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| 1 | Colon cancer-associated B2 Escherichia coli colonize gut mucosa and promote cell proliferation显示文摘AIM:To provide further insight into the characterization of mucosa-associated Escherichia coli(E.coli)isolated from the colonic mucosa of cancer patients.METHODS:Phylogroups and the presence of cyclomodulin-encoding genes of mucosa-associated E.coli from colon cancer and diverticulosis specimens weredetermined by PCR.Adhesion and invasion experiments were performed with I-407 intestinal epithelial cells using gentamicin protection assay.Carcinoembryonic antigen-related cell adhesion molecule 6(CEACAM6)expression in T84 intestinal epithelial cells was measured by enzyme-linked immunosorbent assay and by Western Blot.Gut colonization,inflammation and procarcinogenic potential were assessed in a chronic infection model using CEABAC10 transgenic mice.Cell proliferation was analyzed by real-time mRNA quantification of PCNA and immunohistochemistry staining of Ki67.RESULTS:Analysis of mucosa-associated E.coli from colon cancer and diverticulosis specimens showed that whatever the origin of the E.coli strains,86%of cyclomodulin-positive E.coli belonged to B2 phylogroup and most harbored polyketide synthase(pks)island,which encodes colibactin,and/or cytotoxic necrotizing factor(cnf)genes.In vitro assays using I-407 intestinal epithelial cells revealed that mucosa-associated B2 E.coli strains were poorly adherent and invasive.However,mucosa-associated B2 E.coli similarly to Crohn’s disease-associated E.coli are able to induce CEACAM6expression in T84 intestinal epithelial cells.In addition,in vivo experiments using a chronic infection model of CEACAM6 expressing mice showed that B2 E.coli strain11G5 isolated from colon cancer is able to highly persist in the gut,and to induce colon inflammation,epithelial damages and cell proliferation.CONCLUSION:In conclusion,these data bring new insights into the ability of E.coli isolated from patients with colon cancer to establish persistent colonization,exacerbate inflammation and trigger carcinogenesis. | Jennifer Raisch Emmanuel Buc Mathilde Bonnet Pierre Sauvanet Emilie Vazeille Amélie de Vallée Pierre Déchelotte Claude Darcha Denis Pezet Richard Bonnet Marie-Agnès Bringer Arlette Darfeuille-Michaud | 2014 | World Journal of Gastroenterology2014，20，21： | 12 |
| 2 | Karyotype and Genome Size Estimation of Haliotis midae: Estimators to Assist Future Studies on the Evolutionary History of Haliotidae显示文摘 | PAOLO F RUHAN S MATHILDE V D M | 2010 | Journal of Shellfish Research2010，29，4： | 1 |
| 3 | Animal models of myasthenia gravis显示文摘 | Mathilde P Caishu D | 2000 | Clin Immunol2000，94，2： | 1 |
| 4 | The Putative Tumor Suppressor Gene PTPN13 Induces Apoptosis through Insulin Receptor Substrate-1 Dephosphorylation显示文摘 | Mathilde D Guillaume B Murielle GL | 2007 | Cancer Res2007，67，： | 1 |
| 5 | Monitoring Quality in Work:European Employment Strategy Indicators and Beyond显示文摘 | Lucie D Christine E Mathilde G L | | 0，，02： | 1 |
| 6 | Glucotoxicity inhibits late steps of insulin exocytosis显示文摘 | Mathilde D Pierre V Benoit R | 2007 | Endocrinology2007，148，4： | 1 |
| 7 | Heat Shock Proteins 27 and 70 Anti-Apoptotic Proteins with Tumorigenic Properties 显示文摘 | Carmen G Mathilde B Celine D et a | 2006 | Cell Cycle2006，5，22： | 1 |
| 8 | Chronic exposure to uranium leads to iron accumulation in rat kidney cells显示文摘 | Marie DC Mathilde B Bernadette D | 2007 | Radiat Res2007，167，4： | 1 |
| 9 | Heat shock proteins 27 and 70 anti-apoptotic proteins with tumorigenic properties显示文摘 | Carmen G Mathilde B Celine D | | 0，，22： | 1 |
| 10 | There is more to tomato fruit colour than candidate carotenoid genes 显示文摘 | Liu Yongsheng Amit G Gil R Mathilde C Rene D Michel B Joseph H Dani Z | 2003 | Plant Biotechnology Journal2003，1，： | 1 |
| 11 | Animal models of myasthenia gravis显示文摘 | Pyemkumar C Mathilde P Caishu D | 2000 | Clin Immunol2000，94，2： | 1 |
| 12 | Animal models of myasthenia gravis 显示文摘 | Premkumar C Mathilde P Caishu D | 2000 | Clin Immunol2000，94，2： | 1 |
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