In this case,while lesion regression and modification were observable in the eUBM images,colonoscopy was only able to detect the lesion at the first and second time-points,without the capacity to demonstrate the presence of lymphoid infiltrate.Regarding the lesions that increased in size,one of them started as a small elevation in the mucosa layer and progressed to a pedunculated tumor.In this case,while eUBM imaging revealed the lesion at the first time-point,colonoscopy was only able to detect it at the second time-point.All colonic lesions(tumors,lymphoid infiltrate and mucosal thickening)were identified by eUBM,while colonoscopy identified just76%of them.Colonoscopy identified all of the colonic tumors but failed to diagnose lymphoid infiltrates and increased mucosal thickness and failed to differentiate lymphoid infiltrates from small adenomas.During the observation period,most of the lesions(approximately67%)increased in size,approximately 14%remained unchanged,and 19%regressed.CONCLUSION:Combining eUBM with colonoscopy improves the diagnosis and the follow-up of mouse colonic lesions,adding transmural assessment of the bowel wall. | Rossana C Soletti Kelly Z Alves Marcelo AP de Britto Dyanna G de Matos Mnica Soldan Helena L Borges Joo C Machado | 2013 | World Journal of Gastroenterology2013，19，44： | 1 |
| 6 | Tumor thickness as a predictive factor of lymph node metastasis and disease recurrence in T1N0 and T2N0 squamous cell carcinoma of the oral tongue显示文摘 | Leandro Luongo de Matos Gabriel Manfro Ricardo Vieira dos Santos Elaine Stabenow Evandro Sobroza de Mello Venancio Avancini F. Alves Fábio Roberto Pinto Marco Aurélio Vamondes Kulcsar Lenine Garcia Brand?o Cláudio Roberto Cernea | 2014 | Oral Surgery Oral Medicine Oral Pathology and Oral Radiology2014，，： | 1 |
| 7 | Effect of sodium dodecylsulfate and benzotriazole on the interracial behavior of Cu/Cu(Ⅱ), H2SO4显示文摘 | Villamil R F V Cordeiro G G O Matos J | 2002 | Materials Chemistry and Physics2002，，78： | 1 |
| 8 | Effect of specific exercise training on bone mineral density in women with postmenopausal osteopenia or osteoporosis显示文摘 | de Matos O Lopes da Silva DJ Martinez de Oliveira J | | 0，，09： | 1 |
| 9 | Dramatic change in Citrus tristeza virus populations in the Dominican Republic显示文摘 | MATOS L A HILF M E CAYETANO X A FELIZ A O HARPER S J FOLIMONOVA S Y | 2013 | Plant Disease2013，97，： | 1 |
| 10 | Multiperiod synthesis and operational planning of utility systems with environmental concerns 显示文摘 | FRANCISCO O A P MATOS H A | 2004 | Computers and Chemical Engineering2004，28，5： | 1 |
| 11 | Targeting Leishmania ma- jor antigens to dendritic cells in vivo induces protective immunity 显示文摘 | Matos I Mizenina O Lubkin A | 2013 | Immunol Res2013，26，6： | 1 |
| 12 | Structural Assessment Under Uncertain Parameters via Interval Analysis显示文摘 | GARCIA O VEHI J MATOS J C | 2008 | Journal of Computation and Applied Mathematics2008，218，1： | 1 |
| 13 | Multilocus PCR-RFLP analysis of Cryptosporidium isolates from HIV-infected patients from Portugal显示文摘 | Alves M Matos O Antunes F | 2001 | Ann Trop Med Parasitol2001，95，6： | 1 |
| 14 | Pancreas divisum: evaluation with secretin-enhanced magnetic resonance cholangiopancreatography显示文摘 | Matos C Metens T Deviere J Delhaye M Le Moine O Cremer M | 2001 | Gastrointest Endose2001，53，： | 1 |
| 15 | Effect of specific exercise Raining on bone mineral density in women with postmenopausal osteopenia or osteoporosis显示文摘 | de Matos O Lopes da Silva DJ Martinez de Oliveira J | 2009 | Gynecol Endocrinol2009，25，9： | 1 |
| 16 | Rescaled range analysis and detrended fluctuation analysis study of cast irons ultrasonic backscattered signals 显示文摘 | MAURICIO J MATOS O ELINEUDO P MOURA de SILVIO KRUGER E MACCOS J REBELLO A | 2004 | CHAOS SOLITIONS & FRACTALS2004，，19： | 1 |
| 17 | Liver Retransplantation: A Model for Determining Long-Term Survival显示文摘 | Marcelo M. Linhares Daniel Azoulay Délcio Matos Adauto Castelo-Filho Tarcísio Trivi?o Alberto Goldenberg Denis Castaing René Adam Valerie Délvart Phillipe Ichai Fauze Saliba Antoine Lemoine Didier Samuel Henry Bismuth | 2006 | Transplantation2006，，7： | 1 |
| 18 | Esterification of Oleic Acid Using 12-Tungstophosphoric Supported in Flint Kaolin of the Amazonia显示文摘 | Júnior O D S L Cavalcanti R M Matos T M D | 2013 | Fuel2013，108，： | 1 |
| 19 | Expression of ck 19, galectin-3 and hbme-1 in the differentiation of thyroid lesions : systematic review and diagnostic meta-analysis 显示文摘 | Matos L L Del Giglio A B Matsubayashi C O | 2012 | Diagn Pathol2012，7，1： | 1 |
| 20 | Pancreatic acinar cell carcinoma: a Multi-institutional study显示文摘 | Matos JM Schmidt CM Turrini O | 2009 | J Gastrointest Surg2009，13，8： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员